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P35182 (PP2C1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C homolog 1

Short name=PP2C-1
EC=3.1.3.16
Gene names
Name:PTC1
Synonyms:TPD1
Ordered Locus Names:YDL006W
ORF Names:D2925
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length281 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

It has a serine and a weak tyrosine phosphatase activity with ratios of serine to tyrosine phosphatase activity as high as 200:1. It is essential for growth or germination at 37 degrees Celsius. May have a role in the heat shock response. Involved in tRNA splicing and cell separation.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit. Manganese is about 20 times more efficient than magnesium.

Subunit structure

Interacts with NBP2 and PBS2. Ref.6

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PP2C family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NBP2Q121634EBI-12784,EBI-34713

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 281281Protein phosphatase 2C homolog 1
PRO_0000057774

Sites

Metal binding581Manganese 1 By similarity
Metal binding581Manganese 2 By similarity
Metal binding591Manganese 1; via carbonyl oxygen By similarity
Metal binding2331Manganese 2 By similarity
Metal binding2721Manganese 2 By similarity

Experimental info

Sequence conflict531Y → G Ref.2
Sequence conflict1241E → Q Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35182 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: FA384D2541B20D50

FASTA28131,550
        10         20         30         40         50         60 
MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD WGYFAVFDGH 

        70         80         90        100        110        120 
AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI DEEINTKLVG NSGCTAAVCV 

       130        140        150        160        170        180 
LRWELPDSVS DDSMDLAQHQ RKLYTANVGD SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE 

       190        200        210        220        230        240 
QAGGLIMKSR VNGMLAVTRS LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID 

       250        260        270        280 
DQDACELIKD ITEPNEAAKV LVRYALENGT TDNVTVMVVF L 

« Hide

References

« Hide 'large scale' references
[1]"Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae."
Maeda T., Tsai A.Y.M., Saito H.
Mol. Cell. Biol. 13:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation."
Robinson M.K., van Zyl W.H., Phizicky E.M., Broach J.R.
Mol. Cell. Biol. 14:3634-3645(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway."
Mapes J., Ota I.M.
EMBO J. 23:302-311(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NBP2 AND PBS2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L14593 Genomic DNA. Translation: AAA34920.1.
Z48432 Genomic DNA. Translation: CAA88353.1.
Z74054 Genomic DNA. Translation: CAA98562.1.
Z48008 Genomic DNA. Translation: CAA88055.1.
BK006938 Genomic DNA. Translation: DAA11842.1.
PIRS41854.
RefSeqNP_010278.3. NM_001180065.3.

3D structure databases

ProteinModelPortalP35182.
SMRP35182. Positions 31-279.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32048. 406 interactions.
DIPDIP-1537N.
IntActP35182. 6 interactions.
MINTMINT-392615.
STRING4932.YDL006W.

Proteomic databases

MaxQBP35182.
PaxDbP35182.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL006W; YDL006W; YDL006W.
GeneID851558.
KEGGsce:YDL006W.

Organism-specific databases

CYGDYDL006w.
SGDS000002164. PTC1.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000114971.
HOGENOMHOG000233896.
KOK01090.
OMAGHAGKAT.
OrthoDBEOG7D2FR2.

Enzyme and pathway databases

BioCycYEAST:G3O-29437-MONOMER.

Gene expression databases

GenevestigatorP35182.

Family and domain databases

Gene3D3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968986.
PROP35182.

Entry information

Entry namePP2C1_YEAST
AccessionPrimary (citable) accession number: P35182
Secondary accession number(s): D6VRY2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families