P35286 (RAB13_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 122. History...
Names and origin
|Protein names||Recommended name:|
Ras-related protein Rab-13
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||203 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. Ref.3 Ref.4 Ref.7
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab may be activated by DENND1C, a guanine exchange factor By similarity. Activated in response to insulin. Ref.4
Interacts (GTP-bound form) with MICALL2; competes with RAB8A and is involved in tight junctions assembly. Interacts (GTP-bound form) with MICALL1. Interacts with PRKACA; downstream effector of RAB13 involved in tight junction assembly. Interacts with GRB2; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts (isoprenylated form) with PDE6D; dissociates RAB13 from membranes. Interacts with CCDC64B/BICDR2. Interacts with LEPROT and LEPROTL1. Ref.6 Ref.7
Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side By similarity. Cell junction › tight junction By similarity. Golgi apparatus › trans-Golgi network membrane By similarity. Recycling endosome membrane By similarity. Cell projection › lamellipodium By similarity. Cytoplasmic vesicle › secretory vesicle membrane By similarity. Note: Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions By similarity. Relocalizes to the leading edge of lamellipodia in migrating endothelial cells By similarity.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 200||200||Ras-related protein Rab-13||PRO_0000121184|
|Propeptide||201 – 203||3||Removed in mature form Potential||PRO_0000370759|
|Nucleotide binding||15 – 22||8||GTP By similarity|
|Nucleotide binding||63 – 67||5||GTP By similarity|
|Nucleotide binding||121 – 124||4||GTP By similarity|
|Motif||37 – 45||9||Effector region By similarity|
Amino acid modifications
|Modified residue||178||1||Phosphoserine By similarity|
|Modified residue||200||1||Cysteine methyl ester Potential|
|Lipidation||200||1||S-geranylgeranyl cysteine By similarity|
|Sequence conflict||76||1||A → S in AAA41993. Ref.2|
|Sequence conflict||113||1||G → E in AAA41993. Ref.2|
|||"RAB13 participates in ectoplasmic specialization dynamics in the rat testis."|
Mruk D.D., Lau A.S.
Biol. Reprod. 80:590-601(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
|||"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."|
Elferink L.A., Anzai K., Scheller R.H.
J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-203.
|||"Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2."|
Sakane A., Honda K., Sasaki T.
Mol. Cell. Biol. 30:1077-1087(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN NEURITE OUTGROWTH.
|||"Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."|
Sun Y., Bilan P.J., Liu Z., Klip A.
Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.
|||"Rab13 is upregulated during osteoclast differentiation and associates with small vesicles revealing polarized distribution in resorbing cells."|
Hirvonen M.J., Mulari M.T., Bueki K.G., Vihko P., Haerkoenen P.L., Vaeaenaenen H.K.
J. Histochem. Cytochem. 60:537-549(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
|||"Novel interaction of Rab13 and Rab8 with endospanins."|
Hirvonen M.J., Bueki K.G., Sun Y., Mulari M.T., Haerkoenen P.L., Vaeaenaenen K.H.
FEBS Open Bio 3:83-88(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LEPROT AND LEPROTL1.
|||"RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A."|
Su W., Liu X.
J. Mol. Endocrinol. 50:305-318(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PRKACA.
|+||Additional computationally mapped references.|
|AF525280 mRNA. Translation: AAM82588.1.|
M83678 mRNA. Translation: AAA41993.1.
|RefSeq||NP_112354.1. NM_031092.1. |
3D structure databases
|SMR||P35286. Positions 8-169. |
Protein-protein interaction databases
|IntAct||P35286. 4 interactions.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000022627; ENSRNOP00000022626; ENSRNOG00000016733. |
|UCSC||RGD:620880. rat. |
|RGD||620880. Rab13. |
Gene expression databases
Family and domain databases
|Gene3D||184.108.40.2060. 1 hit. |
|InterPro||IPR027417. P-loop_NTPase. |
|Pfam||PF00071. Ras. 1 hit. |
|PRINTS||PR00449. RASTRNSFRMNG. |
|SMART||SM00175. RAB. 1 hit. |
|SUPFAM||SSF52540. SSF52540. 1 hit. |
|TIGRFAMs||TIGR00231. small_GTP. 1 hit. |
|PROSITE||PS51419. RAB. 1 hit. |
|Accession||Primary (citable) accession number: P35286|
Secondary accession number(s): Q8K3X5
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families