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P35286

- RAB13_RAT

UniProt

P35286 - RAB13_RAT

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Protein
Ras-related protein Rab-13
Gene
Rab13
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.3 Publications

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab may be activated by DENND1C, a guanine exchange factor By similarity. Activated in response to insulin.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTP By similarity
Nucleotide bindingi63 – 675GTP By similarity
Nucleotide bindingi121 – 1244GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB
  2. GTPase activity Source: Ensembl
  3. protein binding Source: IntAct
  4. protein kinase A catalytic subunit binding Source: UniProtKB

GO - Biological processi

  1. cellular response to insulin stimulus Source: UniProtKB
  2. cortical actin cytoskeleton organization Source: UniProtKB
  3. endocytic recycling Source: UniProtKB
  4. endosomal transport Source: UniProtKB
  5. endothelial cell chemotaxis Source: UniProtKB
  6. establishment of Sertoli cell barrier Source: UniProtKB
  7. establishment of protein localization to plasma membrane Source: UniProtKB
  8. glucose homeostasis Source: UniProtKB
  9. neuron projection development Source: UniProtKB
  10. positive regulation of glucose import Source: UniProtKB
  11. protein kinase A signaling Source: UniProtKB
  12. protein localization to cell leading edge Source: UniProtKB
  13. protein localization to plasma membrane Source: UniProtKB
  14. protein transport Source: UniProtKB-KW
  15. small GTPase mediated signal transduction Source: InterPro
  16. tight junction assembly Source: UniProtKB
  17. trans-Golgi network to recycling endosome transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_199176. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-13
Gene namesi
Name:Rab13
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi620880. Rab13.

Subcellular locationi

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side By similarity. Cell junctiontight junction By similarity. Golgi apparatustrans-Golgi network membrane By similarity. Recycling endosome membrane By similarity. Cell projectionlamellipodium By similarity. Cytoplasmic vesiclesecretory vesicle membrane By similarity
Note: Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions By similarity. Relocalizes to the leading edge of lamellipodia in migrating endothelial cells By similarity.

GO - Cellular componenti

  1. cytoplasmic vesicle membrane Source: Reactome
  2. endocytic vesicle Source: UniProtKB
  3. insulin-responsive compartment Source: UniProtKB
  4. lamellipodium Source: UniProtKB
  5. lateral plasma membrane Source: UniProtKB
  6. neuron projection Source: UniProtKB
  7. plasma membrane Source: UniProtKB
  8. recycling endosome Source: UniProtKB
  9. recycling endosome membrane Source: UniProtKB-SubCell
  10. tight junction Source: UniProtKB
  11. trans-Golgi network Source: UniProtKB
  12. transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Ras-related protein Rab-13
PRO_0000121184Add
BLAST
Propeptidei201 – 2033Removed in mature form Reviewed prediction
PRO_0000370759

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781Phosphoserine By similarity
Modified residuei200 – 2001Cysteine methyl ester Reviewed prediction
Lipidationi200 – 2001S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP35286.
PRIDEiP35286.

PTM databases

PhosphoSiteiP35286.

Expressioni

Tissue specificityi

Highest levels found in lung, kidney, whole brain and spinal cord. Expressed in all tissues tested including Sertoli and germ cells (at protein level). Also detected in osteoclasts.2 Publications

Gene expression databases

GenevestigatoriP35286.

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL2; competes with RAB8A and is involved in tight junctions assembly. Interacts (GTP-bound form) with MICALL1. Interacts with PRKACA; downstream effector of RAB13 involved in tight junction assembly. Interacts with GRB2; may recruit RAB13 to the leading edge of migrating endothelial cells where it can activate RHOA. Interacts (isoprenylated form) with PDE6D; dissociates RAB13 from membranes. Interacts with CCDC64B/BICDR2. Interacts with LEPROT and LEPROTL1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Leprotl1Q6PDU43EBI-917332,EBI-8702651

Protein-protein interaction databases

IntActiP35286. 4 interactions.
MINTiMINT-2982767.
STRINGi10116.ENSRNOP00000022626.

Structurei

3D structure databases

ProteinModelPortaliP35286.
SMRiP35286. Positions 8-169.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector region By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00750000117504.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35286.
KOiK06109.
OMAiKVQREQA.
OrthoDBiEOG7VB2H4.
PhylomeDBiP35286.
TreeFamiTF314097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35286-1 [UniParc]FASTAAdd to Basket

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MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNSTYIST IGIDFKIRTV    50
EIEGKRIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ 100
NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQREQAERLA REHRIRFFET 150
SAKSSVNVDE AFSSLARDIL LKTGGRRSGN SSKPSSTDLK VSDKKNSNKC 200
SLG 203
Length:203
Mass (Da):22,901
Last modified:April 26, 2004 - v2
Checksum:iB6687462C6EA95A4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761A → S in AAA41993. 1 Publication
Sequence conflicti113 – 1131G → E in AAA41993. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF525280 mRNA. Translation: AAM82588.1.
M83678 mRNA. Translation: AAA41993.1.
PIRiD42148.
RefSeqiNP_112354.1. NM_031092.1.
UniGeneiRn.9819.

Genome annotation databases

EnsembliENSRNOT00000022627; ENSRNOP00000022626; ENSRNOG00000016733.
GeneIDi81756.
KEGGirno:81756.
UCSCiRGD:620880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF525280 mRNA. Translation: AAM82588.1 .
M83678 mRNA. Translation: AAA41993.1 .
PIRi D42148.
RefSeqi NP_112354.1. NM_031092.1.
UniGenei Rn.9819.

3D structure databases

ProteinModelPortali P35286.
SMRi P35286. Positions 8-169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35286. 4 interactions.
MINTi MINT-2982767.
STRINGi 10116.ENSRNOP00000022626.

PTM databases

PhosphoSitei P35286.

Proteomic databases

PaxDbi P35286.
PRIDEi P35286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000022627 ; ENSRNOP00000022626 ; ENSRNOG00000016733 .
GeneIDi 81756.
KEGGi rno:81756.
UCSCi RGD:620880. rat.

Organism-specific databases

CTDi 5872.
RGDi 620880. Rab13.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00750000117504.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P35286.
KOi K06109.
OMAi KVQREQA.
OrthoDBi EOG7VB2H4.
PhylomeDBi P35286.
TreeFami TF314097.

Enzyme and pathway databases

Reactomei REACT_199176. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi 615526.
PROi P35286.

Gene expression databases

Genevestigatori P35286.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "RAB13 participates in ectoplasmic specialization dynamics in the rat testis."
    Mruk D.D., Lau A.S.
    Biol. Reprod. 80:590-601(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  2. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 40-203.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2."
    Sakane A., Honda K., Sasaki T.
    Mol. Cell. Biol. 30:1077-1087(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN NEURITE OUTGROWTH.
  4. "Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
    Sun Y., Bilan P.J., Liu Z., Klip A.
    Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.
  5. "Rab13 is upregulated during osteoclast differentiation and associates with small vesicles revealing polarized distribution in resorbing cells."
    Hirvonen M.J., Mulari M.T., Bueki K.G., Vihko P., Haerkoenen P.L., Vaeaenaenen H.K.
    J. Histochem. Cytochem. 60:537-549(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: INTERACTION WITH LEPROT AND LEPROTL1.
  7. "RAB13 regulates Sertoli cell permeability barrier dynamics through protein kinase A."
    Su W., Liu X.
    J. Mol. Endocrinol. 50:305-318(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRKACA.

Entry informationi

Entry nameiRAB13_RAT
AccessioniPrimary (citable) accession number: P35286
Secondary accession number(s): Q8K3X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 26, 2004
Last modified: September 3, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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