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P35206

- CSG2_YEAST

UniProt

P35206 - CSG2_YEAST

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Protein

Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2

Gene
CSG2, CLS2, YBR036C, YBR0404
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for calcium regulation. May regulate calcium accumulation by a non-vacuole organelle. Also regulates the activity of CSH1 and SUR1 during mannosyl phosphorylinositol ceramide synthesis.1 Publication

GO - Molecular functioni

  1. enzyme regulator activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. cellular calcium ion homeostasis Source: SGD
  2. glycosphingolipid biosynthetic process Source: SGD
  3. regulation of catalytic activity Source: GOC
Complete GO annotation...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-695.
YEAST:MONOMER3O-695.

Protein family/group databases

TCDBi2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
Gene namesi
Name:CSG2
Synonyms:CLS2
Ordered Locus Names:YBR036C
ORF Names:YBR0404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

SGDiS000000240. CSG2.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 5033Lumenal Reviewed predictionAdd
BLAST
Transmembranei51 – 7121Helical; Reviewed predictionAdd
BLAST
Topological domaini72 – 14170Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei142 – 16120Helical; Reviewed predictionAdd
BLAST
Topological domaini162 – 1676Lumenal Reviewed prediction
Transmembranei168 – 18720Helical; Reviewed predictionAdd
BLAST
Topological domaini188 – 19710Cytoplasmic Reviewed prediction
Transmembranei198 – 21720Helical; Reviewed predictionAdd
BLAST
Topological domaini218 – 24528Lumenal Reviewed predictionAdd
BLAST
Transmembranei246 – 26520Helical; Reviewed predictionAdd
BLAST
Topological domaini266 – 28520Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei286 – 30520Helical; Reviewed predictionAdd
BLAST
Topological domaini306 – 32419Lumenal Reviewed predictionAdd
BLAST
Transmembranei325 – 34420Helical; Reviewed predictionAdd
BLAST
Topological domaini345 – 35511Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei356 – 37419Helical; Reviewed predictionAdd
BLAST
Topological domaini375 – 38511Lumenal Reviewed predictionAdd
BLAST
Transmembranei386 – 40419Helical; Reviewed predictionAdd
BLAST
Topological domaini405 – 4106Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Chaini18 – 410393Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2PRO_0000021012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi49 – 491N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP35206.

Expressioni

Gene expression databases

GenevestigatoriP35206.

Interactioni

Subunit structurei

Heterodimer of CSH1 and CSG2, and SUR1 and CSG2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSH1P382872EBI-2051140,EBI-20861
SHR3Q027743EBI-2051140,EBI-17099

Protein-protein interaction databases

BioGridi32737. 452 interactions.
DIPiDIP-8095N.
IntActiP35206. 37 interactions.
MINTiMINT-1354206.
STRINGi4932.YBR036C.

Structurei

3D structure databases

ProteinModelPortaliP35206.

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG70202.
HOGENOMiHOG000172600.
OMAiRWEVIGY.
OrthoDBiEOG7JHMFW.

Family and domain databases

InterProiIPR018247. EF_Hand_1_Ca_BS.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35206-1 [UniParc]FASTAAdd to Basket

« Hide

MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS    50
SSLTFYLTFM YFASWLLLVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG 100
SVTNEDVDTF SHVLDDPQPR IPAQQQKQKI ISVATFKYVA KLTVLALIMI 150
VADLTYNMAL SLSPAFDVAL MQNTAIFEIV TLLYGVCGIS RKNYVFRNFL 200
IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL FDRLKGALIC 250
GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL 300
LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK 350
APAEYLTTCN LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS 400
VTLGEGKYHH 410
Length:410
Mass (Da):45,442
Last modified:October 5, 2010 - v2
Checksum:i15780FEBA54E4FD2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → W in AAA34533. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24113 mRNA. Translation: AAA34533.1.
D28120 Genomic DNA. Translation: BAA05666.1.
Z35905 Genomic DNA. Translation: CAA84978.1.
X76078 Genomic DNA. No translation available.
X17441 Genomic DNA. Translation: CAA35491.1.
BK006936 Genomic DNA. Translation: DAA07156.1.
PIRiS45894.
RefSeqiNP_009592.1. NM_001178384.1.

Genome annotation databases

EnsemblFungiiYBR036C; YBR036C; YBR036C.
GeneIDi852324.
KEGGisce:YBR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L24113 mRNA. Translation: AAA34533.1 .
D28120 Genomic DNA. Translation: BAA05666.1 .
Z35905 Genomic DNA. Translation: CAA84978.1 .
X76078 Genomic DNA. No translation available.
X17441 Genomic DNA. Translation: CAA35491.1 .
BK006936 Genomic DNA. Translation: DAA07156.1 .
PIRi S45894.
RefSeqi NP_009592.1. NM_001178384.1.

3D structure databases

ProteinModelPortali P35206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32737. 452 interactions.
DIPi DIP-8095N.
IntActi P35206. 37 interactions.
MINTi MINT-1354206.
STRINGi 4932.YBR036C.

Protein family/group databases

TCDBi 2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbi P35206.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR036C ; YBR036C ; YBR036C .
GeneIDi 852324.
KEGGi sce:YBR036C.

Organism-specific databases

SGDi S000000240. CSG2.

Phylogenomic databases

eggNOGi NOG70202.
HOGENOMi HOG000172600.
OMAi RWEVIGY.
OrthoDBi EOG7JHMFW.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER3O-695.
YEAST:MONOMER3O-695.

Miscellaneous databases

NextBioi 971026.

Gene expression databases

Genevestigatori P35206.

Family and domain databases

InterProi IPR018247. EF_Hand_1_Ca_BS.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae."
    Beeler T.J., Gable K., Zhao C., Dunn T.
    J. Biol. Chem. 269:7279-7284(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast."
    Takita Y., Ohya Y., Anraku Y.
    Mol. Gen. Genet. 246:269-281(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-410.
    Strain: ATCC 204508 / S288c.
  6. "Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene."
    Schulze M., Roedel G.
    Mol. Gen. Genet. 216:37-43(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
  7. "Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p."
    Uemura S., Kihara A., Inokuchi J., Igarashi Y.
    J. Biol. Chem. 278:45049-45055(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiCSG2_YEAST
AccessioniPrimary (citable) accession number: P35206
Secondary accession number(s): D6VQ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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