P35225 (IL13_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-13
Short name=IL-13

Gene names

Name:	IL13
Synonyms:	NC30

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses.
Subunit structure	Interacts with IL13RA2.
Subcellular location	Secreted.
Involvement in disease	Allergic rhinitis (ALRH) [MIM:607154]: A common disease with complex inheritance characterized by mucosal inflammation caused by allergen exposure. Note: Disease susceptibility is associated with variations affecting the gene represented in this entry.
Sequence similarities	Belongs to the IL-4/IL-13 family.
Caution	It is uncertain whether Met-1 or Met-15 is the initiator.
Sequence caution	The sequence AAA36107.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAA83738.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAB01681.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAC03535.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAK53823.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA48824.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Inferred by curator PubMed 10725748. Source: UniProtKB cellular component movement Traceable author statement PubMed 10725748. Source: ProtInc cellular response to cytokine stimulus Inferred from electronic annotation. Source: Compara cellular response to mechanical stimulus Inferred from electronic annotation. Source: Compara immune response Inferred from electronic annotation. Source: InterPro inflammatory response Traceable author statement Ref.1. Source: ProtInc negative regulation of lung ciliated cell differentiation Non-traceable author statement PubMed 20539013. Source: BHF-UCL negative regulation of transforming growth factor beta production Inferred from electronic annotation. Source: Compara positive regulation of B cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of connective tissue growth factor production Inferred from electronic annotation. Source: Compara positive regulation of immunoglobulin production Inferred from electronic annotation. Source: Compara positive regulation of lung goblet cell differentiation Non-traceable author statement PubMed 20539013. Source: BHF-UCL positive regulation of macrophage activation Inferred from electronic annotation. Source: Compara positive regulation of pancreatic stellate cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of protein secretion Inferred from electronic annotation. Source: Compara positive regulation of release of sequestered calcium ion into cytosol Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of tyrosine phosphorylation of Stat6 protein Inferred from electronic annotation. Source: Compara regulation of proton transport Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to nicotine Inferred from electronic annotation. Source: Compara signal transduction Non-traceable author statement PubMed 10725748. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from electronic annotation. Source: Compara extracellular space Inferred from sequence or structural similarity PubMed 16275384. Source: UniProtKB
Molecular_function	cytokine activity Traceable author statement PubMed 10725748. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IL13RA1	P78552	4	EBI-1647828,EBI-1391535

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 146

122

Interleukin-13

PRO_0000015547

Amino acid modifications

Glycosylation

52

1

N-linked (GlcNAc...) Potential

Glycosylation

63

1

N-linked (GlcNAc...) Potential

Glycosylation

71

1

N-linked (GlcNAc...) Potential

Glycosylation

86

1

N-linked (GlcNAc...) Potential

Disulfide bond

62 ↔ 90

Ref.10 Ref.11

Disulfide bond

78 ↔ 104

Ref.10 Ref.11

Natural variations

Natural variant

144

1

R → Q Associated with increased risk for asthma development; at homozygosity associated with higher levels of serum total IgE in some allergic rhinitis patients. Ref.5 Ref.6 Ref.7 Ref.12 Ref.13 Ref.14
Corresponds to variant rs20541 [ dbSNP | Ensembl ].

VAR_010037

Experimental info

Sequence conflict

1

1

M → R in CAA48824. Ref.2

Sequence conflict

26

1

T → A no nucleotide entry Ref.9

Sequence conflict

32

1

A → D no nucleotide entry Ref.9

Sequence conflict

51

1

V → S no nucleotide entry Ref.9

Sequence conflict

56

1

N → T no nucleotide entry Ref.9

Sequence conflict

59

1

A → R in AAA83738. Ref.3

Sequence conflict

101

1

S → G in AAC03535. Ref.8

Sequence conflict

112

1

Missing in AAA83738. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

146

Helix Strand Turn

Details...

Helix

41 – 54

14

Turn

61 – 64

4

Beta strand

66 – 68

3

Turn

74 – 76

3

Helix

77 – 84

8

Helix

91 – 93

3

Helix

94 – 103

10

Beta strand

104 – 106

3

Beta strand

122 – 124

3

Helix

125 – 142

18

Sequences

	Sequence		Length	Mass (Da)
	P35225 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: BE2D46CCF913D129	FASTA	146	15,816
	10 20 30 40 50 60 MHPLLNPLLL ALGLMALLLT TVIALTCLGG FASPGPVPPS TALRELIEEL VNITQNQKAP 70 80 90 100 110 120 LCNGSMVWSI NLTAGMYCAA LESLINVSGC SAIEKTQRML SGFCPHKVSA GQFSSLHVRD 130 140 TKIEVAQFVK DLLLHLKKLF REGRFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses." Minty A.J., Chalon P., Derocq J.-M., Dumont X., Guillemot J.-C., Kaghad M., Labit C., Leplatois P., Liauzun P., Miloux B., Minty C., Casellas P., Loison G., Lupker J., Shire D., Ferrara P., Caput D. Nature 362:248-250(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function." McKenzie A.N., Culpepper J.A., Waal Malefyt R., Briere F., Punnonen J., Aversa G., Sato A., Dang W., Cocks B.G., Menon S., de Vries J.E., Banchereau J., Zurawski G.R. Proc. Natl. Acad. Sci. U.S.A. 90:3735-3739(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Tandem arrangement of human genes for interleukin-4 and interleukin-13: resemblance in their organization." Smirnov D.V., Smirnova M.G., Korobko V.G., Frolova E.I. Gene 155:277-281(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[4]	"Coexpression of the interleukin-13 and interleukin-4 genes correlates with their physical linkage in the cytokine gene cluster on human chromosome 5q23-31." Dolganov G., Bort S., Lovett M., Burr J., Schubert L., Short D., McGurn M., Gibson C., Lewis D.B. Blood 87:3316-3326(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	SeattleSNPs variation discovery resource Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLN-144.
[6]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLN-144.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-144.
[8]	Jang J.S., Kim B.E. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-146.
[9]	"The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genes." Morgan J.G., Dolganov G.M., Robbins S.E., Hinton L.M., Lovett M. Nucleic Acids Res. 20:5173-5179(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-63. Tissue: Peripheral blood lymphocyte.
[10]	"Solution structure of human IL-13 and implication for receptor binding." Moy F.J., Diblasio E., Wilhelm J., Powers R. J. Mol. Biol. 310:219-230(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 35-146, DISULFIDE BONDS.
[11]	"Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2." Lupardus P.J., Birnbaum M.E., Garcia K.C. Structure 18:332-342(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 15-146 IN COMPLEX WITH IL13RA2, DISULFIDE BONDS.
[12]	"Genetic variants of IL-13 signalling and human asthma and atopy." Heinzmann A., Mao X.-Q., Akaiwa M., Kreomer R.T., Gao P.-S., Ohshima K., Umeshita R., Abe Y., Braun S., Yamashita T., Roberts M.H., Sugimoto R., Arima K., Arinobu Y., Yu B., Kruse S., Enomoto T., Dake Y. , Kawai M., Shimazu S., Sasaki S., Adra C.N., Kitaichi M., Inoue H., Yamauchi K., Tomichi N., Kurimoto F., Hamasaki N., Hopkin J.M., Izuhara K., Shirakawa T., Deichmann K.A. Hum. Mol. Genet. 9:549-559(2000) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT GLN-144.
[13]	"Identification and association of polymorphisms in the interleukin-13 gene with asthma and atopy in a Dutch population." Howard T.D., Whittaker P.A., Zaiman A.L., Koppelman G.H., Xu J., Hanley M.T., Meyers D.A., Postma D.S., Bleecker E.R. Am. J. Respir. Cell Mol. Biol. 25:377-384(2001) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT GLN-144.
[14]	"A common IL-13 Arg130Gln single nucleotide polymorphism among Chinese atopy patients with allergic rhinitis." Wang M., Xing Z.-M., Lu C., Ma Y.-X., Yu D.-L., Yan Z., Wang S.-W., Yu L.-S. Hum. Genet. 113:387-390(2003) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT GLN-144.
+	Additional computationally mapped references.

Web resources

Wikipedia

Interleukin-13 entry

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X69079 mRNA. Translation: CAA48823.1.
X69079 mRNA. Translation: CAA48824.1. Different initiation.
L06801 mRNA. Translation: AAA36107.1. Different initiation.
U10307 Genomic DNA. Translation: AAA83738.1. Different initiation.
U31120 Genomic DNA. Translation: AAB01681.1. Different initiation.
AF377331 Genomic DNA. Translation: AAK53823.1. Different initiation.
AC004039 Genomic DNA. No translation available.
BC096139 mRNA. Translation: AAH96139.1.
AF043334 mRNA. Translation: AAC03535.1. Different initiation.

IPI

IPI00807607.

PIR

A47481. I38060.

RefSeq

NP_002179.2. NM_002188.2.

UniGene

Hs.845.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GA3	NMR	-	A	35-146	[»]
1IJZ	NMR	-	A	35-146	[»]
1IK0	NMR	-	A	35-146	[»]
1J9U	model	-	A	38-146	[»]
3BPO	X-ray	3.00	A	34-146	[»]
3G6D	X-ray	3.20	A	35-146	[»]
3ITR	model	-	A	38-146	[»]
3ITS	model	-	A	38-146	[»]
3L5W	X-ray	2.00	I/J	35-146	[»]
3L5X	X-ray	1.90	A	35-146	[»]
3L5Y	X-ray	2.80	A	35-146	[»]
3LB6	X-ray	3.05	A/B	15-146	[»]
4I77	X-ray	1.90	Z	35-146	[»]

ProteinModelPortal

P35225.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3224N.

IntAct

P35225. 1 interaction.

STRING

9606.ENSP00000304915.

Polymorphism databases

DMDM

239938644.

Proteomic databases

PRIDE

P35225.

Protocols and materials databases

DNASU

3596.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000304506; ENSP00000304915; ENSG00000169194.

GeneID

3596.

KEGG

hsa:3596.

Organism-specific databases

CTD

3596.

GeneCards

GC05P131991.

H-InvDB

HIX0031957.

HGNC

HGNC:5973. IL13.

HPA

HPA042421.

MIM

147683. gene.
607154. phenotype.

neXtProt

NX_P35225.

PharmGKB

PA199.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG42350.

HOGENOM

HOG000113028.

HOVERGEN

HBG006140.

InParanoid

P35225.

KO

K05435.

OrthoDB

EOG437RGJ.

Enzyme and pathway databases

Pathway_Interaction_DB

il12_stat4pathway. IL12 signaling mediated by STAT4.

Gene expression databases

ArrayExpress

P35225.

Bgee

P35225.

CleanEx

HS_IL13.

Genevestigator

P35225.

GermOnline

ENSG00000169194. Homo sapiens.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR020470. Interleukin-13.
IPR018096. Interleukin-4/-13_CS.
IPR001325. Interleukin-4/13.
[Graphical view]

PANTHER

PTHR11322. PTHR11322. 1 hit.

Pfam

PF03487. IL13. 1 hit.
[Graphical view]

PRINTS

PR01929. INTRLEUKIN13.

SMART

SM00190. IL4_13. 1 hit.
[Graphical view]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

PROSITE

PS00838. INTERLEUKIN_4_13. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

IL13. human.

EvolutionaryTrace

P35225.

GenomeRNAi

3596.

NextBio

14053.

SOURCE

Search...

Entry information

Entry name

IL13_HUMAN

Accession

Primary (citable) accession number: P35225
Secondary accession number(s): O43644, Q4VB52, Q9UDC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 143 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.