P35321 (SPR1A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cornifin-A

Alternative name(s):
19 kDa pancornulin
SPRK
Small proline-rich protein IA
Short name=SPR-IA

Gene names

Name:

SPRR1A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.
Subcellular location	Cytoplasm.
Induction	During squamous differentiation of epidermal keratinocytes.
Sequence similarities	Belongs to the cornifin (SPRR) family.

Ontologies

Keywords
Biological process	Keratinization
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	Repeat
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	keratinization Inferred from electronic annotation. Source: UniProtKB-KW keratinocyte differentiation Inferred from direct assay PubMed 10908733. Source: UniProtKB peptide cross-linking Inferred from direct assay PubMed 10908733. Source: UniProtKB
Cellular_component	cornified envelope Inferred from direct assay PubMed 10908733. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein binding, bridging Inferred from direct assay PubMed 10908733. Source: UniProtKB structural molecule activity Inferred from direct assay PubMed 10908733. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 89	89	Cornifin-A		PRO_0000149995
Regions
	Repeat	3 – 14	12	1
	Repeat	18 – 29	12	2
	Repeat	31 – 38	8	1
	Repeat	39 – 46	8	2
	Repeat	47 – 54	8	3
	Repeat	55 – 62	8	4
	Repeat	63 – 70	8	5
	Repeat	71 – 78	8	6
	Region	3 – 29	27	2 X 12 AA approximate repeats
	Region	31 – 78	48	6 X 8 AA approximate tandem repeats
Natural variations
	Natural variant	42	1	H → Q. Ref.1 Ref.3 Ref.6 Corresponds to variant rs1611762 [ dbSNP | Ensembl ].		VAR_021097
	Natural variant	61	1	V → I. Ref.1 Ref.3 Ref.6 Corresponds to variant rs1611764 [ dbSNP | Ensembl ].		VAR_021098
Experimental info
	Sequence conflict	53	1	V → I AA sequence Ref.7
	Sequence conflict	65	1	Missing AA sequence Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P35321 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: C79E79931794D3D4	FASTA	89	9,877
	10 20 30 40 50 60 MNSQQQKQPC TPPPQPQQQQ VKQPCQPPPQ EPCIPKTKEP CHPKVPEPCH PKVPEPCQPK 70 80 VPEPCQPKVP EPCPSTVTPA PAQQKTKQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and evolution of the SPRR family of keratinocyte differentiation markers encoding small proline-rich proteins." Gibbs S., Fijneman R., Wiegant J., Geurts van Kessel A., van de Putte P., Backendorf C. Genomics 16:630-637(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-42 AND ILE-61. Tissue: Skin.
[2]	"Differential expression of protease inhibitor and small proline-rich protein genes between normal human oral tissue and odontogenic keratocysts." Robinson P.A., Marley J.J., High A.S., Hume W.J. Arch. Oral Biol. 39:251-259(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	NIEHS SNPs program Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLN-42 AND ILE-61.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS GLN-42 AND ILE-61.
[7]	"The pancornulins: a group of small proline rich-related cornified envelope precursors with bifunctional capabilities in isopeptide bond formation." Greco M.A., Lorand L., Lane W.S., Baden H.P., Parameswaran K.N.P., Kvedar J.C. J. Invest. Dermatol. 104:204-210(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 8-31 AND 53-85. Tissue: Keratinocyte.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05187 Genomic DNA. Translation: AAC26838.1. AY755659 Genomic DNA. Translation: AAU88143.1. AL356867 Genomic DNA. Translation: CAI19801.1. CH471121 Genomic DNA. Translation: EAW53355.1. CH471121 Genomic DNA. Translation: EAW53356.1. BC105081 mRNA. Translation: AAI05082.1. BC105083 mRNA. Translation: AAI05084.1.
IPI	IPI00017987.
PIR	I54187.
RefSeq	NP_001186757.1. NM_001199828.1. NP_005978.2. NM_005987.3.
UniGene	Hs.46320.
3D structure databases
ProteinModelPortal	P35321.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35321. 1 interaction.
STRING	9606.ENSP00000307340.
PTM databases
PhosphoSite	P35321.
Polymorphism databases
DMDM	215273890.
2D gel databases
UCD-2DPAGE	P35321.
Proteomic databases
PaxDb	P35321.
PRIDE	P35321.
Protocols and materials databases
DNASU	6698.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307122; ENSP00000307340; ENSG00000169474. ENST00000368762; ENSP00000357751; ENSG00000169474.
GeneID	6698.
KEGG	hsa:6698.
UCSC	uc001faw.3. human.
Organism-specific databases
CTD	6698.
GeneCards	GC01P152956.
H-InvDB	HIX0028873.
HGNC	HGNC:11259. SPRR1A.
HPA	CAB011237.
MIM	182265. gene.
neXtProt	NX_P35321.
PharmGKB	PA36088.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39062.
HOGENOM	HOG000059526.
HOVERGEN	HBG079213.
InParanoid	P35321.
OMA	CHPKEPC.
OrthoDB	EOG408N9R.
PhylomeDB	P35321.
Gene expression databases
Bgee	P35321.
CleanEx	HS_SPRR1A.
Genevestigator	P35321.
GermOnline	ENSG00000169474. Homo sapiens.
Family and domain databases
InterPro	IPR003302. Cornifin. IPR026075. SPRR/LCE. [Graphical view]
PANTHER	PTHR23263. PTHR23263. 1 hit.
Pfam	PF02389. Cornifin. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SPRR1A. human.
GenomeRNAi	6698.
NextBio	26115.
SOURCE	Search...

Entry information

Entry name

SPR1A_HUMAN

Accession

Primary (citable) accession number: P35321
Secondary accession number(s): B1AN47 , D3DV31, Q2M303, Q9UDG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 25, 2008
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.