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P35202 (THI80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thiamine pyrophosphokinase
Short name=TPK
Short name=Thiamine kinase
EC=2.7.6.2
Gene names
Name:THI80
Ordered Locus Names:YOR143C
ORF Names:YOR3373C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

Catalytic activity

ATP + thiamine = AMP + thiamine diphosphate.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine: step 1/1.

Subunit structure

Homodimer.

Induction

Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the thiamine pyrophosphokinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Thiamine pyrophosphokinase
PRO_0000072513

Secondary structure

1 .............................................................. 319
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35202 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B82609E195D4D53F

FASTA31936,616
        10         20         30         40         50         60 
MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI PRPLFYKIWK 

        70         80         90        100        110        120 
LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS LSEKVYKYYR KNKVTIIKQT 

       130        140        150        160        170        180 
TQYSTDFTKC VNLISLHFNS PEFRSLISNK DNLQSNHGIE LEKGIHTLYN TMTESLVFSK 

       190        200        210        220        230        240 
VTPISLLALG GIGGRFDQTV HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD 

       250        260        270        280        290        300 
PQFRNTCIGN CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT 

       310 
KDDIILNVEI FVDKLIDFL

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae."
Nosaka K., Kaneko Y., Nishimura H., Iwashima A.
J. Biol. Chem. 268:17440-17447(1993) [PubMed: 8394343] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequencing and analysis of 130 kb from yeast chromosome XV."
Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W.
Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The crystal structure of yeast thiamin pyrophosphokinase."
Baker L.-J., Dorocke J.A., Harris R.A., Timm D.E.
Structure 9:539-546(2001) [PubMed: 11435118] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D14417 Genomic DNA. Translation: BAA03312.1.
X94335 Genomic DNA. Translation: CAA64061.1.
Z75051 Genomic DNA. Translation: CAA99346.1.
BK006948 Genomic DNA. Translation: DAA10917.1.
PIRA47499.
RefSeqNP_014786.1. NM_001183562.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IG0X-ray1.80A/B1-319[»]
ProteinModelPortalP35202.
SMRP35202. Positions 1-319.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5324N.
IntActP35202. 6 interactions.
MINTMINT-520646.
STRINGP35202.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR143C; YOR143C; YOR143C.
GeneID854314.
KEGGsce:YOR143C.
NMPDRfig|4932.3.peg.5891.

Organism-specific databases

CYGDYOR143c.
SGDS000005669. THI80.

Phylogenomic databases

eggNOGfuNOG09182.
GeneTreeEFGT00050000003518.
HOGENOMHBG397401.
OMAIGGRFDQ.
OrthoDBEOG4QVGN8.

Gene expression databases

ArrayExpressP35202.
GenevestigatorP35202.
GermOnlineYOR143C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR007371. TPK_catalytic.
[Graphical view]
Gene3DG3DSA:2.60.120.320. G3DSA:2.60.120.320. 1 hit.
G3DSA:3.40.50.10240. TPK_catalytic. 1 hit.
KOK00949.
PfamPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTSM00983. TPK_B1_binding. 1 hit.
[Graphical view]
SUPFAMSSF63862. TPK_B1_bd. 1 hit.
SSF63999. TPK_catalytic. 1 hit.
ProtoNetSearch...

Other

DrugBankDB00152. Thiamine.
NextBio976339.

Entry information

Entry nameTHI80_YEAST
AccessionPrimary (citable) accession number: P35202
Secondary accession number(s): D6W2K1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 25, 2012
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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