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Protein

DnaJ homolog 1, mitochondrial

Gene

MDJ1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mitochondrial biogenesis and protein folding.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri217 – 29781CR-typeAdd
BLAST

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • ATP binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • unfolded protein binding Source: SGD

GO - Biological processi

  • 'de novo' protein folding Source: SGD
  • misfolded or incompletely synthesized protein catabolic process Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • positive regulation of ATPase activity Source: GOC
  • protein refolding Source: SGD
  • response to heat Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30441-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog 1, mitochondrial
Gene namesi
Name:MDJ1
Ordered Locus Names:YFL016C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL016C.
SGDiS000001878. MDJ1.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of mitochondrial inner membrane Source: SGD
  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5555MitochondrionSequence analysisAdd
BLAST
Chaini56 – 511456DnaJ homolog 1, mitochondrialPRO_0000007268Add
BLAST

Proteomic databases

MaxQBiP35191.
PeptideAtlasiP35191.

Interactioni

GO - Molecular functioni

  • unfolded protein binding Source: SGD

Protein-protein interaction databases

BioGridi31131. 55 interactions.
DIPiDIP-764N.
IntActiP35191. 34 interactions.
MINTiMINT-475767.

Structurei

3D structure databases

ProteinModelPortaliP35191.
SMRiP35191. Positions 58-174, 203-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 12769JAdd
BLAST
Repeati230 – 2378CXXCXGXG motif
Repeati247 – 2548CXXCXGXG motif
Repeati269 – 2768CXXCXGXG motif
Repeati285 – 2928CXXCXGXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi130 – 18657Gly-richAdd
BLAST

Sequence similaritiesi

Contains 1 CR-type zinc finger.Curated
Contains 1 J domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri217 – 29781CR-typeAdd
BLAST

Keywords - Domaini

Repeat, Transit peptide, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00840000129833.
HOGENOMiHOG000226717.
InParanoidiP35191.
KOiK03686.
OMAiVISIPNM.
OrthoDBiEOG744TK7.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQQGVLSR CSGVFRHHVG HSRHINNILY RHAIAFASIA PRIPKSSFHT
60 70 80 90 100
SAIRNNEAFK DPYDTLGLKK SATGAEIKKA YYKLAKKYHP DINKEPDAEK
110 120 130 140 150
KFHDLQNAYE ILSDETKRQQ YDQFGPAAFG GGGAAGGAGG GSGSPFGSQF
160 170 180 190 200
HDFSGFTSAG GSPFGGINFE DLFGAAFGGG GRGSGGASRS SSMFRQYRGD
210 220 230 240 250
PIEIVHKVSF KDAVFGSKNV QLRFSALDPC STCSGTGMKP NTHKVSCSTC
260 270 280 290 300
HGTGTTVHIR GGFQMMSTCP TCNGEGTMKR PQDNCTKCHG EGVQVNRAKT
310 320 330 340 350
ITVDLPHGLQ DGDVVRIPGQ GSYPDIAVEA DLKDSVKLSR GDILVRIRVD
360 370 380 390 400
KDPNFSIKNK YDIWYDKEIP ITTAALGGTV TIPTVEGQKI RIKVAPGTQY
410 420 430 440 450
NQVISIPNMG VPKTSTIRGD MKVQYKIVVK KPQSLAEKCL WEALADVTND
460 470 480 490 500
DMAKKTMQPG TAAGTAINEE ILKKQKQEEE KHAKKDDDNT LKRLENFITN
510
TFRKIKGDKK N
Length:511
Mass (Da):55,561
Last modified:February 1, 1994 - v1
Checksum:i007343427C66D2B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28336 Genomic DNA. Translation: CAA82189.1.
Z46255 Genomic DNA. Translation: CAA86351.1.
D50617 Genomic DNA. Translation: BAA09222.1.
BK006940 Genomic DNA. Translation: DAA12424.1.
PIRiS38898.
RefSeqiNP_116638.1. NM_001179950.1.

Genome annotation databases

EnsemblFungiiBAA09222; BAA09222; BAA09222.
YFL016C; YFL016C; YFL016C.
GeneIDi850530.
KEGGisce:YFL016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28336 Genomic DNA. Translation: CAA82189.1.
Z46255 Genomic DNA. Translation: CAA86351.1.
D50617 Genomic DNA. Translation: BAA09222.1.
BK006940 Genomic DNA. Translation: DAA12424.1.
PIRiS38898.
RefSeqiNP_116638.1. NM_001179950.1.

3D structure databases

ProteinModelPortaliP35191.
SMRiP35191. Positions 58-174, 203-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31131. 55 interactions.
DIPiDIP-764N.
IntActiP35191. 34 interactions.
MINTiMINT-475767.

Proteomic databases

MaxQBiP35191.
PeptideAtlasiP35191.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09222; BAA09222; BAA09222.
YFL016C; YFL016C; YFL016C.
GeneIDi850530.
KEGGisce:YFL016C.

Organism-specific databases

EuPathDBiFungiDB:YFL016C.
SGDiS000001878. MDJ1.

Phylogenomic databases

GeneTreeiENSGT00840000129833.
HOGENOMiHOG000226717.
InParanoidiP35191.
KOiK03686.
OMAiVISIPNM.
OrthoDBiEOG744TK7.

Enzyme and pathway databases

BioCyciYEAST:G3O-30441-MONOMER.

Miscellaneous databases

NextBioi966277.
PROiP35191.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Rowley N.K., Prip-Buus C., Westermann B., Brown C.M., Schwarz E., Barrell B.G., Neupert W.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Barrell B.G., Churcher C., Rajandream M.A.
    Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding."
    Rowley N., Prip-Buus C., Westermann B., Brown C., Schwarz E., Barrell B., Neupert W.
    Cell 77:249-259(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Purification and biochemical properties of Saccharomyces cerevisiae Mdj1p, the mitochondrial DnaJ homologue."
    Deloche O., Liberek K., Zylicz M., Georgopoulos C.
    J. Biol. Chem. 272:28539-28544(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMDJ1_YEAST
AccessioniPrimary (citable) accession number: P35191
Secondary accession number(s): D6VTL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 11, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7570 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.