NTH2

Functionⁱ

Miscellaneous

Present with 2500 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

Alpha,alpha-trehalose + H₂O = beta-D-glucose + alpha-D-glucose.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	331	SubstrateBy similarity	1
Binding siteⁱ	375	SubstrateBy similarity	1
Binding siteⁱ	384	SubstrateBy similarity	1
Binding siteⁱ	505	Substrate; via carbonyl oxygenBy similarity	1
Active siteⁱ	507	Proton donor/acceptorBy similarity	1
Active siteⁱ	703	Proton donor/acceptorBy similarity	1

GO - Molecular functionⁱ

alpha,alpha-trehalase activity
Source: SGD
Inferred from mutant phenotypeⁱ
- 18065618
calcium ion binding Source: InterPro

Complete GO annotation...

GO - Biological processⁱ

trehalose catabolic process
Source: SGD
Inferred from mutant phenotypeⁱ
- 18065618

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase

Molecular function

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCycⁱ	MetaCyc:YBR001C-MONOMER. YEAST:YBR001C-MONOMER.

BioCycⁱ

MetaCyc:YBR001C-MONOMER.
YEAST:YBR001C-MONOMER.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH37. Glycoside Hydrolase Family 37.

CAZyⁱ

GH37. Glycoside Hydrolase Family 37.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Probable trehalase (EC:3.2.1.28) Alternative name(s): Alpha,alpha-trehalase Alpha,alpha-trehalose glucohydrolase
Gene namesⁱ	Name:NTH2 Ordered Locus Names:YBR001C ORF Names:YBR0106
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome II

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR001C.
Saccharomyces Genome Database More...SGDⁱ	S000000205. NTH2.

Subcellular locationⁱ

GO - Cellular componentⁱ

cytoplasm Source: InterPro

Complete GO annotation...

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000173800	1 – 780	Probable trehalaseAdd BLAST		780

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	52	PhosphoserineCombined sources	1
Modified residueⁱ	53	PhosphoserineCombined sources	1
Modified residueⁱ	88	PhosphothreonineCombined sources	1
Modified residueⁱ	112	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P35172.
PRIDEⁱ	P35172.

PTM databases

iPTMnetⁱ	P35172.

iPTMnetⁱ

P35172.

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	32702. 67 interactors.
Database of interacting proteins More...DIPⁱ	DIP-6841N.
IntActⁱ	P35172. 3 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-665495.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P35172.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	338 – 339	Substrate bindingBy similarity		2
Regionⁱ	384 – 386	Substrate bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 37 family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006949.
HOGENOMⁱ	HOG000192885.
InParanoidⁱ	P35172.
KEGG Orthology (KO) More...KOⁱ	K01194.
OMAⁱ	HDYGRKH.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C0UDQ.

Family and domain databases

InterProⁱ	View protein in InterPro IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. IPR011120. Trehalase_Ca-bd.
PANTHERⁱ	PTHR23403. PTHR23403. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01204. Trehalase. 1 hit. PF07492. Trehalase_Ca-bi. 1 hit.
PRINTSⁱ	PR00744. GLHYDRLASE37.
SUPFAMⁱ	SSF48208. SSF48208. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P35172-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MVDFLPKVTE INPPSEGNDG EDNIKPLSSG SEQRPLKEEG QQGGRRHHRR 
        60         70         80         90        100
LSSMHEYFDP FSNAEVYYGP ITDPRKQSKI HRLNRTRTMS VFNKVSDFKN 
       110        120        130        140        150
GMKDYTLKRR GSEDDSFLSS QGNRRFYIDN VDLALDELLA SEDTDKNHQI 
       160        170        180        190        200
TIEDTGPKVI KVGTANSNGF KHVNVRGTYM LSNLLQELTI AKSFGRHQIF 
       210        220        230        240        250
LDEARINENP VDRLSRLITT QFWTSLTRRV DLYNIAEIAR DSKIDTPGAK 
       260        270        280        290        300
NPRIYVPYNC PEQYEFYIQA SQMNPSLKLE VEYLPKDITA EYVKSLNDTP 
       310        320        330        340        350
GLLALAMEEH VNPSTGERSL VGYPYAVPGG RFNELYGWDS YLMALGLIES 
       360        370        380        390        400
NKVDVARGMV EHFIFEIDHY SKILNANRSY YLCRSQPPFL TDMALLVFEK 
       410        420        430        440        450
IGGKNNPNAI QLLKRAFRAA IKEYKEVWMS SPRLDSLTGL SCYHSDGIGI 
       460        470        480        490        500
PPETEPDHFD TILLPYAEKY NVTLEKLRYL YNEGMIKEPK LDAFFLHDRA 
       510        520        530        540        550
VRESGHDTTY RFEGVCAYLA TIDLNSLLYK YEKDIAFVIK EYFGNEYKDE 
       560        570        580        590        600
NDGTVTDSEH WEELAELRKT RINKYMWDED SGFFFYYNTK LKCRTSYESA 
       610        620        630        640        650
TTFWSLWAGL ATEEQAKITV EKALPQLEML GGLVACTEKS RGPISIDRPI 
       660        670        680        690        700
RQWDYPFGWA PHQILAWKGL SAYGYQQVAT RLAYRWLYMI TKSFVDYNGM 
       710        720        730        740        750
VVEKYDVTRG TDPHRVDAEY GNQGADFKGV ATEGFGWVNT SYLLGLKYMN 
       760        770        780
NHARRALAAC SPPLPFFNSL KPSEKKLYYL

Length:780

Mass (Da):89,679

Last modified:February 1, 1994 - v1

Checksum:ⁱB034B14A68B38C53

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z26494 Genomic DNA. Translation: CAA81270.1. Z35870 Genomic DNA. Translation: CAA84937.1. BK006936 Genomic DNA. Translation: DAA07122.1.
PIRⁱ	S44560.
NCBI Reference Sequences More...RefSeqⁱ	NP_009555.1. NM_001178349.1.

Genome annotation databases

EnsemblFungiⁱ	YBR001C; YBR001C; YBR001C.
GeneIDⁱ	852286.
KEGGⁱ	sce:YBR001C.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z26494 Genomic DNA. Translation: CAA81270.1. Z35870 Genomic DNA. Translation: CAA84937.1. BK006936 Genomic DNA. Translation: DAA07122.1.
PIRⁱ	S44560.
NCBI Reference Sequences More...RefSeqⁱ	NP_009555.1. NM_001178349.1.

3D structure databases

ProteinModelPortalⁱ	P35172.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32702. 67 interactors.
Database of interacting proteins More...DIPⁱ	DIP-6841N.
IntActⁱ	P35172. 3 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-665495.

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH37. Glycoside Hydrolase Family 37.

CAZyⁱ

GH37. Glycoside Hydrolase Family 37.

PTM databases

iPTMnetⁱ	P35172.

iPTMnetⁱ

P35172.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P35172.
PRIDEⁱ	P35172.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YBR001C; YBR001C; YBR001C.
GeneIDⁱ	852286.
KEGGⁱ	sce:YBR001C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR001C.
Saccharomyces Genome Database More...SGDⁱ	S000000205. NTH2.

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006949.
HOGENOMⁱ	HOG000192885.
InParanoidⁱ	P35172.
KEGG Orthology (KO) More...KOⁱ	K01194.
OMAⁱ	HDYGRKH.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C0UDQ.

Enzyme and pathway databases

BioCycⁱ	MetaCyc:YBR001C-MONOMER. YEAST:YBR001C-MONOMER.

BioCycⁱ

MetaCyc:YBR001C-MONOMER.
YEAST:YBR001C-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P35172.

PROⁱ

PR:P35172.

Family and domain databases

InterProⁱ	View protein in InterPro IPR008928. 6-hairpin_glycosidase-like. IPR001661. Glyco_hydro_37. IPR018232. Glyco_hydro_37_CS. IPR011120. Trehalase_Ca-bd.
PANTHERⁱ	PTHR23403. PTHR23403. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01204. Trehalase. 1 hit. PF07492. Trehalase_Ca-bi. 1 hit.
PRINTSⁱ	PR00744. GLHYDRLASE37.
SUPFAMⁱ	SSF48208. SSF48208. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00927. TREHALASE_1. 1 hit. PS00928. TREHALASE_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	TREB_YEAST
Accessionⁱ	P35172Primary (citable) accession number: P35172 Secondary accession number(s): D6VQ02
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
	Last sequence update:	February 1, 1994
	Last modified:	June 7, 2017
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P35172 (TREB_YEAST)

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Probable trehalase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ