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P35259

- VP35_MABVM

UniProt

P35259 - VP35_MABVM

Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex.

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Viral RNA replication

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase cofactor VP35
    Gene namesi
    Name:VP35
    OrganismiLake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))
    Taxonomic identifieri33727 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeMarburgvirus
    Virus hostiChlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
    Homo sapiens (Human) [TaxID: 9606]
    Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
    ProteomesiUP000007771: Genome

    Subcellular locationi

    Virion. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. inclusion body Source: CACAO
    3. viral nucleocapsid Source: CACAO

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi90 – 901L → A: Complete loss of homo-oligomerization; when associated with A-104. 1 Publication
    Mutagenesisi104 – 1041L → A: Complete loss of homo-oligomerization; when associated with A-90. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329Polymerase cofactor VP35PRO_0000222162Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer. Homomultimerization via the coiled coil domain is a prerequisite for binding to L. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein.1 Publication

    Protein-protein interaction databases

    DIPiDIP-60094N.

    Structurei

    Secondary structure

    1
    329
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi210 – 2189
    Beta strandi222 – 2254
    Helixi227 – 24115
    Helixi245 – 25713
    Helixi262 – 27211
    Helixi274 – 2763
    Beta strandi283 – 2853
    Helixi289 – 2913
    Helixi294 – 2996
    Helixi309 – 3113
    Beta strandi313 – 3186
    Beta strandi324 – 3285

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GH9X-ray1.65A204-329[»]
    4GHAX-ray2.50A/C/E/G204-329[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili70 – 12051Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002953. Filo_VP35.
    [Graphical view]
    PfamiPF02097. Filo_VP35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
    PRINTSiPR01240. FILOVP35.

    Sequencei

    Sequence statusi: Complete.

    P35259-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC    50
    LMSKATSTDD IIWDQLIVKR TLADLLIPIN RQISDIQSTL SEVTTRVHEI 100
    ERQLHEITPV LKMGRTLEAI SKGMSEMLAK YDHLVISTGR TTAPAAAFDA 150
    YLNEHGVPPP QPAIFKDLGV AQQACSKGTM VKNATTDAAD KMSKVLELSE 200
    ETFSKPNLSA KDLALLLFTH LPGNNTPFHI LAQVLSKIAY KSGKSGAFLD 250
    AFHQILSEGE NAQAALTRLS RTFDAFLGVV PPVIRVKNFQ TVPRPSQKSL 300
    RAVPPNPTID KGWVCVYSSE QGETRALKI 329
    Length:329
    Mass (Da):36,143
    Last modified:February 1, 1994 - v1
    Checksum:i300EDE165A40938E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311E → K in strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
    Natural varianti296 – 2961S → C in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12132 mRNA. Translation: CAA78115.1.
    AY430365 Genomic RNA. Translation: AAR85461.1.
    AY430366 Genomic RNA. Translation: AAR85454.1.
    DQ217792 Genomic RNA. Translation: ABA87125.1.
    RefSeqiYP_001531154.1. NC_001608.3.

    Genome annotation databases

    GeneIDi920948.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12132 mRNA. Translation: CAA78115.1 .
    AY430365 Genomic RNA. Translation: AAR85461.1 .
    AY430366 Genomic RNA. Translation: AAR85454.1 .
    DQ217792 Genomic RNA. Translation: ABA87125.1 .
    RefSeqi YP_001531154.1. NC_001608.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GH9 X-ray 1.65 A 204-329 [» ]
    4GHA X-ray 2.50 A/C/E/G 204-329 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60094N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 920948.

    Family and domain databases

    InterProi IPR002953. Filo_VP35.
    [Graphical view ]
    Pfami PF02097. Filo_VP35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
    PRINTSi PR01240. FILOVP35.
    ProtoNeti Search...

    Publicationsi

    1. "Marburg virus, a filovirus: messenger RNAs, gene order, and regulatory elements of the replication cycle."
      Feldmann H., Muehlberger E., Randolf A., Will C., Kiley M.P., Sanchez A., Klenk H.-D.
      Virus Res. 24:1-19(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Chain P.S.G., Malfatti S.A., Hajjaj A., Vergez L.M., Do L.H., Smith K.L., McCready P.M.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
    3. Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A.
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
    4. "Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30."
      Enterlein S., Volchkov V., Weik M., Kolesnikova L., Volchkova V., Klenk H.-D., Muehlberger E.
      J. Virol. 80:1038-1043(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate Enterlein.
    5. "Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcription."
      Moeller P., Pariente N., Klenk H.-D., Becker S.
      J. Virol. 79:14876-14886(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, COILED-COIL DOMAIN, MUTAGENESIS OF LEU-90 AND LEU-104.
    6. "Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein."
      Dicarlo A., Moeller P., Lander A., Kolesnikova L., Becker S.
      Virol. J. 4:105-105(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE NUCLEOPROTEIN, COILED-COIL DOMAIN.

    Entry informationi

    Entry nameiVP35_MABVM
    AccessioniPrimary (citable) accession number: P35259
    Secondary accession number(s): Q38L44, Q6T6U2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3