P35210 (SPT23_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SPT23

Gene names

Name:	SPT23
Ordered Locus Names:	YKL020C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1082 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dosage-dependent suppressor of Ty-induced promoter mutations. May exert its suppression effect through protein-protein interactions since does not present any of the motifs generally found in transcriptional activators or DNA binding proteins.
Miscellaneous	Present with 432 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 ANK repeats. Contains 1 IPT/TIG domain.
Sequence caution	The sequence AAA20575.1 differs from that shown. Reason: Frameshift at position 738.

Ontologies

Keywords
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cold Inferred from genetic interaction PubMed 11855848. Source: SGD chromatin silencing at silent mating-type cassette Inferred from genetic interaction PubMed 11063674. Source: SGD positive regulation of unsaturated fatty acid biosynthetic process by positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 9927444. Source: SGD
Cellular_component	integral to endoplasmic reticulum membrane Inferred from sequence model PubMed 11007476. Source: SGD nucleus Inferred from direct assay PubMed 11007476. Source: SGD
Molecular_function	DNA binding Inferred from physical interaction PubMed 15343339. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1082	1082	Protein SPT23		PRO_0000067076
Regions
	Domain	508 – 585	78	IPT/TIG
	Repeat	709 – 738	30	ANK 1
	Repeat	742 – 771	30	ANK 2
Amino acid modifications
	Modified residue	66	1	Phosphothreonine Ref.6
	Modified residue	595	1	Phosphoserine Ref.5
	Modified residue	827	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	715	1	H → P in AAA20575. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P35210 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 620C688ECC0ECBD7	FASTA	1,082	121,338
	10 20 30 40 50 60 MMSGTGNVSS MLHSYSANIQ HNDGSPDLDL LESELLDIAL LNSGSSLQDP GLLSLNQEKM 70 80 90 100 110 120 ITAGTTTPGK EDEGELRDDI ASLQGLLDRH VQFGRKLPLR TPYANPLDFI NINPQSLPLS 130 140 150 160 170 180 LEIIGLPKVS RVETQMKLSF RIRNAHARKN FFIHLPSDCI AKDKFFTSSD DPTNLTIPNR 190 200 210 220 230 240 DINERTLFLD AFLLCASNNN SNNFKQTYVC NRCINREKRR ASRRKSGLND NSIWQNNENK 250 260 270 280 290 300 RAIIFNSKQL FIISNNGLSG NSNCINFDLP TRIVCYCRHH KATNGFVVLF LLRDHNGDIL 310 320 330 340 350 360 AKTITDPIMI MDKKNASNTT TPTSTSNAQV SPMTNDTRSF SSPQSDLNFP SEFPLPSNSK 370 380 390 400 410 420 NFVISTNCML DSNCNNNNND NDNKNNIKTN TAMMNNNRHF PSPNSSSEDS NHSFSDIHFS 430 440 450 460 470 480 NNNDNNLHRS LDSWSSTGFN SSSNPALTTL TSDFSAASAR HTGKRQRSVN EPFMSTPNTF 490 500 510 520 530 540 SRLPQKFIDS SKDISNHNSV PVALNNKPSI QRVIPAQGSI NGGIEVTLLG SKFKQGLIIK 550 560 570 580 590 600 FGENIALSSQ CWNESTMVTY LPPSSKPGPV LVTIVDPSET SMRNNSNSSV STSNSTNDIL 610 620 630 640 650 660 HLNKYTGEKA IFTYVDDTDR QLIELALQIV GLKMNGKLED ARNIAKRIVG SDSSPSNNNA 670 680 690 700 710 720 GLHSQNSSLN SYTNMMRNIN DEQLITEVIK SFKRNNNLST VNLSMCDVRG RTLLHLAAFN 730 740 750 760 770 780 NWYSLVSLLI KYGSHLNDQD LFGFTPLHMA CINGDLRIIR LLLECNVNIM KKTRNGFIAK 790 800 810 820 830 840 QFFLMNYTVN KTRYSNYETS LFDDILTRLT KNTTGSSDTQ PFERNVSQSS FNSSLFDDDD 850 860 870 880 890 900 ADHDYVQERK YLLADSAALA PEQSNCNDNT SFSILDSDSG YDISDCESSS DEIALEFFNT 910 920 930 940 950 960 HKIKDFSSKP NEIPKTTKTS IEPDGSLWNR MLTRLNDELP KYEDLFPKKP KNWELGSKSV 970 980 990 1000 1010 1020 EIGPDNSAQM TVDDSQTSSE DDELEALQVG FNTIFSKKQN FQNDKMLLFF WIPLTLVLLL 1030 1040 1050 1060 1070 1080 CFTLSNLGKD DDMFHNLSKI VQEYLRIGLA KVLLGNERMK TSFKMQLSKF QNNNILNDMR VN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Molecular characterization of the SPT23 gene: a dosage-dependent suppressor of Ty-induced promoter mutations from Saccharomyces cerevisiae." Burkett T.J., Garfinkel D.J. Yeast 10:81-92(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 136-752.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-595 AND SER-827, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-66, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28020 Genomic DNA. Translation: CAA81855.1. L24760 Genomic DNA. Translation: AAA20575.1. Frameshift. BK006944 Genomic DNA. Translation: DAA09135.1.
PIR	S37837.
RefSeq	NP_012905.1. NM_001179586.1.
3D structure databases
ProteinModelPortal	P35210.
SMR	P35210. Positions 710-776.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1307N.
IntAct	P35210. 5 interactions.
MINT	MINT-400551.
STRING	4932.YKL020C.
Proteomic databases
PaxDb	P35210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL020C; YKL020C; YKL020C.
GeneID	853848.
KEGG	sce:YKL020C.
Organism-specific databases
CYGD	YKL020c.
SGD	S000001503. SPT23.
Phylogenomic databases
eggNOG	COG0666.
GeneTree	ENSGT00530000067551.
HOGENOM	HOG000113576.
KO	K06867.
OrthoDB	EOG4Q8863.
Gene expression databases
Genevestigator	P35210.
GermOnline	YKL020C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.40.20. 1 hit. 2.60.40.10. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002909. IPT_TIG_rcpt. [Graphical view]
Pfam	PF00023. Ank. 2 hits. PF01833. TIG. 1 hit. [Graphical view]
SMART	SM00248. ANK. 2 hits. SM00429. IPT. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF81296. Ig_E-set. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	975073.

Entry information

Entry name

SPT23_YEAST

Accession

Primary (citable) accession number: P35210
Secondary accession number(s): D6VXR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program