P35281 (RAB10_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-10

Gene names

Name:

Rab10

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Ref.3
Enzyme regulation	Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the DENND4C guanine exchange factor (GEF) By similarity.
Subunit structure	Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1. Ref.3
Subcellular location	Cytoplasmic vesicle membrane By similarity; Lipid-anchor Probable; Cytoplasmic side Probable. Golgi apparatus › trans-Golgi network membrane By similarity. Endosome membrane By similarity. Recycling endosome membrane By similarity. Cytoplasmic vesicle › phagosome membrane By similarity. Cell projection › cilium. Endoplasmic reticulum membrane By similarity. Note: Associates with SLC2A4/GLUT4 storage vesicles By similarity. Localizes to the base of the cilium. Transiently associates with phagosomes By similarity. Localizes to the endoplasmic reticulum at domains of new tubule growth By similarity. Ref.2
Tissue specificity	Highest levels in neural and muscle tissues.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell projection Cytoplasmic vesicle Endoplasmic reticulum Endosome Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane protein transport Inferred from sequence or structural similarity. Source: UniProtKB axonogenesis Inferred from mutant phenotype Ref.3. Source: UniProtKB basolateral protein localization Inferred from sequence or structural similarity. Source: UniProtKB cellular response to antibiotic Inferred from expression pattern PubMed 7688123. Source: RGD cellular response to insulin stimulus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum tubular network organization Inferred from sequence or structural similarity. Source: UniProtKB endosomal transport Inferred from sequence or structural similarity. Source: UniProtKB establishment of neuroblast polarity Inferred from mutant phenotype Ref.3. Source: UniProtKB establishment of protein localization to endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB polarized epithelial cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB protein localization to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB endoplasmic reticulum tubular network Inferred from sequence or structural similarity. Source: UniProtKB insulin-responsive compartment Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay PubMed 7688123. Source: RGD phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell primary cilium Inferred from direct assay Ref.2. Source: UniProtKB recycling endosome Inferred from sequence or structural similarity. Source: UniProtKB recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Inferred from direct assay PubMed 7688123. Source: RGD
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Ras-related protein Rab-10		PRO_0000121148
Regions
	Nucleotide binding	16 – 23	8	GTP By similarity
	Nucleotide binding	64 – 68	5	GTP By similarity
	Nucleotide binding	122 – 125	4	GTP By similarity
	Motif	38 – 46	9	Effector region By similarity
Amino acid modifications
	Modified residue	102	1	N6-acetyllysine By similarity
	Lipidation	199	1	S-geranylgeranyl cysteine By similarity
	Lipidation	200	1	S-geranylgeranyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35281 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EFAF804FD4C3B6A3	FASTA	200	22,858
	10 20 30 40 50 60 MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIEIDFKIKT VELQGKKIKL 70 80 90 100 110 120 QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDQH ANEDVERMLL 130 140 150 160 170 180 RNKCDMDHKR VVPKGKGEQI AREHRIRFFE TSAKANINIE KAFLTLPEDI LRKTPVKEPN 190 200 SENVDISSGG GVTGWKSKCC

References

[1]	"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain." Elferink L.A., Anzai K., Scheller R.H. J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	"Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells." Babbey C.M., Bacallao R.L., Dunn K.W. Am. J. Physiol. 299:F495-506(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization." Wang T., Liu Y., Xu X.H., Deng C.Y., Wu K.Y., Zhu J., Fu X.Q., He M., Luo Z.G. Dev. Cell 21:431-444(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN AXON DEVELOPMENT, INTERACTION WITH LLGL1 AND GDI1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83677 mRNA. Translation: AAA41991.1.
IPI	IPI00196790.
PIR	B42148.
UniGene	Rn.65864.
3D structure databases
ProteinModelPortal	P35281.
SMR	P35281. Positions 4-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35281. 1 interaction.
PTM databases
PhosphoSite	P35281.
Proteomic databases
PRIDE	P35281.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	620879. Rab10.
Phylogenomic databases
HOVERGEN	HBG009351.
Gene expression databases
Genevestigator	P35281.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RAB10_RAT

Accession

Primary (citable) accession number: P35281

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program