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P35290

- RAB24_MOUSE

UniProt

P35290 - RAB24_MOUSE

Protein

Ras-related protein Rab-24

Gene

Rab24

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    May be involved in autophagy-related processes.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 229GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi120 – 1234GTPBy similarity
    Nucleotide bindingi154 – 1563GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. protein binding Source: MGI

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. protein transport Source: UniProtKB-KW
    3. small GTPase mediated signal transduction Source: InterPro

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-24
    Alternative name(s):
    Rab-16
    Gene namesi
    Name:Rab24
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:105065. Rab24.

    Subcellular locationi

    Cytoplasmcytosol. Membrane; Lipid-anchor
    Note: Only about 20% is recovered in the particulate fraction.

    GO - Cellular componenti

    1. autophagic vacuole Source: MGI
    2. cytosol Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB-SubCell
    4. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi22 – 221S → N: Predominantly in the GTP-bound state. No effect on subcellular location. 1 Publication
    Mutagenesisi67 – 671S → Q: Increase in GTPase activity. No effect neither on prenylation, nor on subcellular location. 1 Publication
    Mutagenesisi120 – 1201T → A or I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. 1 Publication
    Mutagenesisi123 – 1231D → I: Accumulates in punctate structures in both cytoplasmic and nuclear compartments. Disruptes the integrity of the nuclear envelope. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 203203Ras-related protein Rab-24PRO_0000121214Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity
    Lipidationi201 – 2011S-geranylgeranyl cysteineBy similarity

    Post-translational modificationi

    Isoprenylation is inefficient compared to other Rab family members.

    Keywords - PTMi

    Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiP35290.
    PaxDbiP35290.
    PRIDEiP35290.

    PTM databases

    PhosphoSiteiP35290.

    Expressioni

    Tissue specificityi

    Widely expressed, with highest expression in brain.1 Publication

    Gene expression databases

    ArrayExpressiP35290.
    BgeeiP35290.
    CleanExiMM_RAB24.
    GenevestigatoriP35290.

    Interactioni

    Subunit structurei

    Unlike other Rab family members, does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB.

    Protein-protein interaction databases

    IntActiP35290. 1 interaction.
    MINTiMINT-7899035.

    Structurei

    3D structure databases

    ProteinModelPortaliP35290.
    SMRiP35290. Positions 9-170.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP35290.
    KOiK07912.
    OMAiFWVKELQ.
    OrthoDBiEOG7B8S58.
    PhylomeDBiP35290.
    TreeFamiTF300199.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGQRVDVKV VMLGKEYVGK TSLVERYVHD RFLVGPYQNT IGAAFVAKVM    50
    CVGDRTVTLG IWDTAGSERY EAMSRIYYRG AKAAIVCYDL TDSSSFERAK 100
    FWVKELRSLE EGCQIYLCGT KSDLLEEDRR RRRVDFHDVQ DYADNIKAQL 150
    FETSSKTGQS VDELFQKVAE DYVSVAAFQV MTEDKGVDLS QKANPYFYSC 200
    CHH 203
    Length:203
    Mass (Da):23,144
    Last modified:October 1, 1996 - v2
    Checksum:i872EA5E2E7B5B0F0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti67 – 671S → Q in AAK14830. (PubMed:1555775)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22819 mRNA. Translation: CAA80472.1.
    BC019950 mRNA. Translation: AAH19950.1.
    BC054466 mRNA. Translation: AAH54466.1.
    BC055894 mRNA. Translation: AAH55894.1.
    M79306 mRNA. Translation: AAK14830.1.
    CCDSiCCDS26541.1.
    PIRiJH0646.
    S40235.
    RefSeqiNP_033026.1. NM_009000.3.
    UniGeneiMm.220923.

    Genome annotation databases

    EnsembliENSMUST00000035242; ENSMUSP00000046188; ENSMUSG00000034789.
    GeneIDi19336.
    KEGGimmu:19336.
    UCSCiuc007qqk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z22819 mRNA. Translation: CAA80472.1 .
    BC019950 mRNA. Translation: AAH19950.1 .
    BC054466 mRNA. Translation: AAH54466.1 .
    BC055894 mRNA. Translation: AAH55894.1 .
    M79306 mRNA. Translation: AAK14830.1 .
    CCDSi CCDS26541.1.
    PIRi JH0646.
    S40235.
    RefSeqi NP_033026.1. NM_009000.3.
    UniGenei Mm.220923.

    3D structure databases

    ProteinModelPortali P35290.
    SMRi P35290. Positions 9-170.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35290. 1 interaction.
    MINTi MINT-7899035.

    PTM databases

    PhosphoSitei P35290.

    Proteomic databases

    MaxQBi P35290.
    PaxDbi P35290.
    PRIDEi P35290.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035242 ; ENSMUSP00000046188 ; ENSMUSG00000034789 .
    GeneIDi 19336.
    KEGGi mmu:19336.
    UCSCi uc007qqk.1. mouse.

    Organism-specific databases

    CTDi 53917.
    MGIi MGI:105065. Rab24.

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P35290.
    KOi K07912.
    OMAi FWVKELQ.
    OrthoDBi EOG7B8S58.
    PhylomeDBi P35290.
    TreeFami TF300199.

    Miscellaneous databases

    NextBioi 296327.
    PROi P35290.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35290.
    Bgeei P35290.
    CleanExi MM_RAB24.
    Genevestigatori P35290.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily."
      Olkkonen V.M., Dupree P., Killisch I., Luetcke A., Simons K., Zerial M.
      J. Cell Sci. 106:1249-1261(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary gland.
    3. "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
      Chavrier P., Simons K., Zerial M.
      Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-68.
      Tissue: Kidney.
    4. "Rab24 is an atypical member of the Rab GTPase family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells."
      Erdman R.A., Shellenberger K.E., Overmeyer J.H., Maltese W.A.
      J. Biol. Chem. 275:3848-3856(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INEFFICIENT ISOPRENYLATION, LACK OF INTERACTION WITH ARHGDIA AND ARHGDIB, MUTAGENESIS OF SER-67.
    5. "Mutant Rab24 GTPase is targeted to nuclear inclusions."
      Maltese W.A., Soule G., Gunning W., Calomeni E., Alexander B.
      BMC Cell Biol. 3:25-25(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF SER-22; THR-120 AND ASP-123.

    Entry informationi

    Entry nameiRAB24_MOUSE
    AccessioniPrimary (citable) accession number: P35290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The unusual Ser-67, instead of a conserved Gln in other family members, is the cause of low GTPase activity. As a result, the predominant nucleotide associated with the protein is GTP.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3