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P35133

- UBC10_ARATH

UniProt

P35133 - UBC10_ARATH

Protein

Ubiquitin-conjugating enzyme E2 10

Gene

UBC10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. ubiquitin protein ligase binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G53300-MONOMER.
    ARA:GQT-1310-MONOMER.
    ARA:GQT-1311-MONOMER.
    ARA:GQT-1312-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 10 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 10/12
    Ubiquitin-conjugating enzyme E2-17 kDa 10/12
    Ubiquitin-protein ligase 10/12
    Gene namesi
    Name:UBC10
    Synonyms:UBC12
    Ordered Locus Names:At5g53300
    ORF Names:K19E1.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G53300.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148Ubiquitin-conjugating enzyme E2 10PRO_0000082578Add
    BLAST

    Proteomic databases

    PaxDbiP35133.
    PRIDEiP35133.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed with the highest levels in rosette leaves, roots and petals.1 Publication

    Gene expression databases

    GenevestigatoriP35133.

    Interactioni

    Subunit structurei

    Interacts with CHIP and the E3 ubiquitin ligase BB.2 Publications

    Protein-protein interaction databases

    BioGridi20656. 10 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP35133.
    SMRiP35133. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    InParanoidiP35133.
    KOiK06689.
    OMAiSAGPCED.
    PhylomeDBiP35133.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P35133-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SESPYAGGVF    50
    LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS 100
    KVLLSICSLL TDPNPDDPLV PEIAHMYKTD KNKYESTARS WTQKYAMG 148
    Length:148
    Mass (Da):16,537
    Last modified:February 1, 1994 - v1
    Checksum:i2B83EDC1AD2AE657
    GO

    Sequence cautioni

    The sequence Z14993 differs from that shown. Reason: Artifacts of PCR amplification. Originally thought to be UBC12 isoform.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231A → T in Z14993. (PubMed:8220461)Curated
    Sequence conflicti140 – 1401S → T in Z14993. (PubMed:8220461)Curated
    Sequence conflicti144 – 1441K → E in AAM63450. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14991 mRNA. Translation: CAA78715.1.
    L00640 mRNA. Translation: AAA32895.1.
    Z14993 Genomic DNA. No translation available.
    DQ027024 mRNA. Translation: AAY44850.1.
    AB013388 Genomic DNA. Translation: BAB09792.1.
    CP002688 Genomic DNA. Translation: AED96334.1.
    CP002688 Genomic DNA. Translation: AED96335.1.
    CP002688 Genomic DNA. Translation: AED96337.1.
    AF324718 mRNA. Translation: AAG40069.1.
    AF325005 mRNA. Translation: AAG40357.1.
    AF326872 mRNA. Translation: AAG41454.1.
    AY039566 mRNA. Translation: AAK62621.1.
    AY065059 mRNA. Translation: AAL57693.1.
    AY113937 mRNA. Translation: AAM44985.1.
    AY129488 mRNA. Translation: AAM91074.1.
    AY086447 mRNA. Translation: AAM63450.1.
    PIRiS32672.
    RefSeqiNP_001190528.1. NM_001203599.1. [P35133-1]
    NP_568788.1. NM_124709.2. [P35133-1]
    NP_851181.1. NM_180850.2. [P35133-1]
    UniGeneiAt.24357.

    Genome annotation databases

    EnsemblPlantsiAT5G53300.1; AT5G53300.1; AT5G53300. [P35133-1]
    AT5G53300.2; AT5G53300.2; AT5G53300. [P35133-1]
    AT5G53300.4; AT5G53300.4; AT5G53300. [P35133-1]
    GeneIDi835411.
    KEGGiath:AT5G53300.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14991 mRNA. Translation: CAA78715.1 .
    L00640 mRNA. Translation: AAA32895.1 .
    Z14993 Genomic DNA. No translation available.
    DQ027024 mRNA. Translation: AAY44850.1 .
    AB013388 Genomic DNA. Translation: BAB09792.1 .
    CP002688 Genomic DNA. Translation: AED96334.1 .
    CP002688 Genomic DNA. Translation: AED96335.1 .
    CP002688 Genomic DNA. Translation: AED96337.1 .
    AF324718 mRNA. Translation: AAG40069.1 .
    AF325005 mRNA. Translation: AAG40357.1 .
    AF326872 mRNA. Translation: AAG41454.1 .
    AY039566 mRNA. Translation: AAK62621.1 .
    AY065059 mRNA. Translation: AAL57693.1 .
    AY113937 mRNA. Translation: AAM44985.1 .
    AY129488 mRNA. Translation: AAM91074.1 .
    AY086447 mRNA. Translation: AAM63450.1 .
    PIRi S32672.
    RefSeqi NP_001190528.1. NM_001203599.1. [P35133-1 ]
    NP_568788.1. NM_124709.2. [P35133-1 ]
    NP_851181.1. NM_180850.2. [P35133-1 ]
    UniGenei At.24357.

    3D structure databases

    ProteinModelPortali P35133.
    SMRi P35133. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20656. 10 interactions.

    Proteomic databases

    PaxDbi P35133.
    PRIDEi P35133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G53300.1 ; AT5G53300.1 ; AT5G53300 . [P35133-1 ]
    AT5G53300.2 ; AT5G53300.2 ; AT5G53300 . [P35133-1 ]
    AT5G53300.4 ; AT5G53300.4 ; AT5G53300 . [P35133-1 ]
    GeneIDi 835411.
    KEGGi ath:AT5G53300.

    Organism-specific databases

    TAIRi AT5G53300.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    InParanoidi P35133.
    KOi K06689.
    OMAi SAGPCED.
    PhylomeDBi P35133.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G53300-MONOMER.
    ARA:GQT-1310-MONOMER.
    ARA:GQT-1311-MONOMER.
    ARA:GQT-1312-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori P35133.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
      Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
      Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
      Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
      DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. "The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner."
      Disch S., Anastasiou E., Sharma V.K., Laux T., Fletcher J.C., Lenhard M.
      Curr. Biol. 16:272-279(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BB.
    8. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
      Luo J., Shen G., Yan J., He C., Zhang H.
      Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHIP.

    Entry informationi

    Entry nameiUBC10_ARATH
    AccessioniPrimary (citable) accession number: P35133
    Secondary accession number(s): P56617, Q4TZ00, Q8LCR4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3