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Protein

Ras-related protein Rab-22A

Gene

Rab22a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth (By similarity). Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 209GTP
Nucleotide bindingi60 – 645GTP
Nucleotide bindingi118 – 1214GTP
Nucleotide bindingi148 – 1503GTP

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-22A
Short name:
Rab-22
Alternative name(s):
Rab-14
Gene namesi
Name:Rab22a
Synonyms:Rab22
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:105072. Rab22a.

Subcellular locationi

  • Endosome membrane By similarity; Lipid-anchor Curated
  • Cell membrane By similarity; Lipid-anchor Curated
  • Early endosome 1 Publication
  • Late endosome By similarity
  • Cell projectionruffle By similarity
  • Cytoplasmic vesicle Curated
  • Cytoplasmic vesiclephagosome By similarity
  • Cytoplasmic vesiclephagosome membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

  • Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Ras-related protein Rab-22APRO_0000121210Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi193 – 1931S-geranylgeranyl cysteineBy similarity
Lipidationi194 – 1941S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

EPDiP35285.
PaxDbiP35285.
PeptideAtlasiP35285.
PRIDEiP35285.

PTM databases

iPTMnetiP35285.
PhosphoSiteiP35285.

Expressioni

Tissue specificityi

Detected in brain and heart, and at lower levels in lung and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000027519.
CleanExiMM_RAB22A.
ExpressionAtlasiP35285. baseline and differential.
GenevisibleiP35285. MM.

Interactioni

Subunit structurei

Binds EEA1. Interacts (in its GTP-bound form) with RINL (By similarity). Interacts directly with ZFYVE20. Interacts (in its GTP-bound form) with RABGEF1.By similarity2 Publications

Protein-protein interaction databases

BioGridi202539. 1 interaction.
DIPiDIP-60516N.
IntActiP35285. 34 interactions.
MINTiMINT-4131884.
STRINGi10090.ENSMUSP00000029024.

Structurei

Secondary structure

1
194
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 1110Combined sources
Helixi18 – 2710Combined sources
Beta strandi39 – 4911Combined sources
Beta strandi52 – 6110Combined sources
Helixi65 – 717Combined sources
Helixi72 – 754Combined sources
Beta strandi79 – 868Combined sources
Helixi90 – 10617Combined sources
Beta strandi111 – 1188Combined sources
Helixi123 – 1253Combined sources
Helixi130 – 13910Combined sources
Beta strandi143 – 1464Combined sources
Turni149 – 1524Combined sources
Helixi155 – 16511Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVDX-ray1.93A3-169[»]
1Z0JX-ray1.32A2-169[»]
ProteinModelPortaliP35285.
SMRiP35285. Positions 2-167.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35285.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 429Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35285.
KOiK07891.
OMAiKCDLSDA.
OrthoDBiEOG091G0HKN.
PhylomeDBiP35285.
TreeFamiTF331262.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRELKVCL LGDTGVGKSS IVWRFVEDSF DPNINPTIGA SFMTKTVQYQ
60 70 80 90 100
NELHKFLIWD TAGQERFRAL APMYYRGSAA AIIVYDITKE ETFSTLKNWV
110 120 130 140 150
RELRQHGPPS IVVAIAGNKC DLTDVREVME RDAKDYADSI HAIFVETSAK
160 170 180 190
NAININELFI EISRRIPSTD ANPASGGKGF KLRRQPSEPK RSCC
Length:194
Mass (Da):21,802
Last modified:April 26, 2004 - v2
Checksum:i1799B676CA9091F7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541H → R in AAK14828 (PubMed:1555775).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311124 mRNA. Translation: CAC41378.1.
AK031667 mRNA. Translation: BAC27501.1.
AK146594 mRNA. Translation: BAE27289.1.
AK150617 mRNA. Translation: BAE29707.1.
BC006596 mRNA. Translation: AAH06596.1.
M79304 mRNA. Translation: AAK14828.1.
CCDSiCCDS38352.1.
PIRiJH0644.
RefSeqiNP_077756.2. NM_024436.3.
UniGeneiMm.275304.

Genome annotation databases

EnsembliENSMUST00000029024; ENSMUSP00000029024; ENSMUSG00000027519.
GeneIDi19334.
KEGGimmu:19334.
UCSCiuc008oed.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ311124 mRNA. Translation: CAC41378.1.
AK031667 mRNA. Translation: BAC27501.1.
AK146594 mRNA. Translation: BAE27289.1.
AK150617 mRNA. Translation: BAE29707.1.
BC006596 mRNA. Translation: AAH06596.1.
M79304 mRNA. Translation: AAK14828.1.
CCDSiCCDS38352.1.
PIRiJH0644.
RefSeqiNP_077756.2. NM_024436.3.
UniGeneiMm.275304.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVDX-ray1.93A3-169[»]
1Z0JX-ray1.32A2-169[»]
ProteinModelPortaliP35285.
SMRiP35285. Positions 2-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202539. 1 interaction.
DIPiDIP-60516N.
IntActiP35285. 34 interactions.
MINTiMINT-4131884.
STRINGi10090.ENSMUSP00000029024.

PTM databases

iPTMnetiP35285.
PhosphoSiteiP35285.

Proteomic databases

EPDiP35285.
PaxDbiP35285.
PeptideAtlasiP35285.
PRIDEiP35285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029024; ENSMUSP00000029024; ENSMUSG00000027519.
GeneIDi19334.
KEGGimmu:19334.
UCSCiuc008oed.1. mouse.

Organism-specific databases

CTDi57403.
MGIiMGI:105072. Rab22a.

Phylogenomic databases

eggNOGiKOG0092. Eukaryota.
ENOG410YCCP. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35285.
KOiK07891.
OMAiKCDLSDA.
OrthoDBiEOG091G0HKN.
PhylomeDBiP35285.
TreeFamiTF331262.

Miscellaneous databases

EvolutionaryTraceiP35285.
PROiP35285.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027519.
CleanExiMM_RAB22A.
ExpressionAtlasiP35285. baseline and differential.
GenevisibleiP35285. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB22A_MOUSE
AccessioniPrimary (citable) accession number: P35285
Secondary accession number(s): Q3UCA7, Q91VD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: April 26, 2004
Last modified: September 7, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.