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P35136 (SERA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-3-phosphoglycerate dehydrogenase
Short name=PGDH
EC=1.1.1.95
Gene names
Name:serA
Ordered Locus Names:BSU23070
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH.

2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.

Enzyme regulation

In bacteria displays feedback inhibition by L-serine.

Pathway

Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Contains 1 ACT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525D-3-phosphoglycerate dehydrogenase
PRO_0000075998

Regions

Domain451 – 52272ACT
Nucleotide binding148 – 1492NAD By similarity
Nucleotide binding227 – 2293NAD By similarity
Nucleotide binding276 – 2794NAD By similarity

Sites

Active site2291 By similarity
Active site2581 By similarity
Active site2761Proton donor By similarity
Binding site1681NAD By similarity
Binding site2001NAD; via carbonyl oxygen By similarity
Binding site2531NAD By similarity

Experimental info

Sequence conflict157 – 1582AR → RG in AAC83943. Ref.1
Sequence conflict157 – 1582AR → RG in AAA67502. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P35136 [UniParc].

Last modified July 7, 2009. Version 3.
Checksum: 9B026BF481FBC1D2

FASTA52557,129
        10         20         30         40         50         60 
MFRVLVSDKM SNDGLQPLIE SDFIEIVQKN VADAEDELHT FDALLVRSAT KVTEDLFNKM 

        70         80         90        100        110        120 
TSLKIVGRAG VGVDNIDIDE ATKHGVIVIN APNGNTISTA EHTFAMISSL MRHIPQANIS 

       130        140        150        160        170        180 
VKSREWNRTA YVGSELYGKT LGIVGLGRIG SEIAQRARAF GMTVHVFDPF LTEERAKKIG 

       190        200        210        220        230        240 
VNSRTFEEVL ESADIITVHT PLTKETKGLL NKETIAKTKK GVRLINCARG GIIDEAALLE 

       250        260        270        280        290        300 
ALENGHVAGA ALDVFEVEPP VDNKLVDHPL VIATPHLGAS TKEAQLNVAA QVSEEVLQFA 

       310        320        330        340        350        360 
KGLPVMSAIN LPAMTKDEFA KIKPYHQIAG KIGSLVSQCM KEPVQDVAIQ YEGTIAKLET 

       370        380        390        400        410        420 
SFITKALLSG FLKPRVDSTV NEVNAGGVAK ERGISFSEKI SSSESGYDNC ISVKVTGDRS 

       430        440        450        460        470        480 
TFTVTATYIP HFGERIVEIN GFNIDFYPTG HLVYIQHQDT TGVIGRVGRI LGDNDINIAT 

       490        500        510        520 
MQVGRKEKGG EAIMMLSFDR HLEDKIVKEL TNVPDIVSVK LIDLP

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome."
Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P.
Microbiology 142:2005-2016(1996) [PubMed: 8760912] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 157-158.
[4]"The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data."
Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P.
Mol. Microbiol. 10:385-395(1993) [PubMed: 7934829] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-525.
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L47648 Genomic DNA. Translation: AAC83943.1.
AL009126 Genomic DNA. Translation: CAB14239.2.
L09228 Genomic DNA. Translation: AAA67502.1.
PIRC69705.
RefSeqNP_390188.2. NC_000964.3.

3D structure databases

ProteinModelPortalP35136.
SMRP35136. Positions 1-524.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000002415; EBBACP00000002415; EBBACG00000002410.
GeneID938964.
GenomeReviewsGene locus BSU23070 in contig AL009126_GR.
KEGGbsu:BSU23070.
NMPDRfig|224308.1.peg.2311.
PATRIC18976431. VBIBacSub10457_2406.

Organism-specific databases

GenoListBSU23070. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001763.
HOGENOMHBG731446.
PhylomeDBP35136.
ProtClustDBPRK13581.

Enzyme and pathway databases

BioCycBSUB:BSU23070-MONOMER.

Family and domain databases

InterProIPR002912. ACT-bd.
IPR006236. D-3-Phosphoglycerate_DH.
IPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK00058.
PANTHERPTHR10996:SF20. D3PG_Deh. 1 hit.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
PF01842. ACT. 1 hit.
[Graphical view]
TIGRFAMsTIGR01327. PGDH. 1 hit.
PROSITEPS00065. D_2_HYDROXYACID_DH_1. 1 hit.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSERA_BACSU
AccessionPrimary (citable) accession number: P35136
Secondary accession number(s): O32011
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: July 7, 2009
Last modified: January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents