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Protein

Acyl-homoserine-lactone synthase

Gene

luxI

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone) also known as VAI or N-(beta-ketocaproyl)homoserine lactone or 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)-hexanamide, an autoinducer molecule which binds to LuxR and thus acts in bioluminescence regulation.

Catalytic activityi

An acyl-[acyl-carrier-protein] + S-adenosyl-L-methionine = [acyl-carrier-protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Autoinducer synthesis, Luminescence, Quorum sensing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-3681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-homoserine-lactone synthase (EC:2.3.1.184)
Alternative name(s):
Autoinducer synthesis protein LuxI
Gene namesi
Name:luxI
Ordered Locus Names:VF_A0924
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
Proteomesi
  • UP000000537 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Acyl-homoserine-lactone synthasePRO_0000210889Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi312309.VF_A0924.

Structurei

3D structure databases

ProteinModelPortaliP35328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the autoinducer synthase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105NNS. Bacteria.
COG3916. LUCA.
HOGENOMiHOG000207772.
KOiK13060.
OrthoDBiEOG60GRTV.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR018311. Autoind_synth_CS.
IPR001690. Autoind_synthase.
[Graphical view]
PfamiPF00765. Autoind_synth. 1 hit.
[Graphical view]
PRINTSiPR01549. AUTOINDCRSYN.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS00949. AUTOINDUCER_SYNTH_1. 1 hit.
PS51187. AUTOINDUCER_SYNTH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVMIKKSDF LGIPSEEYRG ILSLRYQVFK RRLEWDLVSE DNLESDEYDN
60 70 80 90 100
SNAEYIYACD DAEEVNGCWR LLPTTGDYML KTVFPELLGD QVAPRDPNIV
110 120 130 140 150
ELSRFAVGKN SSKINNSASE ITMKLFQAIY KHAVSQGITE YVTVTSIAIE
160 170 180 190
RFLKRIKVPC HRIGDKEIHL LGNTRSVVLS MPINDQFRKA VSN
Length:193
Mass (Da):22,014
Last modified:February 1, 1994 - v1
Checksum:iA300D104637AF801
GO

Sequence cautioni

The sequence AAW87994.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96844 Genomic DNA. Translation: AAA27543.1.
CP000021 Genomic DNA. Translation: AAW87994.1. Different initiation.
RefSeqiWP_011263744.1. NC_006841.2.
YP_206882.1. NC_006841.2.

Genome annotation databases

EnsemblBacteriaiAAW87994; AAW87994; VF_A0924.
GeneIDi3280753.
KEGGivfi:VF_A0924.
PATRICi20117845. VBIVibFis127983_3526.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96844 Genomic DNA. Translation: AAA27543.1.
CP000021 Genomic DNA. Translation: AAW87994.1. Different initiation.
RefSeqiWP_011263744.1. NC_006841.2.
YP_206882.1. NC_006841.2.

3D structure databases

ProteinModelPortaliP35328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi312309.VF_A0924.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW87994; AAW87994; VF_A0924.
GeneIDi3280753.
KEGGivfi:VF_A0924.
PATRICi20117845. VBIVibFis127983_3526.

Phylogenomic databases

eggNOGiENOG4105NNS. Bacteria.
COG3916. LUCA.
HOGENOMiHOG000207772.
KOiK13060.
OrthoDBiEOG60GRTV.

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-3681-MONOMER.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR018311. Autoind_synth_CS.
IPR001690. Autoind_synthase.
[Graphical view]
PfamiPF00765. Autoind_synth. 1 hit.
[Graphical view]
PRINTSiPR01549. AUTOINDCRSYN.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS00949. AUTOINDUCER_SYNTH_1. 1 hit.
PS51187. AUTOINDUCER_SYNTH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of luxR and luxI, the luminescence regulatory genes from the squid light organ symbiont Vibrio fischeri ES114."
    Gray K.M., Greenberg E.P.
    Mol. Mar. Biol. Biotechnol. 1:414-419(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiLUXJ_VIBF1
AccessioniPrimary (citable) accession number: P35328
Secondary accession number(s): Q5DZ02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: July 6, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.