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P35281

- RAB10_RAT

UniProt

P35281 - RAB10_RAT

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Protein

Ras-related protein Rab-10

Gene

Rab10

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.By similarity1 Publication

Enzyme regulationi

Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP). That Rab is activated by the DENND4C guanine exchange factor (GEF) (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 238GTPBy similarity
Nucleotide bindingi64 – 685GTPBy similarity
Nucleotide bindingi122 – 1254GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTP binding Source: UniProtKB

GO - Biological processi

  1. axonogenesis Source: UniProtKB
  2. basolateral protein localization Source: UniProtKB
  3. cellular response to antibiotic Source: RGD
  4. cellular response to insulin stimulus Source: UniProtKB
  5. endoplasmic reticulum tubular network organization Source: UniProtKB
  6. endosomal transport Source: UniProtKB
  7. establishment of neuroblast polarity Source: UniProtKB
  8. establishment of protein localization to endoplasmic reticulum membrane Source: UniProtKB
  9. Golgi to plasma membrane protein transport Source: UniProtKB
  10. Golgi to plasma membrane transport Source: UniProtKB
  11. polarized epithelial cell differentiation Source: UniProtKB
  12. protein localization to plasma membrane Source: UniProtKB
  13. small GTPase mediated signal transduction Source: InterPro
  14. vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-10
Gene namesi
Name:Rab10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620879. Rab10.

Subcellular locationi

Cytoplasmic vesicle membrane By similarity; Lipid-anchor 1 Publication; Cytoplasmic side 1 Publication. Golgi apparatustrans-Golgi network membrane By similarity. Endosome membrane By similarity. Recycling endosome membrane By similarity. Cytoplasmic vesiclephagosome membrane By similarity. Cell projectioncilium 1 Publication. Endoplasmic reticulum membrane By similarity
Note: Associates with SLC2A4/GLUT4 storage vesicles (By similarity). Localizes to the base of the cilium. Transiently associates with phagosomes (By similarity). Localizes to the endoplasmic reticulum at domains of new tubule growth (By similarity).By similarity

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. endoplasmic reticulum tubular network Source: UniProtKB
  3. endosome Source: UniProtKB
  4. Golgi apparatus Source: UniProtKB
  5. insulin-responsive compartment Source: UniProtKB
  6. membrane Source: RGD
  7. perinuclear region of cytoplasm Source: RGD
  8. primary cilium Source: UniProtKB
  9. recycling endosome Source: UniProtKB
  10. trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Ras-related protein Rab-10PRO_0000121148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021N6-acetyllysineBy similarity
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity
Lipidationi200 – 2001S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

PRIDEiP35281.

PTM databases

PhosphoSiteiP35281.

Expressioni

Tissue specificityi

Highest levels in neural and muscle tissues.

Gene expression databases

GenevestigatoriP35281.

Interactioni

Subunit structurei

Interacts (GDP-bound form) with LLGL1; the interaction is direct and promotes RAB10 association with membranes and activation through competition with the Rab inhibitor GDI1.1 Publication

Protein-protein interaction databases

IntActiP35281. 2 interactions.
MINTiMINT-4590846.

Structurei

3D structure databases

ProteinModelPortaliP35281.
SMRiP35281. Positions 4-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 469Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

HOVERGENiHBG009351.
InParanoidiP35281.
PhylomeDBiP35281.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35281-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIEIDFKIKT
60 70 80 90 100
VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI
110 120 130 140 150
SKWLRNIDQH ANEDVERMLL RNKCDMDHKR VVPKGKGEQI AREHRIRFFE
160 170 180 190 200
TSAKANINIE KAFLTLPEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
Length:200
Mass (Da):22,858
Last modified:February 1, 1994 - v1
Checksum:iEFAF804FD4C3B6A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83677 mRNA. Translation: AAA41991.1.
PIRiB42148.
UniGeneiRn.65864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83677 mRNA. Translation: AAA41991.1 .
PIRi B42148.
UniGenei Rn.65864.

3D structure databases

ProteinModelPortali P35281.
SMRi P35281. Positions 4-173.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35281. 2 interactions.
MINTi MINT-4590846.

PTM databases

PhosphoSitei P35281.

Proteomic databases

PRIDEi P35281.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 620879. Rab10.

Phylogenomic databases

HOVERGENi HBG009351.
InParanoidi P35281.
PhylomeDBi P35281.

Miscellaneous databases

PROi P35281.

Gene expression databases

Genevestigatori P35281.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells."
    Babbey C.M., Bacallao R.L., Dunn K.W.
    Am. J. Physiol. 299:F495-506(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "Lgl1 activation of rab10 promotes axonal membrane trafficking underlying neuronal polarization."
    Wang T., Liu Y., Xu X.H., Deng C.Y., Wu K.Y., Zhu J., Fu X.Q., He M., Luo Z.G.
    Dev. Cell 21:431-444(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN AXON DEVELOPMENT, INTERACTION WITH LLGL1 AND GDI1.

Entry informationi

Entry nameiRAB10_RAT
AccessioniPrimary (citable) accession number: P35281
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3