ID   UBC4_SOLLC              Reviewed;         148 AA.
AC   P35135;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   27-MAR-2024, entry version 127.
DE   RecName: Full=Ubiquitin-conjugating enzyme E2-17 kDa;
DE            EC=2.3.2.23;
DE   AltName: Full=E2 ubiquitin-conjugating enzyme;
DE   AltName: Full=Ubiquitin carrier protein;
DE   AltName: Full=Ubiquitin-protein ligase;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Root;
RA   Bird D.M., Wilson M.A.;
RT   "Expression of an ubiquitin carrier protein gene in tomato root cells
RT   infected with the nematode Meloidogyne incognita.";
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other
CC       proteins. Mediates the selective degradation of short-lived and
CC       abnormal proteins. {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine +
CC         [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-
CC         activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-
CC         conjugating enzyme]-L-cysteine.; EC=2.3.2.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00388, ECO:0000255|PROSITE-
CC         ProRule:PRU10133};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00388}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L23762; AAA34125.1; -; mRNA.
DR   RefSeq; NP_001234247.1; NM_001247318.2.
DR   RefSeq; NP_001294911.1; NM_001307982.1.
DR   RefSeq; NP_001294913.1; NM_001307984.1.
DR   RefSeq; XP_019069675.1; XM_019214130.1.
DR   AlphaFoldDB; P35135; -.
DR   SMR; P35135; -.
DR   STRING; 4081.P35135; -.
DR   PaxDb; 4081-Solyc06g007510-2-1; -.
DR   EnsemblPlants; Solyc06g007510.3.1; Solyc06g007510.3.1; Solyc06g007510.3.
DR   EnsemblPlants; Solyc06g082600.3.1; Solyc06g082600.3.1; Solyc06g082600.3.
DR   GeneID; 100301932; -.
DR   GeneID; 101249087; -.
DR   Gramene; Solyc06g007510.3.1; Solyc06g007510.3.1; Solyc06g007510.3.
DR   Gramene; Solyc06g082600.3.1; Solyc06g082600.3.1; Solyc06g082600.3.
DR   KEGG; sly:100301932; -.
DR   KEGG; sly:101249087; -.
DR   eggNOG; KOG0417; Eukaryota.
DR   HOGENOM; CLU_030988_13_3_1; -.
DR   InParanoid; P35135; -.
DR   OMA; FCELNRE; -.
DR   OrthoDB; 333234at2759; -.
DR   PhylomeDB; P35135; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000004994; Chromosome 6.
DR   ExpressionAtlas; P35135; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:UniProtKB-EC.
DR   GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd00195; UBCc; 1.
DR   Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   InterPro; IPR023313; UBQ-conjugating_AS.
DR   InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR   PANTHER; PTHR24068:SF506; AMINOACYLTRANSFERASE, E1 UBIQUITIN-ACTIVATING ENZYME-RELATED; 1.
DR   PANTHER; PTHR24068; UBIQUITIN-CONJUGATING ENZYME E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   SMART; SM00212; UBCc; 1.
DR   SUPFAM; SSF54495; UBC-like; 1.
DR   PROSITE; PS00183; UBC_1; 1.
DR   PROSITE; PS50127; UBC_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Transferase;
KW   Ubl conjugation pathway.
FT   CHAIN           1..148
FT                   /note="Ubiquitin-conjugating enzyme E2-17 kDa"
FT                   /id="PRO_0000082580"
FT   DOMAIN          1..147
FT                   /note="UBC core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388"
FT   ACT_SITE        85
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00388,
FT                   ECO:0000255|PROSITE-ProRule:PRU10133"
SQ   SEQUENCE   148 AA;  16522 MW;  4247FF721B98059F CRC64;
     MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP TDSPYAGGVF LVSIHFPPDY
     PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS KVLLSICSLL TDPNPDDPLV
     PEIAHMYKTD RAKYETTARS WTQKYAMG
//