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P35290 (RAB24_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-24
Alternative name(s):
Rab-16
Gene names
Name:Rab24
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length203 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in autophagy-related processes.

Subunit structure

Unlike other Rab family members, does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB.

Subcellular location

Cytoplasmcytosol. Membrane; Lipid-anchor. Note: Only about 20% is recovered in the particulate fraction. Ref.4 Ref.5

Tissue specificity

Widely expressed, with highest expression in brain. Ref.1

Post-translational modification

Isoprenylation is inefficient compared to other Rab family members.

Miscellaneous

The unusual Ser-67, instead of a conserved Gln in other family members, is the cause of low GTPase activity. As a result, the predominant nucleotide associated with the protein is GTP.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 203203Ras-related protein Rab-24
PRO_0000121214

Regions

Nucleotide binding14 – 229GTP By similarity
Nucleotide binding63 – 675GTP By similarity
Nucleotide binding120 – 1234GTP By similarity
Nucleotide binding154 – 1563GTP By similarity
Motif37 – 459Effector region By similarity

Amino acid modifications

Lipidation2001S-geranylgeranyl cysteine By similarity
Lipidation2011S-geranylgeranyl cysteine By similarity

Experimental info

Mutagenesis221S → N: Predominantly in the GTP-bound state. No effect on subcellular location. Ref.5
Mutagenesis671S → Q: Increase in GTPase activity. No effect neither on prenylation, nor on subcellular location. Ref.4
Mutagenesis1201T → A or I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Ref.5
Mutagenesis1231D → I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Disruptes the integrity of the nuclear envelope. Ref.5
Sequence conflict671S → Q in AAK14830. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P35290 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 872EA5E2E7B5B0F0

FASTA20323,144
        10         20         30         40         50         60 
MSGQRVDVKV VMLGKEYVGK TSLVERYVHD RFLVGPYQNT IGAAFVAKVM CVGDRTVTLG 

        70         80         90        100        110        120 
IWDTAGSERY EAMSRIYYRG AKAAIVCYDL TDSSSFERAK FWVKELRSLE EGCQIYLCGT 

       130        140        150        160        170        180 
KSDLLEEDRR RRRVDFHDVQ DYADNIKAQL FETSSKTGQS VDELFQKVAE DYVSVAAFQV 

       190        200 
MTEDKGVDLS QKANPYFYSC CHH 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily."
Olkkonen V.M., Dupree P., Killisch I., Luetcke A., Simons K., Zerial M.
J. Cell Sci. 106:1249-1261(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon and Mammary gland.
[3]"The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
Chavrier P., Simons K., Zerial M.
Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68.
Tissue: Kidney.
[4]"Rab24 is an atypical member of the Rab GTPase family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells."
Erdman R.A., Shellenberger K.E., Overmeyer J.H., Maltese W.A.
J. Biol. Chem. 275:3848-3856(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INEFFICIENT ISOPRENYLATION, LACK OF INTERACTION WITH ARHGDIA AND ARHGDIB, MUTAGENESIS OF SER-67.
[5]"Mutant Rab24 GTPase is targeted to nuclear inclusions."
Maltese W.A., Soule G., Gunning W., Calomeni E., Alexander B.
BMC Cell Biol. 3:25-25(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-22; THR-120 AND ASP-123.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z22819 mRNA. Translation: CAA80472.1.
BC019950 mRNA. Translation: AAH19950.1.
BC054466 mRNA. Translation: AAH54466.1.
BC055894 mRNA. Translation: AAH55894.1.
M79306 mRNA. Translation: AAK14830.1.
PIRJH0646.
S40235.
RefSeqNP_033026.1. NM_009000.3.
UniGeneMm.220923.

3D structure databases

ProteinModelPortalP35290.
SMRP35290. Positions 2-201.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP35290. 1 interaction.
MINTMINT-7899035.

PTM databases

PhosphoSiteP35290.

Proteomic databases

PaxDbP35290.
PRIDEP35290.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035242; ENSMUSP00000046188; ENSMUSG00000034789.
GeneID19336.
KEGGmmu:19336.
UCSCuc007qqk.1. mouse.

Organism-specific databases

CTD53917.
MGIMGI:105065. Rab24.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP35290.
KOK07912.
OMAFWVKELQ.
OrthoDBEOG7B8S58.
PhylomeDBP35290.
TreeFamTF300199.

Gene expression databases

ArrayExpressP35290.
BgeeP35290.
CleanExMM_RAB24.
GenevestigatorP35290.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296327.
PROP35290.
SOURCESearch...

Entry information

Entry nameRAB24_MOUSE
AccessionPrimary (citable) accession number: P35290
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot