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Protein

Ribosomal large subunit pseudouridine synthase B

Gene

rluB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-2633 in 23S ribosomal RNA.

Catalytic activityi

23S rRNA uridine(2605) = 23S rRNA pseudouridine(2605).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei102NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU23160-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal large subunit pseudouridine synthase B (EC:5.4.99.22)
Alternative name(s):
23S rRNA pseudouridine(2605) synthase
rRNA pseudouridylate synthase B
rRNA-uridine isomerase B
Gene namesi
Name:rluB
Synonyms:ypuL
Ordered Locus Names:BSU23160
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000999791 – 244Ribosomal large subunit pseudouridine synthase BAdd BLAST244

Proteomic databases

PaxDbiP35159.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012716.

Structurei

3D structure databases

ProteinModelPortaliP35159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 68S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP35159.
KOiK06178.
OMAiHAFMANA.
PhylomeDBiP35159.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERLQKVIAH AGVASRRKAE ELIKEGKVKV NGKVVTELGV KVTGSDQIEV
60 70 80 90 100
NGLKVEREEP VYFLLYKPRG VISAAQDDKG RKVVTDFFKN IPQRIYPIGR
110 120 130 140 150
LDYDTSGLLL LTNDGEFANK LMHPKYEIDK TYVAKVKGIP PKELLRKLER
160 170 180 190 200
GIRLEEGKTA PAKAKLLSLD KKKQTSIIQL TIHEGRNRQV RRMFEAIGHE
210 220 230 240
VIKLKREEYA FLNLRGLHTG DARELTPHEV KRLRALADHG KNAF
Length:244
Mass (Da):27,738
Last modified:July 7, 2009 - v2
Checksum:iBD18C7E3A8A7C3D3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17R → S in AAA67493 (PubMed:7934829).Curated1
Sequence conflicti226 – 244TPHEV…GKNAF → RLTK in AAA67493 (PubMed:7934829).CuratedAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09228 Genomic DNA. Translation: AAA67493.1.
AL009126 Genomic DNA. Translation: CAB14248.2.
PIRiS45555.
RefSeqiNP_390197.2. NC_000964.3.
WP_004398815.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14248; CAB14248; BSU23160.
GeneIDi938955.
KEGGibsu:BSU23160.
PATRICi18976449. VBIBacSub10457_2415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09228 Genomic DNA. Translation: AAA67493.1.
AL009126 Genomic DNA. Translation: CAB14248.2.
PIRiS45555.
RefSeqiNP_390197.2. NC_000964.3.
WP_004398815.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP35159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012716.

Proteomic databases

PaxDbiP35159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14248; CAB14248; BSU23160.
GeneIDi938955.
KEGGibsu:BSU23160.
PATRICi18976449. VBIBacSub10457_2415.

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP35159.
KOiK06178.
OMAiHAFMANA.
PhylomeDBiP35159.

Enzyme and pathway databases

BioCyciBSUB:BSU23160-MONOMER.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLUB_BACSU
AccessioniPrimary (citable) accession number: P35159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 7, 2009
Last modified: October 5, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.