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P35280

- RAB8A_RAT

UniProt

P35280 - RAB8A_RAT

Protein

Ras-related protein Rab-8A

Gene

Rab8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.1 Publication

    Enzyme regulationi

    Activated in response to insulin.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 228GTPBy similarity
    Nucleotide bindingi63 – 675GTPBy similarity
    Nucleotide bindingi121 – 1244GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTPase activity Source: Ensembl
    3. GTP binding Source: UniProtKB
    4. protein kinase binding Source: RGD

    GO - Biological processi

    1. axonogenesis Source: UniProtKB
    2. cellular response to insulin stimulus Source: UniProtKB
    3. cilium assembly Source: UniProtKB
    4. glucose homeostasis Source: UniProtKB
    5. Golgi vesicle fusion to target membrane Source: Ensembl
    6. positive regulation of glucose import Source: UniProtKB
    7. protein localization to plasma membrane Source: UniProtKB
    8. protein transport Source: UniProtKB-KW
    9. regulation of long-term neuronal synaptic plasticity Source: RGD
    10. regulation of protein localization Source: RGD
    11. regulation of protein transport Source: RGD
    12. small GTPase mediated signal transduction Source: InterPro
    13. vesicle docking involved in exocytosis Source: Ensembl

    Keywords - Biological processi

    Cilium biogenesis/degradation, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198294. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199176. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-8A
    Gene namesi
    Name:Rab8a
    Synonyms:Rab8
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 16

    Organism-specific databases

    RGDi621144. Rab8a.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Golgi apparatus By similarity. Recycling endosome membrane By similarity. Cell projectioncilium By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
    Note: Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and lumenogenesis. Localizes to tubular recycling endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB
    2. cytoplasmic vesicle membrane Source: Reactome
    3. dendrite Source: RGD
    4. Golgi apparatus Source: UniProtKB-SubCell
    5. neuronal cell body Source: RGD
    6. nonmotile primary cilium Source: Ensembl
    7. phagocytic vesicle Source: UniProtKB
    8. phagocytic vesicle membrane Source: UniProtKB-SubCell
    9. plasma membrane Source: RGD
    10. postsynaptic density Source: RGD
    11. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 204204Ras-related protein Rab-8APRO_0000121133Add
    BLAST
    Propeptidei205 – 2073Removed in mature formSequence AnalysisPRO_0000370797

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei181 – 1811PhosphoserineBy similarity
    Modified residuei185 – 1851PhosphoserineBy similarity
    Modified residuei204 – 2041Cysteine methyl esterSequence Analysis
    Lipidationi204 – 2041S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiP35280.
    PRIDEiP35280.

    PTM databases

    PhosphoSiteiP35280.

    Expressioni

    Tissue specificityi

    Highest levels in spinal cord, heart, kidney and lung.

    Gene expression databases

    GenevestigatoriP35280.

    Interactioni

    Subunit structurei

    Interacts (GTP-bound form) with MICALL1; regulates RAB8A association with recycling endosomes. Interacts with MICALL2; competes with RAB13 and is involved in E-cadherin endocytic recycling. Interacts with MICAL1. Interacts with EHD1. Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3. Interacts with RABIF, RIMS2, RPH3A and RPH3A. Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL By similarity.By similarity

    Protein-protein interaction databases

    BioGridi250675. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP35280.
    SMRiP35280. Positions 6-176.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi37 – 459Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00750000117584.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    KOiK07901.
    OMAiIDKAFYS.
    OrthoDBiEOG7VB2H4.
    PhylomeDBiP35280.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35280-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKTYDYLFK LLLIGDSGVG KTCVLFRFSE DAFNSTFIST IGIDFKIRTI    50
    ELDGKRIKLQ IWDTAGQERF RTITTAYYRG AMGIMLVYDI TNEKSFDNIR 100
    NWIRNIEEHA SADVEKMILG NKCDVNDKRQ VSKERGEKLA LDYGIKFMET 150
    SAKANINVEN AFFTLARDIK AKMDKKLEGN SPQGSSHGVK ITVEQQKRTS 200
    FFRCSLL 207
    Length:207
    Mass (Da):23,668
    Last modified:November 22, 2005 - v2
    Checksum:iAC89DC85588FB8F8
    GO

    Sequence cautioni

    The sequence AAA41997.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021W → S in AAA41997. (PubMed:1313420)Curated
    Sequence conflicti176 – 1849KLEGNSPQG → NWNPTTLR in AAA41997. (PubMed:1313420)Curated
    Sequence conflicti199 – 1991T → R in AAA41997. (PubMed:1313420)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC071176 mRNA. Translation: AAH71176.1.
    BC105863 mRNA. Translation: AAI05864.1.
    M83675 mRNA. Translation: AAA41997.1. Different initiation.
    PIRiA42148.
    RefSeqiNP_446450.2. NM_053998.2.
    UniGeneiRn.16064.

    Genome annotation databases

    EnsembliENSRNOT00000020748; ENSRNOP00000020748; ENSRNOG00000014621.
    GeneIDi117103.
    KEGGirno:117103.
    UCSCiRGD:621144. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC071176 mRNA. Translation: AAH71176.1 .
    BC105863 mRNA. Translation: AAI05864.1 .
    M83675 mRNA. Translation: AAA41997.1 . Different initiation.
    PIRi A42148.
    RefSeqi NP_446450.2. NM_053998.2.
    UniGenei Rn.16064.

    3D structure databases

    ProteinModelPortali P35280.
    SMRi P35280. Positions 6-176.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250675. 1 interaction.

    PTM databases

    PhosphoSitei P35280.

    Proteomic databases

    PaxDbi P35280.
    PRIDEi P35280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000020748 ; ENSRNOP00000020748 ; ENSRNOG00000014621 .
    GeneIDi 117103.
    KEGGi rno:117103.
    UCSCi RGD:621144. rat.

    Organism-specific databases

    CTDi 4218.
    RGDi 621144. Rab8a.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00750000117584.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    KOi K07901.
    OMAi IDKAFYS.
    OrthoDBi EOG7VB2H4.
    PhylomeDBi P35280.

    Enzyme and pathway databases

    Reactomei REACT_198294. Regulation of PLK1 Activity at G2/M Transition.
    REACT_199176. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    NextBioi 619992.
    PROi P35280.

    Gene expression databases

    Genevestigatori P35280.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung and Testis.
    2. "Rab15, a novel low molecular weight GTP-binding protein specifically expressed in rat brain."
      Elferink L.A., Anzai K., Scheller R.H.
      J. Biol. Chem. 267:5768-5775(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-207.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    3. "Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells."
      Sun Y., Bilan P.J., Liu Z., Klip A.
      Proc. Natl. Acad. Sci. U.S.A. 107:19909-19914(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN EXOCYTOSIS, ENZYME REGULATION.

    Entry informationi

    Entry nameiRAB8A_RAT
    AccessioniPrimary (citable) accession number: P35280
    Secondary accession number(s): Q3B8Q4, Q6DKL2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3