ATP-dependent helicase SGS1 - Saccharomyces cerevisiae (Baker's yeast)

Sequence

>sp|P35187|SGS1_YEAST ATP-dependent helicase SGS1 OS=Saccharomyces cerevisiae GN=SGS1 PE=1 SV=1 MVTKPSHNLRREHKWLKETATLQEDKDFVFQAIQKHIANKRPKTNSPPTTPSKDECGPGT TNFITSIPASGPTNTATKQHEVMQTLSNDTEWLSYTATSNQYADVPMVDIPASTSVVSNP RTPNGSKTHNFNTFRPHMASSLVENDSSRNLGSRNNNKSVIDNSSIGKQLENDIKLEVIR LQGSLIMALKEQSKLLLQKCSIIESTSLSEDAKRLQLSRDIRPQLSNMSIRIDSLEKEII KAKKDGMSKDQSKGRSQVSSQDDNIISSILPSPLEYNTSSRNSNLTSTTATTVTKALAIT GAKQNITNNTGKNSNNDSNNDDLIQVLDDEDDIDCDPPVILKEGAPHSPAFPHLHMTSEE QDELTRRRNMRSREPVNYRIPDRDDPFDYVMGKSLRDDYPDVEREEDELTMEAEDDAHSS YMTTRDEEKEENELLNQSDFDFVVNDDLDPTQDTDYHDNMDVSANIQESSQEGDTRSTIT LSQNKNVQVILSSPTAQSVPSNGQNQIGVEHIDLLEDDLEKDAILDDSMSFSFGRQHMPM SHSDLELIDSEKENEDFEEDNNNNGIEYLSDSDLERFDEERENRTQVADIQELDNDLKII TERKLTGDNEHPPPSWSPKIKREKSSVSQKDEEDDFDDDFSLSDIVSKSNLSSKTNGPTY PWSDEVLYRLHEVFKLPGFRPNQLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGK THGTTIVISPLISLMQDQVEHLLNKNIKASMFSSRGTAEQRRQTFNLFINGLLDLVYISP EMISASEQCKRAISRLYADGKLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMI ALTATASEQVRMDIIHNLELKEPVFLKQSFNRTNLYYEVNKKTKNTIFEICDAVKSRFKN QTGIIYCHSKKSCEQTSAQMQRNGIKCAYYHAGMEPDERLSVQKAWQADEIQVICATVAF GMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDK NLDRENKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKNCDNCRNSANVIN EERDVTEPAKKIVKLVESIQNERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQK SEIERIFFHLITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSR PSTSSSFQANEDNIPVIAQKSTTIGGNVAANPPRFISAKEHLRSYTYGGSTMGSSHPITL KNTSDLRSTQELNNLRMTYERLRELSLNLGNRMVPPVGNFMPDSILKKMAAILPMNDSAF ATLGTVEDKYRRRFKYFKATIADLSKKRSSEDHEKYDTILNDEFVNRAAASSNGIAQSTG TKSKFFGANLNEAKENEQIINQIRQSQLPKNTTSSKSGTRSISKSSKKSANGRRGFRNYR GHYRGRK

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Reviewed, UniProtKB/Swiss-Prot P35187 (SGS1_YEAST)

Last modified June 16, 2009. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    ATP-dependent helicase SGS1
    EC=3.6.1.-
Alternative name(s):
    Helicase TPS1

Gene names

Name:	SGS1
Synonyms:	TPS1
Ordered Locus Names:	YMR190C
ORF Names:	YM9646.02C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Interacts with topoisomerases II and TOP3. Could create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings. Together with topoisomerase II has a role in chromosomal segregation. Maintains rDNA structure where it has a role in re-starting stalled replication forks. Ref.1 Ref.2 Ref.6
Subunit structure	Heterodimer with TOP3. Forms a complex with TOP3 and RMI1. Forms a ternary complex with a MLH1-MLH3 heterodimer (MutLbeta) during meiosis. Interacts with TOP2. Ref.1 Ref.2 Ref.5 Ref.7 Ref.8
Subcellular location	Nucleus › nucleolus.
Sequence similarities	Belongs to the helicase family. RecQ subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HRDC domain.

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Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	DNA unwinding during replication Inferred from direct assay. Source: SGD double-strand break repair via homologous recombination Inferred from genetic interaction. Source: SGD gene conversion at mating-type locus, DNA double-strand break processing Inferred from genetic interaction. Source: SGD intra-S DNA damage checkpoint Inferred from genetic interaction. Source: SGD meiotic chromosome segregation Ref.2 Inferred from mutant phenotype. Source: SGD mitotic sister chromatid segregation Ref.2 Inferred from mutant phenotype. Source: SGD negative regulation of meiotic joint molecule formation Inferred from genetic interaction. Source: SGD replicative cell aging Inferred from mutant phenotype. Source: SGD telomere maintenance via recombination Inferred from mutant phenotype. Source: SGD
Cellular component	RecQ helicase-Topo III complex Ref.7 Ref.8 Inferred from direct assay. Source: SGD nucleolus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent 3'-5' DNA helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
MLH3	Q12083	1	EBI-17059,EBI-31634
SMT3	Q12306	1	EBI-17059,EBI-17490
TOP3	P13099	1	EBI-17059,EBI-19365

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1447

1447

ATP-dependent helicase SGS1

PRO_0000205057

Regions

Domain

687 – 864

178

Helicase ATP-binding

Domain

886 – 1035

150

Helicase C-terminal

Domain

1272 – 1351

HRDC

Nucleotide binding

714 – 721

ATP By similarity

Motif

808 – 811

DEAH box

Compositional bias

631 – 639

Asp/Glu-rich (highly acidic)

Experimental info

Mutagenesis

Q → P in allele sgs1-34; temperature-sensitive. Ref.6

Mutagenesis

980

T → I in allele sgs1-35; temperature-sensitive. Ref.6

Mutagenesis

987

E → K in allele sgs1-36; temperature-sensitive. Ref.6

Secondary structure

1447

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P35187-1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 0DC320B41756A3C3
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FASTA

1,447

163,837

        10         20         30         40         50         60 
MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT PSKDECGPGT 

        70         80         90        100        110        120 
TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN QYADVPMVDI PASTSVVSNP 

       130        140        150        160        170        180 
RTPNGSKTHN FNTFRPHMAS SLVENDSSRN LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR 

       190        200        210        220        230        240 
LQGSLIMALK EQSKLLLQKC SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII 

       250        260        270        280        290        300 
KAKKDGMSKD QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT 

       310        320        330        340        350        360 
GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA FPHLHMTSEE 

       370        380        390        400        410        420 
QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP DVEREEDELT MEAEDDAHSS 

       430        440        450        460        470        480 
YMTTRDEEKE ENELLNQSDF DFVVNDDLDP TQDTDYHDNM DVSANIQESS QEGDTRSTIT 

       490        500        510        520        530        540 
LSQNKNVQVI LSSPTAQSVP SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM 

       550        560        570        580        590        600 
SHSDLELIDS EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII 

       610        620        630        640        650        660 
TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN LSSKTNGPTY 

       670        680        690        700        710        720 
PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM PTGGGKSLCY QLPAVVKSGK 

       730        740        750        760        770        780 
THGTTIVISP LISLMQDQVE HLLNKNIKAS MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP 

       790        800        810        820        830        840 
EMISASEQCK RAISRLYADG KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI 

       850        860        870        880        890        900 
ALTATASEQV RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN 

       910        920        930        940        950        960 
QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE IQVICATVAF 

       970        980        990       1000       1010       1020 
GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC ITYFSFRDIR TMQTMIQKDK 

      1030       1040       1050       1060       1070       1080 
NLDRENKEKH LNKLQQVMAY CDNVTDCRRK LVLSYFNEDF DSKLCHKNCD NCRNSANVIN 

      1090       1100       1110       1120       1130       1140 
EERDVTEPAK KIVKLVESIQ NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK 

      1150       1160       1170       1180       1190       1200 
SEIERIFFHL ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR 

      1210       1220       1230       1240       1250       1260 
PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS TMGSSHPITL 

      1270       1280       1290       1300       1310       1320 
KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF 

      1330       1340       1350       1360       1370       1380 
ATLGTVEDKY RRRFKYFKAT IADLSKKRSS EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG 

      1390       1400       1410       1420       1430       1440 
TKSKFFGANL NEAKENEQII NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR 


GHYRGRK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase." Gangloff S., McDonald J.P., Bendixen C., Arthur L., Rothstein R. Mol. Cell. Biol. 14:8391-8398(1994) [PubMed: 7969174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: ATCC 200060 / W303.
[2]	"Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation." Watt P.M., Louis E.J., Borts R.H., Hickson I.D. Cell 81:253-260(1995) [PubMed: 7736577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
[3]	Romeo A.M., Kleff S., Sternglanz R. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells." Wang T.-F., Kung W.M. Biochem. Biophys. Res. Commun. 296:949-953(2002) [PubMed: 12200140] [Abstract] Cited for: INTERACTION WITH MLH3 AND TOP3.
[6]	"Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae." Kaliraman V., Brill S.J. Curr. Genet. 41:389-400(2002) [PubMed: 12228808] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLN-31; THR-980 AND GLU-987.
[7]	"RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex." Chang M., Bellaoui M., Zhang C., Desai R., Morozov P., Delgado-Cruzata L., Rothstein R., Freyer G.A., Boone C., Brown G.W. EMBO J. 24:2024-2033(2005) [PubMed: 15889139] [Abstract] Cited for: SUBUNIT.
[8]	"Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex." Mullen J.R., Nallaseth F.S., Lan Y.Q., Slagle C.E., Brill S.J. Mol. Cell. Biol. 25:4476-4487(2005) [PubMed: 15899853] [Abstract] Cited for: SUBUNIT.
[9]	"The three-dimensional structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins." Liu Z., Macias M.J., Bottomley M.J., Stier G., Linge J.P., Nilges M., Bork P., Sattler M. Structure 7:1557-1566(1999) [PubMed: 10647186] [Abstract] Cited for: STRUCTURE BY NMR OF HRDC DOMAIN.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U22341 Genomic DNA. Translation: AAB60289.1.
L07870 Genomic DNA. Translation: AAA35167.1.
Z47815 Genomic DNA. Translation: CAA87811.1.

PIR

S50918.

RefSeq

NP_013915.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D8B	NMR	-	A	1271-1351	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2911N.

IntAct

P35187. 8 interactions.

Proteomic databases

PeptideAtlas

P35187.

Genome annotation databases

Ensembl

YMR190C. Saccharomyces cerevisiae. [Contig view]

GeneID

855228.

GenomeReviews

Gene locus YMR190C in contig Z71257_GR.

KEGG

sce:YMR190C.

NMPDR

fig|4932.3.peg.4967.

Organism-specific databases

CYGD

YMR190c.

SGD

S000004802. SGS1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P35187.

OMA

P35187. TIFEICD.

Gene expression databases

ArrayExpress

P35187.

GermOnline

YMR190C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR018329. DNA_helicase_ATP-dep_RecQ_N.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR002121. HRDC.
IPR015906. HRDC-like_sub1.
IPR018982. RQC_domain.
[Graphical view]

Gene3D

G3DSA:1.10.150.80. HRDC-like_sub1. 1 hit.

PANTHER

PTHR13710. RecQ. 1 hit.

Pfam

PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00614. recQ_fam. 1 hit.

PROSITE

PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

978762.

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Entry information

Entry name

SGS1_YEAST

Accession

Primary (citable) accession number: P35187

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents