Reviewed, UniProtKB/Swiss-Prot P35136 (SERA_BACSU)

Names and origin

Protein names

Recommended name:
    D-3-phosphoglycerate dehydrogenase
      Short name=PGDH
    EC=1.1.1.95

Gene names

Name:	serA
Ordered Locus Names:	BSU23070

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. 2-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH.
Enzyme regulation	In bacteria displays feedback inhibition by L-serine.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glyceric acid: step 1/3.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro amino acid binding Inferred from electronic annotation. Source: InterPro phosphoglycerate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	D-3-phosphoglycerate dehydrogenase		PRO_0000075998
Regions
	Domain	451 – 522	72	ACT
Sites
	Active site	229	1	By similarity
	Active site	258	1	By similarity
	Active site	276	1	Proton donor By similarity
Experimental info
	Sequence conflict	157 – 158	2	AR → RG in AAC83943. Ref.1
	Sequence conflict	157 – 158	2	AR → RG in AAA67502. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P35136-1 [UniParc]. Last modified July 7, 2009. Version 3. Checksum: 9B026BF481FBC1D2	FASTA	525	57,129
	10 20 30 40 50 60 MFRVLVSDKM SNDGLQPLIE SDFIEIVQKN VADAEDELHT FDALLVRSAT KVTEDLFNKM 70 80 90 100 110 120 TSLKIVGRAG VGVDNIDIDE ATKHGVIVIN APNGNTISTA EHTFAMISSL MRHIPQANIS 130 140 150 160 170 180 VKSREWNRTA YVGSELYGKT LGIVGLGRIG SEIAQRARAF GMTVHVFDPF LTEERAKKIG 190 200 210 220 230 240 VNSRTFEEVL ESADIITVHT PLTKETKGLL NKETIAKTKK GVRLINCARG GIIDEAALLE 250 260 270 280 290 300 ALENGHVAGA ALDVFEVEPP VDNKLVDHPL VIATPHLGAS TKEAQLNVAA QVSEEVLQFA 310 320 330 340 350 360 KGLPVMSAIN LPAMTKDEFA KIKPYHQIAG KIGSLVSQCM KEPVQDVAIQ YEGTIAKLET 370 380 390 400 410 420 SFITKALLSG FLKPRVDSTV NEVNAGGVAK ERGISFSEKI SSSESGYDNC ISVKVTGDRS 430 440 450 460 470 480 TFTVTATYIP HFGERIVEIN GFNIDFYPTG HLVYIQHQDT TGVIGRVGRI LGDNDINIAT 490 500 510 520 MQVGRKEKGG EAIMMLSFDR HLEDKIVKEL TNVPDIVSVK LIDLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome." Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D., Serror P. Microbiology 142:2005-2016(1996) [PubMed: 8760912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 157-158.
[4]	"The organization of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data." Sorokin A.V., Zumstein E., Azevedo V., Ehrlich S.D., Serror P. Mol. Microbiol. 10:385-395(1993) [PubMed: 7934829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-525. Strain: 168 / Marburg.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L47648 Genomic DNA. Translation: AAC83943.1. AL009126 Genomic DNA. Translation: CAB14239.2. L09228 Genomic DNA. Translation: AAA67502.1.
PIR	C69705.
RefSeq	NP_390188.1.
3D structure databases
HSSP	HSSP built from PDB template 1HKU based on UniProtKB Q9Z2F5.
ModBase	Search...
Genome annotation databases
GeneID	938964.
GenomeReviews	Gene locus BSU23070 in contig AL009126_GR.
KEGG	bsu:BSU23070.
NMPDR	fig|224308.1.peg.2311.
Organism-specific databases
SubtiList	BG10509. serA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P35136.
OMA	P35136. DMPGIIG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2306-MON.
BRENDA	1.1.1.95. 150.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR006236. D-3-Phosphoglycerate_DH. IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR015508. D3PG_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10996:SF20. D3PG_Deh. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. PF01842. ACT. 1 hit. [Graphical view]
TIGRFAMs	TIGR01327. PGDH. 1 hit.
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_BACSU

Accession

Primary (citable) accession number: P35136
Secondary accession number(s): O32011

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	July 7, 2009
Last modified:	July 7, 2009
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)