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P35206 (CSG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
Gene names
Name:CSG2
Synonyms:CLS2
Ordered Locus Names:YBR036C
ORF Names:YBR0404
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for calcium regulation. May regulate calcium accumulation by a non-vacuole organelle. Also regulates the activity of CSH1 and SUR1 during mannosyl phosphorylinositol ceramide synthesis. Ref.7

Subunit structure

Heterodimer of CSH1 and CSG2, and SUR1 and CSG2. Ref.7

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.2.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 410393Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
PRO_0000021012

Regions

Topological domain18 – 5033Lumenal Potential
Transmembrane51 – 7121Helical; Potential
Topological domain72 – 14170Cytoplasmic Potential
Transmembrane142 – 16120Helical; Potential
Topological domain162 – 1676Lumenal Potential
Transmembrane168 – 18720Helical; Potential
Topological domain188 – 19710Cytoplasmic Potential
Transmembrane198 – 21720Helical; Potential
Topological domain218 – 24528Lumenal Potential
Transmembrane246 – 26520Helical; Potential
Topological domain266 – 28520Cytoplasmic Potential
Transmembrane286 – 30520Helical; Potential
Topological domain306 – 32419Lumenal Potential
Transmembrane325 – 34420Helical; Potential
Topological domain345 – 35511Cytoplasmic Potential
Transmembrane356 – 37419Helical; Potential
Topological domain375 – 38511Lumenal Potential
Transmembrane386 – 40419Helical; Potential
Topological domain405 – 4106Cytoplasmic Potential

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation491N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict681L → W in AAA34533. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P35206 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 15780FEBA54E4FD2

FASTA41045,442
        10         20         30         40         50         60 
MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS SSLTFYLTFM 

        70         80         90        100        110        120 
YFASWLLLVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG SVTNEDVDTF SHVLDDPQPR 

       130        140        150        160        170        180 
IPAQQQKQKI ISVATFKYVA KLTVLALIMI VADLTYNMAL SLSPAFDVAL MQNTAIFEIV 

       190        200        210        220        230        240 
TLLYGVCGIS RKNYVFRNFL IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL 

       250        260        270        280        290        300 
FDRLKGALIC GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL 

       310        320        330        340        350        360 
LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK APAEYLTTCN 

       370        380        390        400        410 
LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS VTLGEGKYHH 

« Hide

References

« Hide 'large scale' references
[1]"A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae."
Beeler T.J., Gable K., Zhao C., Dunn T.
J. Biol. Chem. 269:7279-7284(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast."
Takita Y., Ohya Y., Anraku Y.
Mol. Gen. Genet. 246:269-281(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
Smits P.H.M., de Haan M., Maat C., Grivell L.A.
Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-410.
Strain: ATCC 204508 / S288c.
[6]"Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene."
Schulze M., Roedel G.
Mol. Gen. Genet. 216:37-43(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
[7]"Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p."
Uemura S., Kihara A., Inokuchi J., Igarashi Y.
J. Biol. Chem. 278:45049-45055(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L24113 mRNA. Translation: AAA34533.1.
D28120 Genomic DNA. Translation: BAA05666.1.
Z35905 Genomic DNA. Translation: CAA84978.1.
X76078 Genomic DNA. No translation available.
X17441 Genomic DNA. Translation: CAA35491.1.
BK006936 Genomic DNA. Translation: DAA07156.1.
PIRS45894.
RefSeqNP_009592.1. NM_001178384.1.

3D structure databases

ProteinModelPortalP35206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32737. 451 interactions.
DIPDIP-8095N.
IntActP35206. 37 interactions.
MINTMINT-1354206.
STRING4932.YBR036C.

Protein family/group databases

TCDB2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbP35206.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR036C; YBR036C; YBR036C.
GeneID852324.
KEGGsce:YBR036C.

Organism-specific databases

SGDS000000240. CSG2.

Phylogenomic databases

eggNOGNOG70202.
HOGENOMHOG000172600.
OMARWEVIGY.
OrthoDBEOG7JHMFW.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER3O-695.
YEAST:MONOMER3O-695.

Gene expression databases

GenevestigatorP35206.

Family and domain databases

InterProIPR018247. EF_Hand_1_Ca_BS.
[Graphical view]
ProtoNetSearch...

Other

NextBio971026.

Entry information

Entry nameCSG2_YEAST
AccessionPrimary (citable) accession number: P35206
Secondary accession number(s): D6VQ36
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD