P35281 (RAB10_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 99. History...
Names and origin
|Protein names||Recommended name:|
Ras-related protein Rab-10
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||200 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Ref.3
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the DENND4C guanine exchange factor (GEF) By similarity.
Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1. Ref.3
Cytoplasmic vesicle membrane By similarity; Lipid-anchor Probable; Cytoplasmic side Probable. Golgi apparatus › trans-Golgi network membrane By similarity. Endosome membrane By similarity. Recycling endosome membrane By similarity. Cytoplasmic vesicle › phagosome membrane By similarity. Cell projection › cilium. Endoplasmic reticulum membrane By similarity. Note: Associates with SLC2A4/GLUT4 storage vesicles By similarity. Localizes to the base of the cilium. Transiently associates with phagosomes By similarity. Localizes to the endoplasmic reticulum at domains of new tubule growth By similarity. Ref.2
Highest levels in neural and muscle tissues.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 200||200||Ras-related protein Rab-10||PRO_0000121148|
|Nucleotide binding||16 – 23||8||GTP By similarity|
|Nucleotide binding||64 – 68||5||GTP By similarity|
|Nucleotide binding||122 – 125||4||GTP By similarity|
|Motif||38 – 46||9||Effector region By similarity|
Amino acid modifications
|Modified residue||102||1||N6-acetyllysine By similarity|
|Lipidation||199||1||S-geranylgeranyl cysteine By similarity|
|Lipidation||200||1||S-geranylgeranyl cysteine By similarity|
|||"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."|
Elferink L.A., Anzai K., Scheller R.H.
J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells."|
Babbey C.M., Bacallao R.L., Dunn K.W.
Am. J. Physiol. 299:F495-506(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
|||"Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization."|
Wang T., Liu Y., Xu X.H., Deng C.Y., Wu K.Y., Zhu J., Fu X.Q., He M., Luo Z.G.
Dev. Cell 21:431-444(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN AXON DEVELOPMENT, INTERACTION WITH LLGL1 AND GDI1.
|+||Additional computationally mapped references.|
|M83677 mRNA. Translation: AAA41991.1.|
3D structure databases
|SMR||P35281. Positions 4-173. |
Protein-protein interaction databases
|IntAct||P35281. 2 interactions.|
Protocols and materials databases
|RGD||620879. Rab10. |
Gene expression databases
Family and domain databases
|Gene3D||22.214.171.1240. 1 hit. |
|InterPro||IPR027417. P-loop_NTPase. |
|Pfam||PF00071. Ras. 1 hit. |
|PRINTS||PR00449. RASTRNSFRMNG. |
|SMART||SM00175. RAB. 1 hit. |
|SUPFAM||SSF52540. SSF52540. 1 hit. |
|TIGRFAMs||TIGR00231. small_GTP. 1 hit. |
|PROSITE||PS51419. RAB. 1 hit. |
|Accession||Primary (citable) accession number: P35281|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families