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P35182

- PP2C1_YEAST

UniProt

P35182 - PP2C1_YEAST

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Protein

Protein phosphatase 2C homolog 1

Gene

PTC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

It has a serine and a weak tyrosine phosphatase activity with ratios of serine to tyrosine phosphatase activity as high as 200:1. It is essential for growth or germination at 37 degrees Celsius. May have a role in the heat shock response. Involved in tRNA splicing and cell separation.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit. Manganese is about 20 times more efficient than magnesium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Manganese 1By similarity
Metal bindingi58 – 581Manganese 2By similarity
Metal bindingi59 – 591Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi233 – 2331Manganese 2By similarity
Metal bindingi272 – 2721Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  1. inactivation of MAPK activity involved in osmosensory signaling pathway Source: SGD
  2. mitochondrion inheritance Source: SGD
  3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  4. protein dephosphorylation Source: SGD
  5. response to stress Source: UniProtKB-KW
  6. tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29437-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C homolog 1 (EC:3.1.3.16)
Short name:
PP2C-1
Gene namesi
Name:PTC1
Synonyms:TPD1
Ordered Locus Names:YDL006W
ORF Names:D2925
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL006w.
SGDiS000002164. PTC1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Protein phosphatase 2C homolog 1PRO_0000057774Add
BLAST

Proteomic databases

MaxQBiP35182.
PaxDbiP35182.

Expressioni

Gene expression databases

GenevestigatoriP35182.

Interactioni

Subunit structurei

Interacts with NBP2 and PBS2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NBP2Q121634EBI-12784,EBI-34713

Protein-protein interaction databases

BioGridi32048. 410 interactions.
DIPiDIP-1537N.
IntActiP35182. 6 interactions.
MINTiMINT-392615.
STRINGi4932.YDL006W.

Structurei

3D structure databases

ProteinModelPortaliP35182.
SMRiP35182. Positions 31-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000114971.
HOGENOMiHOG000233896.
InParanoidiP35182.
KOiK01090.
OMAiGHAGKAT.
OrthoDBiEOG7D2FR2.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35182-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNHSEILER PETPYDITYR VGVAENKNSK FRRTMEDVHT YVKNFASRLD
60 70 80 90 100
WGYFAVFDGH AGIQASKWCG KHLHTIIEQN ILADETRDVR DVLNDSFLAI
110 120 130 140 150
DEEINTKLVG NSGCTAAVCV LRWELPDSVS DDSMDLAQHQ RKLYTANVGD
160 170 180 190 200
SRIVLFRNGN SIRLTYDHKA SDTLEMQRVE QAGGLIMKSR VNGMLAVTRS
210 220 230 240 250
LGDKFFDSLV VGSPFTTSVE ITSEDKFLIL ACDGLWDVID DQDACELIKD
260 270 280
ITEPNEAAKV LVRYALENGT TDNVTVMVVF L
Length:281
Mass (Da):31,550
Last modified:February 1, 1994 - v1
Checksum:iFA384D2541B20D50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531Y → G(PubMed:8196609)Curated
Sequence conflicti124 – 1241E → Q(PubMed:8196609)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14593 Genomic DNA. Translation: AAA34920.1.
Z48432 Genomic DNA. Translation: CAA88353.1.
Z74054 Genomic DNA. Translation: CAA98562.1.
Z48008 Genomic DNA. Translation: CAA88055.1.
BK006938 Genomic DNA. Translation: DAA11842.1.
PIRiS41854.
RefSeqiNP_010278.3. NM_001180065.3.

Genome annotation databases

EnsemblFungiiYDL006W; YDL006W; YDL006W.
GeneIDi851558.
KEGGisce:YDL006W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L14593 Genomic DNA. Translation: AAA34920.1 .
Z48432 Genomic DNA. Translation: CAA88353.1 .
Z74054 Genomic DNA. Translation: CAA98562.1 .
Z48008 Genomic DNA. Translation: CAA88055.1 .
BK006938 Genomic DNA. Translation: DAA11842.1 .
PIRi S41854.
RefSeqi NP_010278.3. NM_001180065.3.

3D structure databases

ProteinModelPortali P35182.
SMRi P35182. Positions 31-279.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32048. 410 interactions.
DIPi DIP-1537N.
IntActi P35182. 6 interactions.
MINTi MINT-392615.
STRINGi 4932.YDL006W.

Proteomic databases

MaxQBi P35182.
PaxDbi P35182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL006W ; YDL006W ; YDL006W .
GeneIDi 851558.
KEGGi sce:YDL006W.

Organism-specific databases

CYGDi YDL006w.
SGDi S000002164. PTC1.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000114971.
HOGENOMi HOG000233896.
InParanoidi P35182.
KOi K01090.
OMAi GHAGKAT.
OrthoDBi EOG7D2FR2.

Enzyme and pathway databases

BioCyci YEAST:G3O-29437-MONOMER.

Miscellaneous databases

NextBioi 968986.
PROi P35182.

Gene expression databases

Genevestigatori P35182.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations in a protein tyrosine phosphatase gene (PTP2) and a protein serine/threonine phosphatase gene (PTC1) cause a synthetic growth defect in Saccharomyces cerevisiae."
    Maeda T., Tsai A.Y.M., Saito H.
    Mol. Cell. Biol. 13:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation."
    Robinson M.K., van Zyl W.H., Phizicky E.M., Broach J.R.
    Mol. Cell. Biol. 14:3634-3645(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway."
    Mapes J., Ota I.M.
    EMBO J. 23:302-311(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NBP2 AND PBS2.

Entry informationi

Entry nameiPP2C1_YEAST
AccessioniPrimary (citable) accession number: P35182
Secondary accession number(s): D6VRY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3