P35280 (RAB8A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-8A

Gene names

Name:	Rab8a
Synonyms:	Rab8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization By similarity.
Subunit structure	Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3 By similarity. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B By similarity. Interacts with PIFO By similarity. Interacts with BIRC6/bruce By similarity. Interacts with OCRL. Interacts with RABIF, RIMS2, RPH3A and RPH3A By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Golgi apparatus By similarity. Cytoplasm › perinuclear region By similarity. Cell projection By similarity. Cytoplasmic vesicle › phagosome By similarity. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.
Tissue specificity	Highest levels in spinal cord, heart, kidney and lung.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.
Sequence caution	The sequence AAA41997.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi vesicle fusion to target membrane Inferred from electronic annotation. Source: Compara axonogenesis Inferred from mutant phenotype PubMed 21856246. Source: UniProtKB cilium assembly Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of long-term neuronal synaptic plasticity Inferred from direct assay PubMed 15297461. Source: RGD regulation of protein transport Inferred from direct assay PubMed 15297461. Source: RGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro vesicle docking involved in exocytosis Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle membrane Traceable author statement. Source: Reactome microtubule basal body Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from direct assay PubMed 8408204. Source: RGD nonmotile primary cilium Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 8366094. Source: RGD postsynaptic density Inferred from direct assay PubMed 15297461. Source: RGD recycling endosome Inferred from electronic annotation. Source: Compara
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Ras-related protein Rab-8A		PRO_0000121133
	Propeptide	205 – 207	3	Removed in mature form Potential		PRO_0000370797
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	63 – 67	5	GTP By similarity
	Nucleotide binding	121 – 124	4	GTP By similarity
	Motif	37 – 45	9	Effector region By similarity
Amino acid modifications
	Modified residue	181	1	Phosphoserine By similarity
	Modified residue	185	1	Phosphoserine By similarity
	Modified residue	204	1	Cysteine methyl ester Potential
	Lipidation	204	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	102	1	W → S in AAA41997. Ref.2
	Sequence conflict	176 – 184	9	KLEGNSPQG → NWNPTTLR in AAA41997. Ref.2
	Sequence conflict	199	1	T → R in AAA41997. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35280 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: AC89DC85588FB8F8	FASTA	207	23,668
	10 20 30 40 50 60 MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI ELDGKRIKLQ 70 80 90 100 110 120 IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR NWIRNIEEHA SADVEKMILG 130 140 150 160 170 180 NKCDVNDKRQ VSKERGEKLA LDYGIKFMET SAKANINVEN AFFTLARDIK AKMDKKLEGN 190 200 SPQGSSHGVK ITVEQQKRTS FFRCSLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Testis.
[2]	"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain." Elferink L.A., Anzai K., Scheller R.H. J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-207. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC071176 mRNA. Translation: AAH71176.1. BC105863 mRNA. Translation: AAI05864.1. M83675 mRNA. Translation: AAA41997.1. Different initiation.
IPI	IPI00196789.
PIR	A42148.
RefSeq	NP_446450.2. NM_053998.2.
UniGene	Rn.16064.
3D structure databases
ProteinModelPortal	P35280.
SMR	P35280. Positions 6-176.
ModBase	Search...
PTM databases
PhosphoSite	P35280.
Proteomic databases
PaxDb	P35280.
PRIDE	P35280.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
GeneID	117103.
KEGG	rno:117103.
UCSC	RGD:621144. rat.
Organism-specific databases
CTD	4218.
RGD	621144. Rab8a.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00700000104031.
HOGENOM	HOG000233968.
HOVERGEN	HBG009351.
KO	K07901.
OrthoDB	EOG4K3KX6.
Enzyme and pathway databases
Reactome	REACT_97881. Membrane Trafficking.
Gene expression databases
ArrayExpress	P35280.
Genevestigator	P35280.
GermOnline	ENSRNOG00000014621. Rattus norvegicus.
Family and domain databases
InterPro	IPR027417. P-loop_NTPase. IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR003579. Small_GTPase_Rab_type. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619992.

Entry information

Entry name

RAB8A_RAT

Accession

Primary (citable) accession number: P35280
Secondary accession number(s): Q3B8Q4, Q6DKL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 22, 2005
Last modified:	May 29, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program