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Protein

Thiamine pyrophosphokinase

Gene

THI80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase (THI80)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: SGD

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YOR143C-MONOMER.
YEAST:YOR143C-MONOMER.
ReactomeiR-SCE-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase (EC:2.7.6.2)
Short name:
TPK
Short name:
Thiamine kinase
Gene namesi
Name:THI80
Ordered Locus Names:YOR143C
ORF Names:YOR3373C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR143C.
SGDiS000005669. THI80.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000725132 – 319Thiamine pyrophosphokinaseAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP35202.
PRIDEiP35202.

Expressioni

Inductioni

Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34540. 12 interactors.
DIPiDIP-5324N.
IntActiP35202. 5 interactors.
MINTiMINT-520646.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi10 – 12Combined sources3
Beta strandi23 – 27Combined sources5
Helixi28 – 30Combined sources3
Beta strandi37 – 43Combined sources7
Helixi52 – 61Combined sources10
Beta strandi63 – 68Combined sources6
Helixi71 – 78Combined sources8
Helixi83 – 86Combined sources4
Beta strandi92 – 96Combined sources5
Helixi103 – 111Combined sources9
Beta strandi115 – 118Combined sources4
Beta strandi122 – 124Combined sources3
Helixi126 – 139Combined sources14
Helixi141 – 147Combined sources7
Turni153 – 155Combined sources3
Helixi156 – 158Combined sources3
Turni161 – 163Combined sources3
Helixi164 – 173Combined sources10
Helixi178 – 180Combined sources3
Beta strandi184 – 195Combined sources12
Helixi196 – 212Combined sources17
Beta strandi216 – 221Combined sources6
Beta strandi223 – 230Combined sources8
Beta strandi235 – 238Combined sources4
Helixi241 – 247Combined sources7
Beta strandi248 – 254Combined sources7
Beta strandi260 – 278Combined sources19
Turni279 – 282Combined sources4
Beta strandi285 – 288Combined sources4
Beta strandi292 – 302Combined sources11
Beta strandi304 – 310Combined sources7
Helixi312 – 315Combined sources4
Helixi316 – 318Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG0X-ray1.80A/B1-319[»]
ProteinModelPortaliP35202.
SMRiP35202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35202.

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016016.
HOGENOMiHOG000248374.
InParanoidiP35202.
KOiK00949.
OMAiCIGNCGL.
OrthoDBiEOG092C5171.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 2 hits.
InterProiIPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35202-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI
60 70 80 90 100
PRPLFYKIWK LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS
110 120 130 140 150
LSEKVYKYYR KNKVTIIKQT TQYSTDFTKC VNLISLHFNS PEFRSLISNK
160 170 180 190 200
DNLQSNHGIE LEKGIHTLYN TMTESLVFSK VTPISLLALG GIGGRFDQTV
210 220 230 240 250
HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD PQFRNTCIGN
260 270 280 290 300
CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT
310
KDDIILNVEI FVDKLIDFL
Length:319
Mass (Da):36,616
Last modified:February 1, 1994 - v1
Checksum:iB82609E195D4D53F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14417 Genomic DNA. Translation: BAA03312.1.
X94335 Genomic DNA. Translation: CAA64061.1.
Z75051 Genomic DNA. Translation: CAA99346.1.
BK006948 Genomic DNA. Translation: DAA10917.1.
PIRiA47499.
RefSeqiNP_014786.1. NM_001183562.1.

Genome annotation databases

EnsemblFungiiYOR143C; YOR143C; YOR143C.
GeneIDi854314.
KEGGisce:YOR143C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14417 Genomic DNA. Translation: BAA03312.1.
X94335 Genomic DNA. Translation: CAA64061.1.
Z75051 Genomic DNA. Translation: CAA99346.1.
BK006948 Genomic DNA. Translation: DAA10917.1.
PIRiA47499.
RefSeqiNP_014786.1. NM_001183562.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IG0X-ray1.80A/B1-319[»]
ProteinModelPortaliP35202.
SMRiP35202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34540. 12 interactors.
DIPiDIP-5324N.
IntActiP35202. 5 interactors.
MINTiMINT-520646.

Proteomic databases

MaxQBiP35202.
PRIDEiP35202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR143C; YOR143C; YOR143C.
GeneIDi854314.
KEGGisce:YOR143C.

Organism-specific databases

EuPathDBiFungiDB:YOR143C.
SGDiS000005669. THI80.

Phylogenomic databases

GeneTreeiENSGT00390000016016.
HOGENOMiHOG000248374.
InParanoidiP35202.
KOiK00949.
OMAiCIGNCGL.
OrthoDBiEOG092C5171.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00597.
BioCyciMetaCyc:YOR143C-MONOMER.
YEAST:YOR143C-MONOMER.
ReactomeiR-SCE-196819. Vitamin B1 (thiamin) metabolism.

Miscellaneous databases

EvolutionaryTraceiP35202.
PROiP35202.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 2 hits.
InterProiIPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI80_YEAST
AccessioniPrimary (citable) accession number: P35202
Secondary accession number(s): D6W2K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.