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P35317 (AT1A_HYDVU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/potassium-transporting ATPase subunit alpha

Short name=Na(+)/K(+) ATPase alpha subunit
EC=3.6.3.9
Alternative name(s):
Sodium pump subunit alpha
OrganismHydra vulgaris (Hydra) (Hydra attenuata)
Taxonomic identifier6087 [NCBI]
Taxonomic lineageEukaryotaMetazoaCnidariaHydrozoaHydroidaAnthomedusaeHydridaeHydra

Protein attributes

Sequence length1031 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activity

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Enzyme regulation

This alpha subunit is resistant to ouabain.

Subunit structure

Composed of three subunits: alpha (catalytic), beta and gamma.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10311031Sodium/potassium-transporting ATPase subunit alpha
PRO_0000046308

Regions

Transmembrane102 – 12322Helical; By similarity
Transmembrane136 – 15520Helical; By similarity
Transmembrane297 – 31923Helical; By similarity
Transmembrane326 – 35429Helical; By similarity
Transmembrane795 – 81824Helical; By similarity
Transmembrane857 – 88226Helical; By similarity
Transmembrane924 – 94421Helical; By similarity
Transmembrane961 – 98626Helical; By similarity

Sites

Active site38214-aspartylphosphate intermediate Probable
Metal binding7251Magnesium By similarity
Metal binding7291Magnesium By similarity
Binding site5121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P35317 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: D2F4A07B811B356F

FASTA1,031114,161
        10         20         30         40         50         60 
MADPGDLESR GKADSYSVAE KKSAPKKISK KNANKAKLED LKKELEMTEH SMKLESLLSM 

        70         80         90        100        110        120 
YETSLEKGLS ENIVARNLER DGLNALTPPK QTPEWVKFCK QMFGGFSMLL WIGAILCFFA 

       130        140        150        160        170        180 
FGIRAVRDTN PNMDELYLGI VLSVVVIITG CFSYYQESKS SKIMESFKKM IPQEALVLRD 

       190        200        210        220        230        240 
GKKITINAEQ CVVGDVVFVK FGDRIPADIR IVECKGLKVD NSSLTGESEP QSRAVDFTHE 

       250        260        270        280        290        300 
NPIETKNLAF FSTNAVEGTA TGIVVRIGDN TVMGRIANLA SGLGSGKTPI ALEIEHFIHI 

       310        320        330        340        350        360 
VTGVAVFLGV SFLIISLAMG YHWLEAIIFL IGIIVANVPE GLLATVTVCL TLTAKKMAKK 

       370        380        390        400        410        420 
NCLVKHLEAV ETLGSTSVIC SDKTGTLTQN RMTVAHMWFD KMIVEADTTE DQSGIAHDKG 

       430        440        450        460        470        480 
SLTWKSLAKV AALCSRAEFK PNQNDVAVLR KECTGDASET AILKFVELSV GNVMDIRAKN 

       490        500        510        520        530        540 
KKVTEIPFNS TNKYQVSVHE QENSSGYLLV MKGAPEKVLE RCSTILINGE EQPLKDDVIE 

       550        560        570        580        590        600 
IYNKAYDELG GLGERVLGFC HYYLPVDQYP KGFLFKTEEE QNFPLEGLCF LGLLSMIDPP 

       610        620        630        640        650        660 
RAAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKGVGIIS EGNECEEDIA LRLNIPLEDL 

       670        680        690        700        710        720 
SEDQKKSAKA CVIHGAKLKD IKNEELDKIL CDHTEIVFAR TSPQQKLIIV EGCQRQGAIV 

       730        740        750        760        770        780 
AVTGDGVNDS PALKKADIGV AMGIAGSDVS KQAADMILLD DNFASIVTGV EEGRLIFDNL 

       790        800        810        820        830        840 
KKSIVYTLTS NIPEISPFLM FILFGIPLPL GTITILCIDL GTDMVPAISL AYEKAESDIM 

       850        860        870        880        890        900 
KRHPRNPIRD KLVNERLISL AYGQIGMMQA TAGFFTYFII LAENGFLPSY LFGLRSQWDD 

       910        920        930        940        950        960 
MSNNNLLDSF GSEWTYFQRK EIELTCQTAF FTTIVVVQWA DLIISKTRRL SLFQQGMTNW 

       970        980        990       1000       1010       1020 
FLNFGLFFET ALAAFLQYTP GVNTGLRLRP MNFTWWLPGL PFSLLIFVYD EIRRYLLRKN 

      1030 
PGGWVEKETY Y 

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References

[1]"Molecular cloning and characterization of Na,K-ATPase from Hydra vulgaris: implications for enzyme evolution and ouabain sensitivity."
Canfield V.A., Xu K.Y., D'Aquila T., Shyjan A.W., Levenson R.
New Biol. 4:339-348(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M75140 mRNA. Translation: AAA29207.1.

3D structure databases

ProteinModelPortalP35317.
SMRP35317. Positions 38-1031.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP35317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAT1A_HYDVU
AccessionPrimary (citable) accession number: P35317
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families