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Protein

Tyrosine-protein phosphatase non-receptor type 5

Gene

Ptpn5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651SubstrateBy similarity
Active sitei300 – 3001Phosphocysteine intermediatePROSITE-ProRule annotation
Binding sitei344 – 3441SubstrateBy similarity

GO - Molecular functioni

  • glutamate receptor binding Source: RGD
  • mitogen-activated protein kinase binding Source: RGD
  • protein kinase binding Source: RGD
  • protein tyrosine phosphatase activity Source: RGD

GO - Biological processi

  • exploration behavior Source: RGD
  • peptidyl-tyrosine dephosphorylation Source: RGD
  • positive regulation of long-term synaptic potentiation Source: RGD
  • positive regulation of neuron death Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of protein import into nucleus, translocation Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of protein targeting to mitochondrion Source: RGD
  • positive regulation of receptor internalization Source: RGD
  • positive regulation of response to drug Source: RGD
  • response to amphetamine Source: RGD
  • response to drug Source: RGD
  • response to immobilization stress Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 5 (EC:3.1.3.48)
Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name:
STEP
Gene namesi
Name:Ptpn5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3448. Ptpn5.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • cytosol Source: RGD
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • perikaryon Source: RGD
  • proximal dendrite Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Tyrosine-protein phosphatase non-receptor type 5PRO_0000094757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491Phosphoserine; by PKABy similarity
Modified residuei59 – 591Phosphothreonine; by MAPKBy similarity
Modified residuei72 – 721Phosphoserine; by MAPKBy similarity

Post-translational modificationi

Phosphorylation at Ser-49 by PKA deactivates PTPN5. Phosphorylation at Thr-59 and Ser-72 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35234.
PRIDEiP35234.

PTM databases

PhosphoSiteiP35234.

Expressioni

Tissue specificityi

Expressed in the central nervous system except in the cerebellum. Enriched within the striatum relative to other brain areas.

Gene expression databases

GenevisibleiP35234. RN.

Interactioni

Protein-protein interaction databases

BioGridi248266. 1 interaction.
IntActiP35234. 1 interaction.
MINTiMINT-713354.
STRINGi10116.ENSRNOP00000018860.

Structurei

3D structure databases

ProteinModelPortaliP35234.
SMRiP35234. Positions 81-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 359256Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 3067Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiP35234.
KOiK18018.
PhylomeDBiP35234.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKVEDDFL DLDAVPETPV FDCVMDIKPE ADPTSLTVKS MGLQERRGSN
60 70 80 90 100
VSLTLDMCTP GCNEEGFGYL VSPREESAHE YLLSASRVLR AEELHEKALD
110 120 130 140 150
PFLLQAEFFE IPMNFVDPKE YDIPGLVRKN RYKTILPNPH SRVRLTSPDP
160 170 180 190 200
EDPLSSYINA NYIRGYNGEE KVYIATQGPI VSTVVDFWRM VWQERTPIIV
210 220 230 240 250
MITNIEEMNE KCTEYWPEEQ VVHDGVEITV QKVIHTEDYR LRLISLRRGT
260 270 280 290 300
EERGLKHYWF TSWPDQKTPD RAPPLLHLVR EVEEAAQQEG PHCSPIIVHC
310 320 330 340 350
SAGIGRTGCF IATSICCQQL RREGVVDILK TTCQLRQDRG GMIQTCEQYQ
360
FVHHAMSLYE KQLSLQSSE
Length:369
Mass (Da):42,366
Last modified:February 1, 1995 - v2
Checksum:i7CD8A77EE238C64B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S49400 mRNA. Translation: AAB19491.1.
PIRiA41147.
RefSeqiNP_062126.3. NM_019253.3.
UniGeneiRn.10618.

Genome annotation databases

GeneIDi29644.
KEGGirno:29644.
UCSCiRGD:3448. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S49400 mRNA. Translation: AAB19491.1.
PIRiA41147.
RefSeqiNP_062126.3. NM_019253.3.
UniGeneiRn.10618.

3D structure databases

ProteinModelPortaliP35234.
SMRiP35234. Positions 81-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248266. 1 interaction.
IntActiP35234. 1 interaction.
MINTiMINT-713354.
STRINGi10116.ENSRNOP00000018860.

Chemistry

ChEMBLiCHEMBL2429710.

PTM databases

PhosphoSiteiP35234.

Proteomic databases

PaxDbiP35234.
PRIDEiP35234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29644.
KEGGirno:29644.
UCSCiRGD:3448. rat.

Organism-specific databases

CTDi84867.
RGDi3448. Ptpn5.

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000294188.
HOVERGENiHBG001594.
InParanoidiP35234.
KOiK18018.
PhylomeDBiP35234.

Miscellaneous databases

NextBioi609910.
PROiP35234.

Gene expression databases

GenevisibleiP35234. RN.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR008356. Tyr_Pase_KIM-con.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR01778. KIMPTPASE.
PR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum."
    Lombroso P.J., Murdoch G., Lerner M.
    Proc. Natl. Acad. Sci. U.S.A. 88:7242-7246(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPTN5_RAT
AccessioniPrimary (citable) accession number: P35234
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.