P35183 (AST1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein AST1

Gene names

Name:	AST1
Ordered Locus Names:	YBL069W
ORF Names:	YBL0617, YBL06.04

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reroutes mutant ATPase from the vacuole to the cell surface. Participates in membrane traffic. Interacts with PMA1.
Subcellular location	Membrane; Peripheral membrane protein.

Ontologies

Keywords
Cellular component	Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein targeting to membrane Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	extrinsic to membrane Traceable author statement. Source: SGD
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Protein AST1		PRO_0000064710
Experimental info
	Sequence conflict	326 – 429	104	TVGDY…NVEKF → HCR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35183 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 9F2126C2230AE20D	FASTA	429	48,349
	10 20 30 40 50 60 MAKDILKNQD PKLQAMIVEH SAPAPKEIPM DAPVLKRVAR PLRHVKFIPI KSLIFHTKTG 70 80 90 100 110 120 PMDFSYEKKI KTPIPKNKIV VRVSNVGLNP VDMKIRNGYT SSIYGEIGLG REYSGVITEV 130 140 150 160 170 180 GENLNYAWHV GDEVYGIYYH PHLAVGCLQS SILVDPKVDP ILLRPESVSA EEAAGSLFCL 190 200 210 220 230 240 ATGYNILNKL SKNKYLKQDS NVLINGGTSS VGMFVIQLLK RHYKLQKKLV IVTSANGPQV 250 260 270 280 290 300 LQEKFPDLAD EMIFIDYLTC RGKSSKPLRK MLEEKKISQY DPVEDKETIL NYNEGKFDVV 310 320 330 340 350 360 LDFVGGYDIL SHSSSLIHGG GAYVTTVGDY VANYKEDIFD SWDNPSANAR KMFGSIIWSY 370 380 390 400 410 420 NYTHYYFDPN AKTASANNDW IEQCGDFLKN GTVKCVVDKV YDWKDHKEAF SYMATQRAQG KLIMNVEKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1 prevents mislocalization of mutant ATPase to the vacuole." Chang A., Fink G.R. J. Cell Biol. 128:39-49(1995) [PubMed: 7822420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4." Logghe M., Molemans F., Fiers W., Contreras R. Yeast 10:1093-1100(1994) [PubMed: 7992509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X81843 Genomic DNA. Translation: CAA57435.1. Z26879 Genomic DNA. Translation: CAA81522.1. Z35831 Genomic DNA. Translation: CAA84891.1. Z35829 Genomic DNA. Translation: CAA84889.1. BK006936 Genomic DNA. Translation: DAA07052.1.
PIR	S45588.
RefSeq	NP_009484.2. NM_001178309.1.
3D structure databases
ProteinModelPortal	P35183.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35183. 2 interactions.
STRING	P35183.
Proteomic databases
PeptideAtlas	P35183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL069W; YBL069W; YBL069W.
GeneID	852209.
KEGG	sce:YBL069W.
NMPDR	fig|4932.3.peg.172.
Organism-specific databases
CYGD	YBL069w.
SGD	S000000165. AST1.
Phylogenomic databases
eggNOG	fuNOG04078.
GeneTree	EFGT00050000001805.
HOGENOM	HBG204241.
OMA	LGKGTCE.
OrthoDB	EOG45TGX5.
Gene expression databases
ArrayExpress	P35183.
Genevestigator	P35183.
GermOnline	YBL069W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
ProtoNet	Search...
Other
NextBio	970712.

Entry information

Entry name

AST1_YEAST

Accession

Primary (citable) accession number: P35183
Secondary accession number(s): D6VPT2, P89492

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program