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P35180

- TOM20_YEAST

UniProt

P35180 - TOM20_YEAST

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Protein
Mitochondrial import receptor subunit TOM20
Gene
TOM20, MAS20, MOM19, YGR082W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

GO - Molecular functioni

  1. mitochondrion targeting sequence binding Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. mitochondrial outer membrane translocase complex assembly Source: SGD
  2. protein import into mitochondrial matrix Source: SGD
  3. tRNA import into mitochondrion Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30794-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20
Alternative name(s):
Mitochondrial 20 kDa outer membrane protein
Protein MAS20
Translocase of outer membrane 20 kDa subunit
Gene namesi
Name:TOM20
Synonyms:MAS20, MOM19
Ordered Locus Names:YGR082W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR082w.
SGDiS000003314. TOM20.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88Mitochondrial intermembrane Reviewed prediction
Transmembranei9 – 2820Helical; Reviewed prediction
Add
BLAST
Topological domaini29 – 183155Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: SGD
  2. mitochondrial outer membrane Source: Reactome
  3. mitochondrial outer membrane translocase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Mitochondrial import receptor subunit TOM20
PRO_0000051550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei172 – 1721Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35180.
PaxDbiP35180.
PeptideAtlasiP35180.

Expressioni

Gene expression databases

GenevestigatoriP35180.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70). In the complex, interacts with TOM22.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TOM22P493344EBI-12522,EBI-12527
TOM40P236443EBI-12522,EBI-12539

Protein-protein interaction databases

BioGridi33324. 27 interactions.
DIPiDIP-2539N.
IntActiP35180. 14 interactions.
MINTiMINT-513344.
STRINGi4932.YGR082W.

Structurei

3D structure databases

ProteinModelPortaliP35180.
SMRiP35180. Positions 76-151.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tom20 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG298500.
HOGENOMiHOG000216577.
KOiK17770.
OMAiAFFNEQV.
OrthoDBiEOG7W6WZ8.

Family and domain databases

Gene3Di1.20.960.10. 1 hit.
InterProiIPR002056. MAS20_rcpt-related.
IPR023392. Tom20_dom.
[Graphical view]
PANTHERiPTHR12430. PTHR12430. 1 hit.
PfamiPF02064. MAS20. 1 hit.
[Graphical view]
PIRSFiPIRSF037707. MAS20_rcpt. 1 hit.
PRINTSiPR00351. OM20RECEPTOR.
SUPFAMiSSF47157. SSF47157. 1 hit.
TIGRFAMsiTIGR00985. 3a0801s04tom. 1 hit.

Sequencei

Sequence statusi: Complete.

P35180-1 [UniParc]FASTAAdd to Basket

« Hide

MSQSNPILRG LAITTAIAAL SATGYAIYFD YQRRNSPQFR KVLRQRAKEQ    50
AKMEEQAKTH AKEVKLQKVT EFLSMELAKD PIPSDPSERE ATFTTNVENG 100
ERLSMQQGKE LEAASKFYKA LTVYPQPADL LGIYQRSIPE AIYEYIILMI 150
AILPPANVAS FVKGVVGSKA ESDAVAEAND IDD 183
Length:183
Mass (Da):20,317
Last modified:February 1, 1994 - v1
Checksum:i9F76FE6010278410
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76139 Genomic DNA. Translation: CAA53742.1.
X75319 mRNA. Translation: CAA53068.1.
Z72867 Genomic DNA. Translation: CAA97084.1.
AY557775 Genomic DNA. Translation: AAS56101.1.
BK006941 Genomic DNA. Translation: DAA08175.1.
PIRiS39568.
RefSeqiNP_011596.1. NM_001181211.1.

Genome annotation databases

EnsemblFungiiYGR082W; YGR082W; YGR082W.
GeneIDi852973.
KEGGisce:YGR082W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76139 Genomic DNA. Translation: CAA53742.1 .
X75319 mRNA. Translation: CAA53068.1 .
Z72867 Genomic DNA. Translation: CAA97084.1 .
AY557775 Genomic DNA. Translation: AAS56101.1 .
BK006941 Genomic DNA. Translation: DAA08175.1 .
PIRi S39568.
RefSeqi NP_011596.1. NM_001181211.1.

3D structure databases

ProteinModelPortali P35180.
SMRi P35180. Positions 76-151.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33324. 27 interactions.
DIPi DIP-2539N.
IntActi P35180. 14 interactions.
MINTi MINT-513344.
STRINGi 4932.YGR082W.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi P35180.
PaxDbi P35180.
PeptideAtlasi P35180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR082W ; YGR082W ; YGR082W .
GeneIDi 852973.
KEGGi sce:YGR082W.

Organism-specific databases

CYGDi YGR082w.
SGDi S000003314. TOM20.

Phylogenomic databases

eggNOGi NOG298500.
HOGENOMi HOG000216577.
KOi K17770.
OMAi AFFNEQV.
OrthoDBi EOG7W6WZ8.

Enzyme and pathway databases

BioCyci YEAST:G3O-30794-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 972765.
PROi P35180.

Gene expression databases

Genevestigatori P35180.

Family and domain databases

Gene3Di 1.20.960.10. 1 hit.
InterProi IPR002056. MAS20_rcpt-related.
IPR023392. Tom20_dom.
[Graphical view ]
PANTHERi PTHR12430. PTHR12430. 1 hit.
Pfami PF02064. MAS20. 1 hit.
[Graphical view ]
PIRSFi PIRSF037707. MAS20_rcpt. 1 hit.
PRINTSi PR00351. OM20RECEPTOR.
SUPFAMi SSF47157. SSF47157. 1 hit.
TIGRFAMsi TIGR00985. 3a0801s04tom. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional cooperation of mitochondrial protein import receptors in yeast."
    Ramage L., Junne T., Hahne K., Lithgow T., Schatz G.
    EMBO J. 12:4115-4123(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria."
    Moczko M., Ehmann B., Gaertner F., Hoenlinger A., Schaefer E., Pfanner N.
    J. Biol. Chem. 269:9045-9051(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex."
    Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.
    Mol. Cell. Biol. 18:6515-6524(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TOM COMPLEX.
  7. Cited for: INTERACTION WITH TOM22.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTOM20_YEAST
AccessioniPrimary (citable) accession number: P35180
Secondary accession number(s): D6VUL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5680 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi