Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35203

- CBF3C_YEAST

UniProt

P35203 - CBF3C_YEAST

Protein

Centromere DNA-binding protein complex CBF3 subunit C

Gene

CTF13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Acts as central component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly.

    GO - Molecular functioni

    1. centromeric DNA binding Source: SGD
    2. DNA binding, bending Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. kinetochore assembly Source: SGD
    2. septin ring assembly Source: SGD

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32794-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere DNA-binding protein complex CBF3 subunit C
    Alternative name(s):
    Chromosome transmission fidelity protein 13
    Kinetochore protein CTF13
    Gene namesi
    Name:CTF13
    Synonyms:CBF3C
    Ordered Locus Names:YMR094W
    ORF Names:YM6543.01, YM9582.19
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004700. CTF13.

    Subcellular locationi

    GO - Cellular componenti

    1. CBF3 complex Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 478477Centromere DNA-binding protein complex CBF3 subunit CPRO_0000089370Add
    BLAST

    Proteomic databases

    MaxQBiP35203.
    PaxDbiP35203.
    PeptideAtlasiP35203.

    Expressioni

    Gene expression databases

    GenevestigatoriP35203.

    Interactioni

    Subunit structurei

    Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF3C interacts with CBF3D and SGT1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CBF2P325043EBI-4085,EBI-4069
    CEP3P409692EBI-4085,EBI-4077
    DAM1P532672EBI-4085,EBI-23268
    SGT1Q084463EBI-4085,EBI-17070
    SKP1P522865EBI-4085,EBI-4090

    Protein-protein interaction databases

    BioGridi35269. 39 interactions.
    DIPiDIP-1277N.
    IntActiP35203. 7 interactions.
    MINTiMINT-392923.
    STRINGi4932.YMR094W.

    Structurei

    3D structure databases

    ProteinModelPortaliP35203.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi198 – 22225Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi216 – 22914Ser-richAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG42730.
    HOGENOMiHOG000111346.
    KOiK11576.
    OMAiGARDNPG.
    OrthoDBiEOG73BVQJ.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35203-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK    50
    PSYKRSKRSK KLLRYMYPVF ATYLNIFEYS PQLIEKWLEY AFWLRYDCLV 100
    LDCFKVNHLY DGTLIDALEW TYLDNELRLA YFNKASMLEV WYTFKEYKKW 150
    VIDSVAFDEL DLLNVSNIQF NIDNLTPQLV DKCLSILEQK DLFATIGEVQ 200
    FGQDEEVGEE KDVDVSGANS DENSSPSSTI KNKKRSASKR SHSDNGNVGA 250
    THNQLTSISV IRTIRSMESM KSLRKITVRG EKLYELLINF HGFRDNPGKT 300
    ISYIVKRRIN EIRLSRMNQI SRTGLADFTR WDNLQKLVLS RVAYIDLNSI 350
    VFPKNFKSLT MKRVSKIKWW NIEENILKEL KVDKRTFKSL YIKEDDSKFT 400
    KFFNLRHTRI KELDKSEINQ ITYLRCQAIV WLSFRTLNHI KLQNVSEVFN 450
    NIIVPRALFD SKRVEIYRCE KISQVLVI 478
    Length:478
    Mass (Da):56,336
    Last modified:January 23, 2007 - v3
    Checksum:i77B1C012FD74E56F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10063 Genomic DNA. Translation: AAA34536.1.
    Z49259 Genomic DNA. Translation: CAA89241.1.
    Z49807 Genomic DNA. Translation: CAA89895.1.
    BK006946 Genomic DNA. Translation: DAA09991.1.
    PIRiA40747.
    RefSeqiNP_013812.1. NM_001182594.1.

    Genome annotation databases

    EnsemblFungiiYMR094W; YMR094W; YMR094W.
    GeneIDi855119.
    KEGGisce:YMR094W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10063 Genomic DNA. Translation: AAA34536.1 .
    Z49259 Genomic DNA. Translation: CAA89241.1 .
    Z49807 Genomic DNA. Translation: CAA89895.1 .
    BK006946 Genomic DNA. Translation: DAA09991.1 .
    PIRi A40747.
    RefSeqi NP_013812.1. NM_001182594.1.

    3D structure databases

    ProteinModelPortali P35203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35269. 39 interactions.
    DIPi DIP-1277N.
    IntActi P35203. 7 interactions.
    MINTi MINT-392923.
    STRINGi 4932.YMR094W.

    Proteomic databases

    MaxQBi P35203.
    PaxDbi P35203.
    PeptideAtlasi P35203.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR094W ; YMR094W ; YMR094W .
    GeneIDi 855119.
    KEGGi sce:YMR094W.

    Organism-specific databases

    SGDi S000004700. CTF13.

    Phylogenomic databases

    eggNOGi NOG42730.
    HOGENOMi HOG000111346.
    KOi K11576.
    OMAi GARDNPG.
    OrthoDBi EOG73BVQJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32794-MONOMER.

    Miscellaneous databases

    NextBioi 978470.

    Gene expression databases

    Genevestigatori P35203.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Identification of essential components of the S. cerevisiae kinetochore."
      Doheny K.F., Sorger P.K., Hyman A.A., Tugendreich S., Spencer F., Hieter P.
      Cell 73:761-774(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3."
      Lechner J.
      EMBO J. 13:5203-5211(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-8; 10-17 AND 342-349.
    5. "Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function."
      Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B.
      Mol. Biol. Cell 15:3366-3378(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSEMBLY OF THE CBF3 COMPLEX, INTERACTION WITH CBF3D AND SGT1.

    Entry informationi

    Entry nameiCBF3C_YEAST
    AccessioniPrimary (citable) accession number: P35203
    Secondary accession number(s): D6VZR7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3