P35233 (PTN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein phosphatase non-receptor type 2
EC=3.1.3.48

Alternative name(s):
Protein-tyrosine phosphatase PTP-S

Gene names

Name:

Ptpn2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds single-stranded and double-stranded DNA with high nonspecific affinity through its C-terminal region.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit structure	Interacts with RMDN3. Interacts with TMED9 (By. similarity).
Subcellular location	Cytoplasm Ref.5. Isoform 1: Endoplasmic reticulum. Nucleus membrane Ref.5. Isoform 2: Nucleus. Note: Predominantly localizes to chromatin. Ref.5
Tissue specificity	Does not show tissue- or cell-type specificity although levels of transcription show variability. Macrophages showed higher levels of expression than lymphocytes.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	insulin receptor signaling pathway Inferred from electronic annotation. Source: Compara
Cellular_component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Traceable author statement Ref.1. Source: RGD non-membrane spanning protein tyrosine phosphatase activity Inferred from direct assay Ref.4. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P35233-1)

Also known as: PTP-S4;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P35233-2)

Also known as: PTP-S2;

The sequence of this isoform differs from the canonical sequence as follows:
     377-410: Missing.

Isoform 3 (identifier: P35233-3)

Also known as: PTP-S3;

The sequence of this isoform differs from the canonical sequence as follows:
     287-305: Missing.

Note: Minor.

Isoform 4 (identifier: P35233-4)

Also known as: PTP-S1;

The sequence of this isoform differs from the canonical sequence as follows:
     287-305: Missing.
     377-410: Missing.

Note: Minor.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Tyrosine-protein phosphatase non-receptor type 2		PRO_0000094754
Regions
	Domain	5 – 275	271	Tyrosine-protein phosphatase
	Region	216 – 222	7	Substrate binding By similarity
Sites
	Active site	216	1	Phosphocysteine intermediate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	260	1	Substrate By similarity
Amino acid modifications
	Modified residue	298	1	Phosphoserine By similarity
	Modified residue	304	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	287 – 305	19	Missing in isoform 3 and isoform 4.		VSP_042001
	Alternative sequence	377 – 410	34	Missing in isoform 2 and isoform 4.		VSP_042002

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (PTP-S4) [UniParc]. Last modified November 16, 2011. Version 2. Checksum: E8FB1E670FFC5308	FASTA	416	48,446
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTRAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQILLS MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQKRWK ELSKEDLSPV 310 320 330 340 350 360 CRHSQNRTMT EKYNGKRIGS EDEKLTGLSS KVPDTVEESS ESILRKRIRE DRKATTAQKV 370 380 390 400 410 QQMRQRLNET ERKRKRWLYW QPILTKMGFV SVILVGALVG WTLLFQLNVL PRLTDT
	Isoform 2 (PTP-S2) [UniParc]. Checksum: 0455FE00D3162D8F	FASTA	382	44,544
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTRAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQILLS MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQKRWK ELSKEDLSPV 310 320 330 340 350 360 CRHSQNRTMT EKYNGKRIGS EDEKLTGLSS KVPDTVEESS ESILRKRIRE DRKATTAQKV 370 380 QQMRQRLNET ERKRKRPRLT DT
	Isoform 3 (PTP-S3) [UniParc]. Checksum: 5B7F4DD9463D779F	FASTA	397	46,138
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTRAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQILLS MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQNRTM TEKYNGKRIG 310 320 330 340 350 360 SEDEKLTGLS SKVPDTVEES SESILRKRIR EDRKATTAQK VQQMRQRLNE TERKRKRWLY 370 380 390 WQPILTKMGF VSVILVGALV GWTLLFQLNV LPRLTDT
	Isoform 4 (PTP-S1) [UniParc]. Checksum: B417C04466D24715	FASTA	363	42,236
	10 20 30 40 50 60 MSATIEREFE ELDAQCRWQP LYLEIRNESH DYPHRVAKFP ENRNRNRYRD VSPYDHSRVK 70 80 90 100 110 120 LQSAENDYIN ASLVDIEEAQ RSYILTQGPL PNTCCHFWLM VWQQKTRAVV MLNRTVEKES 130 140 150 160 170 180 VKCAQYWPTD DREMVFKETG FSVKLLSEDV KSYYTVHLLQ LENINSGETR TISHFHYTTW 190 200 210 220 230 240 PDFGVPESPA SFLNFLFKVR ESGSLNPDHG PAVIHCSAGI GRSGTFSLVD TCLVLMEKGE 250 260 270 280 290 300 DVNVKQILLS MRKYRMGLIQ TPDQLRFSYM AIIEGAKYTK GDSNIQNRTM TEKYNGKRIG 310 320 330 340 350 360 SEDEKLTGLS SKVPDTVEES SESILRKRIR EDRKATTAQK VQQMRQRLNE TERKRKRPRL TDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of a protein-tyrosine phosphatase showing homology with transcription factors Fos and Jun." Swarup G., Kamatkar S., Radha V., Rema V. FEBS Lett. 280:65-69(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis.
[3]	"Binding of a protein-tyrosine phosphatase to DNA through its carboxy-terminal noncatalytic domain." Radha V., Kamatkar S., Swarup G. Biochemistry 32:2194-2201(1993) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING.
[4]	"Alternative splicing generates four different forms of a non-transmembrane protein tyrosine phosphatase mRNA." Reddy R.S., Swarup G. DNA Cell Biol. 14:1007-1015(1995) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[5]	"Two splice variants of a tyrosine phosphatase differ in substrate specificity, DNA binding, and subcellular location." Kamatkar S., Radha V., Nambirajan S., Reddy R.S., Swarup G. J. Biol. Chem. 271:26755-26761(1996) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION (ISOFORMS 1 AND 2).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58828 mRNA. Translation: CAA41633.1. BC078758 mRNA. Translation: AAH78758.1.
IPI	IPI00196687.
PIR	S14294.
RefSeq	NP_446442.1. NM_053990.1.
UniGene	Rn.33497.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P35233. 1 interaction.
STRING	10116.ENSRNOP00000023763.
Proteomic databases
PaxDb	P35233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	117063.
KEGG	rno:117063.
UCSC	RGD:620710. rat.
Organism-specific databases
CTD	5771.
RGD	620710. Ptpn2.
Phylogenomic databases
eggNOG	COG5599.
HOGENOM	HOG000273908.
HOVERGEN	HBG008321.
InParanoid	P35233.
KO	K01104.
OrthoDB	EOG461447.
Gene expression databases
ArrayExpress	P35233.
Genevestigator	P35233.
GermOnline	ENSRNOG00000017453. Rattus norvegicus.
Family and domain databases
InterPro	IPR012265. Ptpn1/Ptpn2. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
PANTHER	PTHR19134:SF57. PTHR19134:SF57. 1 hit.
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PIRSF	PIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619930.

Entry information

Entry name

PTN2_RAT

Accession

Primary (citable) accession number: P35233

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	November 16, 2011
Last modified:	May 29, 2013
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program