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Protein

Autophagy-related protein 19

Gene

ATG19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cargo-receptor protein involved in the cytoplasm to vacuole transport (Cvt) and in autophagy. Recognizes cargo proteins, such as APE4, LAP3, LAP4 and AMS1 and delivers them to the pre-autophagosomal structure for eventual engulfment by the autophagosome and targeting to the vacuole. Involved in the organization of the preautophagosomal structure (PAS). ATG19 association with cargo protein is required to localize ATG11 to the PAS. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates, and is essential for the survival of cells subjected to severe ER stress. Plays also a role in regulation of filamentous growth.16 Publications

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • CVT pathway Source: SGD
  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • protein complex localization Source: SGD
  • protein processing Source: SGD
  • vesicle organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33485-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 19
Alternative name(s):
Cytoplasm-to-vacuole targeting protein 19
Gene namesi
Name:ATG19
Synonyms:CVT19
Ordered Locus Names:YOL082W
ORF Names:O0980, YOL01
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL082W.
SGDiS000005442. ATG19.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • extrinsic component of membrane Source: SGD
  • pre-autophagosomal structure Source: SGD
  • pre-autophagosomal structure membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Biotechnological usei

ATG19 is important for yeast autolysis which is the source of several molecules responsible for the quality of wines aged in contact with yeast cells.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi412W → A: Impairs vacuolar transport of LAP4. 1 Publication1
Mutagenesisi415L → A: Impairs vacuolar transport of LAP4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000647221 – 415Autophagy-related protein 19Add BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei136PhosphoserineCombined sources1
Modified residuei141PhosphoserineCombined sources1
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei243PhosphoserineCombined sources1

Post-translational modificationi

Polyubiquitinated at Lys-213 and Lys-216. Deubiquitination by UBP3 is required for full activity of ATG19.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP35193.
PRIDEiP35193.

PTM databases

iPTMnetiP35193.

Interactioni

Subunit structurei

Interacts with the vacuolar aminopeptidase 1 (LAP4) precursor and mature forms. Also interacts with AMS1, APE4, ATG8 ATG11, and UBP3.12 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG1P531042EBI-29291,EBI-2657
ATG11Q125274EBI-29291,EBI-31977
HRR25P292952EBI-29291,EBI-8536

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34320. 66 interactors.
DIPiDIP-1407N.
IntActiP35193. 22 interactors.
MINTiMINT-387666.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi161 – 184Combined sources24
Beta strandi262 – 269Combined sources8
Beta strandi272 – 279Combined sources8
Beta strandi281 – 283Combined sources3
Beta strandi295 – 299Combined sources5
Beta strandi318 – 322Combined sources5
Beta strandi335 – 338Combined sources4
Beta strandi342 – 348Combined sources7
Beta strandi352 – 354Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KZBNMR-A254-367[»]
2ZPNX-ray2.70E/F/G/H412-415[»]
5JGEX-ray1.91A/B/D/E160-187[»]
ProteinModelPortaliP35193.
SMRiP35193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35193.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 28ATG11-binding8
Regioni254 – 367AMS1-bindingAdd BLAST114
Regioni406 – 415ATG8-binding10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili157 – 187Sequence analysisAdd BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi412 – 415WXXL4

Domaini

The coiled coil domain is required for the interaction with LAP4.
The C-terminal WXXL motif is crucial for ATG8-binding and Cvt pathway.

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00530000068761.
InParanoidiP35193.
OrthoDBiEOG092C2GAB.

Family and domain databases

InterProiIPR024543. Atg19/Atg34_C.
[Graphical view]
PfamiPF12744. ATG19_autophagy. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSKTNQQM NTSMGYPLTV YDECNKFQLI VPTLDANIML WCIGQLSLLN
60 70 80 90 100
DSNGCKHLFW QPNDKSNVRI LLNNYDYGHL FKYLQCQRKC SVYIGEGTLK
110 120 130 140 150
KYNLTISTSF DNFLDLTPSE EKESLCREDA HEDPVSPKAG SEEEISPNST
160 170 180 190 200
SNVVVSRECL DNFMKQLLKL EESLNKLELE QKVTNKEPNH RISGTIDIPE
210 220 230 240 250
DRSELVNFFT ELKTVKQLED VFQRYHDYER LSQECDSKTE IASDHSKKET
260 270 280 290 300
KIEVEPPNER SLQITMNQRD NSLYFQLFNN TNSVLAGNCK LKFTDAGDKP
310 320 330 340 350
TTQIIDMGPH EIGIKEYKEY RYFPYALDLE AGSTIEIENQ YGEVIFLGKY
360 370 380 390 400
GSSPMINLRP PSRLSAESLQ ASQEPFYSFQ IDTLPELDDS SIISTSISLS
410
YDGDDNEKAL TWEEL
Length:415
Mass (Da):47,599
Last modified:February 1, 1994 - v1
Checksum:iB49DA1DF83A04070
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti412W → R in AAT93193 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75449 Genomic DNA. Translation: CAA53201.1.
X83121 Genomic DNA. Translation: CAA58199.1.
Z74824 Genomic DNA. Translation: CAA99094.1.
AY693174 Genomic DNA. Translation: AAT93193.1.
BK006948 Genomic DNA. Translation: DAA10702.1.
PIRiS48253.
RefSeqiNP_014559.1. NM_001183336.1.

Genome annotation databases

EnsemblFungiiYOL082W; YOL082W; YOL082W.
GeneIDi854072.
KEGGisce:YOL082W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75449 Genomic DNA. Translation: CAA53201.1.
X83121 Genomic DNA. Translation: CAA58199.1.
Z74824 Genomic DNA. Translation: CAA99094.1.
AY693174 Genomic DNA. Translation: AAT93193.1.
BK006948 Genomic DNA. Translation: DAA10702.1.
PIRiS48253.
RefSeqiNP_014559.1. NM_001183336.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KZBNMR-A254-367[»]
2ZPNX-ray2.70E/F/G/H412-415[»]
5JGEX-ray1.91A/B/D/E160-187[»]
ProteinModelPortaliP35193.
SMRiP35193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34320. 66 interactors.
DIPiDIP-1407N.
IntActiP35193. 22 interactors.
MINTiMINT-387666.

PTM databases

iPTMnetiP35193.

Proteomic databases

MaxQBiP35193.
PRIDEiP35193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL082W; YOL082W; YOL082W.
GeneIDi854072.
KEGGisce:YOL082W.

Organism-specific databases

EuPathDBiFungiDB:YOL082W.
SGDiS000005442. ATG19.

Phylogenomic databases

GeneTreeiENSGT00530000068761.
InParanoidiP35193.
OrthoDBiEOG092C2GAB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33485-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP35193.
PROiP35193.

Family and domain databases

InterProiIPR024543. Atg19/Atg34_C.
[Graphical view]
PfamiPF12744. ATG19_autophagy. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATG19_YEAST
AccessioniPrimary (citable) accession number: P35193
Secondary accession number(s): D6W1Y6, Q6B1A6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.