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P35286

- RAB13_RAT

UniProt

P35286 - RAB13_RAT

Protein

Ras-related protein Rab-13

Gene

Rab13

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (26 Apr 2004)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.3 Publications

    Enzyme regulationi

    Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab may be activated by DENND1C, a guanine exchange factor By similarity. Activated in response to insulin.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi121 – 1244GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: Ensembl
    2. GTP binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein kinase A catalytic subunit binding Source: UniProtKB

    GO - Biological processi

    1. cellular response to insulin stimulus Source: UniProtKB
    2. cortical actin cytoskeleton organization Source: UniProtKB
    3. endocytic recycling Source: UniProtKB
    4. endosomal transport Source: UniProtKB
    5. endothelial cell chemotaxis Source: UniProtKB
    6. establishment of protein localization to plasma membrane Source: UniProtKB
    7. establishment of Sertoli cell barrier Source: UniProtKB
    8. glucose homeostasis Source: UniProtKB
    9. neuron projection development Source: UniProtKB
    10. positive regulation of glucose import Source: UniProtKB
    11. protein kinase A signaling Source: UniProtKB
    12. protein localization to cell leading edge Source: UniProtKB
    13. protein localization to plasma membrane Source: UniProtKB
    14. protein transport Source: UniProtKB-KW
    15. small GTPase mediated signal transduction Source: InterPro
    16. tight junction assembly Source: UniProtKB
    17. trans-Golgi network to recycling endosome transport Source: UniProtKB

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199176. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-13
    Gene namesi
    Name:Rab13
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi620880. Rab13.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Cytoplasmic vesicle membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity. Cell junctiontight junction By similarity. Golgi apparatustrans-Golgi network membrane By similarity. Recycling endosome membrane By similarity. Cell projectionlamellipodium By similarity. Cytoplasmic vesiclesecretory vesicle membrane By similarity
    Note: Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions. Relocalizes to the leading edge of lamellipodia in migrating endothelial cells.By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: Reactome
    2. endocytic vesicle Source: UniProtKB
    3. insulin-responsive compartment Source: UniProtKB
    4. lamellipodium Source: UniProtKB
    5. lateral plasma membrane Source: UniProtKB
    6. neuron projection Source: UniProtKB
    7. plasma membrane Source: UniProtKB
    8. recycling endosome Source: UniProtKB
    9. recycling endosome membrane Source: UniProtKB-SubCell
    10. tight junction Source: UniProtKB
    11. trans-Golgi network Source: UniProtKB
    12. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Tight junction

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Ras-related protein Rab-13PRO_0000121184Add
    BLAST
    Propeptidei201 – 2033Removed in mature formSequence AnalysisPRO_0000370759

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei178 – 1781PhosphoserineBy similarity
    Modified residuei200 – 2001Cysteine methyl esterSequence Analysis
    Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiP35286.
    PRIDEiP35286.

    PTM databases

    PhosphoSiteiP35286.

    Expressioni

    Tissue specificityi

    Highest levels found in lung, kidney, whole brain and spinal cord. Expressed in all tissues tested including Sertoli and germ cells (at protein level). Also detected in osteoclasts.2 Publications

    Gene expression databases

    GenevestigatoriP35286.

    Interactioni

    Subunit structurei

    Interacts (GTP-bound form) with MICALL2; competes with RAB8A and is involved in tight junctions assembly. Interacts (GTP-bound form) with MICALL1. Interacts with PRKACA; downstream effector of RAB13 involved in tight junction assembly. Interacts with GRB2; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts (isoprenylated form) with PDE6D; dissociates RAB13 from membranes. Interacts with CCDC64B/BICDR2. Interacts with LEPROT and LEPROTL1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Leprotl1Q6PDU43EBI-917332,EBI-8702651

    Protein-protein interaction databases

    IntActiP35286. 4 interactions.
    MINTiMINT-2982767.
    STRINGi10116.ENSRNOP00000022626.

    Structurei

    3D structure databases

    ProteinModelPortaliP35286.
    SMRiP35286. Positions 8-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117504.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiP35286.
    KOiK06109.
    OMAiKVQREQA.
    OrthoDBiEOG7VB2H4.
    PhylomeDBiP35286.
    TreeFamiTF314097.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35286-1 [UniParc]FASTAAdd to Basket

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    MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNSTYIST IGIDFKIRTV    50
    EIEGKRIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ 100
    NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQREQAERLA REHRIRFFET 150
    SAKSSVNVDE AFSSLARDIL LKTGGRRSGN SSKPSSTDLK VSDKKNSNKC 200
    SLG 203
    Length:203
    Mass (Da):22,901
    Last modified:April 26, 2004 - v2
    Checksum:iB6687462C6EA95A4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti76 – 761A → S in AAA41993. (PubMed:1313420)Curated
    Sequence conflicti113 – 1131G → E in AAA41993. (PubMed:1313420)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525280 mRNA. Translation: AAM82588.1.
    M83678 mRNA. Translation: AAA41993.1.
    PIRiD42148.
    RefSeqiNP_112354.1. NM_031092.1.
    UniGeneiRn.9819.

    Genome annotation databases

    EnsembliENSRNOT00000022627; ENSRNOP00000022626; ENSRNOG00000016733.
    GeneIDi81756.
    KEGGirno:81756.
    UCSCiRGD:620880. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF525280 mRNA. Translation: AAM82588.1 .
    M83678 mRNA. Translation: AAA41993.1 .
    PIRi D42148.
    RefSeqi NP_112354.1. NM_031092.1.
    UniGenei Rn.9819.

    3D structure databases

    ProteinModelPortali P35286.
    SMRi P35286. Positions 8-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35286. 4 interactions.
    MINTi MINT-2982767.
    STRINGi 10116.ENSRNOP00000022626.

    PTM databases

    PhosphoSitei P35286.

    Proteomic databases

    PaxDbi P35286.
    PRIDEi P35286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000022627 ; ENSRNOP00000022626 ; ENSRNOG00000016733 .
    GeneIDi 81756.
    KEGGi rno:81756.
    UCSCi RGD:620880. rat.

    Organism-specific databases

    CTDi 5872.
    RGDi 620880. Rab13.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117504.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi P35286.
    KOi K06109.
    OMAi KVQREQA.
    OrthoDBi EOG7VB2H4.
    PhylomeDBi P35286.
    TreeFami TF314097.

    Enzyme and pathway databases

    Reactomei REACT_199176. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    NextBioi 615526.
    PROi P35286.

    Gene expression databases

    Genevestigatori P35286.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "RAB13 participates in ectoplasmic specialization dynamics in the rat testis."
      Mruk D.D., Lau A.S.
      Biol. Reprod. 80:590-601(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
    2. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
      Elferink L.A., Anzai K., Scheller R.H.
      J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-203.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2."
      Sakane A., Honda K., Sasaki T.
      Mol. Cell. Biol. 30:1077-1087(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN NEURITE OUTGROWTH.
    4. "Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
      Sun Y., Bilan P.J., Liu Z., Klip A.
      Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.
    5. "Rab13 is upregulated during osteoclast differentiation and associates with small vesicles revealing polarized distribution in resorbing cells."
      Hirvonen M.J., Mulari M.T., Bueki K.G., Vihko P., Haerkoenen P.L., Vaeaenaenen H.K.
      J. Histochem. Cytochem. 60:537-549(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. Cited for: INTERACTION WITH LEPROT AND LEPROTL1.
    7. "RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A."
      Su W., Liu X.
      J. Mol. Endocrinol. 50:305-318(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PRKACA.

    Entry informationi

    Entry nameiRAB13_RAT
    AccessioniPrimary (citable) accession number: P35286
    Secondary accession number(s): Q8K3X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: April 26, 2004
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3