UniProtKB - P35187 (SGS1_YEAST)
(max 400 entries)x
Your basket is currently empty. i
Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)
Protein
ATP-dependent helicase SGS1
Gene
SGS1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
ATP-dependent DNA helicase able to unwind duplex DNA or DNA- RNA heteroduplex (PubMed:9545297). Displacement of the DNA strand occurs in the 3' to 5' direction with respect to the single-stranded DNA flanking the duplex (PubMed:9545297). Acts as an integral component of the S-phase checkpoint response, which arrests cells due to DNA damage or blocked fork progression during DNA replication (PubMed:10640278). Can create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings (PubMed:7969174). Together with topoisomerase II has a role in chromosomal segregation (PubMed:7736577). Maintains rDNA structure where it has a role in re-starting stalled replication forks (PubMed:12228808).5 Publications
Catalytic activityi
ATP + H2O = ADP + phosphate.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 714 – 721 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent 3'-5' DNA helicase activity Source: GO_Central
- ATP-dependent DNA helicase activity Source: SGD
- DNA binding Source: GO_Central
- four-way junction helicase activity Source: GO_Central
GO - Biological processi
- chromosome organization Source: SGD
- DNA double-strand break processing Source: SGD
- DNA duplex unwinding Source: SGD
- DNA topological change Source: SGD
- DNA unwinding involved in DNA replication Source: SGD
- double-strand break repair via homologous recombination Source: SGD
- gene conversion at mating-type locus, DNA double-strand break processing Source: SGD
- intra-S DNA damage checkpoint Source: SGD
- meiotic chromosome segregation Source: SGD
- meiotic DNA double-strand break processing Source: SGD
- mitotic sister chromatid segregation Source: SGD
- negative regulation of meiotic joint molecule formation Source: SGD
- regulation of reciprocal meiotic recombination Source: SGD
- replicative cell aging Source: SGD
- telomere maintenance Source: SGD
- telomere maintenance via recombination Source: SGD
- telomeric 3' overhang formation Source: SGD
Keywordsi
Molecular function | Helicase, Hydrolase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | YEAST:G3O-32877-MONOMER. |
BRENDAi | 3.6.4.12. 984. |
Reactomei | R-SCE-3108214. SUMOylation of DNA damage response and repair proteins. |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent helicase SGS11 Publication (EC:3.6.4.121 Publication)Alternative name(s): Helicase TPS1 |
Gene namesi | Ordered Locus Names:YMR190CImported ORF Names:YM9646.02C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YMR190C. |
SGDi | S000004802. SGS1. |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | Q → P in allele sgs1-34; temperature-sensitive. 1 Publication | 1 | |
Mutagenesisi | 980 | T → I in allele sgs1-35; temperature-sensitive. 1 Publication | 1 | |
Mutagenesisi | 987 | E → K in allele sgs1-36; temperature-sensitive. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000205057 | 1 – 1447 | ATP-dependent helicase SGS1Add BLAST | 1447 |
Proteomic databases
MaxQBi | P35187. |
PaxDbi | P35187. |
PRIDEi | P35187. |
PTM databases
iPTMneti | P35187. |
Expressioni
Inductioni
Expression is regulated through the cell cycle with an accumulation in S phase.1 Publication
Interactioni
Subunit structurei
Heterodimer with TOP3 (PubMed:7969174, PubMed:15899853). Forms a complex with TOP3 and RMI1 (PubMed:15889139). Forms a ternary complex with a MLH1-MLH3 heterodimer (MutLbeta) during meiosis (PubMed:12200140). Interacts with TOP2 (PubMed:7736577).5 Publications
Binary interactionsi
Protein-protein interaction databases
BioGridi | 35368. 545 interactors. |
DIPi | DIP-2911N. |
IntActi | P35187. 22 interactors. |
MINTi | P35187. |
STRINGi | 4932.YMR190C. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 1274 – 1291 | Combined sources | 18 | |
Beta strandi | 1292 – 1295 | Combined sources | 4 | |
Helixi | 1303 – 1312 | Combined sources | 10 | |
Helixi | 1317 – 1320 | Combined sources | 4 | |
Helixi | 1321 – 1323 | Combined sources | 3 | |
Helixi | 1328 – 1333 | Combined sources | 6 | |
Helixi | 1334 – 1336 | Combined sources | 3 | |
Helixi | 1338 – 1347 | Combined sources | 10 | |
Turni | 1348 – 1350 | Combined sources | 3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D8B | NMR | - | A | 1271-1351 | [»] | |
ProteinModelPortali | P35187. | |||||
SMRi | P35187. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P35187. |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 687 – 864 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 886 – 1035 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 150 | |
Domaini | 1272 – 1351 | HRDCPROSITE-ProRule annotationAdd BLAST | 80 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 808 – 811 | DEAH box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 631 – 639 | Asp/Glu-rich (highly acidic) | 9 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00550000074520. |
HOGENOMi | HOG000141897. |
InParanoidi | P35187. |
KOi | K10901. |
OMAi | VTLIYCQ. |
OrthoDBi | EOG092C0HS8. |
Family and domain databases
CDDi | cd00079. HELICc. 1 hit. |
Gene3Di | 1.10.10.10. 1 hit. |
InterProi | View protein in InterPro IPR011545. DEAD/DEAH_box_helicase_dom. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR004589. DNA_helicase_ATP-dep_RecQ. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR022758. Helicase_Sgs1. IPR010997. HRDC-like_sf. IPR002121. HRDC_dom. IPR027417. P-loop_NTPase. IPR032284. RecQ_Zn-bd. IPR018982. RQC_domain. IPR036388. WH-like_DNA-bd_sf. |
Pfami | View protein in Pfam PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. PF11408. Helicase_Sgs1. 1 hit. PF16124. RecQ_Zn_bind. 1 hit. PF09382. RQC. 1 hit. |
SMARTi | View protein in SMART SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00341. HRDC. 1 hit. SM00956. RQC. 1 hit. |
SUPFAMi | SSF47819. SSF47819. 1 hit. SSF52540. SSF52540. 3 hits. |
TIGRFAMsi | TIGR00614. recQ_fam. 1 hit. |
PROSITEi | View protein in PROSITE PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50967. HRDC. 1 hit. |
i Sequence
Sequence statusi: Complete.
P35187-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT
60 70 80 90 100
PSKDECGPGT TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN
110 120 130 140 150
QYADVPMVDI PASTSVVSNP RTPNGSKTHN FNTFRPHMAS SLVENDSSRN
160 170 180 190 200
LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR LQGSLIMALK EQSKLLLQKC
210 220 230 240 250
SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII KAKKDGMSKD
260 270 280 290 300
QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT
310 320 330 340 350
GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA
360 370 380 390 400
FPHLHMTSEE QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP
410 420 430 440 450
DVEREEDELT MEAEDDAHSS YMTTRDEEKE ENELLNQSDF DFVVNDDLDP
460 470 480 490 500
TQDTDYHDNM DVSANIQESS QEGDTRSTIT LSQNKNVQVI LSSPTAQSVP
510 520 530 540 550
SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM SHSDLELIDS
560 570 580 590 600
EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII
610 620 630 640 650
TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN
660 670 680 690 700
LSSKTNGPTY PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM
710 720 730 740 750
PTGGGKSLCY QLPAVVKSGK THGTTIVISP LISLMQDQVE HLLNKNIKAS
760 770 780 790 800
MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP EMISASEQCK RAISRLYADG
810 820 830 840 850
KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI ALTATASEQV
860 870 880 890 900
RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN
910 920 930 940 950
QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE
960 970 980 990 1000
IQVICATVAF GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC
1010 1020 1030 1040 1050
ITYFSFRDIR TMQTMIQKDK NLDRENKEKH LNKLQQVMAY CDNVTDCRRK
1060 1070 1080 1090 1100
LVLSYFNEDF DSKLCHKNCD NCRNSANVIN EERDVTEPAK KIVKLVESIQ
1110 1120 1130 1140 1150
NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK SEIERIFFHL
1160 1170 1180 1190 1200
ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR
1210 1220 1230 1240 1250
PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS
1260 1270 1280 1290 1300
TMGSSHPITL KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF
1310 1320 1330 1340 1350
MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS
1360 1370 1380 1390 1400
EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG TKSKFFGANL NEAKENEQII
1410 1420 1430 1440
NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U22341 Genomic DNA. Translation: AAB60289.1. L07870 Genomic DNA. Translation: AAA35167.1. Z47815 Genomic DNA. Translation: CAA87811.1. BK006946 Genomic DNA. Translation: DAA10088.1. |
PIRi | S50918. |
RefSeqi | NP_013915.1. NM_001182696.1. |
Genome annotation databases
EnsemblFungii | YMR190C; YMR190C; YMR190C. |
GeneIDi | 855228. |
KEGGi | sce:YMR190C. |
Similar proteinsi
Entry informationi
Entry namei | SGS1_YEAST | |
Accessioni | P35187Primary (citable) accession number: P35187 Secondary accession number(s): D6W014 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1994 |
Last sequence update: | February 1, 1994 | |
Last modified: | March 28, 2018 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |