DnaJ homolog 1, mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P35191 (MDJ1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DnaJ homolog 1, mitochondrial

Gene names

Name:	MDJ1
Ordered Locus Names:	YFL016C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in mitochondrial biogenesis and protein folding. Ref.5 Ref.6
Subcellular location	Mitochondrion.
Miscellaneous	Present with 7570 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 CR-type zinc finger. Contains 1 J domain.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Mitochondrion
Domain	Repeat Transit peptide Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' protein folding Inferred from mutant phenotype Ref.5. Source: SGD misfolded or incompletely synthesized protein catabolic process Inferred from mutant phenotype. Source: SGD mitochondrial genome maintenance Inferred from mutant phenotype. Source: SGD protein refolding Inferred from direct assay. Source: SGD response to heat Inferred from mutant phenotype. Source: SGD
Cellular component	extrinsic to mitochondrial inner membrane Inferred from direct assay Ref.5. Source: SGD mitochondrial matrix Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activator activity Inferred from direct assay. Source: SGD heat shock protein binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 55

Mitochondrion Potential

Chain

56 – 511

456

DnaJ homolog 1, mitochondrial

PRO_0000007268

Regions

Domain

59 – 127

Repeat

230 – 237

CXXCXGXG motif

Repeat

247 – 254

CXXCXGXG motif

Repeat

269 – 276

CXXCXGXG motif

Repeat

285 – 292

CXXCXGXG motif

Zinc finger

217 – 297

CR-type

Compositional bias

130 – 186

Gly-rich

Sequences

Sequence

Length

Mass (Da)

Tools

P35191 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 007343427C66D2B6
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FASTA

511

55,561

        10         20         30         40         50         60 
MAFQQGVLSR CSGVFRHHVG HSRHINNILY RHAIAFASIA PRIPKSSFHT SAIRNNEAFK 

        70         80         90        100        110        120 
DPYDTLGLKK SATGAEIKKA YYKLAKKYHP DINKEPDAEK KFHDLQNAYE ILSDETKRQQ 

       130        140        150        160        170        180 
YDQFGPAAFG GGGAAGGAGG GSGSPFGSQF HDFSGFTSAG GSPFGGINFE DLFGAAFGGG 

       190        200        210        220        230        240 
GRGSGGASRS SSMFRQYRGD PIEIVHKVSF KDAVFGSKNV QLRFSALDPC STCSGTGMKP 

       250        260        270        280        290        300 
NTHKVSCSTC HGTGTTVHIR GGFQMMSTCP TCNGEGTMKR PQDNCTKCHG EGVQVNRAKT 

       310        320        330        340        350        360 
ITVDLPHGLQ DGDVVRIPGQ GSYPDIAVEA DLKDSVKLSR GDILVRIRVD KDPNFSIKNK 

       370        380        390        400        410        420 
YDIWYDKEIP ITTAALGGTV TIPTVEGQKI RIKVAPGTQY NQVISIPNMG VPKTSTIRGD 

       430        440        450        460        470        480 
MKVQYKIVVK KPQSLAEKCL WEALADVTND DMAKKTMQPG TAAGTAINEE ILKKQKQEEE 

       490        500        510 
KHAKKDDDNT LKRLENFITN TFRKIKGDKK N

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Rowley N.K., Prip-Buus C., Westermann B., Brown C.M., Schwarz E., Barrell B.G., Neupert W. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Barrell B.G., Churcher C., Rajandream M.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding." Rowley N., Prip-Buus C., Westermann B., Brown C., Schwarz E., Barrell B., Neupert W. Cell 77:249-259(1994) [PubMed: 8168133] [Abstract] Cited for: FUNCTION.
[6]	"Purification and biochemical properties of Saccharomyces cerevisiae Mdj1p, the mitochondrial DnaJ homologue." Deloche O., Liberek K., Zylicz M., Georgopoulos C. J. Biol. Chem. 272:28539-28544(1997) [PubMed: 9353316] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28336 Genomic DNA. Translation: CAA82189.1. Z46255 Genomic DNA. Translation: CAA86351.1. D50617 Genomic DNA. Translation: BAA09222.1. BK006940 Genomic DNA. Translation: DAA12424.1.
PIR	S38898.
RefSeq	NP_116638.1. NM_001179950.1.
3D structure databases
ProteinModelPortal	P35191.
SMR	P35191. Positions 59-128, 196-431.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-764N.
IntAct	P35191. 36 interactions.
MINT	MINT-475767.
STRING	P35191.
Proteomic databases
PeptideAtlas	P35191.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL016C; YFL016C; YFL016C.
GeneID	850530.
KEGG	sce:YFL016C.
NMPDR	fig\|4932.3.peg.2266.
Organism-specific databases
CYGD	YFL016c.
SGD	S000001878. MDJ1.
Phylogenomic databases
eggNOG	fuNOG04758.
GeneTree	EFGT00050000001935.
HOGENOM	HBG635315.
OMA	FARMKEF.
OrthoDB	EOG4KM2BK.
Gene expression databases
ArrayExpress	P35191.
Genevestigator	P35191.
GermOnline	YFL016C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012724. DnaJ. IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR003095. Hsp_DnaJ. IPR001305. HSP_DnaJ_Cys-rich_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:2.10.230.10. HSP_DnaJ_cys-rich. 1 hit.
KO	K03686.
PANTHER	PTHR24076:SF80. PTHR24076:SF80. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. PF00684. DnaJ_CXXCXGXG. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit. SSF49493. HSP40_DnaJ_pep. 2 hits. SSF57938. HSP_DnaJ_cys-rich. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51188. ZF_CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966277.

Entry information

Entry name

MDJ1_YEAST

Accession

Primary (citable) accession number: P35191
Secondary accession number(s): D6VTL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents