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P35280

- RAB8A_RAT

UniProt

P35280 - RAB8A_RAT

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Protein
Ras-related protein Rab-8A
Gene
Rab8a, Rab8
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.1 Publication

Enzyme regulationi

Activated in response to insulin.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTP By similarity
Nucleotide bindingi63 – 675GTP By similarity
Nucleotide bindingi121 – 1244GTP By similarity

GO - Molecular functioni

  1. GDP binding Source: UniProtKB
  2. GTP binding Source: UniProtKB
  3. GTPase activity Source: Ensembl
  4. protein kinase binding Source: RGD

GO - Biological processi

  1. Golgi vesicle fusion to target membrane Source: Ensembl
  2. axonogenesis Source: UniProtKB
  3. cellular response to insulin stimulus Source: UniProtKB
  4. cilium assembly Source: UniProtKB
  5. glucose homeostasis Source: UniProtKB
  6. positive regulation of glucose import Source: UniProtKB
  7. protein localization to plasma membrane Source: UniProtKB
  8. protein transport Source: UniProtKB-KW
  9. regulation of long-term neuronal synaptic plasticity Source: RGD
  10. regulation of protein localization Source: RGD
  11. regulation of protein transport Source: RGD
  12. small GTPase mediated signal transduction Source: InterPro
  13. vesicle docking involved in exocytosis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198294. Regulation of PLK1 Activity at G2/M Transition.
REACT_199176. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-8A
Gene namesi
Name:Rab8a
Synonyms:Rab8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 16

Organism-specific databases

RGDi621144. Rab8a.

Subcellular locationi

Cell membrane; Lipid-anchor; Cytoplasmic side Reviewed prediction. Golgi apparatus By similarity. Recycling endosome membrane By similarity. Cell projectioncilium By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity
Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. cytoplasmic membrane-bounded vesicle Source: UniProtKB
  3. cytoplasmic vesicle membrane Source: Reactome
  4. dendrite Source: RGD
  5. neuronal cell body Source: RGD
  6. nonmotile primary cilium Source: Ensembl
  7. phagocytic vesicle Source: UniProtKB
  8. phagocytic vesicle membrane Source: UniProtKB-SubCell
  9. plasma membrane Source: RGD
  10. postsynaptic density Source: RGD
  11. recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Ras-related protein Rab-8A
PRO_0000121133Add
BLAST
Propeptidei205 – 2073Removed in mature form Reviewed prediction
PRO_0000370797

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Phosphoserine By similarity
Modified residuei185 – 1851Phosphoserine By similarity
Modified residuei204 – 2041Cysteine methyl ester Reviewed prediction
Lipidationi204 – 2041S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP35280.
PRIDEiP35280.

PTM databases

PhosphoSiteiP35280.

Expressioni

Tissue specificityi

Highest levels in spinal cord, heart, kidney and lung.

Gene expression databases

GenevestigatoriP35280.

Interactioni

Subunit structurei

Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL By similarity.

Protein-protein interaction databases

BioGridi250675. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP35280.
SMRiP35280. Positions 6-176.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector region By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00750000117584.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
KOiK07901.
OMAiIDKAFYS.
OrthoDBiEOG7VB2H4.
PhylomeDBiP35280.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35280-1 [UniParc]FASTAAdd to Basket

« Hide

MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI    50
ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR 100
NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET 150
SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS 200
FFRCSLL 207
Length:207
Mass (Da):23,668
Last modified:November 22, 2005 - v2
Checksum:iAC89DC85588FB8F8
GO

Sequence cautioni

The sequence AAA41997.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021W → S in AAA41997. 1 Publication
Sequence conflicti176 – 1849KLEGNSPQG → NWNPTTLR in AAA41997. 1 Publication
Sequence conflicti199 – 1991T → R in AAA41997. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC071176 mRNA. Translation: AAH71176.1.
BC105863 mRNA. Translation: AAI05864.1.
M83675 mRNA. Translation: AAA41997.1. Different initiation.
PIRiA42148.
RefSeqiNP_446450.2. NM_053998.2.
UniGeneiRn.16064.

Genome annotation databases

EnsembliENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
GeneIDi117103.
KEGGirno:117103.
UCSCiRGD:621144. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC071176 mRNA. Translation: AAH71176.1 .
BC105863 mRNA. Translation: AAI05864.1 .
M83675 mRNA. Translation: AAA41997.1 . Different initiation.
PIRi A42148.
RefSeqi NP_446450.2. NM_053998.2.
UniGenei Rn.16064.

3D structure databases

ProteinModelPortali P35280.
SMRi P35280. Positions 6-176.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250675. 1 interaction.

PTM databases

PhosphoSitei P35280.

Proteomic databases

PaxDbi P35280.
PRIDEi P35280.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000020748 ; ENSRNOP00000020748 ; ENSRNOG00000014621 .
GeneIDi 117103.
KEGGi rno:117103.
UCSCi RGD:621144. rat.

Organism-specific databases

CTDi 4218.
RGDi 621144. Rab8a.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00750000117584.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
KOi K07901.
OMAi IDKAFYS.
OrthoDBi EOG7VB2H4.
PhylomeDBi P35280.

Enzyme and pathway databases

Reactomei REACT_198294. Regulation of PLK1 Activity at G2/M Transition.
REACT_199176. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

NextBioi 619992.
PROi P35280.

Gene expression databases

Genevestigatori P35280.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung and Testis.
  2. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
    Elferink L.A., Anzai K., Scheller R.H.
    J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-207.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
    Sun Y., Bilan P.J., Liu Z., Klip A.
    Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.

Entry informationi

Entry nameiRAB8A_RAT
AccessioniPrimary (citable) accession number: P35280
Secondary accession number(s): Q3B8Q4, Q6DKL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: November 22, 2005
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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