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P35182

- PP2C1_YEAST

UniProt

P35182 - PP2C1_YEAST

Protein

Protein phosphatase 2C homolog 1

Gene

PTC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    It has a serine and a weak tyrosine phosphatase activity with ratios of serine to tyrosine phosphatase activity as high as 200:1. It is essential for growth or germination at 37 degrees Celsius. May have a role in the heat shock response. Involved in tRNA splicing and cell separation.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit. Manganese is about 20 times more efficient than magnesium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi58 – 581Manganese 1By similarity
    Metal bindingi58 – 581Manganese 2By similarity
    Metal bindingi59 – 591Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi233 – 2331Manganese 2By similarity
    Metal bindingi272 – 2721Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
    2. mitochondrion inheritance Source: SGD
    3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    4. protein dephosphorylation Source: SGD
    5. response to stress Source: UniProtKB-KW
    6. tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29437-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C homolog 1 (EC:3.1.3.16)
    Short name:
    PP2C-1
    Gene namesi
    Name:PTC1
    Synonyms:TPD1
    Ordered Locus Names:YDL006W
    ORF Names:D2925
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL006w.
    SGDiS000002164. PTC1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Protein phosphatase 2C homolog 1PRO_0000057774Add
    BLAST

    Proteomic databases

    MaxQBiP35182.
    PaxDbiP35182.

    Expressioni

    Gene expression databases

    GenevestigatoriP35182.

    Interactioni

    Subunit structurei

    Interacts with NBP2 and PBS2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NBP2Q121634EBI-12784,EBI-34713

    Protein-protein interaction databases

    BioGridi32048. 407 interactions.
    DIPiDIP-1537N.
    IntActiP35182. 6 interactions.
    MINTiMINT-392615.
    STRINGi4932.YDL006W.

    Structurei

    3D structure databases

    ProteinModelPortaliP35182.
    SMRiP35182. Positions 31-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PP2C family.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    GeneTreeiENSGT00740000114971.
    HOGENOMiHOG000233896.
    KOiK01090.
    OMAiGHAGKAT.
    OrthoDBiEOG7D2FR2.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.
    PROSITEiPS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35182-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD    50
    WGYFAVFDGH AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI 100
    DEEINTKLVG NSGCTAAVCV LRWELPDSVS DDSMDLAQHQ RKLYTANVGD 150
    SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE QAGGLIMKSR VNGMLAVTRS 200
    LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID DQDACELIKD 250
    ITEPNEAAKV LVRYALENGT TDNVTVMVVF L 281
    Length:281
    Mass (Da):31,550
    Last modified:February 1, 1994 - v1
    Checksum:iFA384D2541B20D50
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531Y → G(PubMed:8196609)Curated
    Sequence conflicti124 – 1241E → Q(PubMed:8196609)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14593 Genomic DNA. Translation: AAA34920.1.
    Z48432 Genomic DNA. Translation: CAA88353.1.
    Z74054 Genomic DNA. Translation: CAA98562.1.
    Z48008 Genomic DNA. Translation: CAA88055.1.
    BK006938 Genomic DNA. Translation: DAA11842.1.
    PIRiS41854.
    RefSeqiNP_010278.3. NM_001180065.3.

    Genome annotation databases

    EnsemblFungiiYDL006W; YDL006W; YDL006W.
    GeneIDi851558.
    KEGGisce:YDL006W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L14593 Genomic DNA. Translation: AAA34920.1 .
    Z48432 Genomic DNA. Translation: CAA88353.1 .
    Z74054 Genomic DNA. Translation: CAA98562.1 .
    Z48008 Genomic DNA. Translation: CAA88055.1 .
    BK006938 Genomic DNA. Translation: DAA11842.1 .
    PIRi S41854.
    RefSeqi NP_010278.3. NM_001180065.3.

    3D structure databases

    ProteinModelPortali P35182.
    SMRi P35182. Positions 31-279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32048. 407 interactions.
    DIPi DIP-1537N.
    IntActi P35182. 6 interactions.
    MINTi MINT-392615.
    STRINGi 4932.YDL006W.

    Proteomic databases

    MaxQBi P35182.
    PaxDbi P35182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL006W ; YDL006W ; YDL006W .
    GeneIDi 851558.
    KEGGi sce:YDL006W.

    Organism-specific databases

    CYGDi YDL006w.
    SGDi S000002164. PTC1.

    Phylogenomic databases

    eggNOGi COG0631.
    GeneTreei ENSGT00740000114971.
    HOGENOMi HOG000233896.
    KOi K01090.
    OMAi GHAGKAT.
    OrthoDBi EOG7D2FR2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29437-MONOMER.

    Miscellaneous databases

    NextBioi 968986.
    PROi P35182.

    Gene expression databases

    Genevestigatori P35182.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    PROSITEi PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae."
      Maeda T., Tsai A.Y.M., Saito H.
      Mol. Cell. Biol. 13:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation."
      Robinson M.K., van Zyl W.H., Phizicky E.M., Broach J.R.
      Mol. Cell. Biol. 14:3634-3645(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway."
      Mapes J., Ota I.M.
      EMBO J. 23:302-311(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NBP2 AND PBS2.

    Entry informationi

    Entry nameiPP2C1_YEAST
    AccessioniPrimary (citable) accession number: P35182
    Secondary accession number(s): D6VRY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1520 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3