P35187 (SGS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent helicase SGS1
EC=3.6.4.-

Alternative name(s):
Helicase TPS1

Gene names

Name:	SGS1
Synonyms:	TPS1
Ordered Locus Names:	YMR190C
ORF Names:	YM9646.02C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1447 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Interacts with topoisomerases II and TOP3. Could create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings. Together with topoisomerase II has a role in chromosomal segregation. Maintains rDNA structure where it has a role in re-starting stalled replication forks. Ref.1 Ref.2 Ref.7
Subunit structure	Heterodimer with TOP3. Forms a complex with TOP3 and RMI1. Forms a ternary complex with a MLH1-MLH3 heterodimer (MutLbeta) during meiosis. Interacts with TOP2. Ref.1 Ref.2 Ref.6 Ref.8 Ref.9
Subcellular location	Nucleus › nucleolus.
Sequence similarities	Belongs to the helicase family. RecQ subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HRDC domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA unwinding involved in replication Inferred from direct assay. Source: SGD double-strand break repair via homologous recombination Inferred from mutant phenotype. Source: SGD gene conversion at mating-type locus, DNA double-strand break processing Inferred from genetic interaction. Source: SGD intra-S DNA damage checkpoint Inferred from mutant phenotype. Source: SGD meiotic chromosome segregation Inferred from mutant phenotype Ref.2. Source: SGD mitotic sister chromatid segregation Inferred from mutant phenotype Ref.2. Source: SGD negative regulation of meiotic joint molecule formation Inferred from genetic interaction. Source: SGD replicative cell aging Inferred from mutant phenotype. Source: SGD telomere maintenance via recombination Inferred from mutant phenotype. Source: SGD telomeric 3' overhang formation Inferred from genetic interaction. Source: SGD
Cellular component	RecQ helicase-Topo III complex Inferred from direct assay Ref.9. Source: SGD nucleolus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent 3'-5' DNA helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMT3	Q12306	3	EBI-17059,EBI-17490

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1447

1447

ATP-dependent helicase SGS1

PRO_0000205057

Regions

Domain

687 – 864

178

Helicase ATP-binding

Domain

886 – 1035

150

Helicase C-terminal

Domain

1272 – 1351

80

HRDC

Nucleotide binding

714 – 721

8

ATP By similarity

Motif

808 – 811

4

DEAH box

Compositional bias

631 – 639

9

Asp/Glu-rich (highly acidic)

Experimental info

Mutagenesis

31

1

Q → P in allele sgs1-34; temperature-sensitive. Ref.7

Mutagenesis

980

1

T → I in allele sgs1-35; temperature-sensitive. Ref.7

Mutagenesis

987

1

E → K in allele sgs1-36; temperature-sensitive. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1447

Helix Strand Turn

Details...

Helix

1274 – 1291

18

Beta strand

1292 – 1295

4

Helix

1303 – 1312

10

Helix

1317 – 1320

4

Helix

1321 – 1323

3

Helix

1328 – 1333

6

Helix

1334 – 1336

3

Helix

1338 – 1347

10

Turn

1348 – 1350

3

Sequences

	Sequence		Length	Mass (Da)
	P35187 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 0DC320B41756A3C3	FASTA	1,447	163,837
	10 20 30 40 50 60 MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT PSKDECGPGT 70 80 90 100 110 120 TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN QYADVPMVDI PASTSVVSNP 130 140 150 160 170 180 RTPNGSKTHN FNTFRPHMAS SLVENDSSRN LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR 190 200 210 220 230 240 LQGSLIMALK EQSKLLLQKC SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII 250 260 270 280 290 300 KAKKDGMSKD QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT 310 320 330 340 350 360 GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA FPHLHMTSEE 370 380 390 400 410 420 QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP DVEREEDELT MEAEDDAHSS 430 440 450 460 470 480 YMTTRDEEKE ENELLNQSDF DFVVNDDLDP TQDTDYHDNM DVSANIQESS QEGDTRSTIT 490 500 510 520 530 540 LSQNKNVQVI LSSPTAQSVP SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM 550 560 570 580 590 600 SHSDLELIDS EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII 610 620 630 640 650 660 TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN LSSKTNGPTY 670 680 690 700 710 720 PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM PTGGGKSLCY QLPAVVKSGK 730 740 750 760 770 780 THGTTIVISP LISLMQDQVE HLLNKNIKAS MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP 790 800 810 820 830 840 EMISASEQCK RAISRLYADG KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI 850 860 870 880 890 900 ALTATASEQV RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN 910 920 930 940 950 960 QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE IQVICATVAF 970 980 990 1000 1010 1020 GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC ITYFSFRDIR TMQTMIQKDK 1030 1040 1050 1060 1070 1080 NLDRENKEKH LNKLQQVMAY CDNVTDCRRK LVLSYFNEDF DSKLCHKNCD NCRNSANVIN 1090 1100 1110 1120 1130 1140 EERDVTEPAK KIVKLVESIQ NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK 1150 1160 1170 1180 1190 1200 SEIERIFFHL ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR 1210 1220 1230 1240 1250 1260 PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS TMGSSHPITL 1270 1280 1290 1300 1310 1320 KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF MPDSILKKMA AILPMNDSAF 1330 1340 1350 1360 1370 1380 ATLGTVEDKY RRRFKYFKAT IADLSKKRSS EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG 1390 1400 1410 1420 1430 1440 TKSKFFGANL NEAKENEQII NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase." Gangloff S., McDonald J.P., Bendixen C., Arthur L., Rothstein R. Mol. Cell. Biol. 14:8391-8398(1994) [PubMed: 7969174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT. Strain: ATCC 200060 / W303.
[2]	"Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation." Watt P.M., Louis E.J., Borts R.H., Hickson I.D. Cell 81:253-260(1995) [PubMed: 7736577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT.
[3]	Romeo A.M., Kleff S., Sternglanz R. Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells." Wang T.-F., Kung W.M. Biochem. Biophys. Res. Commun. 296:949-953(2002) [PubMed: 12200140] [Abstract] Cited for: INTERACTION WITH MLH3 AND TOP3.
[7]	"Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae." Kaliraman V., Brill S.J. Curr. Genet. 41:389-400(2002) [PubMed: 12228808] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLN-31; THR-980 AND GLU-987.
[8]	"RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex." Chang M., Bellaoui M., Zhang C., Desai R., Morozov P., Delgado-Cruzata L., Rothstein R., Freyer G.A., Boone C., Brown G.W. EMBO J. 24:2024-2033(2005) [PubMed: 15889139] [Abstract] Cited for: SUBUNIT.
[9]	"Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex." Mullen J.R., Nallaseth F.S., Lan Y.Q., Slagle C.E., Brill S.J. Mol. Cell. Biol. 25:4476-4487(2005) [PubMed: 15899853] [Abstract] Cited for: SUBUNIT.
[10]	"The three-dimensional structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins." Liu Z., Macias M.J., Bottomley M.J., Stier G., Linge J.P., Nilges M., Bork P., Sattler M. Structure 7:1557-1566(1999) [PubMed: 10647186] [Abstract] Cited for: STRUCTURE BY NMR OF HRDC DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U22341 Genomic DNA. Translation: AAB60289.1.
L07870 Genomic DNA. Translation: AAA35167.1.
Z47815 Genomic DNA. Translation: CAA87811.1.
BK006946 Genomic DNA. Translation: DAA10088.1.

PIR

S50918.

RefSeq

NP_013915.1. NM_001182696.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1D8B	NMR	-	A	1271-1351	[»]

ProteinModelPortal

P35187.

SMR

P35187. Positions 653-1193, 1271-1351.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2911N.

IntAct

P35187. 9 interactions.

MINT

MINT-442647.

STRING

P35187.

Proteomic databases

PeptideAtlas

P35187.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR190C; YMR190C; YMR190C.

GeneID

855228.

KEGG

sce:YMR190C.

NMPDR

fig|4932.3.peg.4967.

Organism-specific databases

CYGD

YMR190c.

SGD

S000004802. SGS1.

Phylogenomic databases

eggNOG

fuNOG05215.

GeneTree

EFGT00050000000108.

HOGENOM

HBG203478.

OMA

TIFEICD.

OrthoDB

EOG4XSPZ5.

Gene expression databases

ArrayExpress

P35187.

Genevestigator

P35187.

GermOnline

YMR190C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR002121. Helicase/RNaseD_C.
IPR001650. Helicase_C.
IPR022758. Helicase_Sgs1.
IPR010997. HRDC-like.
IPR015906. HRDC-like_sub1.
IPR018982. RQC_domain.
[Graphical view]

Gene3D

G3DSA:1.10.150.80. HRDC-like_sub1. 1 hit.

KO

K10901.

PANTHER

PTHR13710. RecQ. 1 hit.

Pfam

PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF11408. Helicase_Sgs1. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]

SMART

SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]

SUPFAM

SSF47819. HRDC_like. 1 hit.

TIGRFAMs

TIGR00614. RecQ_fam. 1 hit.

PROSITE

PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

978762.

Entry information

Entry name

SGS1_YEAST

Accession

Primary (citable) accession number: P35187
Secondary accession number(s): D6W014

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 25, 2012
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program