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P35292

- RAB17_MOUSE

UniProt

P35292 - RAB17_MOUSE

Protein

Ras-related protein Rab-17

Gene

Rab17

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. Mainly observed in epithelial cells, transcytosis mediates for instance, the transcellular transport of immunoglobulins from the basolateral surface to the apical surface. Most probably controls membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis. Required for melanosome transport and release from melanocytes, it also regulates dendrite and dendritic spine development. May also play a role in cell migration.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 338GTPBy similarity
    Nucleotide bindingi73 – 775GTPBy similarity
    Nucleotide bindingi132 – 1354GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. endocytic recycling Source: UniProtKB
    2. establishment of melanosome localization Source: UniProtKB
    3. filopodium assembly Source: UniProtKB
    4. immunoglobulin transcytosis in epithelial cells mediated by polymeric immunoglobulin receptor Source: UniProtKB
    5. melanosome transport Source: UniProtKB
    6. protein transport Source: UniProtKB-KW
    7. regulation of dendrite development Source: UniProtKB
    8. regulation of filopodium assembly Source: UniProtKB
    9. regulation of synapse assembly Source: UniProtKB
    10. small GTPase mediated signal transduction Source: InterPro
    11. transcytosis Source: UniProtKB

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-17
    Gene namesi
    Name:Rab17
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:104640. Rab17.

    Subcellular locationi

    Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Cell projectiondendrite
    Note: According to a report the protein is localized at the basolateral and apical plasma membrane of kidney epithelial cells (PubMed:8486736). It was later shown to localize to the apical recycling endosome in epithelial cells (PubMed:21291502). In neurons, localizes to the cell body and dendritic shaft and spine.2 Publications

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basolateral plasma membrane Source: UniProtKB
    3. dendrite Source: UniProtKB
    4. melanosome Source: UniProtKB
    5. neuronal cell body Source: UniProtKB
    6. recycling endosome Source: UniProtKB
    7. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi77 – 771Q → L: Probable constitutively active mutant unable to hydrolyze GTP; increases dendrite number and length. 2 Publications
    Mutagenesisi211 – 2122CC → AA: Loss of association with membranes and redistribution to the cytosol. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Ras-related protein Rab-17PRO_0000121192Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi211 – 2111S-geranylgeranyl cysteine1 Publication
    Lipidationi212 – 2121S-geranylgeranyl cysteine1 Publication

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiP35292.
    PaxDbiP35292.
    PRIDEiP35292.

    PTM databases

    PhosphoSiteiP35292.

    Expressioni

    Tissue specificityi

    Expressed in kidney, liver, and intestine mainly by epithelial cells. Expressed in hippocampus (at protein level).2 Publications

    Developmental stagei

    Expression starts at E5 and gradually increases from P5 to adulthood (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP35292.
    BgeeiP35292.
    CleanExiMM_RAB17.
    GenevestigatoriP35292.

    Interactioni

    Protein-protein interaction databases

    BioGridi202535. 2 interactions.
    MINTiMINT-1340501.

    Structurei

    3D structure databases

    ProteinModelPortaliP35292.
    SMRiP35292. Positions 21-207.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 559Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP35292.
    KOiK07909.
    OMAiSVCHLYY.
    OrthoDBiEOG7J9VQP.
    PhylomeDBiP35292.
    TreeFamiTF300199.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35292-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQAAGLPQA STASGQPYVS KLVLLGSSSV GKTSLALRYM KQDFSNVLPT    50
    VGCAFFTKVL DLGSSSLKLE IWDTAGQEKY QSVCHLYFRG ANAALLVYDI 100
    TRKDSFHKAQ QWLEDLEKEF QPGEVVVMLV GNKTDLGEER EVTFQEGKEF 150
    AESKSLLFME TSAKLNYQVS EIFNTVAQEL LQRAGDTGSS RPQEGEAVAL 200
    NQEPPIRQRQ CCAR 214
    Length:214
    Mass (Da):23,640
    Last modified:February 1, 1994 - v1
    Checksum:i11052D3EC730EFAB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191V → L in BAC35842. (PubMed:15489334)Curated
    Sequence conflicti57 – 571T → K in BAB26452. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70804 mRNA. Translation: CAA50071.1.
    AK009707 mRNA. Translation: BAB26452.1.
    AK075591 mRNA. Translation: BAC35842.1.
    BC013170 mRNA. Translation: AAH13170.1.
    BC051071 mRNA. Translation: AAH51071.1.
    M79307 mRNA. Translation: AAK14831.1.
    CCDSiCCDS15156.1.
    PIRiA46434.
    RefSeqiNP_001153197.2. NM_001159725.2.
    NP_033024.1. NM_008998.4.
    XP_006529326.1. XM_006529263.1.
    UniGeneiMm.279780.

    Genome annotation databases

    EnsembliENSMUST00000027529; ENSMUSP00000027529; ENSMUSG00000026304.
    ENSMUST00000131428; ENSMUSP00000122178; ENSMUSG00000026304.
    GeneIDi19329.
    KEGGimmu:19329.
    UCSCiuc007bzn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70804 mRNA. Translation: CAA50071.1 .
    AK009707 mRNA. Translation: BAB26452.1 .
    AK075591 mRNA. Translation: BAC35842.1 .
    BC013170 mRNA. Translation: AAH13170.1 .
    BC051071 mRNA. Translation: AAH51071.1 .
    M79307 mRNA. Translation: AAK14831.1 .
    CCDSi CCDS15156.1.
    PIRi A46434.
    RefSeqi NP_001153197.2. NM_001159725.2.
    NP_033024.1. NM_008998.4.
    XP_006529326.1. XM_006529263.1.
    UniGenei Mm.279780.

    3D structure databases

    ProteinModelPortali P35292.
    SMRi P35292. Positions 21-207.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202535. 2 interactions.
    MINTi MINT-1340501.

    PTM databases

    PhosphoSitei P35292.

    Proteomic databases

    MaxQBi P35292.
    PaxDbi P35292.
    PRIDEi P35292.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027529 ; ENSMUSP00000027529 ; ENSMUSG00000026304 .
    ENSMUST00000131428 ; ENSMUSP00000122178 ; ENSMUSG00000026304 .
    GeneIDi 19329.
    KEGGi mmu:19329.
    UCSCi uc007bzn.2. mouse.

    Organism-specific databases

    CTDi 64284.
    MGIi MGI:104640. Rab17.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P35292.
    KOi K07909.
    OMAi SVCHLYY.
    OrthoDBi EOG7J9VQP.
    PhylomeDBi P35292.
    TreeFami TF300199.

    Miscellaneous databases

    NextBioi 296301.
    PROi P35292.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35292.
    Bgeei P35292.
    CleanExi MM_RAB17.
    Genevestigatori P35292.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rab17, a novel small GTPase, is specific for epithelial cells and is induced during cell polarization."
      Luetcke A., Jansson S., Parton R.G., Chavrier P., Valencia A., Huber L.A., Lehtonen E., Zerial M.
      J. Cell Biol. 121:553-564(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney and Tongue.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.
    4. "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
      Chavrier P., Simons K., Zerial M.
      Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-78.
      Tissue: Kidney.
    5. "Rab17 localizes to recycling endosomes and regulates receptor-mediated transcytosis in epithelial cells."
      Hunziker W., Peters P.J.
      J. Biol. Chem. 273:15734-15741(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSCYTOSIS, SUBCELLULAR LOCATION, TOPOLOGY, ISOPRENYLATION AT CYS-211 AND CYS-212, MUTAGENESIS OF 211-CYS-CYS-212.
    6. "Rab17 regulates membrane trafficking through apical recycling endosomes in polarized epithelial cells."
      Zacchi P., Stenmark H., Parton R.G., Orioli D., Lim F., Giner A., Mellman I., Zerial M., Murphy C.
      J. Cell Biol. 140:1039-1053(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking."
      Beaumont K.A., Hamilton N.A., Moores M.T., Brown D.L., Ohbayashi N., Cairncross O., Cook A.L., Smith A.G., Misaki R., Fukuda M., Taguchi T., Sturm R.A., Stow J.L.
      Traffic 12:627-643(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MELANOSOME TRANSPORT, SUBCELLULAR LOCATION.
    8. "Small GTPase Rab17 regulates dendritic morphogenesis and postsynaptic development of hippocampal neurons."
      Mori Y., Matsui T., Furutani Y., Yoshihara Y., Fukuda M.
      J. Biol. Chem. 287:8963-8973(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DENDRITOGENESIS, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLN-77.

    Entry informationi

    Entry nameiRAB17_MOUSE
    AccessioniPrimary (citable) accession number: P35292
    Secondary accession number(s): Q921D2, Q9D723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3