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P35181

- AP1S1_YEAST

UniProt

P35181 - AP1S1_YEAST

Protein

AP-1 complex subunit sigma-1

Gene

APS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP19 is probably a subunit of the Golgi membrane adaptor.1 Publication

    GO - Molecular functioni

    1. clathrin binding Source: SGD
    2. protein binding Source: IntAct
    3. protein transporter activity Source: InterPro

    GO - Biological processi

    1. Golgi to vacuole transport Source: SGD
    2. intracellular protein transport Source: InterPro
    3. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32299-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit sigma-1
    Alternative name(s):
    Clathrin assembly protein complex 1 sigma-1 small chain
    Clathrin coat assembly protein AP19
    Clathrin coat-associated protein AP19
    Golgi adaptor AP-1 19 kDa adaptin
    HA1 19 kDa subunit
    Sigma1-adaptin
    Gene namesi
    Name:APS1
    Synonyms:YAP19
    Ordered Locus Names:YLR170C
    ORF Names:L9470.16
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR170c.
    SGDiS000004160. APS1.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. AP-1 adaptor complex Source: SGD
    2. coated pit Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 156156AP-1 complex subunit sigma-1PRO_0000193803Add
    BLAST

    Proteomic databases

    MaxQBiP35181.
    PaxDbiP35181.

    Expressioni

    Gene expression databases

    GenevestigatoriP35181.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APL2P360005EBI-2612,EBI-2206
    APL4Q120286EBI-2612,EBI-33025
    APM1Q007764EBI-2612,EBI-2624

    Protein-protein interaction databases

    BioGridi31442. 82 interactions.
    DIPiDIP-4806N.
    IntActiP35181. 8 interactions.
    MINTiMINT-475400.
    STRINGi4932.YLR170C.

    Structurei

    3D structure databases

    ProteinModelPortaliP35181.
    SMRiP35181. Positions 4-149.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG5030.
    GeneTreeiENSGT00530000062839.
    HOGENOMiHOG000185227.
    OMAiVERAKMG.
    OrthoDBiEOG79KPTF.

    Family and domain databases

    InterProiIPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view]
    PANTHERiPTHR11753. PTHR11753. 1 hit.
    PfamiPF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMiSSF64356. SSF64356. 1 hit.
    PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35181-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQLKYLLLV SRQGKIRLKK WYTAMSAGEK AKIVKDLTPT ILARKPKMCN    50
    IIEYNDHKVV YKRYASLYFI VGMTPDVDNE LLTLEIIHRF VETMDTYFGN 100
    VCELDIIFNF SKVYDILNEM IMCDGSIAES SRKEVLHHVT VMDTMESNDN 150
    LERVLS 156
    Length:156
    Mass (Da):18,152
    Last modified:February 1, 1994 - v1
    Checksum:i1A584883F1DF815D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70279 Genomic DNA. Translation: CAA49765.1.
    Z30314 Genomic DNA. Translation: CAA82959.2.
    U17246 Genomic DNA. Translation: AAB67468.1.
    AY692851 Genomic DNA. Translation: AAT92870.1.
    BK006945 Genomic DNA. Translation: DAA09491.1.
    PIRiS37757.
    RefSeqiNP_013271.1. NM_001182057.1.

    Genome annotation databases

    EnsemblFungiiYLR170C; YLR170C; YLR170C.
    GeneIDi850868.
    KEGGisce:YLR170C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70279 Genomic DNA. Translation: CAA49765.1 .
    Z30314 Genomic DNA. Translation: CAA82959.2 .
    U17246 Genomic DNA. Translation: AAB67468.1 .
    AY692851 Genomic DNA. Translation: AAT92870.1 .
    BK006945 Genomic DNA. Translation: DAA09491.1 .
    PIRi S37757.
    RefSeqi NP_013271.1. NM_001182057.1.

    3D structure databases

    ProteinModelPortali P35181.
    SMRi P35181. Positions 4-149.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31442. 82 interactions.
    DIPi DIP-4806N.
    IntActi P35181. 8 interactions.
    MINTi MINT-475400.
    STRINGi 4932.YLR170C.

    Proteomic databases

    MaxQBi P35181.
    PaxDbi P35181.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR170C ; YLR170C ; YLR170C .
    GeneIDi 850868.
    KEGGi sce:YLR170C.

    Organism-specific databases

    CYGDi YLR170c.
    SGDi S000004160. APS1.

    Phylogenomic databases

    eggNOGi COG5030.
    GeneTreei ENSGT00530000062839.
    HOGENOMi HOG000185227.
    OMAi VERAKMG.
    OrthoDBi EOG79KPTF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32299-MONOMER.

    Miscellaneous databases

    NextBioi 967197.

    Gene expression databases

    Genevestigatori P35181.

    Family and domain databases

    InterProi IPR016635. AP_complex_ssu.
    IPR022775. AP_mu_sigma_su.
    IPR000804. Clathrin_sm-chain_CS.
    IPR011012. Longin-like_dom.
    [Graphical view ]
    PANTHERi PTHR11753. PTHR11753. 1 hit.
    Pfami PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
    SUPFAMi SSF64356. SSF64356. 1 hit.
    PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins."
      Nakai M., Takada T., Endo T.
      Biochim. Biophys. Acta 1174:282-284(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SP1.
    2. "The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex."
      Phan H.L., Finlay J.A., Chu D.S., Tan P.K., Kirchhausen T., Payne G.S.
      EMBO J. 13:1706-1717(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GPY 297.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Adaptor complex-independent clathrin function in yeast."
      Yeung B.G., Phan H.L., Payne G.S.
      Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP1S1_YEAST
    AccessioniPrimary (citable) accession number: P35181
    Secondary accession number(s): D6VYH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6370 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3