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Reviewed, UniProtKB/Swiss-Prot P35202 (THI80_YEAST)

Last modified November 25, 2008. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Thiamine pyrophosphokinase
      Short name=TPK
      Short name=Thiamine kinase
    EC=2.7.6.2
Gene names
Name: THI80
Ordered Locus Names: YOR143C
ORF Names: YOR3373C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Essential protein, it is the only enzyme in yeast capable of synthesizing thiamine pyrophosphate (TPP).

Catalytic activity

ATP + thiamine = AMP + thiamine diphosphate.

Pathway

Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; thiamine pyrophosphate from thiamine: step 1/1.

Subunit structure

Homodimer.

Induction

Expression requires the positive regulatory factors THI2 and THI3. Incompletely repressed by exogenous thiamine pyrophosphokinase.

Miscellaneous

Present with 3320 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the thiamine pyrophosphokinase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Thiamine pyrophosphokinase
PRO_0000072513

Secondary structure

1 .............................................................. 319
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P35202-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: B82609E195D4D53F

FASTA31936,616
        10         20         30         40         50         60 
MSEECIENPE RIKIGTDLIN IRNKMNLKEL IHPNEDENST LLILNQKIDI PRPLFYKIWK 

        70         80         90        100        110        120 
LHDLKVCADG AANRLYDYLD DDETLRIKYL PNYIIGDLDS LSEKVYKYYR KNKVTIIKQT 

       130        140        150        160        170        180 
TQYSTDFTKC VNLISLHFNS PEFRSLISNK DNLQSNHGIE LEKGIHTLYN TMTESLVFSK 

       190        200        210        220        230        240 
VTPISLLALG GIGGRFDQTV HSITQLYTLS ENASYFKLCY MTPTDLIFLI KKNGTLIEYD 

       250        260        270        280        290        300 
PQFRNTCIGN CGLLPIGEAT LVKETRGLKW DVKNWPTSVV TGRVSSSNRF VGDNCCFIDT 

       310 
KDDIILNVEI FVDKLIDFL

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References

« Hide 'large scale' references
[1]"Isolation and characterization of a thiamin pyrophosphokinase gene, THI80, from Saccharomyces cerevisiae."
Nosaka K., Kaneko Y., Nishimura H., Iwashima A.
J. Biol. Chem. 268:17440-17447(1993) [PubMed: 8394343] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequencing and analysis of 130 kb from yeast chromosome XV."
Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W.
Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"The crystal structure of yeast thiamin pyrophosphokinase."
Baker L.-J., Dorocke J.A., Harris R.A., Timm D.E.
Structure 9:539-546(2001) [PubMed: 11435118] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

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Cross-references

Sequence databases

D14417 Genomic DNA. Translation: BAA03312.1.
X94335 Genomic DNA. Translation: CAA64061.1.
Z75051 Genomic DNA. Translation: CAA99346.1.
PIRA47499.
RefSeqNP_014786.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1IG0X-ray1.80A/B1-319[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5324N.
IntActP35202.

Genome annotation databases

EnsemblYOR143C. Saccharomyces cerevisiae. [Contig view]
GeneID854314.
GenomeReviewsGene locus YOR143C in contig Y13140_GR.
KEGGsce:YOR143C.
NMPDRfig|4932.3.peg.5891.

Organism-specific databases

CYGDYOR143c.
SGDS000005669. THI80.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP35202.

Gene expression databases

GermOnlineYOR143C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. TPK_B1_bd.
IPR007371. TPK_catalytic.
[Graphical view]
PfamPF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
[Graphical view]
PIRSFPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
TIGRFAMsTIGR01378. thi_PPkinase. 1 hit.
ProtoNetSearch...

Other Resources

DrugBankDB00152. Thiamine.
LinkHubP35202.
NextBio976339.

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Entry information

Entry nameTHI80_YEAST
AccessionPrimary (citable) accession number: P35202
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 25, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents