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P35227

- PCGF2_HUMAN

UniProt

P35227 - PCGF2_HUMAN

Protein

Polycomb group RING finger protein 2

Gene

PCGF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. DNA binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. sequence-specific DNA binding transcription factor activity Source: ProtInc
    5. ubiquitin-protein transferase activity Source: Ensembl
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. anterior/posterior pattern specification Source: Ensembl
    2. embryonic skeletal system morphogenesis Source: Ensembl
    3. histone acetylation Source: Ensembl
    4. in utero embryonic development Source: Ensembl
    5. negative regulation of apoptotic signaling pathway Source: Ensembl
    6. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polycomb group RING finger protein 2
    Alternative name(s):
    DNA-binding protein Mel-18
    RING finger protein 110
    Zinc finger protein 144
    Gene namesi
    Name:PCGF2
    Synonyms:MEL18, RNF110, ZNF144
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:12929. PCGF2.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nuclear body Source: Ensembl
    2. nuclear chromatin Source: UniProtKB
    3. nucleus Source: UniProtKB
    4. PcG protein complex Source: UniProtKB
    5. PRC1 complex Source: UniProtKB
    6. sex chromatin Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA37516.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Polycomb group RING finger protein 2PRO_0000055984Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei344 – 3441Phosphothreonine1 Publication

    Post-translational modificationi

    Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP35227.
    PaxDbiP35227.
    PRIDEiP35227.

    PTM databases

    PhosphoSiteiP35227.

    Expressioni

    Tissue specificityi

    Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.

    Gene expression databases

    ArrayExpressiP35227.
    BgeeiP35227.
    CleanExiHS_PCGF2.
    GenevestigatoriP35227.

    Organism-specific databases

    HPAiHPA047732.

    Interactioni

    Subunit structurei

    Exists as both a monomer and homodimer By similarity. Component of a PRC1-like complex. Interacts WITH CBX8, RING1 AND RNF2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CBX7O959313EBI-2129767,EBI-3923843
    CBX8Q9HC527EBI-2129767,EBI-712912
    RING1Q065875EBI-2129767,EBI-752313
    RNF2Q994967EBI-2129767,EBI-722416
    UBCP0CG482EBI-2129767,EBI-3390054
    USP11P517845EBI-2129767,EBI-306876
    USP7Q930095EBI-2129767,EBI-302474

    Protein-protein interaction databases

    BioGridi113497. 45 interactions.
    DIPiDIP-41880N.
    IntActiP35227. 27 interactions.
    MINTiMINT-158325.
    STRINGi9606.ENSP00000354033.

    Structurei

    3D structure databases

    ProteinModelPortaliP35227.
    SMRiP35227. Positions 5-101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi81 – 9515Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi242 – 344103Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG304672.
    HOGENOMiHOG000231945.
    HOVERGENiHBG052826.
    InParanoidiP35227.
    KOiK11460.
    OMAiWAVESSC.
    OrthoDBiEOG77T14W.
    PhylomeDBiP35227.
    TreeFamiTF324206.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35227-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN    50
    KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA 100
    YPLTEVPNGS NEDRGEVLEQ EKGALSDDEI VSLSIEFYEG ARDRDEKKGP 150
    LENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEVLYEDE 200
    PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLAT VPTPSEGTNT 250
    SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP 300
    TPPSTASGAT TAANGGSLNC LQTPSSTSRG RKMTVNGAPV PPLT 344
    Length:344
    Mass (Da):37,788
    Last modified:February 1, 1994 - v1
    Checksum:iA910BCD4C0CE9927
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13969 mRNA. Translation: BAA03074.1.
    BX647429 mRNA. No translation available.
    AC006449 Genomic DNA. No translation available.
    CH471152 Genomic DNA. Translation: EAW60528.1.
    BC004858 mRNA. Translation: AAH04858.1.
    BC024255 mRNA. Translation: AAH24255.1.
    CCDSiCCDS32638.1.
    PIRiJN0717.
    RefSeqiNP_009075.1. NM_007144.2.
    XP_005257697.1. XM_005257640.1.
    XP_005257698.1. XM_005257641.2.
    XP_005257699.1. XM_005257642.1.
    XP_006722134.1. XM_006722071.1.
    XP_006725405.1. XM_006725342.1.
    XP_006725406.1. XM_006725343.1.
    XP_006725407.1. XM_006725344.1.
    XP_006725408.1. XM_006725345.1.
    UniGeneiHs.371617.
    Hs.713595.

    Genome annotation databases

    GeneIDi7703.
    KEGGihsa:7703.
    UCSCiuc002hqp.1. human.

    Polymorphism databases

    DMDMi462585.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13969 mRNA. Translation: BAA03074.1 .
    BX647429 mRNA. No translation available.
    AC006449 Genomic DNA. No translation available.
    CH471152 Genomic DNA. Translation: EAW60528.1 .
    BC004858 mRNA. Translation: AAH04858.1 .
    BC024255 mRNA. Translation: AAH24255.1 .
    CCDSi CCDS32638.1.
    PIRi JN0717.
    RefSeqi NP_009075.1. NM_007144.2.
    XP_005257697.1. XM_005257640.1.
    XP_005257698.1. XM_005257641.2.
    XP_005257699.1. XM_005257642.1.
    XP_006722134.1. XM_006722071.1.
    XP_006725405.1. XM_006725342.1.
    XP_006725406.1. XM_006725343.1.
    XP_006725407.1. XM_006725344.1.
    XP_006725408.1. XM_006725345.1.
    UniGenei Hs.371617.
    Hs.713595.

    3D structure databases

    ProteinModelPortali P35227.
    SMRi P35227. Positions 5-101.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 113497. 45 interactions.
    DIPi DIP-41880N.
    IntActi P35227. 27 interactions.
    MINTi MINT-158325.
    STRINGi 9606.ENSP00000354033.

    PTM databases

    PhosphoSitei P35227.

    Polymorphism databases

    DMDMi 462585.

    Proteomic databases

    MaxQBi P35227.
    PaxDbi P35227.
    PRIDEi P35227.

    Protocols and materials databases

    DNASUi 7703.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7703.
    KEGGi hsa:7703.
    UCSCi uc002hqp.1. human.

    Organism-specific databases

    CTDi 7703.
    GeneCardsi GC17M036890.
    HGNCi HGNC:12929. PCGF2.
    HPAi HPA047732.
    MIMi 600346. gene.
    neXtProti NX_P35227.
    PharmGKBi PA37516.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG304672.
    HOGENOMi HOG000231945.
    HOVERGENi HBG052826.
    InParanoidi P35227.
    KOi K11460.
    OMAi WAVESSC.
    OrthoDBi EOG77T14W.
    PhylomeDBi P35227.
    TreeFami TF324206.

    Miscellaneous databases

    GeneWikii PCGF2.
    GenomeRNAii 7703.
    NextBioi 29858.
    PROi P35227.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35227.
    Bgeei P35227.
    CleanExi HS_PCGF2.
    Genevestigatori P35227.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif."
      Ishida A., Asano H., Hasegawa M., Koseki H., Ono T., Yoshida M.C., Taniguchi M., Kanno M.
      Gene 129:249-255(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon endothelium.
    3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta and Uterus.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-344, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus."
      Maertens G.N., El Messaoudi-Aubert S., Racek T., Stock J.K., Nicholls J., Rodriguez-Niedenfuhr M., Gil J., Peters G.
      PLoS ONE 4:E6380-E6380(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, INTERACTION WITH CBX8; RING1 AND RNF2.
    8. "Interaction proteomics analysis of polycomb proteins defines distinct PRC1 Complexes in mammalian cells."
      Vandamme J., Volkel P., Rosnoblet C., Le Faou P., Angrand P.O.
      Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPCGF2_HUMAN
    AccessioniPrimary (citable) accession number: P35227
    Secondary accession number(s): A6NGD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3