Reviewed, UniProtKB/Swiss-Prot P35335 (PGLR_ASPOR)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase

Gene names

ORF Names:

AO090023000161

Organism

Aspergillus oryzae [Complete proteome]

Taxonomic identifier

5062 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 6 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 28	8	Potential		PRO_0000024780
	Chain	29 – 363	335	Polygalacturonase		PRO_0000024781
Sites
	Active site	202	1	Proton donor By similarity
	Active site	224	1	By similarity
Amino acid modifications
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 46		By similarity
	Disulfide bond	204 ↔ 220		By similarity
	Disulfide bond	330 ↔ 335		By similarity
	Disulfide bond	354 ↔ 363		By similarity
Experimental info
	Sequence conflict	337 – 338	2	DW → GL in BAA03244. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35335-1 [UniParc]. Last modified May 2, 2006. Version 2. Checksum: 83DC803AE2865504	FASTA	363	37,651
	10 20 30 40 50 60 MQLLQSSVIA ATVGAALVAA VPVELKARDS CTFTSAADAK SGKTSCSTIT LSNIEVPAGE 70 80 90 100 110 120 TLDLTGLNDG TTVIFSGETT FGYKEWEGPL ISVSGTNIKV QQASGAKIDG DGSRWWDGKG 130 140 150 160 170 180 GNGGKTKPKF FYAHKLDSSS ITGLQIYNTP VQGFSIQSDN LNITDVTIDN SAGTAEGHNT 190 200 210 220 230 240 DAFDVGSSTY INIDGATVYN QDDCLAINSG SHITFTNGYC DGGHGLSIGS VGGRSDNTVE 250 260 270 280 290 300 DVTISNSKVV NSQNGVRIKT VYDATGTVSN VKFEDITLSG ITKYGLIVEQ DYENGSPTGT 310 320 330 340 350 360 PTNGIKVSDI TFDKVTGTVE SDATDIYILC GSGSCTDWTW SGVSITGGKT SSKCENVSTG ASC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616." Kitamoto N., Kimura T., Kito Y., Ohmiya K., Tsukagoshi N. FEMS Microbiol. Lett. 111:37-42(1993) [PubMed: 8359678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KBN616.
[2]	"Genome sequencing and analysis of Aspergillus oryzae." Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K., Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H., Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E. , Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D., Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A., Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y., Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H., Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T., Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O., Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y., Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N., Kikuchi H. Nature 438:1157-1161(2005) [PubMed: 16372010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 42149 / RIB 40.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D14282 Genomic DNA. Translation: BAA03244.1. AP007157 Genomic DNA. Translation: BAE58737.1.
RefSeq	XP_001820739.1.
3D structure databases
HSSP	HSSP built from PDB template 1IB4 based on UniProtKB O74213.
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Genome annotation databases
GeneID	5992741.
KEGG	aor:AO090023000161.
Enzyme and pathway databases
BRENDA	3.2.1.15. 2240.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 6 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_ASPOR

Accession

Primary (citable) accession number: P35335
Secondary accession number(s): Q2UI78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 2, 2006
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)