Reviewed, UniProtKB/Swiss-Prot P35203 (CBF3C_YEAST)

Names and origin

Protein names

Recommended name:
    Centromere DNA-binding protein complex CBF3 subunit C
Alternative name(s):
    Chromosome transmission fidelity protein 13
    Kinetochore protein CTF13

Gene names

Name:	CTF13
Synonyms:	CBF3C
Ordered Locus Names:	YMR094W
ORF Names:	YM9582.19, YM6543.01

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly.
Subunit structure	Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF3C interacts with CBF3D and SGT1. Ref.4
Subcellular location	Nucleus. Centromere.

Ontologies

Keywords
Cellular component	Centromere Chromosomal protein Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	kinetochore assembly Inferred from direct assay. Source: SGD septin ring assembly Inferred from mutant phenotype. Source: SGD
Cellular component	CBF3 complex Inferred from direct assay. Source: SGD condensed nuclear chromosome kinetochore Traceable author statement. Source: SGD
Molecular function	DNA bending activity Inferred from direct assay. Source: SGD centromeric DNA binding Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAM1	P53267	1	EBI-4085,EBI-23268
SKP1	P52286	1	EBI-4085,EBI-4090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 478	477	Centromere DNA-binding protein complex CBF3 subunit C		PRO_0000089370
Regions
	Compositional bias	198 – 222	25	Asp/Glu-rich (acidic)
	Compositional bias	216 – 229	14	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	P35203-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 77B1C012FD74E56F	FASTA	478	56,336
	10 20 30 40 50 60 MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK PSYKRSKRSK 70 80 90 100 110 120 KLLRYMYPVF ATYLNIFEYS PQLIEKWLEY AFWLRYDCLV LDCFKVNHLY DGTLIDALEW 130 140 150 160 170 180 TYLDNELRLA YFNKASMLEV WYTFKEYKKW VIDSVAFDEL DLLNVSNIQF NIDNLTPQLV 190 200 210 220 230 240 DKCLSILEQK DLFATIGEVQ FGQDEEVGEE KDVDVSGANS DENSSPSSTI KNKKRSASKR 250 260 270 280 290 300 SHSDNGNVGA THNQLTSISV IRTIRSMESM KSLRKITVRG EKLYELLINF HGFRDNPGKT 310 320 330 340 350 360 ISYIVKRRIN EIRLSRMNQI SRTGLADFTR WDNLQKLVLS RVAYIDLNSI VFPKNFKSLT 370 380 390 400 410 420 MKRVSKIKWW NIEENILKEL KVDKRTFKSL YIKEDDSKFT KFFNLRHTRI KELDKSEINQ 430 440 450 460 470 ITYLRCQAIV WLSFRTLNHI KLQNVSEVFN NIIVPRALFD SKRVEIYRCE KISQVLVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of essential components of the S. cerevisiae kinetochore." Doheny K.F., Sorger P.K., Hyman A.A., Tugendreich S., Spencer F., Hieter P. Cell 73:761-774(1993) [PubMed: 8500169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3." Lechner J. EMBO J. 13:5203-5211(1994) [PubMed: 7957085] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8; 10-17 AND 342-349.
[4]	"Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function." Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B. Mol. Biol. Cell 15:3366-3378(2004) [PubMed: 15090617] [Abstract] Cited for: ASSEMBLY OF THE CBF3 COMPLEX, INTERACTION WITH CBF3D AND SGT1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10063 Genomic DNA. Translation: AAA34536.1. Z49259 Genomic DNA. Translation: CAA89241.1. Z49807 Genomic DNA. Translation: CAA89895.1.
PIR	A40747.
RefSeq	NP_013812.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1277N.
IntAct	P35203. 6 interactions.
Proteomic databases
PeptideAtlas	P35203.
PRIDE	P35203.
Genome annotation databases
Ensembl	YMR094W. Saccharomyces cerevisiae. [Contig view]
GeneID	855119.
GenomeReviews	Gene locus YMR094W in contig Z71257_GR.
KEGG	sce:YMR094W.
NMPDR	fig|4932.3.peg.4859.
Organism-specific databases
CYGD	YMR094w.
SGD	S000004700. CTF13.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P35203.
OMA	P35203. GARDNPG.
Gene expression databases
GermOnline	YMR094W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	978470.

Entry information

Entry name

CBF3C_YEAST

Accession

Primary (citable) accession number: P35203

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)