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P35227

- PCGF2_HUMAN

UniProt

P35227 - PCGF2_HUMAN

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Protein

Polycomb group RING finger protein 2

Gene
PCGF2, MEL18, RNF110, ZNF144
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with BMI1; unlike BMI1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typeAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: Ensembl
  2. DNA binding Source: UniProtKB-KW
  3. protein binding Source: IntAct
  4. sequence-specific DNA binding transcription factor activity Source: ProtInc
  5. ubiquitin-protein transferase activity Source: Ensembl
  6. zinc ion binding Source: InterPro

GO - Biological processi

  1. anterior/posterior pattern specification Source: Ensembl
  2. embryonic skeletal system morphogenesis Source: Ensembl
  3. histone acetylation Source: Ensembl
  4. in utero embryonic development Source: Ensembl
  5. negative regulation of apoptotic signaling pathway Source: Ensembl
  6. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group RING finger protein 2
Alternative name(s):
DNA-binding protein Mel-18
RING finger protein 110
Zinc finger protein 144
Gene namesi
Name:PCGF2
Synonyms:MEL18, RNF110, ZNF144
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:12929. PCGF2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nuclear body Source: Ensembl
  2. nuclear chromatin Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. PcG protein complex Source: UniProtKB
  5. PRC1 complex Source: UniProtKB
  6. sex chromatin Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37516.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Polycomb group RING finger protein 2PRO_0000055984Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei344 – 3441Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35227.
PaxDbiP35227.
PRIDEiP35227.

PTM databases

PhosphoSiteiP35227.

Expressioni

Tissue specificityi

Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.

Gene expression databases

ArrayExpressiP35227.
BgeeiP35227.
CleanExiHS_PCGF2.
GenevestigatoriP35227.

Organism-specific databases

HPAiHPA047732.

Interactioni

Subunit structurei

Exists as both a monomer and homodimer By similarity. Component of a PRC1-like complex. Interacts WITH CBX8, RING1 AND RNF2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CBX7O959313EBI-2129767,EBI-3923843
CBX8Q9HC527EBI-2129767,EBI-712912
RING1Q065875EBI-2129767,EBI-752313
RNF2Q994967EBI-2129767,EBI-722416
UBCP0CG482EBI-2129767,EBI-3390054
USP11P517845EBI-2129767,EBI-306876
USP7Q930095EBI-2129767,EBI-302474

Protein-protein interaction databases

BioGridi113497. 45 interactions.
DIPiDIP-41880N.
IntActiP35227. 27 interactions.
MINTiMINT-158325.
STRINGi9606.ENSP00000354033.

Structurei

3D structure databases

ProteinModelPortaliP35227.
SMRiP35227. Positions 5-101.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9515Nuclear localization signal Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi242 – 344103Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG304672.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP35227.
KOiK11460.
OMAiWAVESSC.
OrthoDBiEOG77T14W.
PhylomeDBiP35227.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35227-1 [UniParc]FASTAAdd to Basket

« Hide

MHRTTRIKIT ELNPHLMCAL CGGYFIDATT IVECLHSFCK TCIVRYLETN    50
KYCPMCDVQV HKTRPLLSIR SDKTLQDIVY KLVPGLFKDE MKRRRDFYAA 100
YPLTEVPNGS NEDRGEVLEQ EKGALSDDEI VSLSIEFYEG ARDRDEKKGP 150
LENGDGDKEK TGVRFLRCPA AMTVMHLAKF LRNKMDVPSK YKVEVLYEDE 200
PLKEYYTLMD IAYIYPWRRN GPLPLKYRVQ PACKRLTLAT VPTPSEGTNT 250
SGASECESVS DKAPSPATLP ATSSSLPSPA TPSHGSPSSH GPPATHPTSP 300
TPPSTASGAT TAANGGSLNC LQTPSSTSRG RKMTVNGAPV PPLT 344
Length:344
Mass (Da):37,788
Last modified:February 1, 1994 - v1
Checksum:iA910BCD4C0CE9927
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13969 mRNA. Translation: BAA03074.1.
BX647429 mRNA. No translation available.
AC006449 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60528.1.
BC004858 mRNA. Translation: AAH04858.1.
BC024255 mRNA. Translation: AAH24255.1.
CCDSiCCDS32638.1.
PIRiJN0717.
RefSeqiNP_009075.1. NM_007144.2.
XP_005257697.1. XM_005257640.1.
XP_005257698.1. XM_005257641.2.
XP_005257699.1. XM_005257642.1.
XP_006722134.1. XM_006722071.1.
XP_006725405.1. XM_006725342.1.
XP_006725406.1. XM_006725343.1.
XP_006725407.1. XM_006725344.1.
XP_006725408.1. XM_006725345.1.
UniGeneiHs.371617.
Hs.713595.

Genome annotation databases

EnsembliENST00000360797; ENSP00000354033; ENSG00000056661.
ENST00000580830; ENSP00000461961; ENSG00000056661.
ENST00000581345; ENSP00000463440; ENSG00000056661.
GeneIDi7703.
KEGGihsa:7703.
UCSCiuc002hqp.1. human.

Polymorphism databases

DMDMi462585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D13969 mRNA. Translation: BAA03074.1 .
BX647429 mRNA. No translation available.
AC006449 Genomic DNA. No translation available.
CH471152 Genomic DNA. Translation: EAW60528.1 .
BC004858 mRNA. Translation: AAH04858.1 .
BC024255 mRNA. Translation: AAH24255.1 .
CCDSi CCDS32638.1.
PIRi JN0717.
RefSeqi NP_009075.1. NM_007144.2.
XP_005257697.1. XM_005257640.1.
XP_005257698.1. XM_005257641.2.
XP_005257699.1. XM_005257642.1.
XP_006722134.1. XM_006722071.1.
XP_006725405.1. XM_006725342.1.
XP_006725406.1. XM_006725343.1.
XP_006725407.1. XM_006725344.1.
XP_006725408.1. XM_006725345.1.
UniGenei Hs.371617.
Hs.713595.

3D structure databases

ProteinModelPortali P35227.
SMRi P35227. Positions 5-101.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 113497. 45 interactions.
DIPi DIP-41880N.
IntActi P35227. 27 interactions.
MINTi MINT-158325.
STRINGi 9606.ENSP00000354033.

PTM databases

PhosphoSitei P35227.

Polymorphism databases

DMDMi 462585.

Proteomic databases

MaxQBi P35227.
PaxDbi P35227.
PRIDEi P35227.

Protocols and materials databases

DNASUi 7703.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000360797 ; ENSP00000354033 ; ENSG00000056661 .
ENST00000580830 ; ENSP00000461961 ; ENSG00000056661 .
ENST00000581345 ; ENSP00000463440 ; ENSG00000056661 .
GeneIDi 7703.
KEGGi hsa:7703.
UCSCi uc002hqp.1. human.

Organism-specific databases

CTDi 7703.
GeneCardsi GC17M036890.
HGNCi HGNC:12929. PCGF2.
HPAi HPA047732.
MIMi 600346. gene.
neXtProti NX_P35227.
PharmGKBi PA37516.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG304672.
HOGENOMi HOG000231945.
HOVERGENi HBG052826.
InParanoidi P35227.
KOi K11460.
OMAi WAVESSC.
OrthoDBi EOG77T14W.
PhylomeDBi P35227.
TreeFami TF324206.

Miscellaneous databases

GeneWikii PCGF2.
GenomeRNAii 7703.
NextBioi 29858.
PROi P35227.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35227.
Bgeei P35227.
CleanExi HS_PCGF2.
Genevestigatori P35227.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF00097. zf-C3HC4. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif."
    Ishida A., Asano H., Hasegawa M., Koseki H., Ono T., Yoshida M.C., Taniguchi M., Kanno M.
    Gene 129:249-255(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon endothelium.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta and Uterus.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-344, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. "Several distinct polycomb complexes regulate and co-localize on the INK4a tumor suppressor locus."
    Maertens G.N., El Messaoudi-Aubert S., Racek T., Stock J.K., Nicholls J., Rodriguez-Niedenfuhr M., Gil J., Peters G.
    PLoS ONE 4:E6380-E6380(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, INTERACTION WITH CBX8; RING1 AND RNF2.
  8. "Interaction proteomics analysis of polycomb proteins defines distinct PRC1 Complexes in mammalian cells."
    Vandamme J., Volkel P., Rosnoblet C., Le Faou P., Angrand P.O.
    Mol. Cell. Proteomics 0:0-0(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A PRC1-LIKE COMPLEX, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPCGF2_HUMAN
AccessioniPrimary (citable) accession number: P35227
Secondary accession number(s): A6NGD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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