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Protein

Protein MIF2

Gene

MIF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for mitotic spindle integrity during anaphase spindle elongation. Probably binds at tracts in DNA. Interacts with CEP1.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi356 – 3649A.T hook

GO - Molecular functioni

  • centromeric DNA binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • kinetochore assembly Source: InterPro
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle organization in nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31880-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MIF2
Gene namesi
Name:MIF2
Ordered Locus Names:YKL089W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL089W.
SGDiS000001572. MIF2.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: SGD
  • kinetochore Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Protein MIF2PRO_0000096486Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35201.

PTM databases

iPTMnetiP35201.

Interactioni

Protein-protein interaction databases

BioGridi34044. 62 interactions.
DIPiDIP-7297N.
IntActiP35201. 4 interactions.
MINTiMINT-433579.

Structurei

Secondary structure

1
549
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni447 – 4493Combined sources
Beta strandi453 – 4597Combined sources
Helixi463 – 4653Combined sources
Beta strandi467 – 4704Combined sources
Beta strandi473 – 48917Combined sources
Beta strandi492 – 4976Combined sources
Beta strandi501 – 5044Combined sources
Beta strandi509 – 5146Combined sources
Beta strandi516 – 5183Combined sources
Beta strandi520 – 5289Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VPVX-ray2.70A/B365-530[»]
ProteinModelPortaliP35201.
SMRiP35201. Positions 437-530.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35201.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi178 – 26386Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 A.T hook DNA-binding domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000001083.
InParanoidiP35201.
KOiK11497.
OMAiLAFWRNE.
OrthoDBiEOG092C26HV.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR025974. Mif2/CENP-C_cupin.
IPR028929. Mif2/cnp3_N.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 2 hits.
PfamiPF11699. CENP-C_C. 1 hit.
PF15624. Mif2_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYMKLGLKS RKTGIDVKQD IPKDEYSMEN IDDFFKDDET SLISMRRKSR
60 70 80 90 100
RKSSLFLPST LNGDTKNVLP PFLQSYKSQD DEVVQSPSGK GDGSRRSSLL
110 120 130 140 150
SHQSNFLSPA NDFEPIEEEP EQEENDIRGN DFATPITQKL SKPTYKRKYS
160 170 180 190 200
TRYSLDTSES PSVRLTPDRI TNKNVYSDVP DLVADEDDDD RVNTSLNTSD
210 220 230 240 250
NALLEDELED DGFIPESEED GDYIESDSSL DSGSDSASDS DGDNTYQEVE
260 270 280 290 300
EEAEVNTNDN EDDYIRRQAS DVVRTDSIID RNGLRKSTRV KVAPLQYWRN
310 320 330 340 350
EKIVYKRKSN KPVLDIDKIV TYDESEDEEE ILAAQRRKKQ KKKPTPTRPY
360 370 380 390 400
NYVPTGRPRG RPKKDPNAKE NLIPEDPNED IIERIESGGI ENGEWLKHGI
410 420 430 440 450
LEANVKISDT KEETKDEIIA FAPNLSQTEQ VKDTKDENFA LEIMFDKHKE
460 470 480 490 500
YFASGILKLP AISGQKKLSN SFRTYITFHV IQGIVEVTVC KNKFLSVKGS
510 520 530 540
TFQIPAFNEY AIANRGNDEA KMFFVQVTVS EDANDDNDKE LDSTFDTFG
Length:549
Mass (Da):62,473
Last modified:February 1, 1994 - v1
Checksum:iA6A4FAC9F6B66EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18294 Genomic DNA. Translation: CAA79163.1.
Z28089 Genomic DNA. Translation: CAA81927.1.
BK006944 Genomic DNA. Translation: DAA09069.1.
PIRiS37914.
RefSeqiNP_012834.1. NM_001179655.1.

Genome annotation databases

EnsemblFungiiYKL089W; YKL089W; YKL089W.
GeneIDi853773.
KEGGisce:YKL089W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18294 Genomic DNA. Translation: CAA79163.1.
Z28089 Genomic DNA. Translation: CAA81927.1.
BK006944 Genomic DNA. Translation: DAA09069.1.
PIRiS37914.
RefSeqiNP_012834.1. NM_001179655.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VPVX-ray2.70A/B365-530[»]
ProteinModelPortaliP35201.
SMRiP35201. Positions 437-530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34044. 62 interactions.
DIPiDIP-7297N.
IntActiP35201. 4 interactions.
MINTiMINT-433579.

PTM databases

iPTMnetiP35201.

Proteomic databases

MaxQBiP35201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL089W; YKL089W; YKL089W.
GeneIDi853773.
KEGGisce:YKL089W.

Organism-specific databases

EuPathDBiFungiDB:YKL089W.
SGDiS000001572. MIF2.

Phylogenomic databases

HOGENOMiHOG000001083.
InParanoidiP35201.
KOiK11497.
OMAiLAFWRNE.
OrthoDBiEOG092C26HV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31880-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP35201.
PROiP35201.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR028386. CENP-C/Mif2/cnp3.
IPR025974. Mif2/CENP-C_cupin.
IPR028929. Mif2/cnp3_N.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR16684. PTHR16684. 2 hits.
PfamiPF11699. CENP-C_C. 1 hit.
PF15624. Mif2_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIF2_YEAST
AccessioniPrimary (citable) accession number: P35201
Secondary accession number(s): D6VXJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 465 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.