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P35206

- CSG2_YEAST

UniProt

P35206 - CSG2_YEAST

Protein

Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2

Gene

CSG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Required for calcium regulation. May regulate calcium accumulation by a non-vacuole organelle. Also regulates the activity of CSH1 and SUR1 during mannosyl phosphorylinositol ceramide synthesis.1 Publication

    GO - Molecular functioni

    1. enzyme regulator activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cellular calcium ion homeostasis Source: SGD
    2. glycosphingolipid biosynthetic process Source: SGD
    3. regulation of catalytic activity Source: GOC

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-695.
    YEAST:MONOMER3O-695.

    Protein family/group databases

    TCDBi2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2
    Gene namesi
    Name:CSG2
    Synonyms:CLS2
    Ordered Locus Names:YBR036C
    ORF Names:YBR0404
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    SGDiS000000240. CSG2.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 410393Mannosyl phosphorylinositol ceramide synthase regulatory protein CSG2PRO_0000021012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP35206.

    Expressioni

    Gene expression databases

    GenevestigatoriP35206.

    Interactioni

    Subunit structurei

    Heterodimer of CSH1 and CSG2, and SUR1 and CSG2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CSH1P382872EBI-2051140,EBI-20861
    SHR3Q027743EBI-2051140,EBI-17099

    Protein-protein interaction databases

    BioGridi32737. 452 interactions.
    DIPiDIP-8095N.
    IntActiP35206. 37 interactions.
    MINTiMINT-1354206.
    STRINGi4932.YBR036C.

    Structurei

    3D structure databases

    ProteinModelPortaliP35206.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini18 – 5033LumenalSequence AnalysisAdd
    BLAST
    Topological domaini72 – 14170CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini162 – 1676LumenalSequence Analysis
    Topological domaini188 – 19710CytoplasmicSequence Analysis
    Topological domaini218 – 24528LumenalSequence AnalysisAdd
    BLAST
    Topological domaini266 – 28520CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini306 – 32419LumenalSequence AnalysisAdd
    BLAST
    Topological domaini345 – 35511CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini375 – 38511LumenalSequence AnalysisAdd
    BLAST
    Topological domaini405 – 4106CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei51 – 7121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei142 – 16120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei168 – 18720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei198 – 21720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei246 – 26520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei286 – 30520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei325 – 34420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei356 – 37419HelicalSequence AnalysisAdd
    BLAST
    Transmembranei386 – 40419HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG70202.
    HOGENOMiHOG000172600.
    OMAiRWEVIGY.
    OrthoDBiEOG7JHMFW.

    Family and domain databases

    InterProiIPR018247. EF_Hand_1_Ca_BS.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35206-1 [UniParc]FASTAAdd to Basket

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    MSTTLLWFSS VIGYVIQTKC LSNIQSKKEI SVGPNGTIAT PETNGDNGNS    50
    SSLTFYLTFM YFASWLLLVP ASRLWEKMRP MFVSDSDSNR NSQFDNNNSG 100
    SVTNEDVDTF SHVLDDPQPR IPAQQQKQKI ISVATFKYVA KLTVLALIMI 150
    VADLTYNMAL SLSPAFDVAL MQNTAIFEIV TLLYGVCGIS RKNYVFRNFL 200
    IMMNAVIGIL IISYTKATCD MLAGKLSVNP NTGELSDPFL FDRLKGALIC 250
    GLGALIMGPF AVLWNRWFCS NISKNENSAV VLVKQSTHMA LIGIIGMVIL 300
    LPFIPKFPSR ESVESISLFY NDKSFWFSLL GSIIFGSLPS LISILELNRK 350
    APAEYLTTCN LGAIIFMGLA EWVCEPTQTT IVRWEVIGYI MLTVSLLVLS 400
    VTLGEGKYHH 410
    Length:410
    Mass (Da):45,442
    Last modified:October 5, 2010 - v2
    Checksum:i15780FEBA54E4FD2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681L → W in AAA34533. (PubMed:8125941)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24113 mRNA. Translation: AAA34533.1.
    D28120 Genomic DNA. Translation: BAA05666.1.
    Z35905 Genomic DNA. Translation: CAA84978.1.
    X76078 Genomic DNA. No translation available.
    X17441 Genomic DNA. Translation: CAA35491.1.
    BK006936 Genomic DNA. Translation: DAA07156.1.
    PIRiS45894.
    RefSeqiNP_009592.1. NM_001178384.1.

    Genome annotation databases

    EnsemblFungiiYBR036C; YBR036C; YBR036C.
    GeneIDi852324.
    KEGGisce:YBR036C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L24113 mRNA. Translation: AAA34533.1 .
    D28120 Genomic DNA. Translation: BAA05666.1 .
    Z35905 Genomic DNA. Translation: CAA84978.1 .
    X76078 Genomic DNA. No translation available.
    X17441 Genomic DNA. Translation: CAA35491.1 .
    BK006936 Genomic DNA. Translation: DAA07156.1 .
    PIRi S45894.
    RefSeqi NP_009592.1. NM_001178384.1.

    3D structure databases

    ProteinModelPortali P35206.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32737. 452 interactions.
    DIPi DIP-8095N.
    IntActi P35206. 37 interactions.
    MINTi MINT-1354206.
    STRINGi 4932.YBR036C.

    Protein family/group databases

    TCDBi 2.A.7.27.1. the drug/metabolite transporter (dmt) superfamily.

    Proteomic databases

    PaxDbi P35206.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR036C ; YBR036C ; YBR036C .
    GeneIDi 852324.
    KEGGi sce:YBR036C.

    Organism-specific databases

    SGDi S000000240. CSG2.

    Phylogenomic databases

    eggNOGi NOG70202.
    HOGENOMi HOG000172600.
    OMAi RWEVIGY.
    OrthoDBi EOG7JHMFW.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER3O-695.
    YEAST:MONOMER3O-695.

    Miscellaneous databases

    NextBioi 971026.

    Gene expression databases

    Genevestigatori P35206.

    Family and domain databases

    InterProi IPR018247. EF_Hand_1_Ca_BS.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel protein, CSG2p, is required for Ca2+ regulation in Saccharomyces cerevisiae."
      Beeler T.J., Gable K., Zhao C., Dunn T.
      J. Biol. Chem. 269:7279-7284(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast."
      Takita Y., Ohya Y., Anraku Y.
      Mol. Gen. Genet. 246:269-281(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
      Smits P.H.M., de Haan M., Maat C., Grivell L.A.
      Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-410.
      Strain: ATCC 204508 / S288c.
    6. "Accumulation of the cytochrome c oxidase subunits I and II in yeast requires a mitochondrial membrane-associated protein, encoded by the nuclear SCO1 gene."
      Schulze M., Roedel G.
      Mol. Gen. Genet. 216:37-43(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
    7. "Csg1p and newly identified Csh1p function in mannosylinositol phosphorylceramide synthesis by interacting with Csg2p."
      Uemura S., Kihara A., Inokuchi J., Igarashi Y.
      J. Biol. Chem. 278:45049-45055(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiCSG2_YEAST
    AccessioniPrimary (citable) accession number: P35206
    Secondary accession number(s): D6VQ36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3