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Protein

Pulmonary surfactant-associated protein D

Gene

SFTPD

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  1. innate immune response Source: UniProtKB-KW
  2. lung alveolus development Source: UniProtKB
  3. respiratory gaseous exchange Source: UniProtKB-KW
  4. surfactant homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Gaseous exchange, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Lectin

Enzyme and pathway databases

ReactomeiREACT_211704. Toll Like Receptor 4 (TLR4) Cascade.
REACT_215402. Signal regulatory protein (SIRP) family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein D
Short name:
PSP-D
Short name:
SP-D
Alternative name(s):
Lung surfactant protein D
Gene namesi
Name:SFTPD
Synonyms:SFTP4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 28

Subcellular locationi

GO - Cellular componenti

  1. collagen trimer Source: UniProtKB-KW
  2. extracellular space Source: UniProtKB-SubCell
  3. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 369349Pulmonary surfactant-associated protein DPRO_0000017464Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351S-nitrosocysteineBy similarity
Modified residuei40 – 401S-nitrosocysteineBy similarity
Modified residuei78 – 7814-hydroxyprolineBy similarity
Modified residuei87 – 8715-hydroxylysineBy similarity
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Modified residuei96 – 9614-hydroxyprolineBy similarity
Modified residuei99 – 9915-hydroxylysineBy similarity
Modified residuei165 – 16514-hydroxyprolineBy similarity
Modified residuei171 – 17114-hydroxyprolineBy similarity
Disulfide bondi275 ↔ 367PROSITE-ProRule annotation
Disulfide bondi345 ↔ 359PROSITE-ProRule annotation

Post-translational modificationi

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity
S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, S-nitrosylation

Proteomic databases

PRIDEiP35246.

Interactioni

Subunit structurei

Oligomeric complex of 4 set of homotrimers.

Protein-protein interaction databases

BioGridi159354. 2 interactions.
STRINGi9913.ENSBTAP00000018649.

Structurei

3D structure databases

ProteinModelPortaliP35246.
SMRiP35246. Positions 216-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 216171Collagen-likeAdd
BLAST
Domaini254 – 369116C-type lectinPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili217 – 24832Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the SFTPD family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Coiled coil, Collagen, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP35246.
KOiK10068.
OMAiEMKTYSQ.
OrthoDBiEOG7HXCVB.
TreeFamiTF330481.

Family and domain databases

Gene3Di1.20.5.360. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
IPR015097. Surfac_D-trimer.
[Graphical view]
PfamiPF01391. Collagen. 2 hits.
PF00059. Lectin_C. 1 hit.
PF09006. Surfac_D-trimer. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35246-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLPLSVLL LLTQPWRSLG AEMKIYSQKT MANACTLVMC SPPEDGLPGR
60 70 80 90 100
DGRDGREGPR GEKGDPGSPG PAGRAGMPGP AGPIGLKGDN GSAGEPGPKG
110 120 130 140 150
DTGPPGPPGM PGPAGREGPS GKQGSMGPPG TPGPKGDTGP KGGVGAPGIQ
160 170 180 190 200
GSPGPAGLKG ERGAPGEPGA PGRAGAPGPA GAIGPQGPSG ARGPPGLKGD
210 220 230 240 250
RGTPGERGAK GESGLAEVNA LRQRVGILEG QLQRLQNAFS QYKKAMLFPN
260 270 280 290 300
GRSVGEKIFK TEGSEKTFQD AQQICTQAGG QLPSPRSAAE NEALTQLATA
310 320 330 340 350
QNKAAFLSMS DTRKEGTFIY PTGEPLVYSN WAPQEPNNDG GSENCVEIFP
360
NGKWNDKVCG EQRLVICEF
Length:369
Mass (Da):37,405
Last modified:June 27, 2006 - v2
Checksum:i4A74B7593508AE5D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2621E → V in CAA53510 (PubMed:8436402).Curated
Sequence conflicti288 – 2881A → G in CAA53510 (PubMed:8436402).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75911 mRNA. Translation: CAA53510.1.
AJ548848, AJ548849, AJ548850 Genomic DNA. Translation: CAD69922.1.
PIRiS33603.
RefSeqiNP_851369.1. NM_181026.2.
UniGeneiBt.33887.

Genome annotation databases

EnsembliENSBTAT00000008579; ENSBTAP00000008579; ENSBTAG00000046421.
GeneIDi282072.
KEGGibta:282072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75911 mRNA. Translation: CAA53510.1.
AJ548848, AJ548849, AJ548850 Genomic DNA. Translation: CAD69922.1.
PIRiS33603.
RefSeqiNP_851369.1. NM_181026.2.
UniGeneiBt.33887.

3D structure databases

ProteinModelPortaliP35246.
SMRiP35246. Positions 216-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159354. 2 interactions.
STRINGi9913.ENSBTAP00000018649.

Proteomic databases

PRIDEiP35246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008579; ENSBTAP00000008579; ENSBTAG00000046421.
GeneIDi282072.
KEGGibta:282072.

Organism-specific databases

CTDi6441.

Phylogenomic databases

GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiP35246.
KOiK10068.
OMAiEMKTYSQ.
OrthoDBiEOG7HXCVB.
TreeFamiTF330481.

Enzyme and pathway databases

ReactomeiREACT_211704. Toll Like Receptor 4 (TLR4) Cascade.
REACT_215402. Signal regulatory protein (SIRP) family interactions.

Miscellaneous databases

NextBioi20805922.

Family and domain databases

Gene3Di1.20.5.360. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR008160. Collagen.
IPR015097. Surfac_D-trimer.
[Graphical view]
PfamiPF01391. Collagen. 2 hits.
PF00059. Lectin_C. 1 hit.
PF09006. Surfac_D-trimer. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural similarity between bovine conglutinin and bovine lung surfactant protein D and demonstration of liver as a site of synthesis of conglutinin."
    Lim B.L., Lu J., Reid K.B.M.
    Immunology 78:159-165(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 208-247.
    Tissue: Lung.
  2. "Bovine surfactant protein D: genomic characterization, chromosomal localization and expression analysis."
    Gjerstorff M., Hansen S., Madsen J., Bendixen C., Holmskov U.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiSFTPD_BOVIN
AccessioniPrimary (citable) accession number: P35246
Secondary accession number(s): Q863A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: June 27, 2006
Last modified: March 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.