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Protein

B-cell receptor CD22

Gene

Cd22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124Sialic acidBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: UniProtKB-KW
  • coreceptor activity Source: MGI

GO - Biological processi

  • cell adhesion Source: UniProtKB-KW
  • cell surface receptor signaling pathway Source: MGI

Keywordsi

Biological processCell adhesion
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
B-cell receptor CD22
Alternative name(s):
B-lymphocyte cell adhesion molecule
Short name:
BL-CAM
Sialic acid-binding Ig-like lectin 2
Short name:
Siglec-2
T-cell surface antigen Leu-14
CD_antigen: CD22
Gene namesi
Name:Cd22
Synonyms:Lyb-8, Siglec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88322. Cd22.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 702ExtracellularSequence analysisAdd BLAST681
Transmembranei703 – 721HelicalSequence analysisAdd BLAST19
Topological domaini722 – 862CytoplasmicSequence analysisAdd BLAST141

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi822Y → F: Abolishes binding to GRB2. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1075279.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001487422 – 862B-cell receptor CD22Add BLAST841

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 171PROSITE-ProRule annotation
Disulfide bondi46 ↔ 106PROSITE-ProRule annotation
Glycosylationi105N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi139N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi165 ↔ 229PROSITE-ProRule annotation
Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi265N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi272 ↔ 324PROSITE-ProRule annotation
Glycosylationi275N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi368 ↔ 411PROSITE-ProRule annotation
Glycosylationi378N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi408N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi457 ↔ 499PROSITE-ProRule annotation
Glycosylationi460N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi544 ↔ 586PROSITE-ProRule annotation
Glycosylationi561N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi589N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi631 ↔ 674PROSITE-ProRule annotation
Modified residuei740PhosphoserineCombined sources1
Modified residuei741PhosphoserineCombined sources1
Modified residuei744PhosphoserineCombined sources1
Modified residuei777Phosphotyrosine1 Publication1
Modified residuei822Phosphotyrosine1 Publication1
Modified residuei837Phosphotyrosine1 Publication1
Modified residuei857Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated on tyrosine residues by LYN.Curated
Phosphorylation of Tyr-777 and Tyr-837 are involved in binding to SYK. Phosphorylation of Tyr-822 is involved in binding to GRB2. Phosphorylation of Tyr-857 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35329.
PaxDbiP35329.
PRIDEiP35329.

PTM databases

iPTMnetiP35329.
PhosphoSitePlusiP35329.

Expressioni

Tissue specificityi

B-lymphocytes.

Gene expression databases

CleanExiMM_CD22.

Interactioni

Subunit structurei

Interacts with LYN, SYK, PIK3R1/PIK3R2, PLCG1, SHC1, INPP5D and GRB2 upon phosphorylation. May form a complex with INPP5D/SHIP, GRB2 and SHC1. Interacts with PTPN6/SHP-1 upon phosphorylation (By similarity).By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

CORUMiP35329.
IntActiP35329. 8 interactors.
MINTiMINT-1659949.
STRINGi10090.ENSMUSP00000019248.

Chemistry databases

BindingDBiP35329.

Structurei

3D structure databases

ProteinModelPortaliP35329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 142Ig-like V-typeAdd BLAST121
Domaini147 – 244Ig-like C2-type 1Add BLAST98
Domaini251 – 341Ig-like C2-type 2Add BLAST91
Domaini346 – 429Ig-like C2-type 3Add BLAST84
Domaini434 – 515Ig-like C2-type 4Add BLAST82
Domaini520 – 597Ig-like C2-type 5Add BLAST78
Domaini608 – 691Ig-like C2-type 6Add BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi775 – 780ITIM motif 16
Motifi835 – 840ITIM motif 26
Motifi855 – 860ITIM motif 36

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IUGI. Eukaryota.
ENOG410YH8H. LUCA.
HOVERGENiHBG005272.
InParanoidiP35329.
KOiK06467.
PhylomeDBiP35329.

Family and domain databases

Gene3Di2.60.40.10. 7 hits.
InterProiView protein in InterPro
IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
PfamiView protein in Pfam
PF08205. C2-set_2. 1 hit.
PF13895. Ig_2. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 7 hits.
SM00408. IGc2. 5 hits.
SUPFAMiSSF48726. SSF48726. 7 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 6 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35329-1) [UniParc]FASTAAdd to basket
Also known as: CD22-beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVHYLWLLL ILGHAASAQY SSANDWTVDH PQTLFAWEGA CIRIPCKYKT
60 70 80 90 100
PLPKARLDNI LLFQNYEFDK ATKKFKGTVL YNKAEPELYP PKQRRVTFLG
110 120 130 140 150
NSIDNCTLKI HPIRANDSGN LGLRMTAGTE RWMEPIHLNV SEKPFQPYIQ
160 170 180 190 200
MPSEIRESQS VTLTCGLNFS CFEYDILLQW FLEDSKITSV TPSVTSITSS
210 220 230 240 250
VTSSIKNVYT ESKLTFQPKW TDHGKSVKCQ VQHSSEVLSE RTVRLDVKYT
260 270 280 290 300
PKLEIKVNPT EVEKNNSVTM TCRVNSSNPK LRTVAVSWFK DGRPLEDQEL
310 320 330 340 350
EQEQQMSKLI LHSVTKDMRG KYRCQASNDI GPGESEEVEL TVHYAPEPSR
360 370 380 390 400
VHIYPSPAEE GQSVELICES LASPSATNYT WYHNRKPIPG DTQEKLRIPK
410 420 430 440 450
VSPWHAGNYS CLAENRLGHG KIDQEAKLDV HYAPKAVTTV IQSFTPILEG
460 470 480 490 500
DSVTLVCRYN SSNPDVTSYR WNPQGSGSVL KPGVLRIQKV TWDSMPVSCA
510 520 530 540 550
ACNHKCSWAL PVILNVHYAP RDVKVLKVSP ASEIRAGQRV LLQCDFAESN
560 570 580 590 600
PAEVRFFWKK NGSLVQEGRY LSFGSVSPED SGNYNCMVNN SIGETLSQAW
610 620 630 640 650
NLQVLYAPRR LRVSISPGDH VMEGKKATLS CESDANPPIS QYTWFDSSGQ
660 670 680 690 700
DLHSSGQKLR LEPLEVQHTG SYRCKGTNGI GTGESPPSTL TVYYSPETIG
710 720 730 740 750
KRVALGLGFC LTICILAIWG MKIQKKWKQN RSQQGLQENS SGQSFFVRNK
760 770 780 790 800
KARRTPLSEG PQSQGCYNPA MDDTVSYAIL RFPESDMHNA GDAGTPATQA
810 820 830 840 850
PPPNNSDSVT YSVIQKRPMG DYENVNPSCP EDESIHYSEL VQFGAGKRPQ
860
AKEDVDYVTL KH
Length:862
Mass (Da):96,582
Last modified:February 1, 1994 - v1
Checksum:iA7662D6E87038E83
GO
Isoform 2 (identifier: P35329-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-115: Missing.

Show »
Length:800
Mass (Da):89,333
Checksum:i394094553D0F14E6
GO
Isoform 3 (identifier: P35329-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-133: Missing.

Show »
Length:828
Mass (Da):92,857
Checksum:i6C84F4BE3F0B6045
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87E → G in CAB85611 (PubMed:10975807).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti15A → V in strain: BALB/c, C57BL/6, BXSB and MRL. 1
Natural varianti19Q → R in strain: BALB/c, C57BL/6, BXSB and MRL. 1
Natural varianti76K → T in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti83 – 86KAEP → ATKTEKDPES in strain: BALB/c, C57BL/6, BXSB and MRL. 4
Natural varianti90 – 91PP → LS in strain: BALB/c. 2
Natural varianti94R → G in strain: BALB/c. 1
Natural varianti102S → R in strain: BALB/c, C57BL/6, BXSB and MRL. 1
Natural varianti112 – 114PIR → LIH in strain: BXSB. 3
Natural varianti173E → G in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti179Q → K in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti186K → E in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti190V → I in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti192P → S in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti236E → K in strain: BALB/c, C57BL/6 and MRL. 1
Natural varianti241R → C in strain: BALB/c. 1
Natural varianti244R → H in strain: BALB/c and MRL. 1
Natural varianti273R → Q in strain: BXSB. 1
Natural varianti380T → I in strain: BXSB. 1
Natural varianti387P → H in strain: BXSB. 1
Natural varianti403P → L in strain: BXSB. 1
Natural varianti419H → R in strain: BXSB. 1
Natural varianti423D → G in strain: BXSB. 1
Natural varianti482P → S in strain: BXSB. 1
Natural varianti548E → K in strain: BXSB. 1
Natural varianti616S → I in strain: BALB/c. 1
Natural varianti620H → R in strain: BXSB. 1
Natural varianti714C → F in strain: BALB/c. 1
Natural varianti787M → T in strain: BALB/c, C57BL/6, BXSB and MRL. 1
Natural varianti790A → T in strain: C57BL/6. 1
Natural varianti808S → T in strain: BALB/c, BXSB and MRL. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00253254 – 115Missing in isoform 2. 1 PublicationAdd BLAST62
Alternative sequenceiVSP_002533100 – 133Missing in isoform 3. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L16928 mRNA. Translation: AAA02562.1.
AF115401 mRNA. Translation: AAD30392.1.
AF115400 mRNA. Translation: AAD30391.1.
AF102134 mRNA. Translation: AAF02417.1.
AJ250676 mRNA. Translation: CAB85609.1.
AJ250677 mRNA. Translation: CAB85610.1.
AJ250678 mRNA. Translation: CAB85611.1.
AJ250679 mRNA. Translation: CAB85612.1.
AJ250680 mRNA. Translation: CAB85613.1.
AJ250682 mRNA. Translation: CAB85615.1.
AJ250683 mRNA. Translation: CAB85616.1.
PIRiA46512.
I49583.
RefSeqiNP_001036782.1. NM_001043317.2.
NP_033975.3. NM_009845.3.
XP_006539556.1. XM_006539493.1.
XP_006539557.1. XM_006539494.2.
UniGeneiMm.260994.

Genome annotation databases

GeneIDi12483.
KEGGimmu:12483.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCD22_MOUSE
AccessioniPrimary (citable) accession number: P35329
Secondary accession number(s): Q9JHL2
, Q9JJX9, Q9JJY0, Q9JJY1, Q9R056, Q9R094, Q9WU51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 22, 2017
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families