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P35281

- RAB10_RAT

UniProt

P35281 - RAB10_RAT

Protein

Ras-related protein Rab-10

Gene

Rab10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion By similarity. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.By similarity1 Publication

    Enzyme regulationi

    Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the DENND4C guanine exchange factor (GEF) By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 238GTPBy similarity
    Nucleotide bindingi64 – 685GTPBy similarity
    Nucleotide bindingi122 – 1254GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTP binding Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. axonogenesis Source: UniProtKB
    2. basolateral protein localization Source: UniProtKB
    3. cellular response to antibiotic Source: RGD
    4. cellular response to insulin stimulus Source: UniProtKB
    5. endoplasmic reticulum tubular network organization Source: UniProtKB
    6. endosomal transport Source: UniProtKB
    7. establishment of neuroblast polarity Source: UniProtKB
    8. establishment of protein localization to endoplasmic reticulum membrane Source: UniProtKB
    9. Golgi to plasma membrane protein transport Source: UniProtKB
    10. Golgi to plasma membrane transport Source: UniProtKB
    11. polarized epithelial cell differentiation Source: UniProtKB
    12. protein localization to plasma membrane Source: UniProtKB
    13. small GTPase mediated signal transduction Source: InterPro
    14. vesicle-mediated transport Source: UniProtKB

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-10
    Gene namesi
    Name:Rab10
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620879. Rab10.

    Subcellular locationi

    Cytoplasmic vesicle membrane By similarity; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication. Golgi apparatustrans-Golgi network membrane By similarity. Endosome membrane By similarity. Recycling endosome membrane By similarity. Cytoplasmic vesiclephagosome membrane By similarity. Cell projectioncilium 1 Publication. Endoplasmic reticulum membrane By similarity
    Note: Associates with SLC2A4/GLUT4 storage vesicles By similarity. Localizes to the base of the cilium. Transiently associates with phagosomes By similarity. Localizes to the endoplasmic reticulum at domains of new tubule growth By similarity.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB
    2. endoplasmic reticulum tubular network Source: UniProtKB
    3. endosome Source: UniProtKB
    4. Golgi apparatus Source: UniProtKB
    5. insulin-responsive compartment Source: UniProtKB
    6. membrane Source: RGD
    7. perinuclear region of cytoplasm Source: RGD
    8. phagocytic vesicle membrane Source: UniProtKB-SubCell
    9. primary cilium Source: UniProtKB
    10. recycling endosome Source: UniProtKB
    11. recycling endosome membrane Source: UniProtKB-SubCell
    12. trans-Golgi network Source: RGD

    Keywords - Cellular componenti

    Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Ras-related protein Rab-10PRO_0000121148Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei102 – 1021N6-acetyllysineBy similarity
    Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity
    Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Prenylation

    Proteomic databases

    PRIDEiP35281.

    PTM databases

    PhosphoSiteiP35281.

    Expressioni

    Tissue specificityi

    Highest levels in neural and muscle tissues.

    Gene expression databases

    GenevestigatoriP35281.

    Interactioni

    Subunit structurei

    Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1.1 Publication

    Protein-protein interaction databases

    IntActiP35281. 2 interactions.
    MINTiMINT-4590846.

    Structurei

    3D structure databases

    ProteinModelPortaliP35281.
    SMRiP35281. Positions 4-173.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 469Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    HOVERGENiHBG009351.
    PhylomeDBiP35281.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35281-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIEIDFKIKT    50
    VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI 100
    SKWLRNIDQH ANEDVERMLL RNKCDMDHKR VVPKGKGEQI AREHRIRFFE 150
    TSAKANINIE KAFLTLPEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC 200
    Length:200
    Mass (Da):22,858
    Last modified:February 1, 1994 - v1
    Checksum:iEFAF804FD4C3B6A3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83677 mRNA. Translation: AAA41991.1.
    PIRiB42148.
    UniGeneiRn.65864.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83677 mRNA. Translation: AAA41991.1 .
    PIRi B42148.
    UniGenei Rn.65864.

    3D structure databases

    ProteinModelPortali P35281.
    SMRi P35281. Positions 4-173.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35281. 2 interactions.
    MINTi MINT-4590846.

    PTM databases

    PhosphoSitei P35281.

    Proteomic databases

    PRIDEi P35281.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 620879. Rab10.

    Phylogenomic databases

    HOVERGENi HBG009351.
    PhylomeDBi P35281.

    Miscellaneous databases

    PROi P35281.

    Gene expression databases

    Genevestigatori P35281.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
      Elferink L.A., Anzai K., Scheller R.H.
      J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. "Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells."
      Babbey C.M., Bacallao R.L., Dunn K.W.
      Am. J. Physiol. 299:F495-506(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    3. "Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization."
      Wang T., Liu Y., Xu X.H., Deng C.Y., Wu K.Y., Zhu J., Fu X.Q., He M., Luo Z.G.
      Dev. Cell 21:431-444(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN AXON DEVELOPMENT, INTERACTION WITH LLGL1 AND GDI1.

    Entry informationi

    Entry nameiRAB10_RAT
    AccessioniPrimary (citable) accession number: P35281
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3