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P35316 (ATC_ARTSF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type
EC=3.6.3.8
Alternative name(s):
Calcium pump
OrganismArtemia franciscana (Brine shrimp) (Artemia sanfranciscana)
Taxonomic identifier6661 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Crustacea › Branchiopoda › Anostraca › Artemiidae › Artemia

Protein attributes

Sequence length1003 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.

Catalytic activity

ATP + H2O + Ca2+[side 1] = ADP + phosphate + Ca2+[side 2].

Subcellular location

Sarcoplasmic reticulum membrane; Multi-pass membrane protein.

Developmental stage

Isoform 2 (long form) is expressed only in early stages of embryonic development (cysts), while isoform 1 (short form) is also found in later embryonic stages and adults.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P35316-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P35316-2)

The sequence of this isoform differs from the canonical sequence as follows:
     998-1003: EFSFIK → GMPLSSYFVDAWGLVLAWALFFGVIFYSPL
Note: Presents an extension, a potential transmembrane domain, which may have an important functional role.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10031003Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type
PRO_0000046226

Regions

Topological domain1 – 5959Cytoplasmic Potential
Transmembrane60 – 7819Helical; Potential
Topological domain79 – 8911Extracellular Potential
Transmembrane90 – 11021Helical; Potential
Topological domain111 – 262152Cytoplasmic Potential
Transmembrane263 – 28220Helical; Potential
Topological domain283 – 30018Extracellular Potential
Transmembrane301 – 31818Helical; Potential
Topological domain319 – 775457Cytoplasmic Potential
Transmembrane776 – 79924Helical; Potential
Topological domain800 – 84041Extracellular Potential
Transmembrane841 – 86323Helical; Potential
Topological domain864 – 89835Cytoplasmic Potential
Transmembrane899 – 91719Helical; Potential
Topological domain918 – 93417Extracellular Potential
Transmembrane935 – 95420Helical; Potential
Topological domain955 – 100349Cytoplasmic Potential

Sites

Active site35414-aspartylphosphate intermediate By similarity
Binding site5191ATP By similarity

Natural variations

Alternative sequence998 – 10036EFSFIK → GMPLSSYFVDAWGLVLAWAL FFGVIFYSPL in isoform 2.
VSP_000412

Experimental info

Sequence conflict4811T → A in CAA51262. Ref.2
Sequence conflict7931P → Q in CAA51262. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 908A036A19FAF03E

FASTA1,003110,344
        10         20         30         40         50         60 
MEDAHAKKWE EVVDYFGVDP ERGLALEQVK KNQEKYGPNE LPAEEGKSLL TLILEQFDDL 

        70         80         90        100        110        120 
LVKILLLAAI ISLVLALFEE HDDEAEQLTA YVEPFVILLI LIANAVVGVW QEKNAESAIE 

       130        140        150        160        170        180 
ALKEYEPEMG KVIRADKTGI QKIKARDLVP GDIVEISVGD KIPADLRLIS ILSTTLRIDQ 

       190        200        210        220        230        240 
SILTGESVSV IKHTDPVPDP RAVNQDKKNM LFSGTNVSAG KARGVVMGTG LNTAIGSIRT 

       250        260        270        280        290        300 
QMFETEEMKT PLQQKLDEFG EQLSKVISVI CVAVWAINIG HFNDPAHGGS WIKGAIYYFK 

       310        320        330        340        350        360 
IAVALAVAAI PEGLPAVITT CLALGTRRMA KKNAIVRSLP SVETLGCTSV ICSDKTGTLT 

       370        380        390        400        410        420 
TNQMSVSRMF VFKDIPDDAA PELYQFELTG STYEPIGETF MQGQKINAAD YDAVKEITTI 

       430        440        450        460        470        480 
CMMCNDSAID FNEYKQAFEK VGEATETALI VLGEKLNPYN LSKAGKDRRS AALVVREDMD 

       490        500        510        520        530        540 
TRWKKEFTLE FSRDRKSMSS YCVPLKAGLL SNGPKMFVKG APEGVLDRCT HVRVGTKKVP 

       550        560        570        580        590        600 
MTPAIMDKIL EVTRAYGTGR DTLRCLALAT IDDPMDPKDM DIIDSTKFVK YEQNCTFVGV 

       610        620        630        640        650        660 
VGMLDPPRKE VLDAIERCRA AGIRVIVITG DNKATAEAIC RRIGVFGEDE NTEGMAYTGR 

       670        680        690        700        710        720 
EFDDLSVEGQ RDAVARSRLF ARVEPFHKSK IVEYLQGMGE ISAMTGDGVN DAPALKKAEI 

       730        740        750        760        770        780 
GIAMGSGTAV AKSAAEMVLA DDNFSTIVAA VEEGRAIYNN MKQFIRYLIS SNIGEVVSIF 

       790        800        810        820        830        840 
LTAALGLPEA LIPVQLLWVN LVTDGLPATA LGFNPPDLDI MNKPPRRADE GLITGWLFFR 

       850        860        870        880        890        900 
YMAIGTYVGA ATVGAAAHWF MMSPTGPGLN FYQLSHHLQC TPENEYFEGI DCEIFSDPHP 

       910        920        930        940        950        960 
MTMALSVLVT IEMLNAINSL SENQSLLVMP PWSNIWLISA ICLSMTLHFV ILYVEILSTV 

       970        980        990       1000 
FQICPLTLTE WIVVLKISFP VLLLDEVLKF VARKYTDEFS FIK

« Hide

Isoform 2 [UniParc].

Checksum: ACE4E39FF5131D66
Show »

FASTA1,027112,942

References

[1]"Complementary DNA cloning of a protein highly homologous to mammalian sarcoplasmic reticulum Ca-ATPase from the crustacean Artemia."
Palmero I., Sastre L.
J. Mol. Biol. 210:737-748(1989) [PubMed: 2533270] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Similar alternative splicing events generate two sarcoplasmic or endoplasmic reticulum Ca-ATPase isoforms in the crustacean Artemia franciscana and in vertebrates."
Escalante R., Sastre L.
J. Biol. Chem. 268:14090-14095(1993) [PubMed: 8314776] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 354-1003.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X51674 mRNA. Translation: CAA35980.1.
X72713 Genomic DNA. Translation: CAA51262.1.
PIRS07526.
S32230.

3D structure databases

ProteinModelPortalP35316.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR023306. ATPase_cation_domN.
IPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR005782. ATPase_P-typ_Ca-transp.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 2 hits.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF81660. ATPase_cation_domN. 1 hit.
SSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameATC_ARTSF
AccessionPrimary (citable) accession number: P35316
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

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