Reviewed, UniProtKB/Swiss-Prot P35316 (ATC_ARTSF)

Names and origin

Protein names	Recommended name:     Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type     EC=3.6.3.8 Alternative name(s):     Calcium pump
Organism	Artemia sanfranciscana (Brine shrimp) (Artemia franciscana)
Taxonomic identifier	6661 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Crustacea › Branchiopoda › Anostraca › Artemiidae › Artemia

Protein attributes

Sequence length	1003 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.
Catalytic activity	ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).
Subcellular location	Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Developmental stage	Isoform 2 (long form) is expressed only in early stages of embryonic development (cysts), while isoform 1 (short form) is also found in later embryonic stages and adults.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane Sarcoplasmic reticulum
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Ligand	ATP-binding Calcium Magnesium Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell sarcoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P35316-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P35316-2)

The sequence of this isoform differs from the canonical sequence as follows:
     998-1003: EFSFIK → GMPLSSYFVDAWGLVLAWALFFGVIFYSPL

Note: Presents an extension, a potential transmembrane domain, which may have an important functional role.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1003	1003	Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type		PRO_0000046226
Regions
	Topological domain	1 – 59	59	Cytoplasmic Potential
	Transmembrane	60 – 78	19	Potential
	Topological domain	79 – 89	11	Extracellular Potential
	Transmembrane	90 – 110	21	Potential
	Topological domain	111 – 262	152	Cytoplasmic Potential
	Transmembrane	263 – 282	20	Potential
	Topological domain	283 – 300	18	Extracellular Potential
	Transmembrane	301 – 318	18	Potential
	Topological domain	319 – 775	457	Cytoplasmic Potential
	Transmembrane	776 – 799	24	Potential
	Topological domain	800 – 840	41	Extracellular Potential
	Transmembrane	841 – 863	23	Potential
	Topological domain	864 – 898	35	Cytoplasmic Potential
	Transmembrane	899 – 917	19	Potential
	Topological domain	918 – 934	17	Extracellular Potential
	Transmembrane	935 – 954	20	Potential
	Topological domain	955 – 1003	49	Cytoplasmic Potential
Sites
	Active site	354	1	4-aspartylphosphate intermediate By similarity
	Binding site	519	1	ATP By similarity
Natural variations
	Alternative sequence	998 – 1003	6	EFSFIK → GMPLSSYFVDAWGLVLAWAL FFGVIFYSPL in isoform 2.		VSP_000412
Experimental info
	Sequence conflict	481	1	T → A in CAA51262. Ref.2
	Sequence conflict	793	1	P → Q in CAA51262. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 908A036A19FAF03E	FASTA	1,003	110,344
	10 20 30 40 50 60 MEDAHAKKWE EVVDYFGVDP ERGLALEQVK KNQEKYGPNE LPAEEGKSLL TLILEQFDDL 70 80 90 100 110 120 LVKILLLAAI ISLVLALFEE HDDEAEQLTA YVEPFVILLI LIANAVVGVW QEKNAESAIE 130 140 150 160 170 180 ALKEYEPEMG KVIRADKTGI QKIKARDLVP GDIVEISVGD KIPADLRLIS ILSTTLRIDQ 190 200 210 220 230 240 SILTGESVSV IKHTDPVPDP RAVNQDKKNM LFSGTNVSAG KARGVVMGTG LNTAIGSIRT 250 260 270 280 290 300 QMFETEEMKT PLQQKLDEFG EQLSKVISVI CVAVWAINIG HFNDPAHGGS WIKGAIYYFK 310 320 330 340 350 360 IAVALAVAAI PEGLPAVITT CLALGTRRMA KKNAIVRSLP SVETLGCTSV ICSDKTGTLT 370 380 390 400 410 420 TNQMSVSRMF VFKDIPDDAA PELYQFELTG STYEPIGETF MQGQKINAAD YDAVKEITTI 430 440 450 460 470 480 CMMCNDSAID FNEYKQAFEK VGEATETALI VLGEKLNPYN LSKAGKDRRS AALVVREDMD 490 500 510 520 530 540 TRWKKEFTLE FSRDRKSMSS YCVPLKAGLL SNGPKMFVKG APEGVLDRCT HVRVGTKKVP 550 560 570 580 590 600 MTPAIMDKIL EVTRAYGTGR DTLRCLALAT IDDPMDPKDM DIIDSTKFVK YEQNCTFVGV 610 620 630 640 650 660 VGMLDPPRKE VLDAIERCRA AGIRVIVITG DNKATAEAIC RRIGVFGEDE NTEGMAYTGR 670 680 690 700 710 720 EFDDLSVEGQ RDAVARSRLF ARVEPFHKSK IVEYLQGMGE ISAMTGDGVN DAPALKKAEI 730 740 750 760 770 780 GIAMGSGTAV AKSAAEMVLA DDNFSTIVAA VEEGRAIYNN MKQFIRYLIS SNIGEVVSIF 790 800 810 820 830 840 LTAALGLPEA LIPVQLLWVN LVTDGLPATA LGFNPPDLDI MNKPPRRADE GLITGWLFFR 850 860 870 880 890 900 YMAIGTYVGA ATVGAAAHWF MMSPTGPGLN FYQLSHHLQC TPENEYFEGI DCEIFSDPHP 910 920 930 940 950 960 MTMALSVLVT IEMLNAINSL SENQSLLVMP PWSNIWLISA ICLSMTLHFV ILYVEILSTV 970 980 990 1000 FQICPLTLTE WIVVLKISFP VLLLDEVLKF VARKYTDEFS FIK
	Isoform 2. Checksum: ACE4E39FF5131D66	FASTA	1,027	112,942
	10 20 30 40 50 60 MEDAHAKKWE EVVDYFGVDP ERGLALEQVK KNQEKYGPNE LPAEEGKSLL TLILEQFDDL 70 80 90 100 110 120 LVKILLLAAI ISLVLALFEE HDDEAEQLTA YVEPFVILLI LIANAVVGVW QEKNAESAIE 130 140 150 160 170 180 ALKEYEPEMG KVIRADKTGI QKIKARDLVP GDIVEISVGD KIPADLRLIS ILSTTLRIDQ 190 200 210 220 230 240 SILTGESVSV IKHTDPVPDP RAVNQDKKNM LFSGTNVSAG KARGVVMGTG LNTAIGSIRT 250 260 270 280 290 300 QMFETEEMKT PLQQKLDEFG EQLSKVISVI CVAVWAINIG HFNDPAHGGS WIKGAIYYFK 310 320 330 340 350 360 IAVALAVAAI PEGLPAVITT CLALGTRRMA KKNAIVRSLP SVETLGCTSV ICSDKTGTLT 370 380 390 400 410 420 TNQMSVSRMF VFKDIPDDAA PELYQFELTG STYEPIGETF MQGQKINAAD YDAVKEITTI 430 440 450 460 470 480 CMMCNDSAID FNEYKQAFEK VGEATETALI VLGEKLNPYN LSKAGKDRRS AALVVREDMD 490 500 510 520 530 540 TRWKKEFTLE FSRDRKSMSS YCVPLKAGLL SNGPKMFVKG APEGVLDRCT HVRVGTKKVP 550 560 570 580 590 600 MTPAIMDKIL EVTRAYGTGR DTLRCLALAT IDDPMDPKDM DIIDSTKFVK YEQNCTFVGV 610 620 630 640 650 660 VGMLDPPRKE VLDAIERCRA AGIRVIVITG DNKATAEAIC RRIGVFGEDE NTEGMAYTGR 670 680 690 700 710 720 EFDDLSVEGQ RDAVARSRLF ARVEPFHKSK IVEYLQGMGE ISAMTGDGVN DAPALKKAEI 730 740 750 760 770 780 GIAMGSGTAV AKSAAEMVLA DDNFSTIVAA VEEGRAIYNN MKQFIRYLIS SNIGEVVSIF 790 800 810 820 830 840 LTAALGLPEA LIPVQLLWVN LVTDGLPATA LGFNPPDLDI MNKPPRRADE GLITGWLFFR 850 860 870 880 890 900 YMAIGTYVGA ATVGAAAHWF MMSPTGPGLN FYQLSHHLQC TPENEYFEGI DCEIFSDPHP 910 920 930 940 950 960 MTMALSVLVT IEMLNAINSL SENQSLLVMP PWSNIWLISA ICLSMTLHFV ILYVEILSTV 970 980 990 1000 1010 1020 FQICPLTLTE WIVVLKISFP VLLLDEVLKF VARKYTDGMP LSSYFVDAWG LVLAWALFFG VIFYSPL

References

[1]	"Complementary DNA cloning of a protein highly homologous to mammalian sarcoplasmic reticulum Ca-ATPase from the crustacean Artemia." Palmero I., Sastre L. J. Mol. Biol. 210:737-748(1989) [PubMed: 2533270] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Similar alternative splicing events generate two sarcoplasmic or endoplasmic reticulum Ca-ATPase isoforms in the crustacean Artemia franciscana and in vertebrates." Escalante R., Sastre L. J. Biol. Chem. 268:14090-14095(1993) [PubMed: 8314776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 354-1003. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X51674 mRNA. Translation: CAA35980.1. X72713 Genomic DNA. Translation: CAA51262.1.
PIR	S07526. S32230.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR005782. ATPase_P-typ_Ca-transp. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01116. ATPase-IIA1_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATC_ARTSF

Accession

Primary (citable) accession number: P35316

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)