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P35317

- AT1A_HYDVU

UniProt

P35317 - AT1A_HYDVU

Protein

Sodium/potassium-transporting ATPase subunit alpha

Gene
N/A
Organism
Hydra vulgaris (Hydra) (Hydra attenuata)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Enzyme regulationi

    This alpha subunit is resistant to ouabain.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei382 – 38214-aspartylphosphate intermediateCurated
    Binding sitei512 – 5121ATPBy similarity
    Metal bindingi725 – 7251MagnesiumBy similarity
    Metal bindingi729 – 7291MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha subunit
    Alternative name(s):
    Sodium pump subunit alpha
    OrganismiHydra vulgaris (Hydra) (Hydra attenuata)
    Taxonomic identifieri6087 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidaAnthomedusaeHydridaeHydra

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10311031Sodium/potassium-transporting ATPase subunit alphaPRO_0000046308Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP35317.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.

    Structurei

    3D structure databases

    ProteinModelPortaliP35317.
    SMRiP35317. Positions 38-1031.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei102 – 12322HelicalBy similarityAdd
    BLAST
    Transmembranei136 – 15520HelicalBy similarityAdd
    BLAST
    Transmembranei297 – 31923HelicalBy similarityAdd
    BLAST
    Transmembranei326 – 35429HelicalBy similarityAdd
    BLAST
    Transmembranei795 – 81824HelicalBy similarityAdd
    BLAST
    Transmembranei857 – 88226HelicalBy similarityAdd
    BLAST
    Transmembranei924 – 94421HelicalBy similarityAdd
    BLAST
    Transmembranei961 – 98626HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35317-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPGDLESR GKADSYSVAE KKSAPKKISK KNANKAKLED LKKELEMTEH     50
    SMKLESLLSM YETSLEKGLS ENIVARNLER DGLNALTPPK QTPEWVKFCK 100
    QMFGGFSMLL WIGAILCFFA FGIRAVRDTN PNMDELYLGI VLSVVVIITG 150
    CFSYYQESKS SKIMESFKKM IPQEALVLRD GKKITINAEQ CVVGDVVFVK 200
    FGDRIPADIR IVECKGLKVD NSSLTGESEP QSRAVDFTHE NPIETKNLAF 250
    FSTNAVEGTA TGIVVRIGDN TVMGRIANLA SGLGSGKTPI ALEIEHFIHI 300
    VTGVAVFLGV SFLIISLAMG YHWLEAIIFL IGIIVANVPE GLLATVTVCL 350
    TLTAKKMAKK NCLVKHLEAV ETLGSTSVIC SDKTGTLTQN RMTVAHMWFD 400
    KMIVEADTTE DQSGIAHDKG SLTWKSLAKV AALCSRAEFK PNQNDVAVLR 450
    KECTGDASET AILKFVELSV GNVMDIRAKN KKVTEIPFNS TNKYQVSVHE 500
    QENSSGYLLV MKGAPEKVLE RCSTILINGE EQPLKDDVIE IYNKAYDELG 550
    GLGERVLGFC HYYLPVDQYP KGFLFKTEEE QNFPLEGLCF LGLLSMIDPP 600
    RAAVPDAVSK CRSAGIKVIM VTGDHPITAK AIAKGVGIIS EGNECEEDIA 650
    LRLNIPLEDL SEDQKKSAKA CVIHGAKLKD IKNEELDKIL CDHTEIVFAR 700
    TSPQQKLIIV EGCQRQGAIV AVTGDGVNDS PALKKADIGV AMGIAGSDVS 750
    KQAADMILLD DNFASIVTGV EEGRLIFDNL KKSIVYTLTS NIPEISPFLM 800
    FILFGIPLPL GTITILCIDL GTDMVPAISL AYEKAESDIM KRHPRNPIRD 850
    KLVNERLISL AYGQIGMMQA TAGFFTYFII LAENGFLPSY LFGLRSQWDD 900
    MSNNNLLDSF GSEWTYFQRK EIELTCQTAF FTTIVVVQWA DLIISKTRRL 950
    SLFQQGMTNW FLNFGLFFET ALAAFLQYTP GVNTGLRLRP MNFTWWLPGL 1000
    PFSLLIFVYD EIRRYLLRKN PGGWVEKETY Y 1031
    Length:1,031
    Mass (Da):114,161
    Last modified:February 1, 1994 - v1
    Checksum:iD2F4A07B811B356F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M75140 mRNA. Translation: AAA29207.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M75140 mRNA. Translation: AAA29207.1 .

    3D structure databases

    ProteinModelPortali P35317.
    SMRi P35317. Positions 38-1031.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P35317.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of Na,K-ATPase from Hydra vulgaris: implications for enzyme evolution and ouabain sensitivity."
      Canfield V.A., Xu K.Y., D'Aquila T., Shyjan A.W., Levenson R.
      New Biol. 4:339-348(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiAT1A_HYDVU
    AccessioniPrimary (citable) accession number: P35317
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3