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Protein

Dehydrodolichyl diphosphate synthase complex subunit RER2

Gene

RER2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

With NUS1, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Adds multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER).2 Publications

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10-55).2 Publications

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dehydrodolichyl diphosphate synthase activity Source: SGD
  • polyprenyltransferase activity Source: GO_Central
  • prenyltransferase activity Source: SGD

GO - Biological processi

  • dolichol biosynthetic process Source: SGD
  • ER to Golgi vesicle-mediated transport Source: SGD
  • protein glycosylation Source: SGD

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:YBR002C-MONOMER.
YEAST:YBR002C-MONOMER.
BRENDAi2.5.1.87. 984.
ReactomeiR-SCE-446199. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrodolichyl diphosphate synthase complex subunit RER2Curated (EC:2.5.1.872 Publications)
Short name:
DEDOL-PP synthaseCurated
Alternative name(s):
Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific)Curated
Gene namesi
Name:RER2Imported
Ordered Locus Names:YBR002CImported
ORF Names:YBR0107
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR002C.
SGDiS000000206. RER2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164G → D in RER2-1; loss of activity. 1
Mutagenesisi209S → N in RER2-2; loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001237591 – 286Dehydrodolichyl diphosphate synthase complex subunit RER2Add BLAST286

Proteomic databases

MaxQBiP35196.
PRIDEiP35196.

Expressioni

Inductioni

Mainly expressed in the early logarithmic phase.1 Publication

Interactioni

Subunit structurei

Forms an active dehydrodolichyl diphosphate synthase complex with NUS1.1 Publication

Protein-protein interaction databases

BioGridi32703. 377 interactors.
IntActiP35196. 26 interactors.
MINTiMINT-4476587.
STRINGi4932.YBR002C.

Structurei

3D structure databases

ProteinModelPortaliP35196.
SMRiP35196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UPP synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000007879.
HOGENOMiHOG000006053.
InParanoidiP35196.
KOiK11778.
OMAiTKGQPDP.
OrthoDBiEOG092C3WY3.

Family and domain databases

CDDicd00475. Cis_IPPS. 1 hit.
Gene3Di3.40.1180.10. 1 hit.
HAMAPiMF_01139. ISPT. 1 hit.
InterProiView protein in InterPro
IPR001441. UPP_synth-like.
IPR018520. UPP_synth-like_CS.
PANTHERiPTHR10291. PTHR10291. 1 hit.
PfamiView protein in Pfam
PF01255. Prenyltransf. 1 hit.
SUPFAMiSSF64005. SSF64005. 1 hit.
TIGRFAMsiTIGR00055. uppS. 1 hit.
PROSITEiView protein in PROSITE
PS01066. UPP_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P35196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METDSGIPGH SFVLKWTKNI FSRTLRASNC VPRHVGFIMD GNRRFARKKE
60 70 80 90 100
MDVKEGHEAG FVSMSRILEL CYEAGVDTAT VFAFSIENFK RSSREVESLM
110 120 130 140 150
TLARERIRQI TERGELACKY GVRIKIIGDL SLLDKSLLED VRVAVETTKN
160 170 180 190 200
NKRATLNICF PYTGREEILH AMKETIVQHK KGAAIDESTL ESHLYTAGVP
210 220 230 240 250
PLDLLIRTSG VSRLSDFLIW QASSKGVRIE LLDCLWPEFG PIRMAWILLK
260 270 280
FSFHKSFLNK EYRLEEGDYD EETNGDPIDL KEKKLN
Length:286
Mass (Da):32,694
Last modified:February 1, 1994 - v1
Checksum:i73F27FC9EED61F4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013497 Genomic DNA. Translation: BAA36577.1.
Z26494 Genomic DNA. Translation: CAA81271.1.
Z35871 Genomic DNA. Translation: CAA84938.1.
BK006936 Genomic DNA. Translation: DAA07123.1.
PIRiS44561.
RefSeqiNP_009556.1. NM_001178350.1.

Genome annotation databases

EnsemblFungiiYBR002C; YBR002C; YBR002C.
GeneIDi852287.
KEGGisce:YBR002C.

Similar proteinsi

Entry informationi

Entry nameiRER2_YEAST
AccessioniPrimary (citable) accession number: P35196
Secondary accession number(s): D6VQ03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 27, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names