Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polycomb complex protein BMI-1

Gene

BMI1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • promoter-specific chromatin binding Source: UniProtKB
  • RING-like zinc finger domain binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • covalent chromatin modification Source: UniProtKB-KW
  • hemopoiesis Source: UniProtKB
  • negative regulation of gene expression, epigenetic Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • positive regulation of fibroblast proliferation Source: BHF-UCL
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
  • protein sumoylation Source: Reactome
  • regulation of gene expression Source: BHF-UCL
  • segment specification Source: ProtInc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-2559580. Oxidative Stress Induced Senescence.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-4570464. SUMOylation of RNA binding proteins.
SIGNORiP35226.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb complex protein BMI-1
Alternative name(s):
Polycomb group RING finger protein 4
RING finger protein 51
Gene namesi
Name:BMI1
Synonyms:PCGF4, RNF51
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:1066. BMI1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • PcG protein complex Source: UniProtKB
  • PRC1 complex Source: UniProtKB
  • ubiquitin ligase complex Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

DisGeNETi648.
OpenTargetsiENSG00000168283.
PharmGKBiPA25376.

Polymorphism and mutation databases

BioMutaiBMI1.
DMDMi22258801.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559871 – 326Polycomb complex protein BMI-1Add BLAST326

Post-translational modificationi

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.By similarity1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiP35226.
MaxQBiP35226.
PaxDbiP35226.
PeptideAtlasiP35226.
PRIDEiP35226.

PTM databases

iPTMnetiP35226.
PhosphoSitePlusiP35226.

Expressioni

Gene expression databases

BgeeiENSG00000168283.
CleanExiHS_BMI1.
ExpressionAtlasiP35226. baseline and differential.
GenevisibleiP35226. HS.

Organism-specific databases

HPAiCAB011120.
HPA030471.
HPA030472.

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with RING1 and RING2 (By similarity). Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.By similarity9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • RING-like zinc finger domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107116. 370 interactors.
1529410. 5 interactors.
DIPiDIP-41879N.
IntActiP35226. 46 interactors.
MINTiMINT-158260.
STRINGi9606.ENSP00000365851.

Structurei

Secondary structure

1326
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Helixi9 – 12Combined sources4
Helixi13 – 15Combined sources3
Turni19 – 21Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi29 – 31Combined sources3
Turni32 – 34Combined sources3
Beta strandi36 – 39Combined sources4
Helixi40 – 46Combined sources7
Turni47 – 49Combined sources3
Turni54 – 56Combined sources3
Helixi65 – 68Combined sources4
Beta strandi69 – 71Combined sources3
Helixi73 – 82Combined sources10
Helixi86 – 100Combined sources15
Beta strandi130 – 137Combined sources8
Helixi140 – 142Combined sources3
Helixi147 – 150Combined sources4
Beta strandi162 – 167Combined sources6
Helixi172 – 182Combined sources11
Beta strandi189 – 199Combined sources11
Helixi206 – 213Combined sources8
Beta strandi217 – 229Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0DX-ray2.50A5-101[»]
2NA1NMR-A121-235[»]
3RPGX-ray2.65B1-109[»]
4R8PX-ray3.28K/M2-109[»]
5FR6X-ray2.51A121-235[»]
ProteinModelPortaliP35226.
SMRiP35226.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35226.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 228Interaction with E4F11 PublicationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 95Nuclear localization signalSequence analysisAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi251 – 326Pro/Ser-richAdd BLAST76

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP35226.
KOiK11459.
OrthoDBiEOG090A08NT.
PhylomeDBiP35226.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF16207. RAWUL. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P35226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS
60 70 80 90 100
KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA
110 120 130 140 150
HPSADAANGS NEDRGEVADE DKRIITDDEI ISLSIEFFDQ NRLDRKVNKD
160 170 180 190 200
KEKSKEEVND KRYLRCPAAM TVMHLRKFLR SKMDIPNTFQ IDVMYEEEPL
210 220 230 240 250
KDYYTLMDIA YIYTWRRNGP LPLKYRVRPT CKRMKISHQR DGLTNAGELE
260 270 280 290 300
SDSGSDKANS PAGGIPSTSS CLPSPSTPVQ SPHPQFPHIS STMNGTSNSP
310 320
SGNHQSSFAN RPRKSSVNGS SATSSG
Length:326
Mass (Da):36,949
Last modified:August 13, 2002 - v2
Checksum:i030A7D396BADA543
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109G → S in AAB27059 (PubMed:8390036).Curated1
Sequence conflicti265I → V in AAA19873 (PubMed:8268912).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05208718C → Y. Corresponds to variant dbSNP:rs1042059Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13689 mRNA. Translation: AAA19873.1.
AK313235 mRNA. Translation: BAG36046.1.
AL158211 Genomic DNA. Translation: CAI15958.1.
CH471072 Genomic DNA. Translation: EAW86148.1.
CH471072 Genomic DNA. Translation: EAW86150.1.
CH471072 Genomic DNA. Translation: EAW86151.1.
CH471072 Genomic DNA. Translation: EAW86154.1.
BC011652 mRNA. Translation: AAH11652.1.
AH004292 mRNA. Translation: AAB27059.1.
CCDSiCCDS7138.1.
PIRiI54339.
RefSeqiNP_001190991.1. NM_001204062.1.
NP_005171.4. NM_005180.8.
UniGeneiHs.380403.
Hs.731287.

Genome annotation databases

EnsembliENST00000376663; ENSP00000365851; ENSG00000168283.
GeneIDi100532731.
648.
KEGGihsa:100532731.
hsa:648.
UCSCiuc001irh.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13689 mRNA. Translation: AAA19873.1.
AK313235 mRNA. Translation: BAG36046.1.
AL158211 Genomic DNA. Translation: CAI15958.1.
CH471072 Genomic DNA. Translation: EAW86148.1.
CH471072 Genomic DNA. Translation: EAW86150.1.
CH471072 Genomic DNA. Translation: EAW86151.1.
CH471072 Genomic DNA. Translation: EAW86154.1.
BC011652 mRNA. Translation: AAH11652.1.
AH004292 mRNA. Translation: AAB27059.1.
CCDSiCCDS7138.1.
PIRiI54339.
RefSeqiNP_001190991.1. NM_001204062.1.
NP_005171.4. NM_005180.8.
UniGeneiHs.380403.
Hs.731287.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H0DX-ray2.50A5-101[»]
2NA1NMR-A121-235[»]
3RPGX-ray2.65B1-109[»]
4R8PX-ray3.28K/M2-109[»]
5FR6X-ray2.51A121-235[»]
ProteinModelPortaliP35226.
SMRiP35226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107116. 370 interactors.
1529410. 5 interactors.
DIPiDIP-41879N.
IntActiP35226. 46 interactors.
MINTiMINT-158260.
STRINGi9606.ENSP00000365851.

PTM databases

iPTMnetiP35226.
PhosphoSitePlusiP35226.

Polymorphism and mutation databases

BioMutaiBMI1.
DMDMi22258801.

Proteomic databases

EPDiP35226.
MaxQBiP35226.
PaxDbiP35226.
PeptideAtlasiP35226.
PRIDEiP35226.

Protocols and materials databases

DNASUi648.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000376663; ENSP00000365851; ENSG00000168283.
GeneIDi100532731.
648.
KEGGihsa:100532731.
hsa:648.
UCSCiuc001irh.4. human.

Organism-specific databases

CTDi100532731.
648.
DisGeNETi648.
GeneCardsiBMI1.
HGNCiHGNC:1066. BMI1.
HPAiCAB011120.
HPA030471.
HPA030472.
MIMi164831. gene.
neXtProtiNX_P35226.
OpenTargetsiENSG00000168283.
PharmGKBiPA25376.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP35226.
KOiK11459.
OrthoDBiEOG090A08NT.
PhylomeDBiP35226.
TreeFamiTF324206.

Enzyme and pathway databases

ReactomeiR-HSA-2559580. Oxidative Stress Induced Senescence.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-4570464. SUMOylation of RNA binding proteins.
SIGNORiP35226.

Miscellaneous databases

EvolutionaryTraceiP35226.
GeneWikiiBMI1.
PROiP35226.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168283.
CleanExiHS_BMI1.
ExpressionAtlasiP35226. baseline and differential.
GenevisibleiP35226. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF16207. RAWUL. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBMI1_HUMAN
AccessioniPrimary (citable) accession number: P35226
Secondary accession number(s): Q16030, Q5T8Z3, Q96F37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: August 13, 2002
Last modified: February 15, 2017
This is version 178 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The hPRC-H complex purification reported by PubMed:12167701 probably presents a mixture of different PRC1-like complexes.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.