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Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Viral RNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase cofactor VP35
Gene namesi
Name:VP35
OrganismiLake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Taxonomic identifieri33727 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeMarburgvirus
Virus hostiChlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
Proteomesi
  • UP000007771 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral nucleocapsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90L → A: Complete loss of homo-oligomerization; when associated with A-104. 1 Publication1
Mutagenesisi104L → A: Complete loss of homo-oligomerization; when associated with A-90. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002221621 – 329Polymerase cofactor VP35Add BLAST329

Interactioni

Subunit structurei

Homooligomer. Homomultimerization via the coiled coil domain is a prerequisite for binding to L. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein.1 Publication

Protein-protein interaction databases

DIPiDIP-60094N.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi210 – 218Combined sources9
Beta strandi222 – 225Combined sources4
Helixi227 – 241Combined sources15
Helixi245 – 257Combined sources13
Helixi262 – 272Combined sources11
Helixi274 – 276Combined sources3
Beta strandi283 – 285Combined sources3
Helixi289 – 291Combined sources3
Helixi294 – 299Combined sources6
Helixi309 – 311Combined sources3
Beta strandi313 – 318Combined sources6
Beta strandi324 – 328Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GH9X-ray1.65A204-329[»]
4GHAX-ray2.50A/C/E/G204-329[»]
SMRiP35259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini204 – 329VP35 IIDPROSITE-ProRule annotationAdd BLAST126

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili70 – 120Sequence analysisAdd BLAST51

Sequence similaritiesi

Belongs to the filoviridae polymerase cofactor VP35 family.PROSITE-ProRule annotationCurated
Contains 1 VP35 IID (interferon inhibitory) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002953. Filo_VP35.
IPR031163. VP35_IID.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.
PROSITEiPS51735. VP35_IID. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC
60 70 80 90 100
LMSKATSTDD IIWDQLIVKR TLADLLIPIN RQISDIQSTL SEVTTRVHEI
110 120 130 140 150
ERQLHEITPV LKMGRTLEAI SKGMSEMLAK YDHLVISTGR TTAPAAAFDA
160 170 180 190 200
YLNEHGVPPP QPAIFKDLGV AQQACSKGTM VKNATTDAAD KMSKVLELSE
210 220 230 240 250
ETFSKPNLSA KDLALLLFTH LPGNNTPFHI LAQVLSKIAY KSGKSGAFLD
260 270 280 290 300
AFHQILSEGE NAQAALTRLS RTFDAFLGVV PPVIRVKNFQ TVPRPSQKSL
310 320
RAVPPNPTID KGWVCVYSSE QGETRALKI
Length:329
Mass (Da):36,143
Last modified:February 1, 1994 - v1
Checksum:i300EDE165A40938E
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti31E → K in strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal. 1
Natural varianti296S → C in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12132 mRNA. Translation: CAA78115.1.
AY430365 Genomic RNA. Translation: AAR85461.1.
AY430366 Genomic RNA. Translation: AAR85454.1.
DQ217792 Genomic RNA. Translation: ABA87125.1.
RefSeqiYP_001531154.1. NC_001608.3.

Genome annotation databases

GeneIDi920948.
KEGGivg:920948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12132 mRNA. Translation: CAA78115.1.
AY430365 Genomic RNA. Translation: AAR85461.1.
AY430366 Genomic RNA. Translation: AAR85454.1.
DQ217792 Genomic RNA. Translation: ABA87125.1.
RefSeqiYP_001531154.1. NC_001608.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GH9X-ray1.65A204-329[»]
4GHAX-ray2.50A/C/E/G204-329[»]
SMRiP35259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60094N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi920948.
KEGGivg:920948.

Family and domain databases

InterProiIPR002953. Filo_VP35.
IPR031163. VP35_IID.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.
PROSITEiPS51735. VP35_IID. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVP35_MABVM
AccessioniPrimary (citable) accession number: P35259
Secondary accession number(s): Q38L44, Q6T6U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.