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Protein

14-3-3 protein beta/alpha

Gene

Ywhab

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD
  • transcription corepressor activity Source: RGD

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-165159. mTOR signalling.
R-RNO-166208. mTORC1-mediated signalling.
R-RNO-170968. Frs2-mediated activation.
R-RNO-170984. ARMS-mediated activation.
R-RNO-2028269. Signaling by Hippo.
R-RNO-392517. Rap1 signalling.
R-RNO-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-5673000. RAF activation.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein beta/alpha
Alternative name(s):
Prepronerve growth factor RNH-1
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Cleaved into the following chain:
Gene namesi
Name:Ywhab
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi61998. Ywhab.

Subcellular locationi

  • Cytoplasm By similarity
  • Melanosome By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: Reactome
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: Ensembl
  • nucleus Source: RGD
  • perinuclear region of cytoplasm Source: Ensembl
  • protein complex Source: RGD
  • transcriptional repressor complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24624614-3-3 protein beta/alphaPRO_0000367904Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 24624514-3-3 protein beta/alpha, N-terminally processedPRO_0000000007Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity
Modified residuei2 – 21PhosphothreonineBy similarity
Modified residuei60 – 601PhosphoserineBy similarity
Modified residuei70 – 701N6-acetyllysineBy similarity
Modified residuei84 – 841Nitrated tyrosineBy similarity
Modified residuei106 – 1061Nitrated tyrosineBy similarity
Modified residuei117 – 1171N6-acetyllysineBy similarity
Modified residuei186 – 1861PhosphoserineBy similarity
Modified residuei232 – 2321PhosphoserineBy similarity
Isoform Short (identifier: P35213-2)
Modified residuei1 – 11N-acetylmethionine By similarityBy similarity

Post-translational modificationi

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity
Isoform Short contains a N-acetylmethionine at position 1.By similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP35213.
PRIDEiP35213.

PTM databases

iPTMnetiP35213.
PhosphoSiteiP35213.

Expressioni

Gene expression databases

GenevisibleiP35213. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2 (By similarity). Interacts with PKA-phosphorylated AANAT (PubMed:11427721). Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 581Interaction with phosphoserine on interacting proteinBy similarity
Sitei129 – 1291Interaction with phosphoserine on interacting proteinBy similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
VimP310003EBI-917309,EBI-368510

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi248554. 7 interactions.
IntActiP35213. 6 interactions.
MINTiMINT-270245.
STRINGi10116.ENSRNOP00000016981.

Structurei

3D structure databases

ProteinModelPortaliP35213.
SMRiP35213. Positions 3-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP35213.
KOiK16197.
OMAiMGREYRE.
OrthoDBiEOG7HHWT3.
PhylomeDBiP35213.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P35213-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY
60 70 80 90 100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICSDVL
110 120 130 140 150
ELLDKYLILN ATHAESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA
160 170 180 190 200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE
210 220 230 240
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Length:246
Mass (Da):28,054
Last modified:January 23, 2007 - v3
Checksum:iB663D1F2182F9C43
GO
Isoform Short (identifier: P35213-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: No experimental confirmation available.By similarity
Show »
Length:244
Mass (Da):27,822
Checksum:i138821F2035AE27B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 22Missing in isoform Short. CuratedVSP_018635

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17446 mRNA. Translation: BAA04260.1.
S55223 mRNA. Translation: AAA13843.1.
S83440 mRNA. Translation: AAB50874.1.
BC076502 mRNA. Translation: AAH76502.1.
PIRiA49023.
RefSeqiNP_062250.1. NM_019377.1. [P35213-1]
UniGeneiRn.8653.

Genome annotation databases

EnsembliENSRNOT00000016981; ENSRNOP00000016981; ENSRNOG00000010945. [P35213-1]
GeneIDi56011.
KEGGirno:56011.
UCSCiRGD:61998. rat. [P35213-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17446 mRNA. Translation: BAA04260.1.
S55223 mRNA. Translation: AAA13843.1.
S83440 mRNA. Translation: AAB50874.1.
BC076502 mRNA. Translation: AAH76502.1.
PIRiA49023.
RefSeqiNP_062250.1. NM_019377.1. [P35213-1]
UniGeneiRn.8653.

3D structure databases

ProteinModelPortaliP35213.
SMRiP35213. Positions 3-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248554. 7 interactions.
IntActiP35213. 6 interactions.
MINTiMINT-270245.
STRINGi10116.ENSRNOP00000016981.

PTM databases

iPTMnetiP35213.
PhosphoSiteiP35213.

Proteomic databases

PaxDbiP35213.
PRIDEiP35213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016981; ENSRNOP00000016981; ENSRNOG00000010945. [P35213-1]
GeneIDi56011.
KEGGirno:56011.
UCSCiRGD:61998. rat. [P35213-1]

Organism-specific databases

CTDi7529.
RGDi61998. Ywhab.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP35213.
KOiK16197.
OMAiMGREYRE.
OrthoDBiEOG7HHWT3.
PhylomeDBiP35213.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-165159. mTOR signalling.
R-RNO-166208. mTORC1-mediated signalling.
R-RNO-170968. Frs2-mediated activation.
R-RNO-170984. ARMS-mediated activation.
R-RNO-2028269. Signaling by Hippo.
R-RNO-392517. Rap1 signalling.
R-RNO-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-5673000. RAF activation.
R-RNO-5674135. MAP2K and MAPK activation.
R-RNO-5675221. Negative regulation of MAPK pathway.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiP35213.

Gene expression databases

GenevisibleiP35213. RN.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of rat cDNAs for beta and gamma subtypes of 14-3-3 protein and developmental changes in expression of their mRNAs in the nervous system."
    Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H.
    Brain Res. Mol. Brain Res. 17:135-146(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "Function of RNH-1/14-3-3 beta gene in cellular differentiation and proliferation."
    Takai R., Tanaka E., Miyazaki T., Suda M., Tashiro F.
    J. Biochem. 118:1045-1053(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. Lubec G., Kang S.U., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 14-57; 63-70; 86-117; 130-159; 161-189 AND 196-224, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.
  5. "Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis."
    Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C.
    Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-43, INTERACTION WITH AANAT, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry namei1433B_RAT
AccessioniPrimary (citable) accession number: P35213
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.