Reviewed, UniProtKB/Swiss-Prot P35211 (ORYZ_ASPFL)

Names and origin

Protein names

Recommended name:
    Oryzin
    EC=3.4.21.63
Alternative name(s):
    Alkaline proteinase
      Short name=ALP
    Elastase
    Elastinolytic serine proteinase

Gene names

Name:	alk1
Synonyms:	alp

Organism

Aspergillus flavus

Taxonomic identifier

5059 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Elastolytic enzyme probably acting as a virulence factor in invasive aspergillosis.
Catalytic activity	Hydrolysis of proteins with broad specificity, and of Bz-Arg-OEt > Ac-Tyr-OEt. Does not hydrolyze peptide amides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S8 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	identical protein binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 121	100			PRO_0000027080
	Chain	122 – 403	282	Oryzin		PRO_0000027081
Sites
	Active site	162	1	Charge relay system By similarity
	Active site	193	1	Charge relay system By similarity
	Active site	349	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Glycosylation	309	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P35211-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: D9E86FFBA26107C6	FASTA	403	42,769
	10 20 30 40 50 60 MQSIKRTLLL LGAVLPAVLA GPIFPHRRAP TTIPGKYIVT FKSDVDQAAI DKHTAWATDI 70 80 90 100 110 120 HKRNLQRRDS SEEDLPIGIE RNFKINKFAA YSGSFDEDTI AQIRQSDEVA AVEEDQVWHL 130 140 150 160 170 180 FDLTTQSDAP WGLGSISHKG QPSTDYIYDT NGGEGTYAYV VDIGINVDHE EFEGRASLAY 190 200 210 220 230 240 HAAGGQHVDG VGHGTHVSGT IGGKTYGVAK KANLLSVKVF VGESSSTSII LDGFNWAAND 250 260 270 280 290 300 IVSKKRTGKA AINMSLGGGY SKAFNDAVEN AFNEGVLSIV AAGNENTDAS RTSPASAPDA 310 320 330 340 350 360 FTVAAINVNN TRAYFSNYGS VVDIFAPGQN ILSAWIGSNT ATNTISGTSM ATPHIVGLSI 370 380 390 400 YLMSLEVLSS PKAVSDRIKE LATRGVVSNV AGSPNLLAYN GNA

References

[1]

"Isolation, characterization, and cloning of cDNA and the gene for an elastinolytic serine proteinase from Aspergillus flavus." Ramesh M.V., Sirakova T., Kolattukudy P.E. Infect. Immun. 62:79-85(1994) [PubMed: 8262652] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08473 Genomic DNA. Translation: AAA32691.1. S67840 Genomic DNA. Translation: AAB29384.1.
3D structure databases
HSSP	HSSP built from PDB template 1IC6 based on UniProtKB P06873.
ModBase	Search...
Protein family/group databases
MEROPS	S08.053.
Enzyme and pathway databases
BRENDA	3.4.21.63. 2238.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR010259. Prot_inh_S8A. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF05922. Inhibitor_I9. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. False negative. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORYZ_ASPFL

Accession

Primary (citable) accession number: P35211

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)