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P35181 (AP1S1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit sigma-1
Alternative name(s):
Clathrin assembly protein complex 1 sigma-1 small chain
Clathrin coat assembly protein AP19
Clathrin coat-associated protein AP19
Golgi adaptor AP-1 19 kDa adaptin
HA1 19 kDa subunit
Sigma1-adaptin
Gene names
Name:APS1
Synonyms:YAP19
Ordered Locus Names:YLR170C
ORF Names:L9470.16
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length156 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP19 is probably a subunit of the Golgi membrane adaptor. Ref.6

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin. Ref.6

Subcellular location

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Miscellaneous

Present with 6370 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the adaptor complexes small subunit family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 156156AP-1 complex subunit sigma-1
PRO_0000193803

Sequences

Sequence LengthMass (Da)Tools
P35181 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 1A584883F1DF815D

FASTA15618,152
        10         20         30         40         50         60 
MTQLKYLLLV SRQGKIRLKK WYTAMSAGEK AKIVKDLTPT ILARKPKMCN IIEYNDHKVV 

        70         80         90        100        110        120 
YKRYASLYFI VGMTPDVDNE LLTLEIIHRF VETMDTYFGN VCELDIIFNF SKVYDILNEM 

       130        140        150 
IMCDGSIAES SRKEVLHHVT VMDTMESNDN LERVLS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins."
Nakai M., Takada T., Endo T.
Biochim. Biophys. Acta 1174:282-284(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SP1.
[2]"The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex."
Phan H.L., Finlay J.A., Chu D.S., Tan P.K., Kirchhausen T., Payne G.S.
EMBO J. 13:1706-1717(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GPY 297.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Adaptor complex-independent clathrin function in yeast."
Yeung B.G., Phan H.L., Payne G.S.
Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70279 Genomic DNA. Translation: CAA49765.1.
Z30314 Genomic DNA. Translation: CAA82959.2.
U17246 Genomic DNA. Translation: AAB67468.1.
AY692851 Genomic DNA. Translation: AAT92870.1.
BK006945 Genomic DNA. Translation: DAA09491.1.
PIRS37757.
RefSeqNP_013271.1. NM_001182057.1.

3D structure databases

ProteinModelPortalP35181.
SMRP35181. Positions 4-149.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31442. 82 interactions.
DIPDIP-4806N.
IntActP35181. 8 interactions.
MINTMINT-475400.
STRING4932.YLR170C.

Proteomic databases

PaxDbP35181.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR170C; YLR170C; YLR170C.
GeneID850868.
KEGGsce:YLR170C.

Organism-specific databases

CYGDYLR170c.
SGDS000004160. APS1.

Phylogenomic databases

eggNOGCOG5030.
GeneTreeENSGT00530000062839.
HOGENOMHOG000185227.
OMAFGSICEL.
OrthoDBEOG79KPTF.

Enzyme and pathway databases

BioCycYEAST:G3O-32299-MONOMER.

Gene expression databases

GenevestigatorP35181.

Family and domain databases

InterProIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERPTHR11753. PTHR11753. 1 hit.
PfamPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMSSF64356. SSF64356. 1 hit.
PROSITEPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967197.

Entry information

Entry nameAP1S1_YEAST
AccessionPrimary (citable) accession number: P35181
Secondary accession number(s): D6VYH5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families