P35290 (RAB24_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-24

Alternative name(s):
Rab-16

Gene names

Name:

Rab24

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in autophagy-related processes.
Subunit structure	Unlike other Rab family members, does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB.
Subcellular location	Cytoplasm › cytosol. Membrane; Lipid-anchor. Note: Only about 20% is recovered in the particulate fraction. Ref.4 Ref.5
Tissue specificity	Widely expressed, with highest expression in brain. Ref.1
Post-translational modification	Isoprenylation is inefficient compared to other Rab family members.
Miscellaneous	The unusual Ser-67, instead of a conserved Gln in other family members, is the cause of low GTPase activity. As a result, the predominant nucleotide associated with the protein is GTP.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	autophagic vacuole Inferred from direct assay PubMed 19368996. Source: MGI cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay PubMed 18614015. Source: MGI
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Ras-related protein Rab-24		PRO_0000121214
Regions
	Nucleotide binding	14 – 22	9	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	120 – 123	4	GTP By similarity
	Nucleotide binding	154 – 156	3	GTP By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Lipidation	200	1	S-geranylgeranyl cysteine By similarity
	Lipidation	201	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	22	1	S → N: Predominantly in the GTP-bound state. No effect on subcellular location. Ref.5
	Mutagenesis	67	1	S → Q: Increase in GTPase activity. No effect neither on prenylation, nor on subcellular location. Ref.4
	Mutagenesis	120	1	T → A or I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Ref.5
	Mutagenesis	123	1	D → I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Disruptes the integrity of the nuclear envelope. Ref.5
	Sequence conflict	67	1	S → Q in AAK14830. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P35290 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 872EA5E2E7B5B0F0	FASTA	203	23,144
	10 20 30 40 50 60 MSGQRVDVKV VMLGKEYVGK TSLVERYVHD RFLVGPYQNT IGAAFVAKVM CVGDRTVTLG 70 80 90 100 110 120 IWDTAGSERY EAMSRIYYRG AKAAIVCYDL TDSSSFERAK FWVKELRSLE EGCQIYLCGT 130 140 150 160 170 180 KSDLLEEDRR RRRVDFHDVQ DYADNIKAQL FETSSKTGQS VDELFQKVAE DYVSVAAFQV 190 200 MTEDKGVDLS QKANPYFYSC CHH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily." Olkkonen V.M., Dupree P., Killisch I., Luetcke A., Simons K., Zerial M. J. Cell Sci. 106:1249-1261(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon and Mammary gland.
[3]	"The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach." Chavrier P., Simons K., Zerial M. Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68. Tissue: Kidney.
[4]	"Rab24 is an atypical member of the Rab GTPase family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells." Erdman R.A., Shellenberger K.E., Overmeyer J.H., Maltese W.A. J. Biol. Chem. 275:3848-3856(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INEFFICIENT ISOPRENYLATION, LACK OF INTERACTION WITH ARHGDIA AND ARHGDIB, MUTAGENESIS OF SER-67.
[5]	"Mutant Rab24 GTPase is targeted to nuclear inclusions." Maltese W.A., Soule G., Gunning W., Calomeni E., Alexander B. BMC Cell Biol. 3:25-25(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-22; THR-120 AND ASP-123.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z22819 mRNA. Translation: CAA80472.1. BC019950 mRNA. Translation: AAH19950.1. BC054466 mRNA. Translation: AAH54466.1. BC055894 mRNA. Translation: AAH55894.1. M79306 mRNA. Translation: AAK14830.1.
IPI	IPI00116760.
PIR	JH0646. S40235.
RefSeq	NP_033026.1. NM_009000.3.
UniGene	Mm.220923.
3D structure databases
ProteinModelPortal	P35290.
SMR	P35290. Positions 9-170.
ModBase	Search...
PTM databases
PhosphoSite	P35290.
Proteomic databases
PaxDb	P35290.
PRIDE	P35290.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035242; ENSMUSP00000046188; ENSMUSG00000034789.
GeneID	19336.
KEGG	mmu:19336.
Organism-specific databases
CTD	53917.
MGI	MGI:105065. Rab24.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
InParanoid	P35290.
KO	K07912.
OMA	FWVKELQ.
OrthoDB	EOG469QVS.
Gene expression databases
ArrayExpress	P35290.
Bgee	P35290.
CleanEx	MM_RAB24.
Genevestigator	P35290.
GermOnline	ENSMUSG00000034789. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296327.
SOURCE	Search...

Entry information

Entry name

RAB24_MOUSE

Accession

Primary (citable) accession number: P35290

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program