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Protein

Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2

Gene

CSG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for calcium regulation. May regulate calcium accumulation by a non-vacuole organelle. Also regulates the activity of CSH1 and SUR1 during mannosyl phosphorylinositol ceramide synthesis.1 Publication

GO - Molecular functioni

  1. enzyme regulator activity Source: SGD

GO - Biological processi

  1. cellular calcium ion homeostasis Source: SGD
  2. glycosphingolipid biosynthetic process Source: SGD
  3. regulation of catalytic activity Source: GOC
Complete GO annotation...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-695.
YEAST:MONOMER3O-695.

Protein family/group databases

TCDBi2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
Gene namesi
Name:CSG2
Synonyms:CLS2
Ordered Locus Names:YBR036C
ORF Names:YBR0404
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

SGDiS000000240. CSG2.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 5033LumenalSequence AnalysisAdd
BLAST
Transmembranei51 – 7121HelicalSequence AnalysisAdd
BLAST
Topological domaini72 – 14170CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei142 – 16120HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 1676LumenalSequence Analysis
Transmembranei168 – 18720HelicalSequence AnalysisAdd
BLAST
Topological domaini188 – 19710CytoplasmicSequence Analysis
Transmembranei198 – 21720HelicalSequence AnalysisAdd
BLAST
Topological domaini218 – 24528LumenalSequence AnalysisAdd
BLAST
Transmembranei246 – 26520HelicalSequence AnalysisAdd
BLAST
Topological domaini266 – 28520CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei286 – 30520HelicalSequence AnalysisAdd
BLAST
Topological domaini306 – 32419LumenalSequence AnalysisAdd
BLAST
Transmembranei325 – 34420HelicalSequence AnalysisAdd
BLAST
Topological domaini345 – 35511CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei356 – 37419HelicalSequence AnalysisAdd
BLAST
Topological domaini375 – 38511LumenalSequence AnalysisAdd
BLAST
Transmembranei386 – 40419HelicalSequence AnalysisAdd
BLAST
Topological domaini405 – 4106CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 410393Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2PRO_0000021012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP35206.

Expressioni

Gene expression databases

GenevestigatoriP35206.

Interactioni

Subunit structurei

Heterodimer of CSH1 and CSG2, and SUR1 and CSG2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CSH1P382872EBI-2051140,EBI-20861
SHR3Q027743EBI-2051140,EBI-17099

Protein-protein interaction databases

BioGridi32737. 452 interactions.
DIPiDIP-8095N.
IntActiP35206. 37 interactions.
MINTiMINT-1354206.
STRINGi4932.YBR036C.

Structurei

3D structure databases

ProteinModelPortaliP35206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG70202.
HOGENOMiHOG000172600.
InParanoidiP35206.
OMAiRWEVIGY.
OrthoDBiEOG7JHMFW.

Family and domain databases

InterProiIPR018247. EF_Hand_1_Ca_BS.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35206-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS
60 70 80 90 100
SSLTFYLTFM YFASWLLLVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG
110 120 130 140 150
SVTNEDVDTF SHVLDDPQPR IPAQQQKQKI ISVATFKYVA KLTVLALIMI
160 170 180 190 200
VADLTYNMAL SLSPAFDVAL MQNTAIFEIV TLLYGVCGIS RKNYVFRNFL
210 220 230 240 250
IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL FDRLKGALIC
260 270 280 290 300
GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL
310 320 330 340 350
LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK
360 370 380 390 400
APAEYLTTCN LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS
410
VTLGEGKYHH
Length:410
Mass (Da):45,442
Last modified:October 5, 2010 - v2
Checksum:i15780FEBA54E4FD2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681L → W in AAA34533 (PubMed:8125941).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24113 mRNA. Translation: AAA34533.1.
D28120 Genomic DNA. Translation: BAA05666.1.
Z35905 Genomic DNA. Translation: CAA84978.1.
X76078 Genomic DNA. No translation available.
X17441 Genomic DNA. Translation: CAA35491.1.
BK006936 Genomic DNA. Translation: DAA07156.1.
PIRiS45894.
RefSeqiNP_009592.1. NM_001178384.1.

Genome annotation databases

EnsemblFungiiYBR036C; YBR036C; YBR036C.
GeneIDi852324.
KEGGisce:YBR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L24113 mRNA. Translation: AAA34533.1.
D28120 Genomic DNA. Translation: BAA05666.1.
Z35905 Genomic DNA. Translation: CAA84978.1.
X76078 Genomic DNA. No translation available.
X17441 Genomic DNA. Translation: CAA35491.1.
BK006936 Genomic DNA. Translation: DAA07156.1.
PIRiS45894.
RefSeqiNP_009592.1. NM_001178384.1.

3D structure databases

ProteinModelPortaliP35206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32737. 452 interactions.
DIPiDIP-8095N.
IntActiP35206. 37 interactions.
MINTiMINT-1354206.
STRINGi4932.YBR036C.

Protein family/group databases

TCDBi2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiP35206.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR036C; YBR036C; YBR036C.
GeneIDi852324.
KEGGisce:YBR036C.

Organism-specific databases

SGDiS000000240. CSG2.

Phylogenomic databases

eggNOGiNOG70202.
HOGENOMiHOG000172600.
InParanoidiP35206.
OMAiRWEVIGY.
OrthoDBiEOG7JHMFW.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-695.
YEAST:MONOMER3O-695.

Miscellaneous databases

NextBioi971026.

Gene expression databases

GenevestigatoriP35206.

Family and domain databases

InterProiIPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae."
    Beeler T.J., Gable K., Zhao C., Dunn T.
    J. Biol. Chem. 269:7279-7284(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast."
    Takita Y., Ohya Y., Anraku Y.
    Mol. Gen. Genet. 246:269-281(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-410.
    Strain: ATCC 204508 / S288c.
  6. "Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene."
    Schulze M., Roedel G.
    Mol. Gen. Genet. 216:37-43(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
  7. "Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p."
    Uemura S., Kihara A., Inokuchi J., Igarashi Y.
    J. Biol. Chem. 278:45049-45055(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiCSG2_YEAST
AccessioniPrimary (citable) accession number: P35206
Secondary accession number(s): D6VQ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 5, 2010
Last modified: January 7, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.