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P35180

- TOM20_YEAST

UniProt

P35180 - TOM20_YEAST

Protein

Mitochondrial import receptor subunit TOM20

Gene

TOM20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

    GO - Molecular functioni

    1. mitochondrion targeting sequence binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitochondrial outer membrane translocase complex assembly Source: SGD
    2. protein import into mitochondrial matrix Source: SGD
    3. tRNA import into mitochondrion Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30794-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import receptor subunit TOM20
    Alternative name(s):
    Mitochondrial 20 kDa outer membrane protein
    Protein MAS20
    Translocase of outer membrane 20 kDa subunit
    Gene namesi
    Name:TOM20
    Synonyms:MAS20, MOM19
    Ordered Locus Names:YGR082W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR082w.
    SGDiS000003314. TOM20.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. mitochondrial outer membrane Source: Reactome
    3. mitochondrial outer membrane translocase complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Mitochondrial import receptor subunit TOM20PRO_0000051550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei172 – 1721Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP35180.
    PaxDbiP35180.
    PeptideAtlasiP35180.

    Expressioni

    Gene expression databases

    GenevestigatoriP35180.

    Interactioni

    Subunit structurei

    Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex, interacts with TOM22.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TOM22P493344EBI-12522,EBI-12527
    TOM40P236443EBI-12522,EBI-12539

    Protein-protein interaction databases

    BioGridi33324. 27 interactions.
    DIPiDIP-2539N.
    IntActiP35180. 14 interactions.
    MINTiMINT-513344.
    STRINGi4932.YGR082W.

    Structurei

    3D structure databases

    ProteinModelPortaliP35180.
    SMRiP35180. Positions 76-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88Mitochondrial intermembraneSequence Analysis
    Topological domaini29 – 183155CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2820HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Tom20 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG298500.
    HOGENOMiHOG000216577.
    KOiK17770.
    OMAiAFFNEQV.
    OrthoDBiEOG7W6WZ8.

    Family and domain databases

    Gene3Di1.20.960.10. 1 hit.
    InterProiIPR002056. MAS20_rcpt-related.
    IPR023392. Tom20_dom.
    [Graphical view]
    PANTHERiPTHR12430. PTHR12430. 1 hit.
    PfamiPF02064. MAS20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
    PRINTSiPR00351. OM20RECEPTOR.
    SUPFAMiSSF47157. SSF47157. 1 hit.
    TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P35180-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQSNPILRG LAITTAIAAL SATGYAIYFD YQRRNSPQFR KVLRQRAKEQ    50
    AKMEEQAKTH AKEVKLQKVT EFLSMELAKD PIPSDPSERE ATFTTNVENG 100
    ERLSMQQGKE LEAASKFYKA LTVYPQPADL LGIYQRSIPE AIYEYIILMI 150
    AILPPANVAS FVKGVVGSKA ESDAVAEAND IDD 183
    Length:183
    Mass (Da):20,317
    Last modified:February 1, 1994 - v1
    Checksum:i9F76FE6010278410
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76139 Genomic DNA. Translation: CAA53742.1.
    X75319 mRNA. Translation: CAA53068.1.
    Z72867 Genomic DNA. Translation: CAA97084.1.
    AY557775 Genomic DNA. Translation: AAS56101.1.
    BK006941 Genomic DNA. Translation: DAA08175.1.
    PIRiS39568.
    RefSeqiNP_011596.1. NM_001181211.1.

    Genome annotation databases

    EnsemblFungiiYGR082W; YGR082W; YGR082W.
    GeneIDi852973.
    KEGGisce:YGR082W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76139 Genomic DNA. Translation: CAA53742.1 .
    X75319 mRNA. Translation: CAA53068.1 .
    Z72867 Genomic DNA. Translation: CAA97084.1 .
    AY557775 Genomic DNA. Translation: AAS56101.1 .
    BK006941 Genomic DNA. Translation: DAA08175.1 .
    PIRi S39568.
    RefSeqi NP_011596.1. NM_001181211.1.

    3D structure databases

    ProteinModelPortali P35180.
    SMRi P35180. Positions 76-151.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33324. 27 interactions.
    DIPi DIP-2539N.
    IntActi P35180. 14 interactions.
    MINTi MINT-513344.
    STRINGi 4932.YGR082W.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P35180.
    PaxDbi P35180.
    PeptideAtlasi P35180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR082W ; YGR082W ; YGR082W .
    GeneIDi 852973.
    KEGGi sce:YGR082W.

    Organism-specific databases

    CYGDi YGR082w.
    SGDi S000003314. TOM20.

    Phylogenomic databases

    eggNOGi NOG298500.
    HOGENOMi HOG000216577.
    KOi K17770.
    OMAi AFFNEQV.
    OrthoDBi EOG7W6WZ8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30794-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 972765.
    PROi P35180.

    Gene expression databases

    Genevestigatori P35180.

    Family and domain databases

    Gene3Di 1.20.960.10. 1 hit.
    InterProi IPR002056. MAS20_rcpt-related.
    IPR023392. Tom20_dom.
    [Graphical view ]
    PANTHERi PTHR12430. PTHR12430. 1 hit.
    Pfami PF02064. MAS20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037707. MAS20_rcpt. 1 hit.
    PRINTSi PR00351. OM20RECEPTOR.
    SUPFAMi SSF47157. SSF47157. 1 hit.
    TIGRFAMsi TIGR00985. 3a0801s04tom. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional cooperation of mitochondrial protein import receptors in yeast."
      Ramage L., Junne T., Hahne K., Lithgow T., Schatz G.
      EMBO J. 12:4115-4123(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria."
      Moczko M., Ehmann B., Gaertner F., Hoenlinger A., Schaefer E., Pfanner N.
      J. Biol. Chem. 269:9045-9051(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
      Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
      Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TOM COMPLEX.
    7. Cited for: INTERACTION WITH TOM22.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTOM20_YEAST
    AccessioniPrimary (citable) accession number: P35180
    Secondary accession number(s): D6VUL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5680 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3