P35243 (RECO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Recoverin

Alternative name(s):
Cancer-associated retinopathy protein
Short name=Protein CAR

Gene names

Name:	RCVRN
Synonyms:	RCV1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
Tissue specificity	Retina and pineal gland.
Miscellaneous	Binds two calcium ions; one with high affinity, the other with low affinity.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	visual perception Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Traceable author statement. Source: ProtInc calcium sensitive guanylate cyclase activator activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 200

199

Recoverin

PRO_0000073759

Regions

Domain

25 – 60

36

EF-hand 1

Domain

61 – 96

36

EF-hand 2

Domain

97 – 132

36

EF-hand 3

Domain

147 – 182

36

EF-hand 4

Calcium binding

74 – 85

12

1; low affinity By similarity

Calcium binding

110 – 121

12

2; high affinity By similarity

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine By similarity

Secondary structure

1

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200

Helix Strand Turn

Details...

Helix

11 – 20

10

Helix

25 – 38

14

Helix

46 – 55

10

Helix

63 – 73

11

Helix

83 – 93

11

Helix

102 – 109

8

Helix

119 – 133

15

Helix

135 – 139

5

Turn

143 – 145

3

Helix

148 – 158

11

Helix

169 – 173

5

Helix

180 – 186

7

Sequences

	Sequence		Length	Mass (Da)
	P35243 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7B1FA91EF19E6122	FASTA	200	23,130
	10 20 30 40 50 60 MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT 70 80 90 100 110 120 DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN 130 140 150 160 170 180 EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK 190 200 EILRLIQFEP QKVKEKMKNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of human retinal genes: recoverin cDNA and gene." Murakami A., Yajima T., Inana G. Biochem. Biophys. Res. Commun. 187:234-244(1992) [PubMed: 1387789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Molecular cloning and nucleotide sequence of a cDNA encoding recoverin from human retina." Wiechmann A.F., Hammarback J.A. Exp. Eye Res. 56:463-470(1993) [PubMed: 8500558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	"The cancer-associated retinopathy antigen is a recoverin-like protein." Thirkill C.E., Tait R.C., Tyler N.K., Roth A.M., Keltner J.L. Invest. Ophthalmol. Vis. Sci. 33:2768-2772(1992) [PubMed: 1388144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Expression of a photoreceptor protein, recoverin, as a cancer-associated retinopathy autoantigen in human lung cancer cell lines." Matsubara S., Yamaji Y., Sato M., Fujita J., Takahara J. Br. J. Cancer 74:1419-1422(1996) [PubMed: 8912538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[6]	"A photoreceptor calcium binding protein is recognized by autoantibodies obtained from patients with cancer-associated retinopathy." Polans A.S., Buczylko J., Crabb J., Palczewski K. J. Cell Biol. 112:981-989(1991) [PubMed: 1999465] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[7]	"Crystal structure of human recoverin at 2.2 A resolution." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

S43855 mRNA. Translation: AAB23163.1.
S45545 mRNA. Translation: AAB23392.1.
S62028 mRNA. Translation: AAB26894.1.
AB001838 mRNA. Translation: BAA19460.1.
BC001720 mRNA. Translation: AAH01720.1.

IPI

IPI00219720.

PIR

JC1227.

RefSeq

NP_002894.1. NM_002903.2.

UniGene

Hs.80539.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D8N	X-ray	2.20	A	1-200	[»]

ProteinModelPortal

P35243.

SMR

P35243. Positions 1-197.

ModBase

Search...

Protein-protein interaction databases

IntAct

P35243. 5 interactions.

MINT

MINT-2862634.

STRING

P35243.

PTM databases

PhosphoSite

P35243.

Polymorphism databases

DMDM

464600.

Proteomic databases

PRIDE

P35243.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000226193; ENSP00000226193; ENSG00000109047.

GeneID

5957.

KEGG

hsa:5957.

UCSC

uc002gme.1. human.

Organism-specific databases

CTD

5957.

GeneCards

GC17M009799.

H-InvDB

HIX0013532.

HGNC

HGNC:9937. RCVRN.

MIM

179618. gene.

neXtProt

NX_P35243.

PharmGKB

PA162400987.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG13132.

HOGENOM

HBG746798.

HOVERGEN

HBG108179.

InParanoid

P35243.

OMA

LKECPSG.

OrthoDB

EOG46MBKK.

PhylomeDB

P35243.

Gene expression databases

ArrayExpress

P35243.

Bgee

P35243.

CleanEx

HS_RCVRN.

Genevestigator

P35243.

GermOnline

ENSG00000109047. Homo sapiens.

Family and domain databases

InterPro

IPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
IPR001125. Recoverin.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

KO

K13764.

Pfam

PF00036. efhand. 1 hit.
[Graphical view]

PRINTS

PR00450. RECOVERIN.

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

23200.

SOURCE

Search...

Entry information

Entry name

RECO_HUMAN

Accession

Primary (citable) accession number: P35243

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.