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P35280 (RAB8A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-8A
Gene names
Name:Rab8a
Synonyms:Rab8
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Ref.3

Enzyme regulation

Activated in response to insulin. Ref.3

Subunit structure

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL By similarity.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side Potential. Golgi apparatus By similarity. Recycling endosome membrane By similarity. Cell projectioncilium By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.

Tissue specificity

Highest levels in spinal cord, heart, kidney and lung.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence AAA41997.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCilium biogenesis/degradation
Protein transport
Transport
   Cellular componentCell membrane
Cell projection
Cytoplasmic vesicle
Endosome
Golgi apparatus
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi vesicle fusion to target membrane

Inferred from electronic annotation. Source: Ensembl

axonogenesis

Inferred from mutant phenotype PubMed 21856246. Source: UniProtKB

cellular response to insulin stimulus

Inferred from mutant phenotype Ref.3. Source: UniProtKB

cilium assembly

Inferred from sequence or structural similarity. Source: UniProtKB

glucose homeostasis

Inferred by curator Ref.3. Source: UniProtKB

positive regulation of glucose import

Inferred by curator Ref.3. Source: UniProtKB

protein localization to plasma membrane

Inferred from direct assay Ref.3. Source: UniProtKB

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of long-term neuronal synaptic plasticity

Inferred from direct assay PubMed 15297461. Source: RGD

regulation of protein localization

Inferred from mutant phenotype PubMed 18701652. Source: RGD

regulation of protein transport

Inferred from direct assay PubMed 15297461. Source: RGD

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

vesicle docking involved in exocytosis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic membrane-bounded vesicle

Inferred from direct assay PubMed 15837803. Source: UniProtKB

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

dendrite

Inferred from direct assay PubMed 8408204. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 8408204. Source: RGD

nonmotile primary cilium

Inferred from electronic annotation. Source: Ensembl

phagocytic vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay PubMed 8366094. Source: RGD

postsynaptic density

Inferred from direct assay PubMed 15297461. Source: RGD

recycling endosome membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTPase activity

Inferred from electronic annotation. Source: Ensembl

protein kinase binding

Inferred from physical interaction PubMed 12700184. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 204204Ras-related protein Rab-8A
PRO_0000121133
Propeptide205 – 2073Removed in mature form Potential
PRO_0000370797

Regions

Nucleotide binding15 – 228GTP By similarity
Nucleotide binding63 – 675GTP By similarity
Nucleotide binding121 – 1244GTP By similarity
Motif37 – 459Effector region By similarity

Amino acid modifications

Modified residue1811Phosphoserine By similarity
Modified residue1851Phosphoserine By similarity
Modified residue2041Cysteine methyl ester Potential
Lipidation2041S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict1021W → S in AAA41997. Ref.2
Sequence conflict176 – 1849KLEGNSPQG → NWNPTTLR in AAA41997. Ref.2
Sequence conflict1991T → R in AAA41997. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35280 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: AC89DC85588FB8F8

FASTA20723,668
        10         20         30         40         50         60 
MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI ELDGKRIKLQ 

        70         80         90        100        110        120 
IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR NWIRNIEEHA SADVEKMILG 

       130        140        150        160        170        180 
NKCDVNDKRQ VSKERGEKLA LDYGIKFMET SAKANINVEN AFFTLARDIK AKMDKKLEGN 

       190        200 
SPQGSSHGVK ITVEQQKRTS FFRCSLL 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung and Testis.
[2]"Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
Elferink L.A., Anzai K., Scheller R.H.
J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-207.
Strain: Sprague-Dawley.
Tissue: Brain.
[3]"Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
Sun Y., Bilan P.J., Liu Z., Klip A.
Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC071176 mRNA. Translation: AAH71176.1.
BC105863 mRNA. Translation: AAI05864.1.
M83675 mRNA. Translation: AAA41997.1. Different initiation.
PIRA42148.
RefSeqNP_446450.2. NM_053998.2.
UniGeneRn.16064.

3D structure databases

ProteinModelPortalP35280.
SMRP35280. Positions 6-176.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP35280.

Proteomic databases

PaxDbP35280.
PRIDEP35280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
GeneID117103.
KEGGrno:117103.
UCSCRGD:621144. rat.

Organism-specific databases

CTD4218.
RGD621144. Rab8a.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00750000117584.
HOGENOMHOG000233968.
HOVERGENHBG009351.
KOK07901.
OMAIDKAFYS.
OrthoDBEOG7VB2H4.
PhylomeDBP35280.

Gene expression databases

GenevestigatorP35280.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619992.
PROP35280.

Entry information

Entry nameRAB8A_RAT
AccessionPrimary (citable) accession number: P35280
Secondary accession number(s): Q3B8Q4, Q6DKL2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families