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P35154 (SCPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Segregation and condensation protein A
Gene names
Name:scpA
Synonyms:ypuG
Ordered Locus Names:BSU23220
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves. HAMAP-Rule MF_01805

Subunit structure

Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA. Ref.5

Subcellular location

Cytoplasm. Note: Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells. Ref.5

Sequence similarities

Belongs to the ScpA family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Chromosome partition
   Cellular componentCytoplasm
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

chromosome segregation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Segregation and condensation protein A HAMAP-Rule MF_01805
PRO_0000211080

Secondary structure

....... 251
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P35154 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9FE03F117D2919A0

FASTA25129,612
        10         20         30         40         50         60 
MEEYQVKIDT FEGPLDLLLH LINRLEIDIY DIPVAKITEQ YLLYVHTMRV LELDIASEYL 

        70         80         90        100        110        120 
VMAATLLSIK SRMLLPKQEE ELFEDELLEE EDPREELIEK LIEYRKYKDA AKDLKEREEE 

       130        140        150        160        170        180 
RQKSFTKPPS DLSEYAKEVK QSEQKLSVTV YDMIGAFQKV LKRKKINRPM ETTITRQDIP 

       190        200        210        220        230        240 
IEARMNEIVH SLKSRGTRIN FMDLFPYEQK EHLVVTFLAV LELMKNQLVL IEQEHNFSDI 

       250 
YITGSESIHG A 

« Hide

References

« Hide 'large scale' references
[1]"The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data."
Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.
Mol. Microbiol. 10:385-395(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein."
Mascarenhas J., Soppa J., Strunnikov A.V., Graumann P.L.
EMBO J. 21:3108-3118(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SCPB AND SMC.
[4]"Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB."
Soppa J., Kobayashi K., Noirot-Gros M.-F., Oesterhelt D., Ehrlich S.D., Dervyn E., Ogasawara N., Moriya S.
Mol. Microbiol. 45:59-71(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, INTERACTION WITH SCPB.
[5]"A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid and binds to DNA as a ring-like structure."
Volkov A., Mascarenhas J., Andrei-Selmer C., Ulrich H.D., Graumann P.L.
Mol. Cell. Biol. 23:5638-5650(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, HOMODIMERIZATION, INTERACTION WITH SMC AND SCPB.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L09228 Genomic DNA. Translation: AAA67487.1.
AL009126 Genomic DNA. Translation: CAB14254.1.
PIRS45549.
RefSeqNP_390203.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZGXX-ray3.40C/Z1-86[»]
ProteinModelPortalP35154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-52200N.
IntActP35154. 15 interactions.
STRING224308.BSU23220.

Proteomic databases

PaxDbP35154.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14254; CAB14254; BSU23220.
GeneID938950.
KEGGbsu:BSU23220.
PATRIC18976461. VBIBacSub10457_2421.

Organism-specific databases

GenoListBSU23220. [Micado]

Phylogenomic databases

eggNOGCOG1354.
HOGENOMHOG000242824.
KOK05896.
OMARKQNIDI.
OrthoDBEOG6CZQPX.
ProtClustDBPRK00104.

Enzyme and pathway databases

BioCycBSUB:BSU23220-MONOMER.

Family and domain databases

HAMAPMF_01805. ScpA.
InterProIPR003768. ScpA.
[Graphical view]
PfamPF02616. ScpA_ScpB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSCPA_BACSU
AccessionPrimary (citable) accession number: P35154
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList