Reviewed, UniProtKB/Swiss-Prot P35182 (PP2C1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 2C homolog 1
      Short name=PP2C-1
    EC=3.1.3.16

Gene names

Name:	PTC1
Synonyms:	TPD1
Ordered Locus Names:	YDL006W
ORF Names:	D2925

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	It has a serine and a weak tyrosine phosphatase activity with ratios of serine to tyrosine phosphatase activity as high as 200:1. It is essential for growth or germination at 37 degrees Celsius. May have a role in the heat shock response. Involved in tRNA splicing and cell separation.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit. Manganese is about 20 times more efficient than magnesium.
Subunit structure	Interacts with NBP2 and PBS2. Ref.5
Miscellaneous	Present with 1520 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Biological process	Stress response
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inactivation of MAPK activity involved in osmosensory signaling pathway Inferred from direct assay. Source: SGD mitochondrion inheritance Inferred from mutant phenotype. Source: SGD protein amino acid dephosphorylation Inferred from direct assay. Source: SGD tRNA splicing, via endonucleolytic cleavage and ligation Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine phosphatase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARE2	P53629	1	EBI-12784,EBI-2808
NBP2	Q12163	4	EBI-12784,EBI-34713
TSL1	P38427	1	EBI-12784,EBI-19638

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Protein phosphatase 2C homolog 1		PRO_0000057774
Sites
	Metal binding	58	1	Manganese 1 By similarity
	Metal binding	58	1	Manganese 2 By similarity
	Metal binding	59	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	233	1	Manganese 2 By similarity
	Metal binding	272	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	53	1	Y → G Ref.2
	Sequence conflict	124	1	E → Q Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35182-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: FA384D2541B20D50	FASTA	281	31,550
	10 20 30 40 50 60 MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD WGYFAVFDGH 70 80 90 100 110 120 AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI DEEINTKLVG NSGCTAAVCV 130 140 150 160 170 180 LRWELPDSVS DDSMDLAQHQ RKLYTANVGD SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE 190 200 210 220 230 240 QAGGLIMKSR VNGMLAVTRS LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID 250 260 270 280 DQDACELIKD ITEPNEAAKV LVRYALENGT TDNVTVMVVF L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae." Maeda T., Tsai A.Y.M., Saito H. Mol. Cell. Biol. 13:5408-5417(1993) [PubMed: 8395005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation." Robinson M.K., van Zyl W.H., Phizicky E.M., Broach J.R. Mol. Cell. Biol. 14:3634-3645(1994) [PubMed: 8196609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway." Mapes J., Ota I.M. EMBO J. 23:302-311(2004) [PubMed: 14685261] [Abstract] Cited for: INTERACTION WITH NBP2 AND PBS2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L14593 Genomic DNA. Translation: AAA34920.1. Z48432 Genomic DNA. Translation: CAA88353.1. Z74054 Genomic DNA. Translation: CAA98562.1. Z48008 Genomic DNA. Translation: CAA88055.1.
PIR	S41854.
RefSeq	NP_010278.1.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1537N.
IntAct	P35182. 10 interactions.
STRING	P35182.
Genome annotation databases
Ensembl	YDL006W; YDL006W; YDL006W; Saccharomyces cerevisiae. [Genome view]
GeneID	851558.
GenomeReviews	Gene locus YDL006W in contig Z71256_GR.
KEGG	sce:YDL006W.
NMPDR	fig|4932.3.peg.1020.
Organism-specific databases
CYGD	YDL006w.
SGD	S000002164. PTC1.
Phylogenomic databases
HOGENOM	P35182.
OMA	FTTSVEI.
Enzyme and pathway databases
BRENDA	3.1.3.16. 250.
Gene expression databases
ArrayExpress	P35182.
Genevestigator	P35182.
GermOnline	YDL006W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015655. PP2C. IPR001932. PP2C-related. IPR000222. PP2C_Mn2_Asp60_BS. IPR014045. PP2C_N. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 1 hit.
PANTHER	PTHR13832. PP2C. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968986.

Entry information

Entry name

PP2C1_YEAST

Accession

Primary (citable) accession number: P35182

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)