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Protein

Protein SPT10

Gene

SPT10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal transcription at a number of loci in yeast. Affects transcription at Ty1 elements, at PHO5, STE6 and ADH2.

GO - Molecular functioni

  1. histone acetyltransferase activity Source: SGD
  2. sequence-specific DNA binding Source: SGD

GO - Biological processi

  1. chromatin silencing at rDNA Source: SGD
  2. chromatin silencing at silent mating-type cassette Source: SGD
  3. chromatin silencing at telomere Source: SGD
  4. DNA repair Source: SGD
  5. histone acetylation Source: SGD
  6. negative regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31577-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein SPT10
Gene namesi
Name:SPT10
Synonyms:CRE1, SUD1
Ordered Locus Names:YJL127C
ORF Names:J0702
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL127c.
EuPathDBiFungiDB:YJL127C.
SGDiS000003663. SPT10.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi388 – 3881C → S: Loss of complementation of the suppression phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Protein SPT10PRO_0000072160Add
BLAST

Proteomic databases

MaxQBiP35208.
PaxDbiP35208.

Expressioni

Gene expression databases

GenevestigatoriP35208.

Interactioni

Protein-protein interaction databases

BioGridi33629. 369 interactions.
IntActiP35208. 32 interactions.
MINTiMINT-4491021.
STRINGi4932.YJL127C.

Structurei

3D structure databases

SMRiP35208. Positions 168-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 259139N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0454.
HOGENOMiHOG000141837.
InParanoidiP35208.
OrthoDBiEOG7NPG51.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR015416. Znf_H2C2_histone_UAS-bd.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
PF09337. zf-H2C2. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNQHTSSVP DDEHLQMAHQ NSSSEVRNEA AVPDQLLTPL QPYTILLKDG
60 70 80 90 100
ETIATMYPIP AYPDLLPLGL LNFLLDEFNM EVEKGDSFPY YETLSLEEFK
110 120 130 140 150
NVWFHNDGHV CIMVLGEIPE LDYSMDTEAD TNDNFGTEIE TTKHTTQYKK
160 170 180 190 200
RKERRNLNLS MQWEKQCLGI FDLKPAYPGR SAHVVTGTFL VNAGIRGKGI
210 220 230 240 250
GKTLMETFIE WSKKLGFTSS FFPLIYGTNV GIRRILEGLN FRRIGKLPEA
260 270 280 290 300
GILKGFDVPV DSFMYGKEFT HITKSIDLLR DPQKSIEIGK YERLKHFLET
310 320 330 340 350
GKYPLHCDRN EKARLRVLSK THSVLNGKLM TKGKEIIYDT DQQIQIALEI
360 370 380 390 400
HLMEHLGINK VTSKIGEKYH WRGIKSTVSE VISRCQKCKM RYKDGTGVII
410 420 430 440 450
EQKRAVKQAH MLPTQHIETI NNPRKSKKHD NALLGQAINF PQNIISSTLN
460 470 480 490 500
DVEGEPTPPD TNIVQPTFQN ATNSPATTAE ANEANKRSEF LSSIQSTPLL
510 520 530 540 550
DDEQSMNSFN RFVEEENSRK RRKYLDVASN GIVPHLTNNE SQDHANPVNR
560 570 580 590 600
DERDMNHSVP DLDRNDHTIM NDAMLSLEDN VMAALEMVQK EQQQKINHRG
610 620 630 640
EDVTGQQIDL NNSEGNENSV TKIVNNESNT FTEHNSNIYY
Length:640
Mass (Da):72,922
Last modified:February 1, 1994 - v1
Checksum:i9E81C87E2A66D6C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24435 Genomic DNA. Translation: AAA35077.1.
Z49402 Genomic DNA. Translation: CAA89422.1.
X65186 Genomic DNA. Translation: CAA46300.1.
BK006943 Genomic DNA. Translation: DAA08675.1.
PIRiS47865.
RefSeqiNP_012408.1. NM_001181560.1.

Genome annotation databases

EnsemblFungiiYJL127C; YJL127C; YJL127C.
GeneIDi853315.
KEGGisce:YJL127C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24435 Genomic DNA. Translation: AAA35077.1.
Z49402 Genomic DNA. Translation: CAA89422.1.
X65186 Genomic DNA. Translation: CAA46300.1.
BK006943 Genomic DNA. Translation: DAA08675.1.
PIRiS47865.
RefSeqiNP_012408.1. NM_001181560.1.

3D structure databases

SMRiP35208. Positions 168-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33629. 369 interactions.
IntActiP35208. 32 interactions.
MINTiMINT-4491021.
STRINGi4932.YJL127C.

Proteomic databases

MaxQBiP35208.
PaxDbiP35208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL127C; YJL127C; YJL127C.
GeneIDi853315.
KEGGisce:YJL127C.

Organism-specific databases

CYGDiYJL127c.
EuPathDBiFungiDB:YJL127C.
SGDiS000003663. SPT10.

Phylogenomic databases

eggNOGiCOG0454.
HOGENOMiHOG000141837.
InParanoidiP35208.
OrthoDBiEOG7NPG51.

Enzyme and pathway databases

BioCyciYEAST:G3O-31577-MONOMER.

Miscellaneous databases

NextBioi973657.
PROiP35208.

Gene expression databases

GenevestigatoriP35208.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR015416. Znf_H2C2_histone_UAS-bd.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
PF09337. zf-H2C2. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The SPT10 and SPT21 genes of Saccharomyces cerevisiae."
    Natsoulis G., Winston F., Boeke J.D.
    Genetics 136:93-105(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS.
  2. "Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon."
    Cziepluch C., Kordes E., Pujol A., Jauniaux J.-C.
    Yeast 12:1471-1474(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation and characterization of the SUD1 gene, which encodes a global repressor of core promoter activity in Saccharomyces cerevisiae."
    Yamashita I.
    Mol. Gen. Genet. 241:616-626(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 80-640.
    Strain: S288c / GRF88.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPT10_YEAST
AccessioniPrimary (citable) accession number: P35208
Secondary accession number(s): D6VW59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.