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Protein

Protein phosphatase 2C homolog 1

Gene

PTC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

It has a serine and a weak tyrosine phosphatase activity with ratios of serine to tyrosine phosphatase activity as high as 200:1. It is essential for growth or germination at 37 degrees Celsius. May have a role in the heat shock response. Involved in tRNA splicing and cell separation.

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+, Mn2+Note: Binds 2 magnesium or manganese ions per subunit. Manganese is about 20 times more efficient than magnesium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Manganese 1By similarity1
Metal bindingi58Manganese 2By similarity1
Metal bindingi59Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi233Manganese 2By similarity1
Metal bindingi272Manganese 2By similarity1

GO - Molecular functioni

  • MAP kinase serine/threonine phosphatase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  • inactivation of MAPK activity Source: SGD
  • inactivation of MAPKK activity Source: SGD
  • mitochondrion inheritance Source: SGD
  • negative regulation of MAPK cascade Source: SGD
  • peptidyl-threonine dephosphorylation Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • protein dephosphorylation Source: SGD
  • tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processStress response
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29437-MONOMER
ReactomeiR-SCE-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C homolog 1 (EC:3.1.3.16)
Short name:
PP2C-1
Gene namesi
Name:PTC1
Synonyms:TPD1
Ordered Locus Names:YDL006W
ORF Names:D2925
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL006W
SGDiS000002164 PTC1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577741 – 281Protein phosphatase 2C homolog 1Add BLAST281

Proteomic databases

MaxQBiP35182
PaxDbiP35182
PRIDEiP35182

PTM databases

iPTMnetiP35182

Interactioni

Subunit structurei

Interacts with NBP2 and PBS2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP2Q121634EBI-12784,EBI-34713

Protein-protein interaction databases

BioGridi32048, 804 interactors
DIPiDIP-1537N
IntActiP35182, 13 interactors
MINTiP35182
STRINGi4932.YDL006W

Structurei

3D structure databases

ProteinModelPortaliP35182
SMRiP35182
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 281PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST262

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140907
HOGENOMiHOG000233896
InParanoidiP35182
KOiK19704
OMAiVMSGRVN
OrthoDBiEOG092C52JO

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P35182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD
60 70 80 90 100
WGYFAVFDGH AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI
110 120 130 140 150
DEEINTKLVG NSGCTAAVCV LRWELPDSVS DDSMDLAQHQ RKLYTANVGD
160 170 180 190 200
SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE QAGGLIMKSR VNGMLAVTRS
210 220 230 240 250
LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID DQDACELIKD
260 270 280
ITEPNEAAKV LVRYALENGT TDNVTVMVVF L
Length:281
Mass (Da):31,550
Last modified:February 1, 1994 - v1
Checksum:iFA384D2541B20D50
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53Y → G (PubMed:8196609).Curated1
Sequence conflicti124E → Q (PubMed:8196609).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14593 Genomic DNA Translation: AAA34920.1
Z48432 Genomic DNA Translation: CAA88353.1
Z74054 Genomic DNA Translation: CAA98562.1
Z48008 Genomic DNA Translation: CAA88055.1
BK006938 Genomic DNA Translation: DAA11842.1
PIRiS41854
RefSeqiNP_010278.3, NM_001180065.3

Genome annotation databases

EnsemblFungiiYDL006W; YDL006W; YDL006W
GeneIDi851558
KEGGisce:YDL006W

Similar proteinsi

Entry informationi

Entry nameiPP2C1_YEAST
AccessioniPrimary (citable) accession number: P35182
Secondary accession number(s): D6VRY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health