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Protein

Ras-related protein Rab-10

Gene

Rab10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.By similarity1 Publication

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the DENND4C guanine exchange factor (GEF) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 238GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi122 – 1254GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-10
Gene namesi
Name:Rab10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620879. Rab10.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • endoplasmic reticulum tubular network Source: UniProtKB
  • endosome Source: UniProtKB
  • endosome membrane Source: GO_Central
  • Golgi apparatus Source: UniProtKB
  • insulin-responsive compartment Source: UniProtKB
  • membrane Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • primary cilium Source: UniProtKB
  • recycling endosome Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Ras-related protein Rab-10PRO_0000121148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021N6-acetyllysineBy similarity
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

PRIDEiP35281.

PTM databases

PhosphoSiteiP35281.

Expressioni

Tissue specificityi

Highest levels in neural and muscle tissues.

Interactioni

Subunit structurei

Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1.1 Publication

Protein-protein interaction databases

IntActiP35281. 2 interactions.
MINTiMINT-4590846.
STRINGi10116.ENSRNOP00000065234.

Structurei

3D structure databases

ProteinModelPortaliP35281.
SMRiP35281. Positions 4-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

HOVERGENiHBG009351.
InParanoidiP35281.
PhylomeDBiP35281.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35281-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIEIDFKIKT
60 70 80 90 100
VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI
110 120 130 140 150
SKWLRNIDQH ANEDVERMLL RNKCDMDHKR VVPKGKGEQI AREHRIRFFE
160 170 180 190 200
TSAKANINIE KAFLTLPEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
Length:200
Mass (Da):22,858
Last modified:February 1, 1994 - v1
Checksum:iEFAF804FD4C3B6A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83677 mRNA. Translation: AAA41991.1.
PIRiB42148.
UniGeneiRn.65864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83677 mRNA. Translation: AAA41991.1.
PIRiB42148.
UniGeneiRn.65864.

3D structure databases

ProteinModelPortaliP35281.
SMRiP35281. Positions 4-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP35281. 2 interactions.
MINTiMINT-4590846.
STRINGi10116.ENSRNOP00000065234.

PTM databases

PhosphoSiteiP35281.

Proteomic databases

PRIDEiP35281.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi620879. Rab10.

Phylogenomic databases

HOVERGENiHBG009351.
InParanoidiP35281.
PhylomeDBiP35281.

Miscellaneous databases

PROiP35281.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells."
    Babbey C.M., Bacallao R.L., Dunn K.W.
    Am. J. Physiol. 299:F495-506(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization."
    Wang T., Liu Y., Xu X.H., Deng C.Y., Wu K.Y., Zhu J., Fu X.Q., He M., Luo Z.G.
    Dev. Cell 21:431-444(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AXON DEVELOPMENT, INTERACTION WITH LLGL1 AND GDI1.

Entry informationi

Entry nameiRAB10_RAT
AccessioniPrimary (citable) accession number: P35281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 24, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.