Segregation and condensation protein A - Bacillus subtilis (strain 168)

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P35154 (SCPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Segregation and condensation protein A

Gene names

Name:	scpA
Synonyms:	ypuG
Ordered Locus Names:	BSU23220

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in chromosomal partition during cell division. May act via the formation of a condensin-like complex containing Smc and ScpB that pull DNA away from mid-cell into both cell halves. HAMAP-Rule MF_01805
Subunit structure	Component of a cohesin-like complex composed of ScpA, ScpB and the Smc homodimer, in which ScpA and ScpB bind to the head domain of Smc. The presence of the three proteins is required for the association of the complex with DNA. Ref.5
Subcellular location	Cytoplasm. Note: Associated with two foci at the outer edges of the nucleoid region in young cells, and at four foci within both cell halves in older cells. Ref.5
Sequence similarities	Belongs to the ScpA family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
scpB	P35155	3	EBI-2121359,EBI-2121445
smc	P51834	6	EBI-2121359,EBI-2121372

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

Segregation and condensation protein A HAMAP-Rule MF_01805

PRO_0000211080

Secondary structure

251

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P35154 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9FE03F117D2919A0
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FASTA

251

29,612

        10         20         30         40         50         60 
MEEYQVKIDT FEGPLDLLLH LINRLEIDIY DIPVAKITEQ YLLYVHTMRV LELDIASEYL 

        70         80         90        100        110        120 
VMAATLLSIK SRMLLPKQEE ELFEDELLEE EDPREELIEK LIEYRKYKDA AKDLKEREEE 

       130        140        150        160        170        180 
RQKSFTKPPS DLSEYAKEVK QSEQKLSVTV YDMIGAFQKV LKRKKINRPM ETTITRQDIP 

       190        200        210        220        230        240 
IEARMNEIVH SLKSRGTRIN FMDLFPYEQK EHLVVTFLAV LELMKNQLVL IEQEHNFSDI 

       250 
YITGSESIHG A

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data." Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P. Mol. Microbiol. 10:385-395(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Cell cycle-dependent localization of two novel prokaryotic chromosome segregation and condensation proteins in Bacillus subtilis that interact with SMC protein." Mascarenhas J., Soppa J., Strunnikov A.V., Graumann P.L. EMBO J. 21:3108-3118(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SCPB AND SMC.
[4]	"Discovery of two novel families of proteins that are proposed to interact with prokaryotic SMC proteins, and characterization of the Bacillus subtilis family members ScpA and ScpB." Soppa J., Kobayashi K., Noirot-Gros M.-F., Oesterhelt D., Ehrlich S.D., Dervyn E., Ogasawara N., Moriya S. Mol. Microbiol. 45:59-71(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, INTERACTION WITH SCPB.
[5]	"A prokaryotic condensin/cohesin-like complex can actively compact chromosomes from a single position on the nucleoid and binds to DNA as a ring-like structure." Volkov A., Mascarenhas J., Andrei-Selmer C., Ulrich H.D., Graumann P.L. Mol. Cell. Biol. 23:5638-5650(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, HOMODIMERIZATION, INTERACTION WITH SMC AND SCPB.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L09228 Genomic DNA. Translation: AAA67487.1.
AL009126 Genomic DNA. Translation: CAB14254.1.

PIR

S45549.

RefSeq

NP_390203.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ZGX	X-ray	3.40	C/Z	1-86	[»]

ProteinModelPortal

P35154.

ModBase

Search...

Protein-protein interaction databases

IntAct

P35154. 15 interactions.

STRING

224308.BSU23220.

Proteomic databases

PaxDb

P35154.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB14254; CAB14254; BSU23220.

GeneID

938950.

KEGG

bsu:BSU23220.

PATRIC

18976461. VBIBacSub10457_2421.

Organism-specific databases

GenoList

BSU23220. [Micado]

Phylogenomic databases

eggNOG

COG1354.

HOGENOM

HOG000242824.

K05896.

OMA

RKQNIDI.

ProtClustDB

PRK00104.

Enzyme and pathway databases

BioCyc

BSUB:BSU23220-MONOMER.

Family and domain databases

HAMAP

MF_01805. ScpA.

InterPro

IPR003768. ScpA.
[Graphical view]

Pfam

PF02616. ScpA_ScpB. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

SCPA_BACSU

Accession

Primary (citable) accession number: P35154

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents