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Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.1 Publication

Enzyme regulationi

Activated in response to insulin.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi121 – 1244GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTPase activity Source: GO_Central
  3. GTP binding Source: UniProtKB
  4. protein kinase binding Source: RGD

GO - Biological processi

  1. axonogenesis Source: UniProtKB
  2. cellular response to insulin stimulus Source: UniProtKB
  3. cilium assembly Source: UniProtKB
  4. glucose homeostasis Source: UniProtKB
  5. Golgi vesicle fusion to target membrane Source: GO_Central
  6. intracellular protein transport Source: GO_Central
  7. metabolic process Source: GOC
  8. positive regulation of glucose import Source: UniProtKB
  9. protein localization to plasma membrane Source: UniProtKB
  10. protein secretion Source: GO_Central
  11. Rab protein signal transduction Source: GO_Central
  12. regulation of exocytosis Source: GO_Central
  13. regulation of long-term neuronal synaptic plasticity Source: RGD
  14. regulation of protein localization Source: RGD
  15. regulation of protein transport Source: RGD
  16. synaptic vesicle exocytosis Source: GO_Central
  17. vesicle docking involved in exocytosis Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_276479. VxPx cargo-targeting to cilium.
REACT_319206. Regulation of PLK1 Activity at G2/M Transition.
REACT_329529. Anchoring of the basal body to the plasma membrane.
REACT_347820. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Gene namesi
Name:Rab8a
Synonyms:Rab8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 16

Organism-specific databases

RGDi621144. Rab8a.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Golgi apparatus By similarity. Recycling endosome membrane By similarity. Cell projectioncilium By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or Mycobacterium (By similarity).By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. ciliary basal body Source: Ensembl
  3. cytoplasmic membrane-bounded vesicle Source: UniProtKB
  4. cytoplasmic vesicle membrane Source: Reactome
  5. dendrite Source: RGD
  6. endosome Source: GO_Central
  7. extracellular vesicular exosome Source: Ensembl
  8. neuronal cell body Source: RGD
  9. nonmotile primary cilium Source: Ensembl
  10. phagocytic vesicle Source: UniProtKB
  11. phagocytic vesicle membrane Source: UniProtKB-SubCell
  12. plasma membrane Source: RGD
  13. postsynaptic density Source: RGD
  14. recycling endosome membrane Source: UniProtKB
  15. secretory granule membrane Source: GO_Central
  16. synaptic vesicle Source: GO_Central
  17. trans-Golgi network transport vesicle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Ras-related protein Rab-8APRO_0000121133Add
BLAST
Propeptidei205 – 2073Removed in mature formSequence AnalysisPRO_0000370797

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphoserineBy similarity
Modified residuei185 – 1851PhosphoserineBy similarity
Modified residuei204 – 2041Cysteine methyl esterSequence Analysis
Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP35280.
PRIDEiP35280.

PTM databases

PhosphoSiteiP35280.

Expressioni

Tissue specificityi

Highest levels in spinal cord, heart, kidney and lung.

Gene expression databases

ExpressionAtlasiP35280. baseline.
GenevestigatoriP35280.

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL (By similarity).By similarity

Protein-protein interaction databases

BioGridi250675. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP35280.
SMRiP35280. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35280.
KOiK07901.
OMAiIDKAFYS.
OrthoDBiEOG7VB2H4.
PhylomeDBiP35280.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI
60 70 80 90 100
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR
110 120 130 140 150
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET
160 170 180 190 200
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS

FFRCSLL
Length:207
Mass (Da):23,668
Last modified:November 22, 2005 - v2
Checksum:iAC89DC85588FB8F8
GO

Sequence cautioni

The sequence AAA41997.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021W → S in AAA41997 (PubMed:1313420).Curated
Sequence conflicti176 – 1849KLEGNSPQG → NWNPTTLR in AAA41997 (PubMed:1313420).Curated
Sequence conflicti199 – 1991T → R in AAA41997 (PubMed:1313420).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071176 mRNA. Translation: AAH71176.1.
BC105863 mRNA. Translation: AAI05864.1.
M83675 mRNA. Translation: AAA41997.1. Different initiation.
PIRiA42148.
RefSeqiNP_446450.2. NM_053998.2.
UniGeneiRn.16064.

Genome annotation databases

EnsembliENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
GeneIDi117103.
KEGGirno:117103.
UCSCiRGD:621144. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC071176 mRNA. Translation: AAH71176.1.
BC105863 mRNA. Translation: AAI05864.1.
M83675 mRNA. Translation: AAA41997.1. Different initiation.
PIRiA42148.
RefSeqiNP_446450.2. NM_053998.2.
UniGeneiRn.16064.

3D structure databases

ProteinModelPortaliP35280.
SMRiP35280. Positions 6-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250675. 1 interaction.

PTM databases

PhosphoSiteiP35280.

Proteomic databases

PaxDbiP35280.
PRIDEiP35280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
GeneIDi117103.
KEGGirno:117103.
UCSCiRGD:621144. rat.

Organism-specific databases

CTDi4218.
RGDi621144. Rab8a.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118937.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP35280.
KOiK07901.
OMAiIDKAFYS.
OrthoDBiEOG7VB2H4.
PhylomeDBiP35280.

Enzyme and pathway databases

ReactomeiREACT_276479. VxPx cargo-targeting to cilium.
REACT_319206. Regulation of PLK1 Activity at G2/M Transition.
REACT_329529. Anchoring of the basal body to the plasma membrane.
REACT_347820. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi619992.
PROiP35280.

Gene expression databases

ExpressionAtlasiP35280. baseline.
GenevestigatoriP35280.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Testis.
  2. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-207.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
    Sun Y., Bilan P.J., Liu Z., Klip A.
    Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.

Entry informationi

Entry nameiRAB8A_RAT
AccessioniPrimary (citable) accession number: P35280
Secondary accession number(s): Q3B8Q4, Q6DKL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: April 1, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.