P35170 (AROG_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

Name:	aroG
Synonyms:	aroF
Ordered Locus Names:	Cgl0990, cg1129

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Sequence similarities	Belongs to the class-I DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Phospho-2-dehydro-3-deoxyheptonate aldolase		PRO_0000140829
Experimental info
	Sequence conflict	109	1	I → T in AAA23292. Ref.1
	Sequence conflict	181	1	R → RR in AAA23292. Ref.1
	Sequence conflict	356	1	A → T in AAA23292. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35170 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 1EDA38DD26E29D58	FASTA	366	39,131
	10 20 30 40 50 60 MSSPVSLENA ASTSNKRVVA FHELPSPTDL IAANPLTPKQ ASKVEQDRQD IADIFAGDDD 70 80 90 100 110 120 RLVVVVGPCS VHDPEAAIDY ANRLAPLAKR LDQDLKIVMR VYFEKPRTIV GWKGLINDPH 130 140 150 160 170 180 LNETYDIPEG LRIARKVLID VVNLDLPVGC EFLEPNSPQY YADTVAWGAI GARTTESQVH 190 200 210 220 230 240 RQLASGMSMP IGFKNGTDGN IQVAVDAVQA AQNPHFFFGT SDDGALSVVE TAGNSNSHII 250 260 270 280 290 300 LRGGTSGPNH DAASVEAVVE KLGENARLMI DASHANSGKD HIRQVEVVRE IAEQISGGSE 310 320 330 340 350 360 AVAGIMIESF LVGGAQNLDP AKLRINGGEG LVYGQSVTDK CIDIDTTIDL LAELAAAVRE RRAAAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and nucleotide sequence of a Corynebacterium glutamicum 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase gene." Chen C.-C., Liao C.-C., Hsu W.-H. FEMS Microbiol. Lett. 107:223-230(1993) [PubMed: 8097175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCRC 18310.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07603 Genomic DNA. Translation: AAA23292.1. BA000036 Genomic DNA. Translation: BAB98383.1. BX927151 Genomic DNA. Translation: CAF19696.1.
PIR	I40837.
RefSeq	NP_600217.1. NC_003450.3. YP_225282.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P35170.
SMR	P35170. Positions 15-362.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1018979. 3343015.
GenomeReviews	Gene locus Cgl0990 in contig BA000036_GR. Gene locus cg1129 in contig BX927147_GR.
KEGG	cgb:cg1129. cgl:NCgl0950.
PATRIC	21494034. VBICorGlu203724_0974.
Phylogenomic databases
HOGENOM	HBG670536.
OMA	AGIMIES.
PhylomeDB	P35170.
ProtClustDB	PRK09261.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1129-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01626.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. AroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROG_CORGL

Accession

Primary (citable) accession number: P35170

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 11, 2002
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program