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P35180 (TOM20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import receptor subunit TOM20
Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Protein MAS20
Translocase of outer membrane 20 kDa subunit
Gene names
Name:TOM20
Synonyms:MAS20, MOM19
Ordered Locus Names:YGR082W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length183 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

Subunit structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex, interacts with TOM22. Ref.6 Ref.7

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein Potential.

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Tom20 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TOM22P493344EBI-12522,EBI-12527
TOM40P236443EBI-12522,EBI-12539

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 183183Mitochondrial import receptor subunit TOM20
PRO_0000051550

Regions

Topological domain1 – 88Mitochondrial intermembrane Potential
Transmembrane9 – 2820Helical; Potential
Topological domain29 – 183155Cytoplasmic Potential

Amino acid modifications

Modified residue1721Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P35180 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9F76FE6010278410

FASTA18320,317
        10         20         30         40         50         60 
MSQSNPILRG LAITTAIAAL SATGYAIYFD YQRRNSPQFR KVLRQRAKEQ AKMEEQAKTH 

        70         80         90        100        110        120 
AKEVKLQKVT EFLSMELAKD PIPSDPSERE ATFTTNVENG ERLSMQQGKE LEAASKFYKA 

       130        140        150        160        170        180 
LTVYPQPADL LGIYQRSIPE AIYEYIILMI AILPPANVAS FVKGVVGSKA ESDAVAEAND 


IDD 

« Hide

References

« Hide 'large scale' references
[1]"Functional cooperation of mitochondrial protein import receptors in yeast."
Ramage L., Junne T., Hahne K., Lithgow T., Schatz G.
EMBO J. 12:4115-4123(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria."
Moczko M., Ehmann B., Gaertner F., Hoenlinger A., Schaefer E., Pfanner N.
J. Biol. Chem. 269:9045-9051(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TOM COMPLEX.
[7]"Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase."
van Wilpe S., Ryan M.T., Hill K., Maarse A.C., Meisinger C., Brix J., Dekker P.J.T., Moczko M., Wagner R., Meijer M., Guiard B., Hoenlinger A., Pfanner N.
Nature 401:485-489(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TOM22.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76139 Genomic DNA. Translation: CAA53742.1.
X75319 mRNA. Translation: CAA53068.1.
Z72867 Genomic DNA. Translation: CAA97084.1.
AY557775 Genomic DNA. Translation: AAS56101.1.
BK006941 Genomic DNA. Translation: DAA08175.1.
PIRS39568.
RefSeqNP_011596.1. NM_001181211.1.

3D structure databases

ProteinModelPortalP35180.
SMRP35180. Positions 76-151.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33324. 27 interactions.
DIPDIP-2539N.
IntActP35180. 14 interactions.
MINTMINT-513344.
STRING4932.YGR082W.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

PaxDbP35180.
PeptideAtlasP35180.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR082W; YGR082W; YGR082W.
GeneID852973.
KEGGsce:YGR082W.

Organism-specific databases

CYGDYGR082w.
SGDS000003314. TOM20.

Phylogenomic databases

eggNOGNOG298500.
HOGENOMHOG000216577.
KOK17770.
OMAAFFNEQV.
OrthoDBEOG7W6WZ8.

Enzyme and pathway databases

BioCycYEAST:G3O-30794-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

GenevestigatorP35180.

Family and domain databases

Gene3D1.20.960.10. 1 hit.
InterProIPR002056. MAS20_rcpt-related.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERPTHR12430. PTHR12430. 1 hit.
PfamPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSPR00351. OM20RECEPTOR.
SUPFAMSSF47157. SSF47157. 1 hit.
TIGRFAMsTIGR00985. 3a0801s04tom. 1 hit.
ProtoNetSearch...

Other

NextBio972765.
PROP35180.

Entry information

Entry nameTOM20_YEAST
AccessionPrimary (citable) accession number: P35180
Secondary accession number(s): D6VUL4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families