P35213 (1433B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein beta/alpha

Alternative name(s):
Prepronerve growth factor RNH-1
Protein kinase C inhibitor protein 1
Short name=KCIP-1

Cleaved into the following chain:

1. 14-3-3 protein beta/alpha, N-terminally processed

Gene names

Name:

Ywhab

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2 By similarity.
Subunit structure	Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1 (phosphorylated form); the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2, YAP1 and PRKCE (phosphorylated form). Interacts with SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2 By similarity. Ref.5
Subcellular location	Cytoplasm. Melanosome By similarity.
Post-translational modification	The alpha, brain-specific form differs from the beta form in being phosphorylated By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion By similarity. Isoform Short contains a N-acetylmethionine at position 1 By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative initiation
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic sequestering of protein Inferred from electronic annotation. Source: Compara negative regulation of protein dephosphorylation Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 18460465. Source: RGD positive regulation of catalytic activity Inferred from electronic annotation. Source: Compara protein heterooligomerization Inferred from physical interaction PubMed 12786973. Source: RGD protein targeting Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara transcriptional repressor complex Inferred from direct assay PubMed 18460465. Source: RGD
Molecular_function	transcription corepressor activity Inferred from mutant phenotype PubMed 18460465. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P35213-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P35213-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: No experimental confirmation available. Contains a N-acetylmethionine at position 1 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	14-3-3 protein beta/alpha		PRO_0000367904
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 246	245	14-3-3 protein beta/alpha, N-terminally processed		PRO_0000000007
Sites
	Site	58	1	Interaction with phosphoserine on interacting protein By similarity
	Site	129	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	2	1	N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processed By similarity
	Modified residue	60	1	Phosphoserine By similarity
	Modified residue	70	1	N6-acetyllysine By similarity
	Modified residue	117	1	N6-acetyllysine By similarity
	Modified residue	186	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 2	2	Missing in isoform Short.		VSP_018635

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B663D1F2182F9C43	FASTA	246	28,054
	10 20 30 40 50 60 MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS 70 80 90 100 110 120 WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICSDVL ELLDKYLILN ATHAESKVFY 130 140 150 160 170 180 LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY 190 200 210 220 230 240 YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
	Isoform Short [UniParc]. Checksum: 138821F2035AE27B	FASTA	244	27,822
	10 20 30 40 50 60 MDKSELVQKA KLAEQAERYD DMAAAMKAVT EQGHELSNEE RNLLSVAYKN VVGARRSSWR 70 80 90 100 110 120 VISSIEQKTE RNEKKQQMGK EYREKIEAEL QDICSDVLEL LDKYLILNAT HAESKVFYLK 130 140 150 160 170 180 MKGDYFRYLS EVASGDNKQT TVSNSQQAYQ EAFEISKKEM QPTHPIRLGL ALNFSVFYYE 190 200 210 220 230 240 ILNSPEKACS LAKTAFDEAI AELDTLNEES YKDSTLIMQL LRDNLTLWTS ENQGDEGDAG EGEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of rat cDNAs for beta and gamma subtypes of 14-3-3 protein and developmental changes in expression of their mRNAs in the nervous system." Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H. Brain Res. Mol. Brain Res. 17:135-146(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"Function of RNH-1/14-3-3 beta gene in cellular differentiation and proliferation." Takai R., Tanaka E., Miyazaki T., Suda M., Tashiro F. J. Biochem. 118:1045-1053(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	Lubec G., Kang S.U., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 14-57; 63-70; 86-117; 130-159; 161-189 AND 196-224, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
[5]	"Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis." Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C. Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-43, INTERACTION WITH AANAT, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17446 mRNA. Translation: BAA04260.1. S55223 mRNA. Translation: AAA13843.1. S83440 mRNA. Translation: AAB50874.1. BC076502 mRNA. Translation: AAH76502.1.
IPI	IPI00230837. IPI00760126.
PIR	A49023.
RefSeq	NP_062250.1. NM_019377.1.
UniGene	Rn.8653.
3D structure databases
ProteinModelPortal	P35213.
SMR	P35213. Positions 3-233.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35213. 1 interaction.
MINT	MINT-270245.
STRING	10116.ENSRNOP00000016981.
PTM databases
PhosphoSite	P35213.
Proteomic databases
PaxDb	P35213.
PRIDE	P35213.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016981; ENSRNOP00000016981; ENSRNOG00000010945.
GeneID	56011.
KEGG	rno:56011.
UCSC	RGD:61998. rat.
Organism-specific databases
CTD	7529.
RGD	61998. Ywhab.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101832.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P35213.
KO	K16197.
OMA	CNDVLXT.
OrthoDB	EOG4N30PR.
Enzyme and pathway databases
Reactome	REACT_111984. Signal Transduction.
Gene expression databases
Genevestigator	P35213.
GermOnline	ENSRNOG00000010945. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	611079.

Entry information

Entry name

1433B_RAT

Accession

Primary (citable) accession number: P35213

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 121 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program