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Protein

Matrix protein VP40

Gene

VP40

Organism
Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. May play a role in genome replication (By similarity).By similarity

Miscellaneous

Most abundant protein in the virion.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host JAK1 by virus, Viral budding, Viral budding via the host ESCRT complexes, Viral immunoevasion, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene namesi
Name:VP40
OrganismiLake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Taxonomic identifieri33727 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeMarburgvirus
Virus hostiChlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
Proteomesi
  • UP000137266 Componenti: Genome
  • UP000007771 Componenti: Genome
  • UP000160614 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16P → A: 30% loss of virus-like particles. 1 Publication1
Mutagenesisi17P → A: 50% loss of virus-like particles. 1 Publication1
Mutagenesisi19Y → A: 70% loss of virus-like particles. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002221651 – 303Matrix protein VP40Add BLAST303

Interactioni

Subunit structurei

Interacts with host TSG101.1 Publication

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 45Combined sources4
Helixi49 – 51Combined sources3
Beta strandi58 – 72Combined sources15
Beta strandi77 – 90Combined sources14
Helixi91 – 93Combined sources3
Helixi96 – 105Combined sources10
Beta strandi108 – 117Combined sources10
Beta strandi119 – 125Combined sources7
Helixi136 – 140Combined sources5
Beta strandi141 – 146Combined sources6
Helixi147 – 149Combined sources3
Beta strandi161 – 173Combined sources13
Helixi183 – 187Combined sources5
Beta strandi191 – 198Combined sources8
Helixi211 – 214Combined sources4
Turni220 – 222Combined sources3
Helixi223 – 229Combined sources7
Helixi230 – 234Combined sources5
Beta strandi237 – 239Combined sources3
Beta strandi243 – 250Combined sources8
Turni253 – 255Combined sources3
Beta strandi259 – 261Combined sources3
Beta strandi272 – 276Combined sources5
Helixi283 – 285Combined sources3
Beta strandi290 – 296Combined sources7
Helixi298 – 300Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5B0VX-ray2.81A/B2-303[»]
ProteinModelPortaliP35260.
SMRiP35260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi16 – 19PPXY motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p19 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase (Potential).Curated

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000FQ.

Family and domain databases

InterProiView protein in InterPro
IPR008986. EV_matrix.
PfamiView protein in Pfam
PF07447. VP40. 1 hit.
PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMiSSF50012. SSF50012. 1 hit.

Sequencei

Sequence statusi: Complete.

P35260-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSNYNTY MQYLNPPPYA DHGANQLIPA DQLSNQQGIT PNYVGDLNLD
60 70 80 90 100
DQFKGNVCHA FTLEAIIDIS AYNERTVKGV PAWLPLGIMS NFEYPLAHTV
110 120 130 140 150
AALLTGSYTI TQFTHNGQKF VRVNRLGTGI PAHPLRMLRE GNQAFIQNMV
160 170 180 190 200
IPRNFSTNQF TYNLTNLVLS VQKLPDDAWR PSKDKLIGNT MHPAVSIHPN
210 220 230 240 250
LPPIVLPTVK KQAYRQHKNP NNGPLLAISG ILHQLRVEKV PEKTSLFRIS
260 270 280 290 300
LPADMFSVKE GMMKKRGENS PVVYFQAPEN FPLNGFNNRQ VVLAYANPTL

SAV
Length:303
Mass (Da):33,794
Last modified:January 15, 2008 - v3
Checksum:i00DA12CE6BB4A354
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti16P → S in strain: Isolate Feldmann. 1
Natural varianti184D → N in strain: pp3/guinea pig lethal. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12132 mRNA. Translation: CAA78116.1.
AY430365 Genomic RNA. Translation: AAR85462.1.
AY430366 Genomic RNA. Translation: AAR85455.1.
DQ217792 Genomic RNA. Translation: ABA87126.1.
RefSeqiYP_001531155.1. NC_001608.3.

Genome annotation databases

GeneIDi920947.
KEGGivg:920947.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVP40_MABVM
AccessioniPrimary (citable) accession number: P35260
Secondary accession number(s): Q38L43, Q6T6U1, Q6T6U8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 15, 2008
Last modified: February 15, 2017
This is version 74 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families