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P35202

- THI80_YEAST

UniProt

P35202 - THI80_YEAST

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Protein
Thiamine pyrophosphokinase
Gene
THI80, YOR143C, YOR3373C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: UniProtKB-KW
  3. thiamine diphosphokinase activity Source: SGD

GO - Biological processi

  1. thiamine diphosphate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOR143C-MONOMER.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase (EC:2.7.6.2)
Short name:
TPK
Short name:
Thiamine kinase
Gene namesi
Name:THI80
Ordered Locus Names:YOR143C
ORF Names:YOR3373C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR143c.
SGDiS000005669. THI80.

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 319318Thiamine pyrophosphokinase
PRO_0000072513Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP35202.
PaxDbiP35202.

Expressioni

Inductioni

Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

Gene expression databases

GenevestigatoriP35202.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34540. 10 interactions.
DIPiDIP-5324N.
IntActiP35202. 5 interactions.
MINTiMINT-520646.
STRINGi4932.YOR143C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74
Beta strandi10 – 123
Beta strandi23 – 275
Helixi28 – 303
Beta strandi37 – 437
Helixi52 – 6110
Beta strandi63 – 686
Helixi71 – 788
Helixi83 – 864
Beta strandi92 – 965
Helixi103 – 1119
Beta strandi115 – 1184
Beta strandi122 – 1243
Helixi126 – 13914
Helixi141 – 1477
Turni153 – 1553
Helixi156 – 1583
Turni161 – 1633
Helixi164 – 17310
Helixi178 – 1803
Beta strandi184 – 19512
Helixi196 – 21217
Beta strandi216 – 2216
Beta strandi223 – 2308
Beta strandi235 – 2384
Helixi241 – 2477
Beta strandi248 – 2547
Beta strandi260 – 27819
Turni279 – 2824
Beta strandi285 – 2884
Beta strandi292 – 30211
Beta strandi304 – 3107
Helixi312 – 3154
Helixi316 – 3183

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IG0X-ray1.80A/B1-319[»]
ProteinModelPortaliP35202.
SMRiP35202. Positions 1-319.

Miscellaneous databases

EvolutionaryTraceiP35202.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1564.
GeneTreeiENSGT00390000016016.
HOGENOMiHOG000248374.
KOiK00949.
OMAiCIGNCGL.
OrthoDBiEOG7ZKSPD.

Family and domain databases

Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 2 hits.
InterProiIPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamiPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35202-1 [UniParc]FASTAAdd to Basket

« Hide

MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI    50
PRPLFYKIWK LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS 100
LSEKVYKYYR KNKVTIIKQT TQYSTDFTKC VNLISLHFNS PEFRSLISNK 150
DNLQSNHGIE LEKGIHTLYN TMTESLVFSK VTPISLLALG GIGGRFDQTV 200
HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD PQFRNTCIGN 250
CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT 300
KDDIILNVEI FVDKLIDFL 319
Length:319
Mass (Da):36,616
Last modified:February 1, 1994 - v1
Checksum:iB82609E195D4D53F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14417 Genomic DNA. Translation: BAA03312.1.
X94335 Genomic DNA. Translation: CAA64061.1.
Z75051 Genomic DNA. Translation: CAA99346.1.
BK006948 Genomic DNA. Translation: DAA10917.1.
PIRiA47499.
RefSeqiNP_014786.1. NM_001183562.1.

Genome annotation databases

EnsemblFungiiYOR143C; YOR143C; YOR143C.
GeneIDi854314.
KEGGisce:YOR143C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14417 Genomic DNA. Translation: BAA03312.1 .
X94335 Genomic DNA. Translation: CAA64061.1 .
Z75051 Genomic DNA. Translation: CAA99346.1 .
BK006948 Genomic DNA. Translation: DAA10917.1 .
PIRi A47499.
RefSeqi NP_014786.1. NM_001183562.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IG0 X-ray 1.80 A/B 1-319 [» ]
ProteinModelPortali P35202.
SMRi P35202. Positions 1-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34540. 10 interactions.
DIPi DIP-5324N.
IntActi P35202. 5 interactions.
MINTi MINT-520646.
STRINGi 4932.YOR143C.

Chemistry

DrugBanki DB00152. Thiamine.

Proteomic databases

MaxQBi P35202.
PaxDbi P35202.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR143C ; YOR143C ; YOR143C .
GeneIDi 854314.
KEGGi sce:YOR143C.

Organism-specific databases

CYGDi YOR143c.
SGDi S000005669. THI80.

Phylogenomic databases

eggNOGi COG1564.
GeneTreei ENSGT00390000016016.
HOGENOMi HOG000248374.
KOi K00949.
OMAi CIGNCGL.
OrthoDBi EOG7ZKSPD.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00597 .
BioCyci YEAST:YOR143C-MONOMER.

Miscellaneous databases

EvolutionaryTracei P35202.
NextBioi 976339.
PROi P35202.

Gene expression databases

Genevestigatori P35202.

Family and domain databases

Gene3Di 2.60.120.320. 1 hit.
3.40.50.10240. 2 hits.
InterProi IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view ]
Pfami PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view ]
PIRSFi PIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTi SM00983. TPK_B1_binding. 1 hit.
[Graphical view ]
SUPFAMi SSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae."
    Nosaka K., Kaneko Y., Nishimura H., Iwashima A.
    J. Biol. Chem. 268:17440-17447(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  7. "The crystal structure of yeast thiamin pyrophosphokinase."
    Baker L.-J., Dorocke J.A., Harris R.A., Timm D.E.
    Structure 9:539-546(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiTHI80_YEAST
AccessioniPrimary (citable) accession number: P35202
Secondary accession number(s): D6W2K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 14, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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