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P35246 (SFTPD_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pulmonary surfactant-associated protein D

Short name=PSP-D
Short name=SP-D
Alternative name(s):
Lung surfactant protein D
Gene names
Name:SFTPD
Synonyms:SFTP4
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Subunit structure

Oligomeric complex of 4 set of homotrimers.

Subcellular location

Secretedextracellular spaceextracellular matrix. Secretedextracellular spacesurface film.

Post-translational modification

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates By similarity.

S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages By similarity.

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Sequence similarities

Belongs to the SFTPD family.

Contains 1 C-type lectin domain.

Contains 1 collagen-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Chain21 – 369349Pulmonary surfactant-associated protein D
PRO_0000017464

Regions

Domain46 – 216171Collagen-like
Domain254 – 369116C-type lectin
Coiled coil217 – 24832 Potential

Amino acid modifications

Modified residue351S-nitrosocysteine By similarity
Modified residue401S-nitrosocysteine By similarity
Modified residue7814-hydroxyproline By similarity
Modified residue8715-hydroxylysine By similarity
Modified residue9614-hydroxyproline By similarity
Modified residue9915-hydroxylysine By similarity
Modified residue16514-hydroxyproline By similarity
Modified residue17114-hydroxyproline By similarity
Glycosylation901N-linked (GlcNAc...) Potential
Disulfide bond275 ↔ 367 By similarity
Disulfide bond345 ↔ 359 By similarity

Experimental info

Sequence conflict2621E → V in CAA53510. Ref.1
Sequence conflict2881A → G in CAA53510. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35246 [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 4A74B7593508AE5D

FASTA36937,405
        10         20         30         40         50         60 
MLLLPLSVLL LLTQPWRSLG AEMKIYSQKT MANACTLVMC SPPEDGLPGR DGRDGREGPR 

        70         80         90        100        110        120 
GEKGDPGSPG PAGRAGMPGP AGPIGLKGDN GSAGEPGPKG DTGPPGPPGM PGPAGREGPS 

       130        140        150        160        170        180 
GKQGSMGPPG TPGPKGDTGP KGGVGAPGIQ GSPGPAGLKG ERGAPGEPGA PGRAGAPGPA 

       190        200        210        220        230        240 
GAIGPQGPSG ARGPPGLKGD RGTPGERGAK GESGLAEVNA LRQRVGILEG QLQRLQNAFS 

       250        260        270        280        290        300 
QYKKAMLFPN GRSVGEKIFK TEGSEKTFQD AQQICTQAGG QLPSPRSAAE NEALTQLATA 

       310        320        330        340        350        360 
QNKAAFLSMS DTRKEGTFIY PTGEPLVYSN WAPQEPNNDG GSENCVEIFP NGKWNDKVCG 


EQRLVICEF 

« Hide

References

[1]"Structural similarity between bovine conglutinin and bovine lung surfactant protein D and demonstration of liver as a site of synthesis of conglutinin."
Lim B.L., Lu J., Reid K.B.M.
Immunology 78:159-165(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 208-247.
Tissue: Lung.
[2]"Bovine surfactant protein D: genomic characterization, chromosomal localization and expression analysis."
Gjerstorff M., Hansen S., Madsen J., Bendixen C., Holmskov U.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75911 mRNA. Translation: CAA53510.1.
AJ548848, AJ548849, AJ548850 Genomic DNA. Translation: CAD69922.1.
PIRS33603.
RefSeqNP_851369.1. NM_181026.2.
UniGeneBt.33887.

3D structure databases

ProteinModelPortalP35246.
SMRP35246. Positions 216-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid159354. 2 interactions.
STRING9913.ENSBTAP00000018649.

Proteomic databases

PRIDEP35246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000008579; ENSBTAP00000008579; ENSBTAG00000046421.
GeneID282072.
KEGGbta:282072.

Organism-specific databases

CTD6441.

Phylogenomic databases

GeneTreeENSGT00700000104102.
HOGENOMHOG000085660.
HOVERGENHBG108270.
KOK10068.
OMAEMKTYSQ.
OrthoDBEOG7HXCVB.
TreeFamTF330481.

Family and domain databases

Gene3D1.20.5.360. 1 hit.
3.10.100.10. 1 hit.
InterProIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
IPR015097. Surfac_D-trimer.
[Graphical view]
PfamPF01391. Collagen. 2 hits.
PF00059. Lectin_C. 1 hit.
PF09006. Surfac_D-trimer. 1 hit.
[Graphical view]
SMARTSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMSSF56436. SSF56436. 1 hit.
PROSITEPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805922.

Entry information

Entry nameSFTPD_BOVIN
AccessionPrimary (citable) accession number: P35246
Secondary accession number(s): Q863A1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 27, 2006
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families