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P35202

- THI80_YEAST

UniProt

P35202 - THI80_YEAST

Protein

Thiamine pyrophosphokinase

Gene

THI80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

    Catalytic activityi

    ATP + thiamine = AMP + thiamine diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: UniProtKB-KW
    3. thiamine diphosphokinase activity Source: SGD

    GO - Biological processi

    1. thiamine diphosphate biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YOR143C-MONOMER.
    UniPathwayiUPA00060; UER00597.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thiamine pyrophosphokinase (EC:2.7.6.2)
    Short name:
    TPK
    Short name:
    Thiamine kinase
    Gene namesi
    Name:THI80
    Ordered Locus Names:YOR143C
    ORF Names:YOR3373C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR143c.
    SGDiS000005669. THI80.

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 319318Thiamine pyrophosphokinasePRO_0000072513Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP35202.
    PaxDbiP35202.

    Expressioni

    Inductioni

    Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

    Gene expression databases

    GenevestigatoriP35202.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi34540. 10 interactions.
    DIPiDIP-5324N.
    IntActiP35202. 5 interactions.
    MINTiMINT-520646.
    STRINGi4932.YOR143C.

    Structurei

    Secondary structure

    1
    319
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 74
    Beta strandi10 – 123
    Beta strandi23 – 275
    Helixi28 – 303
    Beta strandi37 – 437
    Helixi52 – 6110
    Beta strandi63 – 686
    Helixi71 – 788
    Helixi83 – 864
    Beta strandi92 – 965
    Helixi103 – 1119
    Beta strandi115 – 1184
    Beta strandi122 – 1243
    Helixi126 – 13914
    Helixi141 – 1477
    Turni153 – 1553
    Helixi156 – 1583
    Turni161 – 1633
    Helixi164 – 17310
    Helixi178 – 1803
    Beta strandi184 – 19512
    Helixi196 – 21217
    Beta strandi216 – 2216
    Beta strandi223 – 2308
    Beta strandi235 – 2384
    Helixi241 – 2477
    Beta strandi248 – 2547
    Beta strandi260 – 27819
    Turni279 – 2824
    Beta strandi285 – 2884
    Beta strandi292 – 30211
    Beta strandi304 – 3107
    Helixi312 – 3154
    Helixi316 – 3183

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IG0X-ray1.80A/B1-319[»]
    ProteinModelPortaliP35202.
    SMRiP35202. Positions 1-319.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35202.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thiamine pyrophosphokinase family.Curated

    Phylogenomic databases

    eggNOGiCOG1564.
    GeneTreeiENSGT00390000016016.
    HOGENOMiHOG000248374.
    KOiK00949.
    OMAiCIGNCGL.
    OrthoDBiEOG7ZKSPD.

    Family and domain databases

    Gene3Di2.60.120.320. 1 hit.
    3.40.50.10240. 2 hits.
    InterProiIPR016966. Thiamin_pyrophosphokinase_euk.
    IPR007373. Thiamin_PyroPKinase_B1-bd.
    IPR007371. TPK_catalytic.
    [Graphical view]
    PfamiPF04265. TPK_B1_binding. 1 hit.
    PF04263. TPK_catalytic. 1 hit.
    [Graphical view]
    PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
    SMARTiSM00983. TPK_B1_binding. 1 hit.
    [Graphical view]
    SUPFAMiSSF63862. SSF63862. 1 hit.
    SSF63999. SSF63999. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35202-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI    50
    PRPLFYKIWK LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS 100
    LSEKVYKYYR KNKVTIIKQT TQYSTDFTKC VNLISLHFNS PEFRSLISNK 150
    DNLQSNHGIE LEKGIHTLYN TMTESLVFSK VTPISLLALG GIGGRFDQTV 200
    HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD PQFRNTCIGN 250
    CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT 300
    KDDIILNVEI FVDKLIDFL 319
    Length:319
    Mass (Da):36,616
    Last modified:February 1, 1994 - v1
    Checksum:iB82609E195D4D53F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14417 Genomic DNA. Translation: BAA03312.1.
    X94335 Genomic DNA. Translation: CAA64061.1.
    Z75051 Genomic DNA. Translation: CAA99346.1.
    BK006948 Genomic DNA. Translation: DAA10917.1.
    PIRiA47499.
    RefSeqiNP_014786.1. NM_001183562.1.

    Genome annotation databases

    EnsemblFungiiYOR143C; YOR143C; YOR143C.
    GeneIDi854314.
    KEGGisce:YOR143C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14417 Genomic DNA. Translation: BAA03312.1 .
    X94335 Genomic DNA. Translation: CAA64061.1 .
    Z75051 Genomic DNA. Translation: CAA99346.1 .
    BK006948 Genomic DNA. Translation: DAA10917.1 .
    PIRi A47499.
    RefSeqi NP_014786.1. NM_001183562.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IG0 X-ray 1.80 A/B 1-319 [» ]
    ProteinModelPortali P35202.
    SMRi P35202. Positions 1-319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34540. 10 interactions.
    DIPi DIP-5324N.
    IntActi P35202. 5 interactions.
    MINTi MINT-520646.
    STRINGi 4932.YOR143C.

    Chemistry

    DrugBanki DB00152. Thiamine.

    Proteomic databases

    MaxQBi P35202.
    PaxDbi P35202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR143C ; YOR143C ; YOR143C .
    GeneIDi 854314.
    KEGGi sce:YOR143C.

    Organism-specific databases

    CYGDi YOR143c.
    SGDi S000005669. THI80.

    Phylogenomic databases

    eggNOGi COG1564.
    GeneTreei ENSGT00390000016016.
    HOGENOMi HOG000248374.
    KOi K00949.
    OMAi CIGNCGL.
    OrthoDBi EOG7ZKSPD.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00597 .
    BioCyci YEAST:YOR143C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P35202.
    NextBioi 976339.
    PROi P35202.

    Gene expression databases

    Genevestigatori P35202.

    Family and domain databases

    Gene3Di 2.60.120.320. 1 hit.
    3.40.50.10240. 2 hits.
    InterProi IPR016966. Thiamin_pyrophosphokinase_euk.
    IPR007373. Thiamin_PyroPKinase_B1-bd.
    IPR007371. TPK_catalytic.
    [Graphical view ]
    Pfami PF04265. TPK_B1_binding. 1 hit.
    PF04263. TPK_catalytic. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
    SMARTi SM00983. TPK_B1_binding. 1 hit.
    [Graphical view ]
    SUPFAMi SSF63862. SSF63862. 1 hit.
    SSF63999. SSF63999. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae."
      Nosaka K., Kaneko Y., Nishimura H., Iwashima A.
      J. Biol. Chem. 268:17440-17447(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    7. "The crystal structure of yeast thiamin pyrophosphokinase."
      Baker L.-J., Dorocke J.A., Harris R.A., Timm D.E.
      Structure 9:539-546(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

    Entry informationi

    Entry nameiTHI80_YEAST
    AccessioniPrimary (citable) accession number: P35202
    Secondary accession number(s): D6W2K1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3320 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3