P35181 (AP1S1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit sigma-1

Alternative name(s):
Clathrin assembly protein complex 1 small chain
Clathrin coat assembly protein AP19
Clathrin coat-associated protein AP19
Golgi adaptor AP-1 19 kDa adaptin
HA1 19 kDa subunit
Sigma1-adaptin

Gene names

Name:	APS1
Synonyms:	YAP19
Ordered Locus Names:	YLR170C
ORF Names:	L9470.16

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP19 is probably a subunit of the Golgi membrane adaptor. Ref.6
Subunit structure	Assembly protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin. Ref.6
Subcellular location	Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › clathrin-coated pit. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Miscellaneous	Present with 6370 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the adaptor complexes small subunit family.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Golgi apparatus Membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to vacuole transport Inferred from mutant phenotype PubMed 17003107. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular_component	AP-1 adaptor complex Inferred from physical interaction Ref.6. Source: SGD coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	clathrin binding Inferred from sequence or structural similarity Ref.6. Source: SGD protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APL2	P36000	5	EBI-2612,EBI-2206
APL4	Q12028	6	EBI-2612,EBI-33025
APM1	Q00776	4	EBI-2612,EBI-2624

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 156	156	AP-1 complex subunit sigma-1		PRO_0000193803

Sequences

	Sequence		Length	Mass (Da)
	P35181 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 1A584883F1DF815D	FASTA	156	18,152
	10 20 30 40 50 60 MTQLKYLLLV SRQGKIRLKK WYTAMSAGEK AKIVKDLTPT ILARKPKMCN IIEYNDHKVV 70 80 90 100 110 120 YKRYASLYFI VGMTPDVDNE LLTLEIIHRF VETMDTYFGN VCELDIIFNF SKVYDILNEM 130 140 150 IMCDGSIAES SRKEVLHHVT VMDTMESNDN LERVLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins." Nakai M., Takada T., Endo T. Biochim. Biophys. Acta 1174:282-284(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP1.
[2]	"The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex." Phan H.L., Finlay J.A., Chu D.S., Tan P.K., Kirchhausen T., Payne G.S. EMBO J. 13:1706-1717(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GPY 297.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70279 Genomic DNA. Translation: CAA49765.1. Z30314 Genomic DNA. Translation: CAA82959.2. U17246 Genomic DNA. Translation: AAB67468.1. AY692851 Genomic DNA. Translation: AAT92870.1. BK006945 Genomic DNA. Translation: DAA09491.1.
PIR	S37757.
RefSeq	NP_013271.1. NM_001182057.1.
3D structure databases
ProteinModelPortal	P35181.
SMR	P35181. Positions 4-149.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4806N.
IntAct	P35181. 8 interactions.
MINT	MINT-475400.
STRING	4932.YLR170C.
Proteomic databases
PaxDb	P35181.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR170C; YLR170C; YLR170C.
GeneID	850868.
KEGG	sce:YLR170C.
Organism-specific databases
CYGD	YLR170c.
SGD	S000004160. APS1.
Phylogenomic databases
eggNOG	COG5030.
GeneTree	ENSGT00530000062839.
HOGENOM	HOG000185227.
OMA	MMQFMLL.
OrthoDB	EOG45MRFT.
Gene expression databases
Genevestigator	P35181.
GermOnline	YLR170C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016635. AP_complex_ssu. IPR022775. AP_mu_sigma_su. IPR000804. Clathrin_sm-chain_CS. IPR011012. Longin-like_dom. [Graphical view]
PANTHER	PTHR11753. PTHR11753. 1 hit.
Pfam	PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF015588. AP_complex_sigma. 1 hit.
SUPFAM	SSF64356. Longin_like. 1 hit.
PROSITE	PS00989. CLAT_ADAPTOR_S. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967197.

Entry information

Entry name

AP1S1_YEAST

Accession

Primary (citable) accession number: P35181
Secondary accession number(s): D6VYH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program