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Protein

Protein transport protein SSS1

Gene

SSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Sec61 complex, which is the major component of channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). The functional states of the translocon complex include co- and post-translational ER import, cotranslational membrane protein integration and retrograde transport of misfolded proteins out of the ER. In the cotranslational pathway, ribosomes synthesizing presecretory proteins are targeted to the translocon by the cytosolic signal recognition particle (SRP) and its ER-localized receptor. The association of the Sec61 complex with the ribosome is mediated by the 28S rRNA of the large ribosomal subunit. SRP-independent post-translational translocation requires the association of additional factors, such as the Sec62/63 complex and KAR2. Also part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein ER import.

GO - Molecular functioni

  • P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  • protein transporter activity Source: SGD
  • structural molecule activity Source: SGD

GO - Biological processi

  • posttranslational protein targeting to membrane, translocation Source: SGD
  • SRP-dependent cotranslational protein targeting to membrane, translocation Source: SGD

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29691-MONOMER
ReactomeiR-SCE-381038 XBP1(S) activates chaperone genes

Protein family/group databases

TCDBi3.A.5.8.1 the general secretory pathway (sec) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SSS1
Alternative name(s):
Sec61 complex subunit SSS1
Sec61 complex subunit gamma
Ssh1 complex subunit SSS1
Ssh1 complex subunit gamma
Gene namesi
Name:SSS1
Ordered Locus Names:YDR086C
ORF Names:D4475
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR086C
SGDiS000002493 SSS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 46CytoplasmicSequence analysisAdd BLAST46
Transmembranei47 – 75HelicalSequence analysisAdd BLAST29
Topological domaini76 – 80ExtracellularSequence analysis5

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001042111 – 80Protein transport protein SSS1Add BLAST80

Proteomic databases

MaxQBiP35179
PaxDbiP35179
PRIDEiP35179
TopDownProteomicsiP35179

PTM databases

iPTMnetiP35179

Interactioni

Subunit structurei

Component of the heterotrimeric Sec61 complex, which is composed of SSH1, SBH1 and SSS1. Presumably three to four Sec61 heterotrimers assemble into an oligomeric ring with a central aqueous pore. In cotranslational ER import, the pore diameter varies from 9-15 A in a ribosome-free resting state to 40-60 A in a functional state when associated with the ribosome. The Sec61 complex is part of a channel-forming translocon complex whose composition seem to change dependent upon different functional states. During post-translational ER import the Sec61 complex associates with the Sec62/63 complex to form the Sec complex. SSH1 is a component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SBH2 and SSS1. SSS1 interacts with OST1, OST4, SWP1 and WBP1, components of the OT complex.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32142290 interactors.
DIPiDIP-2432N
IntActiP35179 19 interactors.
MINTiP35179
STRINGi4932.YDR086C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WW9electron microscopy8.60B1-80[»]
2WWAelectron microscopy8.90B1-80[»]
ProteinModelPortaliP35179
SMRiP35179
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35179

Family & Domainsi

Sequence similaritiesi

Belongs to the SecE/SEC61-gamma family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000001319
HOGENOMiHOG000194479
InParanoidiP35179
KOiK07342
OMAiQFLTRCT
OrthoDBiEOG092C5W8H

Family and domain databases

Gene3Di1.20.5.8201 hit
HAMAPiMF_00422 SecE, 1 hit
InterProiView protein in InterPro
IPR023391 Prot_translocase_SecE_dom_sf
IPR022943 SecE
IPR008158 Translocase_Sec61-g
IPR001901 Translocase_SecE/Sec61-g
PfamiView protein in Pfam
PF00584 SecE, 1 hit
SUPFAMiSSF103456 SSF103456, 1 hit
TIGRFAMsiTIGR00327 secE_euk_arch, 1 hit
PROSITEiView protein in PROSITE
PS01067 SECE_SEC61G, 1 hit

Sequencei

Sequence statusi: Complete.

P35179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARASEKGEE KKQSNNQVEK LVEAPVEFVR EGTQFLAKCK KPDLKEYTKI
60 70 80
VKAVGIGFIA VGIIGYAIKL IHIPIRYVIV
Length:80
Mass (Da):8,944
Last modified:September 27, 2004 - v2
Checksum:i340F2EA913850D85
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58F → L in CAA52608 (PubMed:8223425).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46796 Genomic DNA Translation: CAA86808.1
X74499 Genomic DNA Translation: CAA52608.1
X82086 Genomic DNA Translation: CAA57615.1
Z74382 Genomic DNA Translation: CAA98906.1
AY557659 Genomic DNA Translation: AAS55985.1
BK006938 Genomic DNA Translation: DAA11933.1
PIRiS48775
RefSeqiNP_010371.1, NM_001180394.1

Genome annotation databases

EnsemblFungiiYDR086C; YDR086C; YDR086C
GeneIDi851659
KEGGisce:YDR086C

Similar proteinsi

Entry informationi

Entry nameiSC61G_YEAST
AccessioniPrimary (citable) accession number: P35179
Secondary accession number(s): D6VS73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: September 27, 2004
Last modified: March 28, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome