Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P35203 (CBF3C_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere DNA-binding protein complex CBF3 subunit C
Alternative name(s):
Chromosome transmission fidelity protein 13
Kinetochore protein CTF13
Gene names
Name:CTF13
Synonyms:CBF3C
Ordered Locus Names:YMR094W
ORF Names:YM6543.01, YM9582.19
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly.

Subunit structure

Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF3C interacts with CBF3D and SGT1. Ref.5

Subcellular location

Nucleus. Chromosomecentromere.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 478477Centromere DNA-binding protein complex CBF3 subunit C
PRO_0000089370

Regions

Compositional bias198 – 22225Asp/Glu-rich (acidic)
Compositional bias216 – 22914Ser-rich

Sequences

Sequence LengthMass (Da)Tools
P35203 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 77B1C012FD74E56F

FASTA47856,336
        10         20         30         40         50         60 
MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK PSYKRSKRSK 

        70         80         90        100        110        120 
KLLRYMYPVF ATYLNIFEYS PQLIEKWLEY AFWLRYDCLV LDCFKVNHLY DGTLIDALEW 

       130        140        150        160        170        180 
TYLDNELRLA YFNKASMLEV WYTFKEYKKW VIDSVAFDEL DLLNVSNIQF NIDNLTPQLV 

       190        200        210        220        230        240 
DKCLSILEQK DLFATIGEVQ FGQDEEVGEE KDVDVSGANS DENSSPSSTI KNKKRSASKR 

       250        260        270        280        290        300 
SHSDNGNVGA THNQLTSISV IRTIRSMESM KSLRKITVRG EKLYELLINF HGFRDNPGKT 

       310        320        330        340        350        360 
ISYIVKRRIN EIRLSRMNQI SRTGLADFTR WDNLQKLVLS RVAYIDLNSI VFPKNFKSLT 

       370        380        390        400        410        420 
MKRVSKIKWW NIEENILKEL KVDKRTFKSL YIKEDDSKFT KFFNLRHTRI KELDKSEINQ 

       430        440        450        460        470 
ITYLRCQAIV WLSFRTLNHI KLQNVSEVFN NIIVPRALFD SKRVEIYRCE KISQVLVI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of essential components of the S. cerevisiae kinetochore."
Doheny K.F., Sorger P.K., Hyman A.A., Tugendreich S., Spencer F., Hieter P.
Cell 73:761-774(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3."
Lechner J.
EMBO J. 13:5203-5211(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-8; 10-17 AND 342-349.
[5]"Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function."
Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B.
Mol. Biol. Cell 15:3366-3378(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSEMBLY OF THE CBF3 COMPLEX, INTERACTION WITH CBF3D AND SGT1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10063 Genomic DNA. Translation: AAA34536.1.
Z49259 Genomic DNA. Translation: CAA89241.1.
Z49807 Genomic DNA. Translation: CAA89895.1.
BK006946 Genomic DNA. Translation: DAA09991.1.
PIRA40747.
RefSeqNP_013812.1. NM_001182594.1.

3D structure databases

ProteinModelPortalP35203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35269. 39 interactions.
DIPDIP-1277N.
IntActP35203. 7 interactions.
MINTMINT-392923.
STRING4932.YMR094W.

Proteomic databases

MaxQBP35203.
PaxDbP35203.
PeptideAtlasP35203.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR094W; YMR094W; YMR094W.
GeneID855119.
KEGGsce:YMR094W.

Organism-specific databases

SGDS000004700. CTF13.

Phylogenomic databases

eggNOGNOG42730.
HOGENOMHOG000111346.
KOK11576.
OMAGARDNPG.
OrthoDBEOG73BVQJ.

Enzyme and pathway databases

BioCycYEAST:G3O-32794-MONOMER.

Gene expression databases

GenevestigatorP35203.

Family and domain databases

ProtoNetSearch...

Other

NextBio978470.

Entry information

Entry nameCBF3C_YEAST
AccessionPrimary (citable) accession number: P35203
Secondary accession number(s): D6VZR7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD