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P35154

- SCPA_BACSU

UniProt

P35154 - SCPA_BACSU

Protein

Segregation and condensation protein A

Gene

scpA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. chromosome segregation Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition

    Enzyme and pathway databases

    BioCyciBSUB:BSU23220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Segregation and condensation protein A
    Gene namesi
    Name:scpA
    Synonyms:ypuG
    Ordered Locus Names:BSU23220
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU23220. [Micado]

    Subcellular locationi

    Cytoplasm 1 Publication
    Note: Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Segregation and condensation protein APRO_0000211080Add
    BLAST

    Proteomic databases

    PaxDbiP35154.

    Interactioni

    Subunit structurei

    Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    scpBP351553EBI-2121359,EBI-2121445
    smcP518346EBI-2121359,EBI-2121372

    Protein-protein interaction databases

    DIPiDIP-52200N.
    IntActiP35154. 15 interactions.
    STRINGi224308.BSU23220.

    Structurei

    Secondary structure

    1
    251
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi14 – 2916
    Beta strandi31 – 344
    Helixi39 – 7335

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ZGXX-ray3.40C/Z1-86[»]
    ProteinModelPortaliP35154.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ScpA family.Curated

    Phylogenomic databases

    eggNOGiCOG1354.
    HOGENOMiHOG000242824.
    KOiK05896.
    OMAiNILDIPM.
    OrthoDBiEOG6CZQPX.
    PhylomeDBiP35154.

    Family and domain databases

    HAMAPiMF_01805. ScpA.
    InterProiIPR003768. ScpA.
    [Graphical view]
    PfamiPF02616. ScpA_ScpB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35154-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEYQVKIDT FEGPLDLLLH LINRLEIDIY DIPVAKITEQ YLLYVHTMRV    50
    LELDIASEYL VMAATLLSIK SRMLLPKQEE ELFEDELLEE EDPREELIEK 100
    LIEYRKYKDA AKDLKEREEE RQKSFTKPPS DLSEYAKEVK QSEQKLSVTV 150
    YDMIGAFQKV LKRKKINRPM ETTITRQDIP IEARMNEIVH SLKSRGTRIN 200
    FMDLFPYEQK EHLVVTFLAV LELMKNQLVL IEQEHNFSDI YITGSESIHG 250
    A 251
    Length:251
    Mass (Da):29,612
    Last modified:February 1, 1994 - v1
    Checksum:i9FE03F117D2919A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09228 Genomic DNA. Translation: AAA67487.1.
    AL009126 Genomic DNA. Translation: CAB14254.1.
    PIRiS45549.
    RefSeqiNP_390203.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14254; CAB14254; BSU23220.
    GeneIDi938950.
    KEGGibsu:BSU23220.
    PATRICi18976461. VBIBacSub10457_2421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L09228 Genomic DNA. Translation: AAA67487.1 .
    AL009126 Genomic DNA. Translation: CAB14254.1 .
    PIRi S45549.
    RefSeqi NP_390203.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ZGX X-ray 3.40 C/Z 1-86 [» ]
    ProteinModelPortali P35154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-52200N.
    IntActi P35154. 15 interactions.
    STRINGi 224308.BSU23220.

    Proteomic databases

    PaxDbi P35154.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14254 ; CAB14254 ; BSU23220 .
    GeneIDi 938950.
    KEGGi bsu:BSU23220.
    PATRICi 18976461. VBIBacSub10457_2421.

    Organism-specific databases

    GenoListi BSU23220. [Micado ]

    Phylogenomic databases

    eggNOGi COG1354.
    HOGENOMi HOG000242824.
    KOi K05896.
    OMAi NILDIPM.
    OrthoDBi EOG6CZQPX.
    PhylomeDBi P35154.

    Enzyme and pathway databases

    BioCyci BSUB:BSU23220-MONOMER.

    Family and domain databases

    HAMAPi MF_01805. ScpA.
    InterProi IPR003768. ScpA.
    [Graphical view ]
    Pfami PF02616. ScpA_ScpB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data."
      Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.
      Mol. Microbiol. 10:385-395(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein."
      Mascarenhas J., Soppa J., Strunnikov A.V., Graumann P.L.
      EMBO J. 21:3108-3118(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCPB AND SMC.
    4. "Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB."
      Soppa J., Kobayashi K., Noirot-Gros M.-F., Oesterhelt D., Ehrlich S.D., Dervyn E., Ogasawara N., Moriya S.
      Mol. Microbiol. 45:59-71(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, INTERACTION WITH SCPB.
    5. "A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid and binds to DNA as a ring-like structure."
      Volkov A., Mascarenhas J., Andrei-Selmer C., Ulrich H.D., Graumann P.L.
      Mol. Cell. Biol. 23:5638-5650(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, HOMODIMERIZATION, INTERACTION WITH SMC AND SCPB.

    Entry informationi

    Entry nameiSCPA_BACSU
    AccessioniPrimary (citable) accession number: P35154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3