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P35259

- VP35_MABVM

UniProt

P35259 - VP35_MABVM

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Protein
Polymerase cofactor VP35
Gene
VP35
Organism
Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex.

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Viral RNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase cofactor VP35
Gene namesi
Name:VP35
OrganismiLake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Taxonomic identifieri33727 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeMarburgvirus
Virus hostiChlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]
Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
ProteomesiUP000007771: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. inclusion body Source: CACAO
  3. viral nucleocapsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 901L → A: Complete loss of homo-oligomerization; when associated with A-104. 1 Publication
Mutagenesisi104 – 1041L → A: Complete loss of homo-oligomerization; when associated with A-90. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Polymerase cofactor VP35
PRO_0000222162Add
BLAST

Interactioni

Subunit structurei

Homooligomer. Homomultimerization via the coiled coil domain is a prerequisite for binding to L. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein.1 Publication

Protein-protein interaction databases

DIPiDIP-60094N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi210 – 2189
Beta strandi222 – 2254
Helixi227 – 24115
Helixi245 – 25713
Helixi262 – 27211
Helixi274 – 2763
Beta strandi283 – 2853
Helixi289 – 2913
Helixi294 – 2996
Helixi309 – 3113
Beta strandi313 – 3186
Beta strandi324 – 3285

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GH9X-ray1.65A204-329[»]
4GHAX-ray2.50A/C/E/G204-329[»]

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili70 – 12051 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002953. Filo_VP35.
[Graphical view]
PfamiPF02097. Filo_VP35. 1 hit.
[Graphical view]
PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
PRINTSiPR01240. FILOVP35.

Sequencei

Sequence statusi: Complete.

P35259-1 [UniParc]FASTAAdd to Basket

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MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC    50
LMSKATSTDD IIWDQLIVKR TLADLLIPIN RQISDIQSTL SEVTTRVHEI 100
ERQLHEITPV LKMGRTLEAI SKGMSEMLAK YDHLVISTGR TTAPAAAFDA 150
YLNEHGVPPP QPAIFKDLGV AQQACSKGTM VKNATTDAAD KMSKVLELSE 200
ETFSKPNLSA KDLALLLFTH LPGNNTPFHI LAQVLSKIAY KSGKSGAFLD 250
AFHQILSEGE NAQAALTRLS RTFDAFLGVV PPVIRVKNFQ TVPRPSQKSL 300
RAVPPNPTID KGWVCVYSSE QGETRALKI 329
Length:329
Mass (Da):36,143
Last modified:February 1, 1994 - v1
Checksum:i300EDE165A40938E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311E → K in strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
Natural varianti296 – 2961S → C in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12132 mRNA. Translation: CAA78115.1.
AY430365 Genomic RNA. Translation: AAR85461.1.
AY430366 Genomic RNA. Translation: AAR85454.1.
DQ217792 Genomic RNA. Translation: ABA87125.1.
RefSeqiYP_001531154.1. NC_001608.3.

Genome annotation databases

GeneIDi920948.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12132 mRNA. Translation: CAA78115.1 .
AY430365 Genomic RNA. Translation: AAR85461.1 .
AY430366 Genomic RNA. Translation: AAR85454.1 .
DQ217792 Genomic RNA. Translation: ABA87125.1 .
RefSeqi YP_001531154.1. NC_001608.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GH9 X-ray 1.65 A 204-329 [» ]
4GHA X-ray 2.50 A/C/E/G 204-329 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60094N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 920948.

Family and domain databases

InterProi IPR002953. Filo_VP35.
[Graphical view ]
Pfami PF02097. Filo_VP35. 1 hit.
[Graphical view ]
PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
PRINTSi PR01240. FILOVP35.
ProtoNeti Search...

Publicationsi

  1. "Marburg virus, a filovirus: messenger RNAs, gene order, and regulatory elements of the replication cycle."
    Feldmann H., Muehlberger E., Randolf A., Will C., Kiley M.P., Sanchez A., Klenk H.-D.
    Virus Res. 24:1-19(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Chain P.S.G., Malfatti S.A., Hajjaj A., Vergez L.M., Do L.H., Smith K.L., McCready P.M.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
  3. Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
  4. "Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30."
    Enterlein S., Volchkov V., Weik M., Kolesnikova L., Volchkova V., Klenk H.-D., Muehlberger E.
    J. Virol. 80:1038-1043(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate Enterlein.
  5. "Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcription."
    Moeller P., Pariente N., Klenk H.-D., Becker S.
    J. Virol. 79:14876-14886(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, COILED-COIL DOMAIN, MUTAGENESIS OF LEU-90 AND LEU-104.
  6. "Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein."
    Dicarlo A., Moeller P., Lander A., Kolesnikova L., Becker S.
    Virol. J. 4:105-105(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE NUCLEOPROTEIN, COILED-COIL DOMAIN.

Entry informationi

Entry nameiVP35_MABVM
AccessioniPrimary (citable) accession number: P35259
Secondary accession number(s): Q38L44, Q6T6U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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