P35234 (PTN5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 5
EC=3.1.3.48

Alternative name(s):
Neural-specific protein-tyrosine phosphatase
Striatum-enriched protein-tyrosine phosphatase
Short name=STEP

Gene names

Name:

Ptpn5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in the central nervous system except in the cerebellum. Enriched within the striatum relative to other brain areas.
Post-translational modification	Phosphorylation at Ser-49 by PKA deactivates PTPN5. Phosphorylation at Thr-59 and Ser-72 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Tyrosine-protein phosphatase non-receptor type 5		PRO_0000094757
Regions
	Domain	104 – 359	256	Tyrosine-protein phosphatase
	Region	300 – 306	7	Substrate binding By similarity
Sites
	Active site	300	1	Phosphocysteine intermediate By similarity
	Binding site	265	1	Substrate By similarity
	Binding site	344	1	Substrate By similarity
Amino acid modifications
	Modified residue	49	1	Phosphoserine; by PKA By similarity
	Modified residue	59	1	Phosphothreonine; by MAPK By similarity
	Modified residue	72	1	Phosphoserine; by MAPK By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35234 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 7CD8A77EE238C64B	FASTA	369	42,366
	10 20 30 40 50 60 MEEKVEDDFL DLDAVPETPV FDCVMDIKPE ADPTSLTVKS MGLQERRGSN VSLTLDMCTP 70 80 90 100 110 120 GCNEEGFGYL VSPREESAHE YLLSASRVLR AEELHEKALD PFLLQAEFFE IPMNFVDPKE 130 140 150 160 170 180 YDIPGLVRKN RYKTILPNPH SRVRLTSPDP EDPLSSYINA NYIRGYNGEE KVYIATQGPI 190 200 210 220 230 240 VSTVVDFWRM VWQERTPIIV MITNIEEMNE KCTEYWPEEQ VVHDGVEITV QKVIHTEDYR 250 260 270 280 290 300 LRLISLRRGT EERGLKHYWF TSWPDQKTPD RAPPLLHLVR EVEEAAQQEG PHCSPIIVHC 310 320 330 340 350 360 SAGIGRTGCF IATSICCQQL RREGVVDILK TTCQLRQDRG GMIQTCEQYQ FVHHAMSLYE KQLSLQSSE

References

[1]	"Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum." Lombroso P.J., Murdoch G., Lerner M. Proc. Natl. Acad. Sci. U.S.A. 88:7242-7246(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S49400 mRNA. Translation: AAB19491.1.
IPI	IPI00196688.
PIR	A41147.
RefSeq	NP_062126.3. NM_019253.3.
UniGene	Rn.10618.
3D structure databases
ProteinModelPortal	P35234.
SMR	P35234. Positions 81-364.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-713354.
STRING	10116.ENSRNOP00000018860.
PTM databases
PhosphoSite	P35234.
Proteomic databases
PaxDb	P35234.
PRIDE	P35234.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29644.
KEGG	rno:29644.
UCSC	RGD:3448. rat.
Organism-specific databases
CTD	84867.
RGD	3448. Ptpn5.
Phylogenomic databases
eggNOG	COG5599.
HOGENOM	HOG000294188.
HOVERGEN	HBG001594.
InParanoid	P35234.
KO	K04458.
OrthoDB	EOG4229JX.
Gene expression databases
ArrayExpress	P35234.
Genevestigator	P35234.
GermOnline	ENSRNOG00000013981. Rattus norvegicus.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR008356. Tyr_Pase_KIM-con. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR01778. KIMPTPASE. PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	609910.

Entry information

Entry name

PTN5_RAT

Accession

Primary (citable) accession number: P35234

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program