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Protein

Ras-related protein Rab-23

Gene

Rab23

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes (By similarity). Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity.By similarity5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 238GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi121 – 1244GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • cellular defense response Source: UniProtKB
  • cilium assembly Source: MGI
  • craniofacial suture morphogenesis Source: UniProtKB
  • dorsal/ventral neural tube patterning Source: UniProtKB
  • embryonic digit morphogenesis Source: MGI
  • intracellular protein transport Source: InterPro
  • negative regulation of proteolysis Source: MGI
  • negative regulation of transcription factor import into nucleus Source: UniProtKB
  • nervous system development Source: MGI
  • neural tube closure Source: UniProtKB
  • nucleocytoplasmic transport Source: InterPro
  • regulation of smoothened signaling pathway Source: MGI
  • signal transduction Source: MGI
  • small GTPase mediated signal transduction Source: InterPro
  • spinal cord dorsal/ventral patterning Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-23
Alternative name(s):
Protein open brain
Rab-15
Gene namesi
Name:Rab23
Synonyms:Opb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99833. Rab23.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • endosome membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Rab23 are the cause of the open brain phenotype. Mice suffer from exencephaly and severe malformations of the spinal cord and the dorsal root ganglia, leading to complete embryonic lethality. In addition, mice display poorly developed eyes and polydactyly.

Disruption phenotypei

Complete embryonic lethality, due to defects in neural tube closure. Mice suffer from exencephaly and severe malformations of the spinal cord and the dorsal root ganglia. In addition, mice display poorly developed eyes and polydactyly.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234Ras-related protein Rab-23PRO_0000121212Add
BLAST
Propeptidei235 – 2373Removed in mature formSequence analysisPRO_0000370772

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei234 – 2341Cysteine methyl esterSequence analysis
Lipidationi234 – 2341S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP35288.
MaxQBiP35288.
PaxDbiP35288.
PRIDEiP35288.

PTM databases

iPTMnetiP35288.

Expressioni

Tissue specificityi

Detected in brain neurons (at protein level). Forebrain and midbrain.2 Publications

Gene expression databases

CleanExiMM_RAB23.

Interactioni

Subunit structurei

Interacts with SUFU.By similarity

Protein-protein interaction databases

DIPiDIP-60517N.
STRINGi10090.ENSMUSP00000085625.

Structurei

Secondary structure

1
237
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 157Combined sources
Helixi22 – 3110Combined sources
Beta strandi43 – 5311Combined sources
Beta strandi56 – 638Combined sources
Helixi68 – 703Combined sources
Helixi76 – 794Combined sources
Beta strandi84 – 907Combined sources
Helixi94 – 985Combined sources
Helixi100 – 11112Combined sources
Beta strandi116 – 1216Combined sources
Helixi123 – 1286Combined sources
Helixi133 – 14311Combined sources
Beta strandi146 – 1494Combined sources
Turni152 – 1554Combined sources
Beta strandi156 – 1583Combined sources
Helixi159 – 17012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z22X-ray2.06A7-172[»]
1Z2AX-ray1.90A7-172[»]
ProteinModelPortaliP35288.
SMRiP35288. Positions 8-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35288.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG4252. Eukaryota.
ENOG4110BDM. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG100144.
InParanoidiP35288.
PhylomeDBiP35288.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEEDMEVAI KMVVVGNGAV GKSSMIQRYC KGIFTKDYKK TIGVDFLERQ
60 70 80 90 100
IQVNDEDVRL MLWDTAGQEE FDAITKAYYR GAQACVLVFS TTDRESFEAI
110 120 130 140 150
SSWREKVVAE VGDIPTALVQ NKIDLLDDSC IKNEEAEGLA KRLKLRFYRT
160 170 180 190 200
SVKEDLNVSE VFKYLAEKHL QKLKQQITED PEQTHSSSNK IGVFNASVGS
210 220 230
HLGQNSSSLN GGDVINLRPN KQRTKRTRNP FSSCSVP
Length:237
Mass (Da):26,678
Last modified:October 1, 1996 - v2
Checksum:iAA1F2B25BAECD3E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22821 mRNA. Translation: CAA80474.1.
BC025578 mRNA. Translation: AAH25578.1.
M79305 mRNA. Translation: AAK14829.1.
CCDSiCCDS35532.1.
PIRiI48729. S40244.
JH0645.
UniGeneiMm.86744.

Genome annotation databases

UCSCiuc007anv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22821 mRNA. Translation: CAA80474.1.
BC025578 mRNA. Translation: AAH25578.1.
M79305 mRNA. Translation: AAK14829.1.
CCDSiCCDS35532.1.
PIRiI48729. S40244.
JH0645.
UniGeneiMm.86744.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z22X-ray2.06A7-172[»]
1Z2AX-ray1.90A7-172[»]
ProteinModelPortaliP35288.
SMRiP35288. Positions 8-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60517N.
STRINGi10090.ENSMUSP00000085625.

PTM databases

iPTMnetiP35288.

Proteomic databases

EPDiP35288.
MaxQBiP35288.
PaxDbiP35288.
PRIDEiP35288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007anv.1. mouse.

Organism-specific databases

MGIiMGI:99833. Rab23.

Phylogenomic databases

eggNOGiKOG4252. Eukaryota.
ENOG4110BDM. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG100144.
InParanoidiP35288.
PhylomeDBiP35288.

Miscellaneous databases

EvolutionaryTraceiP35288.
PROiP35288.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RAB23.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a mouse cDNA encoding Rab23, a small novel GTPase expressed predominantly in the brain."
    Olkkonen V.M., Peterson J.R., Dupree P., Lutcke A., Zerial M., Simons K.
    Gene 138:207-211(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "The complexity of the Rab and Rho GTP-binding protein subfamilies revealed by a PCR cloning approach."
    Chavrier P., Simons K., Zerial M.
    Gene 112:261-264(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-69.
    Tissue: Kidney.
  4. "Open brain, a new mouse mutant with severe neural tube defects, shows altered gene expression patterns in the developing spinal cord."
    Gunther T., Struwe M., Aguzzi A., Schughart K.
    Development 120:3119-3130(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN DISEASE.
  5. "Dorsal and lateral fates in the mouse neural tube require the cell-autonomous activity of the open brain gene."
    Eggenschwiler J.T., Anderson K.V.
    Dev. Biol. 227:648-660(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "Rab23 is an essential negative regulator of the mouse Sonic hedgehog signalling pathway."
    Eggenschwiler J.T., Espinoza E., Anderson K.V.
    Nature 412:194-198(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  7. "Rab23, a negative regulator of hedgehog signaling, localizes to the plasma membrane and the endocytic pathway."
    Evans T.M., Ferguson C., Wainwright B.J., Parton R.G., Wicking C.
    Traffic 4:869-884(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Mouse Rab23 regulates hedgehog signaling from smoothened to Gli proteins."
    Eggenschwiler J.T., Bulgakov O.V., Qin J., Li T., Anderson K.V.
    Dev. Biol. 290:1-12(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Open brain gene product Rab23: expression pattern in the adult mouse brain and functional characterization."
    Guo A., Wang T., Ng E.L., Aulia S., Chong K.H., Teng F.Y., Wang Y., Tang B.L.
    J. Neurosci. Res. 83:1118-1127(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  10. Cited for: FUNCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Lung and Testis.
  12. "Structural basis of family-wide Rab GTPase recognition by rabenosyn-5."
    Eathiraj S., Pan X., Ritacco C., Lambright D.G.
    Nature 436:415-419(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 7-172.

Entry informationi

Entry nameiRAB23_MOUSE
AccessioniPrimary (citable) accession number: P35288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.