P35208 (SPT10_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SPT10

Gene names

Name:	SPT10
Synonyms:	CRE1, SUD1
Ordered Locus Names:	YJL127C
ORF Names:	J0702

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for normal transcription at a number of loci in yeast. Affects transcription at Ty1 elements, at PHO5, STE6 and ADH2.
Miscellaneous	Present with 1500 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from mutant phenotype PubMed 17078097. Source: SGD chromatin remodeling Traceable author statement PubMed 8007957. Source: SGD chromatin silencing at rDNA Inferred from mutant phenotype PubMed 21057056. Source: SGD chromatin silencing at silent mating-type cassette Inferred from genetic interaction PubMed 21057056. Source: SGD chromatin silencing at telomere Inferred from mutant phenotype PubMed 21057056. Source: SGD negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 17526727. Source: SGD
Cellular_component	nucleus Traceable author statement Ref.5. Source: SGD
Molecular_function	histone acetyltransferase activity Inferred from direct assay PubMed 12192040. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 16199888. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Protein SPT10		PRO_0000072160
Regions
	Domain	121 – 259	139	N-acetyltransferase
Experimental info
	Mutagenesis	388	1	C → S: Loss of complementation of the suppression phenotype.

Sequences

	Sequence		Length	Mass (Da)
	P35208 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 9E81C87E2A66D6C5	FASTA	640	72,922
	10 20 30 40 50 60 MLNQHTSSVP DDEHLQMAHQ NSSSEVRNEA AVPDQLLTPL QPYTILLKDG ETIATMYPIP 70 80 90 100 110 120 AYPDLLPLGL LNFLLDEFNM EVEKGDSFPY YETLSLEEFK NVWFHNDGHV CIMVLGEIPE 130 140 150 160 170 180 LDYSMDTEAD TNDNFGTEIE TTKHTTQYKK RKERRNLNLS MQWEKQCLGI FDLKPAYPGR 190 200 210 220 230 240 SAHVVTGTFL VNAGIRGKGI GKTLMETFIE WSKKLGFTSS FFPLIYGTNV GIRRILEGLN 250 260 270 280 290 300 FRRIGKLPEA GILKGFDVPV DSFMYGKEFT HITKSIDLLR DPQKSIEIGK YERLKHFLET 310 320 330 340 350 360 GKYPLHCDRN EKARLRVLSK THSVLNGKLM TKGKEIIYDT DQQIQIALEI HLMEHLGINK 370 380 390 400 410 420 VTSKIGEKYH WRGIKSTVSE VISRCQKCKM RYKDGTGVII EQKRAVKQAH MLPTQHIETI 430 440 450 460 470 480 NNPRKSKKHD NALLGQAINF PQNIISSTLN DVEGEPTPPD TNIVQPTFQN ATNSPATTAE 490 500 510 520 530 540 ANEANKRSEF LSSIQSTPLL DDEQSMNSFN RFVEEENSRK RRKYLDVASN GIVPHLTNNE 550 560 570 580 590 600 SQDHANPVNR DERDMNHSVP DLDRNDHTIM NDAMLSLEDN VMAALEMVQK EQQQKINHRG 610 620 630 640 EDVTGQQIDL NNSEGNENSV TKIVNNESNT FTEHNSNIYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SPT10 and SPT21 genes of Saccharomyces cerevisiae." Natsoulis G., Winston F., Boeke J.D. Genetics 136:93-105(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
[2]	"Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon." Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C. Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation and characterization of the SUD1 gene, which encodes a global repressor of core promoter activity in Saccharomyces cerevisiae." Yamashita I. Mol. Gen. Genet. 241:616-626(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-640. Strain: S288c / GRF88.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L24435 Genomic DNA. Translation: AAA35077.1. Z49402 Genomic DNA. Translation: CAA89422.1. X65186 Genomic DNA. Translation: CAA46300.1. BK006943 Genomic DNA. Translation: DAA08675.1.
PIR	S47865.
RefSeq	NP_012408.1. NM_001181560.1.
3D structure databases
ProteinModelPortal	P35208.
SMR	P35208. Positions 168-250.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35208. 32 interactions.
MINT	MINT-4491021.
STRING	4932.YJL127C.
Proteomic databases
PaxDb	P35208.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL127C; YJL127C; YJL127C.
GeneID	853315.
KEGG	sce:YJL127C.
Organism-specific databases
CYGD	YJL127c.
SGD	S000003663. SPT10.
Phylogenomic databases
eggNOG	COG0454.
HOGENOM	HOG000141837.
OrthoDB	EOG4ZCXD9.
Gene expression databases
Genevestigator	P35208.
GermOnline	YJL127C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.630.30. 2 hits.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. IPR015416. Znf_H2C2_histone_UAS-bd. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. PF09337. zf-H2C2. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973657.

Entry information

Entry name

SPT10_YEAST

Accession

Primary (citable) accession number: P35208
Secondary accession number(s): D6VW59

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program