P35194 (UTP20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U3 small nucleolar RNA-associated protein 20
Short name=U3 snoRNA-associated protein 20

Alternative name(s):
U three protein 20

Gene names

Name:	UTP20
Ordered Locus Names:	YBL004W
ORF Names:	YBL0101

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	2493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.5
Subunit structure	Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.5
Subcellular location	Cytoplasm. Nucleus › nucleolus Ref.4 Ref.5.
Sequence similarities	Belongs to the UTP20 family. Contains 16 HEAT repeats.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Ribonucleoprotein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.5PubMed 17652137. Source: SGD endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.5PubMed 17652137. Source: SGD endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype Ref.5PubMed 17652137. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911PubMed 17652137. Source: SGD cytoplasm Inferred from direct assay Ref.4PubMed 17652137. Source: SGD nucleolus Inferred from direct assay Ref.4Ref.5PubMed 17652137. Source: SGD nucleoplasm Inferred from direct assay PubMed 17652137. Source: SGD preribosome, small subunit precursor Inferred from direct assay PubMed 17652137. Source: SGD small-subunit processome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2493	2493	U3 small nucleolar RNA-associated protein 20		PRO_0000202465
Regions
	Repeat	227 – 264	38	HEAT 1
	Repeat	495 – 532	38	HEAT 2
	Repeat	576 – 613	38	HEAT 3
	Repeat	845 – 882	38	HEAT 4
	Repeat	1176 – 1214	39	HEAT 5
	Repeat	1216 – 1252	37	HEAT 6
	Repeat	1342 – 1380	39	HEAT 7
	Repeat	1393 – 1430	38	HEAT 8
	Repeat	1480 – 1520	41	HEAT 9
	Repeat	1522 – 1558	37	HEAT 10
	Repeat	1588 – 1625	38	HEAT 11
	Repeat	1630 – 1667	38	HEAT 12
	Repeat	1890 – 1927	38	HEAT 13
	Repeat	1953 – 1992	40	HEAT 14
	Repeat	2120 – 2157	38	HEAT 15
	Repeat	2358 – 2397	40	HEAT 16
Amino acid modifications
	Modified residue	1577	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	2440	1	R → S in CAA81266. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P35194 [UniParc]. Last modified May 15, 2007. Version 3. Checksum: F6ED4E3E9AE0F468	FASTA	2,493	287,560
	10 20 30 40 50 60 MAKQRQTTKS SKRYRYSSFK ARIDDLKIEP ARNLEKRVHD YVESSHFLAS FDQWKEINLS 70 80 90 100 110 120 AKFTEFAAEI EHDVQTLPQI LYHDKKIFNS LVSFINFHDE FSLQPLLDLL AQFCHDLGPD 130 140 150 160 170 180 FLKFYEEAIK TLINLLDAAI EFESSNVFEW GFNCLAYIFK YLSKFLVKKL VLTCDLLIPL 190 200 210 220 230 240 LSHSKEYLSR FSAEALSFLV RKCPVSNLRE FVRSVFEKLE GDDEQTNLYE GLLILFTESM 250 260 270 280 290 300 TSTQETLHSK AKAIMSVLLH EALTKSSPER SVSLLSDIWM NISKYASIES LLPVYEVMYQ 310 320 330 340 350 360 DFNDSLDATN IDRILKVLTT IVFSESGRKI PDWNKITILI ERIMSQSENC ASLSQDKVAF 370 380 390 400 410 420 LFALFIRNSD VKTLTLFHQK LFNYALTNIS DCFLEFFQFA LRLSYERVFS FNGLKFLQLF 430 440 450 460 470 480 LKKNWQSQGK KIALFFLEVD DKPELQKVRE VNFPEEFILS IRDFFVTAEI NDSNDLFEIY 490 500 510 520 530 540 WRAIIFKYSK LQNTEIIIPL LERIFSTFAS PDNFTKDMVG TLLKIYRKED DASGNNLLKT 550 560 570 580 590 600 ILDNYENYKE SLNFLRGWNK LVSNLHPSES LKGLMSHYPS LLLSLTDNFM LPDGKIRYET 610 620 630 640 650 660 LELMKTLMIL QGMQVPDLLS SCMVIEEIPL TLQNARDLTI RIKNVGAEFG KTKTDKLVSS 670 680 690 700 710 720 FFLKYLFGLL TVRFSPVWTG VFDTLPNVYT KDEALVWKLV LSFIKLPDEN QNLDYYQPLL 730 740 750 760 770 780 EDGANKVLWD SSVVRLRDTI DTFSHIWSKY STQNTSIIST TIERRGNTTY PILIRNQALK 790 800 810 820 830 840 VMLSIPQVAE NHFVDIAPFV YNDFKTYKDE EDMENERVIT GSWTEVDRNV FLKTLSKFKN 850 860 870 880 890 900 IKNVYSATEL HDHLMVLLGS RNTDVQKLAL DALLAYKNPT LNKYRDNLKN LLDDTLFKDE 910 920 930 940 950 960 ITTFLTENGS QSIKAEDEKV VMPYVLRIFF GRAQVPPTSG QKRSRKIAVI SVLPNFKKPY 970 980 990 1000 1010 1020 INDFLSLASE RLDYNYFFGN SHQINSSKAT LKTIRRMTGF VNIVNSTLSV LRTNFPLHTN 1030 1040 1050 1060 1070 1080 SVLQPLIYSI AMAYYVLDTE STEEVHLRKM ASNLRQQGLK CLSSVFEFVG NTFDWSTSME 1090 1100 1110 1120 1130 1140 DIYAVVVKPR ISHFSDENLQ QPSSLLRLFL YWAHNPSLYQ FLYYDEFATA TALMDTISNQ 1150 1160 1170 1180 1190 1200 HVKEAVIGPI IEAADSIIRN PVNDDHYVDL VTLICTSCLK ILPSLYVKLS DSNSISTFLN 1210 1220 1230 1240 1250 1260 LLVSITEMGF IQDDHVRSRL ISSLISILKG KLKKLQENDT QKILKILKLI VFNYNCSWSD 1270 1280 1290 1300 1310 1320 IEELYTTISS LFKTFDERNL RVSLTELFIE LGRKVPELES ISKLVADLNS YSSSRMHEYD 1330 1340 1350 1360 1370 1380 FPRILSTFKG LIEDGYKSYS ELEWLPLLFT FLHFINNKEE LALRTNASHA IMKFIDFINE 1390 1400 1410 1420 1430 1440 KPNLNEASKS ISMLKDILLP NIRIGLRDSL EEVQSEYVSV LSYMVKNTKY FTDFEDMAIL 1450 1460 1470 1480 1490 1500 LYNGDEEADF FTNVNHIQLH RRQRAIKRLG EHAHQLKDNS ISHYLIPMIE HYVFSDDERY 1510 1520 1530 1540 1550 1560 RNIGNETQIA IGGLAQHMSW NQYKALLRRY ISMLKTKPNQ MKQAVQLIVQ LSVPLRETLR 1570 1580 1590 1600 1610 1620 IVRDGAESKL TLSKFPSNLD EPSNFIKQEL YPTLSKILGT RDDETIIERM PIAEALVNIV 1630 1640 1650 1660 1670 1680 LGLTNDDITN FLPSILTNIC QVLRSKSEEL RDAVRVTLGK ISIILGAEYL VFVIKELMAT 1690 1700 1710 1720 1730 1740 LKRGSQIHVL SYTVHYILKS MHGVLKHSDL DTSSSMIVKI IMENIFGFAG EEKDSENYHT 1750 1760 1770 1780 1790 1800 KVKEIKSNKS YDAGEILASN ISLTEFGTLL SPVKALLMVR INLRNQNKLS ELLRRYLLGL 1810 1820 1830 1840 1850 1860 NHNSDSESES ILKFCHQLFQ ESEMSNSPQI PKKKVKDQVD EKEDFFLVNL ESKSYTINSN 1870 1880 1890 1900 1910 1920 SLLLNSTLQK FALDLLRNVI TRHRSFLTVS HLEGFIPFLR DSLLSENEGV VISTLRILIT 1930 1940 1950 1960 1970 1980 LIRLDFSDES SEIFKNCARK VLNIIKVSPS TSSELCQMGL KFLSAFIRHT DSTLKDTALS 1990 2000 2010 2020 2030 2040 YVLGRVLPDL NEPSRQGLAF NFLKALVSKH IMLPELYDIA DTTREIMVTN HSKEIRDVSR 2050 2060 2070 2080 2090 2100 SVYYQFLMEY DQSKGRLEKQ FKFMVDNLQY PTESGRQSVM ELINLIITKA NPALLSKLSS 2110 2120 2130 2140 2150 2160 SFFLALVNVS FNDDAPRCRE MASVLISTML PKLENKDLEI VEKYIAAWLK QVDNASFLNL 2170 2180 2190 2200 2210 2220 GLRTYKVYLK SIGFEHTIEL DELAIKRIRY ILSDTSVGSE HQWDLVYSAL NTFSSYMEAT 2230 2240 2250 2260 2270 2280 ESVYKHGFKD IWDGIITCLL YPHSWVRQSA ANLVHQLIAN KDKLEISLTN LEIQTIATRI 2290 2300 2310 2320 2330 2340 LHQLGAPSIP ENLANVSIKT LVNISILWKE QRTPFIMDVS KQTGEDLKYT TAIDYMVTRI 2350 2360 2370 2380 2390 2400 GGIIRSDEHR MDSFMSKKAC IQLLALLVQV LDEDEVIAEG EKILLPLYGY LETYYSRAVD 2410 2420 2430 2440 2450 2460 EEQEELRTLS NECLKILEDK LQVSDFTKIY TAVKQTVLER RKERRSKRAI LAVNAPQISA 2470 2480 2490 DKKLRKHARS REKRKHEKDE NGYYQRRNKR KRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Sequence around the centromere of Saccharomyces cerevisiae chromosome II: similarity of CEN2 to CEN4." Wolfe K.H., Lohan A.J.E. Yeast 10:S41-S46(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1214-2493. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"The small-subunit processome is a ribosome assembly intermediate." Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J. Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1577, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35765 Genomic DNA. Translation: CAA84821.1. Z26494 Genomic DNA. Translation: CAA81266.1. BK006936 Genomic DNA. Translation: DAA07118.1.
PIR	S45734.
RefSeq	NP_009551.2. NM_001178244.1.
3D structure databases
ProteinModelPortal	P35194.
SMR	P35194. Positions 1399-1424, 1587-1663, 1891-1923.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2826N.
IntAct	P35194. 38 interactions.
MINT	MINT-669095.
STRING	4932.YBL004W.
Proteomic databases
PaxDb	P35194.
PeptideAtlas	P35194.
PRIDE	P35194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL004W; YBL004W; YBL004W.
GeneID	852282.
KEGG	sce:YBL004W.
Organism-specific databases
SGD	S000000100. UTP20.
Phylogenomic databases
eggNOG	NOG296791.
GeneTree	ENSGT00390000016813.
HOGENOM	HOG000248819.
KO	K14772.
OMA	GASERIM.
OrthoDB	EOG4KH637.
Gene expression databases
Genevestigator	P35194.
GermOnline	YBL004W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 12 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR011430. DRIM. [Graphical view]
Pfam	PF07539. DRIM. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 2 hits.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	970910.

Entry information

Entry name

UTP20_YEAST

Accession

Primary (citable) accession number: P35194
Secondary accession number(s): D6VPZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 15, 2007
Last modified:	May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program