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P35181

- AP1S1_YEAST

UniProt

P35181 - AP1S1_YEAST

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Protein

AP-1 complex subunit sigma-1

Gene

APS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP19 is probably a subunit of the Golgi membrane adaptor.1 Publication

GO - Molecular functioni

  1. clathrin binding Source: SGD
  2. protein transporter activity Source: InterPro

GO - Biological processi

  1. Golgi to vacuole transport Source: SGD
  2. intracellular protein transport Source: InterPro
  3. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32299-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit sigma-1
Alternative name(s):
Clathrin assembly protein complex 1 sigma-1 small chain
Clathrin coat assembly protein AP19
Clathrin coat-associated protein AP19
Golgi adaptor AP-1 19 kDa adaptin
HA1 19 kDa subunit
Sigma1-adaptin
Gene namesi
Name:APS1
Synonyms:YAP19
Ordered Locus Names:YLR170C
ORF Names:L9470.16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR170c.
SGDiS000004160. APS1.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membraneclathrin-coated pit
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. AP-1 adaptor complex Source: SGD
  2. coated pit Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 156156AP-1 complex subunit sigma-1PRO_0000193803Add
BLAST

Proteomic databases

MaxQBiP35181.
PaxDbiP35181.

Expressioni

Gene expression databases

GenevestigatoriP35181.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). AP-1 interacts with clathrin.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
APL2P360005EBI-2612,EBI-2206
APL4Q120286EBI-2612,EBI-33025
APM1Q007764EBI-2612,EBI-2624

Protein-protein interaction databases

BioGridi31442. 82 interactions.
DIPiDIP-4806N.
IntActiP35181. 8 interactions.
MINTiMINT-475400.
STRINGi4932.YLR170C.

Structurei

3D structure databases

ProteinModelPortaliP35181.
SMRiP35181. Positions 4-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5030.
GeneTreeiENSGT00530000062839.
HOGENOMiHOG000185227.
InParanoidiP35181.
OMAiVERAKMG.
OrthoDBiEOG79KPTF.

Family and domain databases

InterProiIPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view]
PANTHERiPTHR11753. PTHR11753. 1 hit.
PfamiPF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMiSSF64356. SSF64356. 1 hit.
PROSITEiPS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35181-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQLKYLLLV SRQGKIRLKK WYTAMSAGEK AKIVKDLTPT ILARKPKMCN
60 70 80 90 100
IIEYNDHKVV YKRYASLYFI VGMTPDVDNE LLTLEIIHRF VETMDTYFGN
110 120 130 140 150
VCELDIIFNF SKVYDILNEM IMCDGSIAES SRKEVLHHVT VMDTMESNDN

LERVLS
Length:156
Mass (Da):18,152
Last modified:February 1, 1994 - v1
Checksum:i1A584883F1DF815D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70279 Genomic DNA. Translation: CAA49765.1.
Z30314 Genomic DNA. Translation: CAA82959.2.
U17246 Genomic DNA. Translation: AAB67468.1.
AY692851 Genomic DNA. Translation: AAT92870.1.
BK006945 Genomic DNA. Translation: DAA09491.1.
PIRiS37757.
RefSeqiNP_013271.1. NM_001182057.1.

Genome annotation databases

EnsemblFungiiYLR170C; YLR170C; YLR170C.
GeneIDi850868.
KEGGisce:YLR170C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70279 Genomic DNA. Translation: CAA49765.1 .
Z30314 Genomic DNA. Translation: CAA82959.2 .
U17246 Genomic DNA. Translation: AAB67468.1 .
AY692851 Genomic DNA. Translation: AAT92870.1 .
BK006945 Genomic DNA. Translation: DAA09491.1 .
PIRi S37757.
RefSeqi NP_013271.1. NM_001182057.1.

3D structure databases

ProteinModelPortali P35181.
SMRi P35181. Positions 4-149.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31442. 82 interactions.
DIPi DIP-4806N.
IntActi P35181. 8 interactions.
MINTi MINT-475400.
STRINGi 4932.YLR170C.

Proteomic databases

MaxQBi P35181.
PaxDbi P35181.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR170C ; YLR170C ; YLR170C .
GeneIDi 850868.
KEGGi sce:YLR170C.

Organism-specific databases

CYGDi YLR170c.
SGDi S000004160. APS1.

Phylogenomic databases

eggNOGi COG5030.
GeneTreei ENSGT00530000062839.
HOGENOMi HOG000185227.
InParanoidi P35181.
OMAi VERAKMG.
OrthoDBi EOG79KPTF.

Enzyme and pathway databases

BioCyci YEAST:G3O-32299-MONOMER.

Miscellaneous databases

NextBioi 967197.

Gene expression databases

Genevestigatori P35181.

Family and domain databases

InterProi IPR016635. AP_complex_ssu.
IPR022775. AP_mu_sigma_su.
IPR000804. Clathrin_sm-chain_CS.
IPR011012. Longin-like_dom.
[Graphical view ]
PANTHERi PTHR11753. PTHR11753. 1 hit.
Pfami PF01217. Clat_adaptor_s. 1 hit.
[Graphical view ]
PIRSFi PIRSF015588. AP_complex_sigma. 1 hit.
SUPFAMi SSF64356. SSF64356. 1 hit.
PROSITEi PS00989. CLAT_ADAPTOR_S. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the YAP19 gene encoding a putative yeast homolog of AP19, the mammalian small chain of the clathrin-assembly proteins."
    Nakai M., Takada T., Endo T.
    Biochim. Biophys. Acta 1174:282-284(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SP1.
  2. "The Saccharomyces cerevisiae APS1 gene encodes a homolog of the small subunit of the mammalian clathrin AP-1 complex: evidence for functional interaction with clathrin at the Golgi complex."
    Phan H.L., Finlay J.A., Chu D.S., Tan P.K., Kirchhausen T., Payne G.S.
    EMBO J. 13:1706-1717(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GPY 297.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Adaptor complex-independent clathrin function in yeast."
    Yeung B.G., Phan H.L., Payne G.S.
    Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH CLATHRIN.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP1S1_YEAST
AccessioniPrimary (citable) accession number: P35181
Secondary accession number(s): D6VYH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6370 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3