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Protein

Inner kinetochore subunit MIF2

Gene

MIF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly (PubMed:8408221, PubMed:7579695, PubMed:22561346).3 Publications

Miscellaneous

Present with 465 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi356 – 364A.T hook9

GO - Molecular functioni

  • centromeric DNA binding Source: SGD

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: GO_Central
  • attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation Source: GO_Central
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • kinetochore assembly Source: GO_Central
  • mitotic spindle organization Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31880-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Inner kinetochore subunit MIF2Curated
Alternative name(s):
CENP-C homolog1 Publication
Constitutive centromere-associated network protein MIF2Curated
Mitotic fidelity of chromosome transmission protein 21 Publication
Gene namesi
Name:MIF2
Ordered Locus Names:YKL089W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL089W
SGDiS000001572 MIF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964861 – 549Inner kinetochore subunit MIF2Add BLAST549

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35201
PaxDbiP35201
PRIDEiP35201

PTM databases

iPTMnetiP35201

Interactioni

Subunit structurei

Component of the inner kinetochore constitutive centromere-associated network (CCAN) (also known as central kinetochore CTF19 complex in yeast), which is composed of at least AME1, CHL4, CNN1, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, MHF1, MHF2, MIF2, NKP1, NKP2, OKP1 and WIP1 (PubMed:22561346). Interacts with CBF1 (PubMed:7579695).2 Publications

Protein-protein interaction databases

BioGridi34044, 287 interactors
DIPiDIP-7297N
IntActiP35201, 4 interactors
MINTiP35201
STRINGi4932.YKL089W

Structurei

Secondary structure

1549
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni447 – 449Combined sources3
Beta strandi453 – 459Combined sources7
Helixi463 – 465Combined sources3
Beta strandi467 – 470Combined sources4
Beta strandi473 – 489Combined sources17
Beta strandi492 – 497Combined sources6
Beta strandi501 – 504Combined sources4
Beta strandi509 – 514Combined sources6
Beta strandi516 – 518Combined sources3
Beta strandi520 – 528Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VPVX-ray2.70A/B365-530[»]
ProteinModelPortaliP35201
SMRiP35201
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35201

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi178 – 263Asp/Glu-rich (acidic)Add BLAST86

Sequence similaritiesi

Belongs to the CENP-C/MIF2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000001083
InParanoidiP35201
KOiK11497
OMAiARIFFAQ
OrthoDBiEOG092C26HV

Family and domain databases

Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR028386 CENP-C/Mif2/cnp3
IPR025974 Mif2/CENP-C_cupin
IPR028929 Mif2/cnp3_N
IPR014710 RmlC-like_jellyroll
PANTHERiPTHR16684 PTHR16684, 2 hits
PfamiView protein in Pfam
PF11699 CENP-C_C, 1 hit
PF15624 Mif2_N, 1 hit

Sequencei

Sequence statusi: Complete.

P35201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYMKLGLKS RKTGIDVKQD IPKDEYSMEN IDDFFKDDET SLISMRRKSR
60 70 80 90 100
RKSSLFLPST LNGDTKNVLP PFLQSYKSQD DEVVQSPSGK GDGSRRSSLL
110 120 130 140 150
SHQSNFLSPA NDFEPIEEEP EQEENDIRGN DFATPITQKL SKPTYKRKYS
160 170 180 190 200
TRYSLDTSES PSVRLTPDRI TNKNVYSDVP DLVADEDDDD RVNTSLNTSD
210 220 230 240 250
NALLEDELED DGFIPESEED GDYIESDSSL DSGSDSASDS DGDNTYQEVE
260 270 280 290 300
EEAEVNTNDN EDDYIRRQAS DVVRTDSIID RNGLRKSTRV KVAPLQYWRN
310 320 330 340 350
EKIVYKRKSN KPVLDIDKIV TYDESEDEEE ILAAQRRKKQ KKKPTPTRPY
360 370 380 390 400
NYVPTGRPRG RPKKDPNAKE NLIPEDPNED IIERIESGGI ENGEWLKHGI
410 420 430 440 450
LEANVKISDT KEETKDEIIA FAPNLSQTEQ VKDTKDENFA LEIMFDKHKE
460 470 480 490 500
YFASGILKLP AISGQKKLSN SFRTYITFHV IQGIVEVTVC KNKFLSVKGS
510 520 530 540
TFQIPAFNEY AIANRGNDEA KMFFVQVTVS EDANDDNDKE LDSTFDTFG
Length:549
Mass (Da):62,473
Last modified:February 1, 1994 - v1
Checksum:iA6A4FAC9F6B66EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18294 Genomic DNA Translation: CAA79163.1
Z28089 Genomic DNA Translation: CAA81927.1
BK006944 Genomic DNA Translation: DAA09069.1
PIRiS37914
RefSeqiNP_012834.1, NM_001179655.1

Genome annotation databases

EnsemblFungiiYKL089W; YKL089W; YKL089W
GeneIDi853773
KEGGisce:YKL089W

Similar proteinsi

Entry informationi

Entry nameiCENPC_YEAST
AccessioniPrimary (citable) accession number: P35201
Secondary accession number(s): D6VXJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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