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Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis (By similarity). Together with MICALL2, may also regulate adherens junction assembly (By similarity). May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis (PubMed:21041651). Involved in autophagy (By similarity).By similarity1 Publication

Enzyme regulationi

Activated in response to insulin.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi17 – 22GTPBy similarity6
Nucleotide bindingi34 – 40GTPBy similarity7
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi152 – 154GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • axonogenesis Source: UniProtKB
  • cellular response to insulin stimulus Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • glucose homeostasis Source: UniProtKB
  • Golgi vesicle fusion to target membrane Source: Ensembl
  • neurotransmitter receptor transport to postsynaptic membrane Source: SynGO
  • positive regulation of glucose import Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • protein secretion Source: GO_Central
  • regulation of autophagy Source: UniProtKB
  • regulation of exocytosis Source: GO_Central
  • regulation of long-term neuronal synaptic plasticity Source: RGD
  • regulation of protein localization Source: RGD
  • regulation of protein transport Source: RGD
  • vesicle docking involved in exocytosis Source: GO_Central

Keywordsi

Biological processAutophagy, Cilium biogenesis/degradation, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-5620912 Anchoring of the basal body to the plasma membrane
R-RNO-5620916 VxPx cargo-targeting to cilium
R-RNO-8854214 TBC/RABGAPs
R-RNO-8873719 RAB geranylgeranylation
R-RNO-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Gene namesi
Name:Rab8a
Synonyms:Rab8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621144 Rab8a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001211331 – 204Ras-related protein Rab-8AAdd BLAST204
PropeptideiPRO_0000370797205 – 207Removed in mature formSequence analysis3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineCombined sources1
Modified residuei185PhosphoserineBy similarity1
Modified residuei204Cysteine methyl esterSequence analysis1
Lipidationi204S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP35280
PRIDEiP35280

PTM databases

iPTMnetiP35280
PhosphoSitePlusiP35280

Expressioni

Tissue specificityi

Highest levels in spinal cord, heart, kidney and lung.

Gene expression databases

BgeeiENSRNOG00000014621
ExpressionAtlasiP35280 baseline and differential
GenevisibleiP35280 RN

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts (GTP-bound form) with MICAL1, MICALCL, MICAL3 and EHBP1L1; two molecules of RAB8A can bind to one molecule of the effector protein; ternary complexes of RAB8A, RAB13 and either MICAL1 or EHBP1L1 are possible. Interacts (GTP-bound form) with EHBP1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL. Interacts with AHI1. Interacts with DCDC5.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250675, 2 interactors
IntActiP35280, 2 interactors
STRINGi10116.ENSRNOP00000020748

Structurei

3D structure databases

ProteinModelPortaliP35280
SMRiP35280
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0078 Eukaryota
ENOG410XPUI LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiP35280
KOiK07901
OMAiNPQGSNQ
OrthoDBiEOG091G0LA6
PhylomeDBiP35280

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS

FFRCSLL
Length:207
Mass (Da):23,668
Last modified:November 22, 2005 - v2
Checksum:iAC89DC85588FB8F8
GO

Sequence cautioni

The sequence AAA41997 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102W → S in AAA41997 (PubMed:1313420).Curated1
Sequence conflicti176 – 184KLEGNSPQG → NWNPTTLR in AAA41997 (PubMed:1313420).Curated9
Sequence conflicti199T → R in AAA41997 (PubMed:1313420).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071176 mRNA Translation: AAH71176.1
BC105863 mRNA Translation: AAI05864.1
M83675 mRNA Translation: AAA41997.1 Different initiation.
PIRiA42148
RefSeqiNP_446450.2, NM_053998.2
UniGeneiRn.16064

Genome annotation databases

EnsembliENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621
GeneIDi117103
KEGGirno:117103
UCSCiRGD:621144 rat

Similar proteinsi

Entry informationi

Entry nameiRAB8A_RAT
AccessioniPrimary (citable) accession number: P35280
Secondary accession number(s): Q3B8Q4, Q6DKL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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