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P35290

- RAB24_MOUSE

UniProt

P35290 - RAB24_MOUSE

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Protein

Ras-related protein Rab-24

Gene

Rab24

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in autophagy-related processes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 229GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi120 – 1234GTPBy similarity
Nucleotide bindingi154 – 1563GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. protein transport Source: UniProtKB-KW
  3. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-24
Alternative name(s):
Rab-16
Gene namesi
Name:Rab24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:105065. Rab24.

Subcellular locationi

Cytoplasmcytosol. Membrane; Lipid-anchor
Note: Only about 20% is recovered in the particulate fraction.

GO - Cellular componenti

  1. autophagic vacuole Source: MGI
  2. membrane Source: UniProtKB-KW
  3. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi22 – 221S → N: Predominantly in the GTP-bound state. No effect on subcellular location. 1 Publication
Mutagenesisi67 – 671S → Q: Increase in GTPase activity. No effect neither on prenylation, nor on subcellular location. 1 Publication
Mutagenesisi120 – 1201T → A or I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. 1 Publication
Mutagenesisi123 – 1231D → I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Disruptes the integrity of the nuclear envelope. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Ras-related protein Rab-24PRO_0000121214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity
Lipidationi201 – 2011S-geranylgeranyl cysteineBy similarity

Post-translational modificationi

Isoprenylation is inefficient compared to other Rab family members.

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

MaxQBiP35290.
PaxDbiP35290.
PRIDEiP35290.

PTM databases

PhosphoSiteiP35290.

Expressioni

Tissue specificityi

Widely expressed, with highest expression in brain.1 Publication

Gene expression databases

BgeeiP35290.
CleanExiMM_RAB24.
ExpressionAtlasiP35290. baseline and differential.
GenevestigatoriP35290.

Interactioni

Subunit structurei

Unlike other Rab family members, does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB.

Protein-protein interaction databases

IntActiP35290. 1 interaction.
MINTiMINT-7899035.

Structurei

3D structure databases

ProteinModelPortaliP35290.
SMRiP35290. Positions 9-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35290.
KOiK07912.
OMAiFWVKELQ.
OrthoDBiEOG7B8S58.
PhylomeDBiP35290.
TreeFamiTF300199.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35290-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGQRVDVKV VMLGKEYVGK TSLVERYVHD RFLVGPYQNT IGAAFVAKVM
60 70 80 90 100
CVGDRTVTLG IWDTAGSERY EAMSRIYYRG AKAAIVCYDL TDSSSFERAK
110 120 130 140 150
FWVKELRSLE EGCQIYLCGT KSDLLEEDRR RRRVDFHDVQ DYADNIKAQL
160 170 180 190 200
FETSSKTGQS VDELFQKVAE DYVSVAAFQV MTEDKGVDLS QKANPYFYSC

CHH
Length:203
Mass (Da):23,144
Last modified:October 1, 1996 - v2
Checksum:i872EA5E2E7B5B0F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671S → Q in AAK14830. (PubMed:1555775)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22819 mRNA. Translation: CAA80472.1.
BC019950 mRNA. Translation: AAH19950.1.
BC054466 mRNA. Translation: AAH54466.1.
BC055894 mRNA. Translation: AAH55894.1.
M79306 mRNA. Translation: AAK14830.1.
CCDSiCCDS26541.1.
PIRiJH0646.
S40235.
RefSeqiNP_033026.1. NM_009000.3.
UniGeneiMm.220923.

Genome annotation databases

EnsembliENSMUST00000035242; ENSMUSP00000046188; ENSMUSG00000034789.
GeneIDi19336.
KEGGimmu:19336.
UCSCiuc007qqk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22819 mRNA. Translation: CAA80472.1 .
BC019950 mRNA. Translation: AAH19950.1 .
BC054466 mRNA. Translation: AAH54466.1 .
BC055894 mRNA. Translation: AAH55894.1 .
M79306 mRNA. Translation: AAK14830.1 .
CCDSi CCDS26541.1.
PIRi JH0646.
S40235.
RefSeqi NP_033026.1. NM_009000.3.
UniGenei Mm.220923.

3D structure databases

ProteinModelPortali P35290.
SMRi P35290. Positions 9-170.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35290. 1 interaction.
MINTi MINT-7899035.

PTM databases

PhosphoSitei P35290.

Proteomic databases

MaxQBi P35290.
PaxDbi P35290.
PRIDEi P35290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035242 ; ENSMUSP00000046188 ; ENSMUSG00000034789 .
GeneIDi 19336.
KEGGi mmu:19336.
UCSCi uc007qqk.1. mouse.

Organism-specific databases

CTDi 53917.
MGIi MGI:105065. Rab24.

Phylogenomic databases

eggNOGi COG1100.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P35290.
KOi K07912.
OMAi FWVKELQ.
OrthoDBi EOG7B8S58.
PhylomeDBi P35290.
TreeFami TF300199.

Miscellaneous databases

NextBioi 296327.
PROi P35290.
SOURCEi Search...

Gene expression databases

Bgeei P35290.
CleanExi MM_RAB24.
ExpressionAtlasi P35290. baseline and differential.
Genevestigatori P35290.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily."
    Olkkonen V.M., Dupree P., Killisch I., Luetcke A., Simons K., Zerial M.
    J. Cell Sci. 106:1249-1261(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary gland.
  3. "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
    Chavrier P., Simons K., Zerial M.
    Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68.
    Tissue: Kidney.
  4. "Rab24 is an atypical member of the Rab GTPase family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells."
    Erdman R.A., Shellenberger K.E., Overmeyer J.H., Maltese W.A.
    J. Biol. Chem. 275:3848-3856(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INEFFICIENT ISOPRENYLATION, LACK OF INTERACTION WITH ARHGDIA AND ARHGDIB, MUTAGENESIS OF SER-67.
  5. "Mutant Rab24 GTPase is targeted to nuclear inclusions."
    Maltese W.A., Soule G., Gunning W., Calomeni E., Alexander B.
    BMC Cell Biol. 3:25-25(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-22; THR-120 AND ASP-123.

Entry informationi

Entry nameiRAB24_MOUSE
AccessioniPrimary (citable) accession number: P35290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The unusual Ser-67, instead of a conserved Gln in other family members, is the cause of low GTPase activity. As a result, the predominant nucleotide associated with the protein is GTP.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3