P35295 (RAB20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-20

Gene names

Name:

Rab20

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium. Plays a role in the fusion of phagosomes with lysosomes By similarity.
Subcellular location	Golgi apparatus By similarity. Cytoplasmic vesicle › phagosome By similarity. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Highly enriched on apical endocytic structures in polarized epithelial cells of kidney proximal tubules.
Tissue specificity	Present in a variety of tissues, but not in brain.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phagosome acidification Inferred from sequence or structural similarity. Source: UniProtKB phagosome-lysosome fusion Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Ras-related protein Rab-20		PRO_0000121203
Regions
	Nucleotide binding	12 – 19	8	GTP By similarity
	Nucleotide binding	55 – 59	5	GTP By similarity
	Nucleotide binding	113 – 116	4	GTP By similarity
	Motif	33 – 41	9	Effector region By similarity
Amino acid modifications
	Lipidation	231	1	S-geranylgeranyl cysteine By similarity
	Lipidation	232	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35295 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 3A2E70A18D5280A9	FASTA	233	25,989
	10 20 30 40 50 60 MRKPDGKIVL LGDMNVGKTS LLQRYMERRF PDTVSTVGGA FYLKQWRSFN ISIWDTAGRE 70 80 90 100 110 120 QFHGLGSLYC RGAAAIILTY DVNHPQSLFE LEDRFLGLTE TANNDCLFAI VGNKVDLTSE 130 140 150 160 170 180 RDTEGGEKEG PASGKVGSCV STKVPKQVQP EDAVALYKKI LKYKMLDERE MPAAEQMCFE 190 200 210 220 230 TSAKTGYNVD LLFETLFDLV VPMIMRQRAE ESDQTVDIAS CKTPKQTRSG CCA

References

[1]	"Cloning and subcellular localization of novel rab proteins reveals polarized and cell type-specific expression." Luetcke A., Parton R.G., Murphy C., Olkkonen V.M., Dupree P., Valencia A., Simons K., Zerial M. J. Cell Sci. 107:3437-3448(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach." Chavrier P., Simons K., Zerial M. Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-60. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80332 mRNA. Translation: CAA56582.1. M79310 mRNA. Translation: AAK14834.1.
IPI	IPI00116777.
PIR	S46950. I48728.
RefSeq	NP_035357.1. NM_011227.1.
UniGene	Mm.37632. Mm.390014.
3D structure databases
ProteinModelPortal	P35295.
SMR	P35295. Positions 7-193.
ModBase	Search...
PTM databases
PhosphoSite	P35295.
Proteomic databases
PRIDE	P35295.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033900; ENSMUSP00000033900; ENSMUSG00000031504.
GeneID	19332.
KEGG	mmu:19332.
Organism-specific databases
CTD	55647.
MGI	MGI:102789. Rab20.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG097927.
InParanoid	P35295.
KO	K07911.
OMA	MRKPDGK.
OrthoDB	EOG4F4SC1.
Gene expression databases
ArrayExpress	P35295.
Bgee	P35295.
CleanEx	MM_RAB20.
Genevestigator	P35295.
GermOnline	ENSMUSG00000031504. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RAB20. mouse.
NextBio	296315.
SOURCE	Search...

Entry information

Entry name

RAB20_MOUSE

Accession

Primary (citable) accession number: P35295

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program