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Protein

Ubiquitin-conjugating enzyme E2 10

Gene

UBC10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin protein ligase binding Source: UniProtKB
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-5689896 Ovarian tumor domain proteases
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-9033241 Peroxisomal protein import
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 10 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 10
Ubiquitin carrier protein 10/12
Ubiquitin-conjugating enzyme E2-17 kDa 10/12
Ubiquitin-protein ligase 10/12
Gene namesi
Name:UBC10
Synonyms:UBC12
Ordered Locus Names:At5g53300
ORF Names:K19E1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G53300
TAIRilocus:2154207 AT5G53300

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825781 – 148Ubiquitin-conjugating enzyme E2 10Add BLAST148

Proteomic databases

PaxDbiP35133
PRIDEiP35133

Expressioni

Tissue specificityi

Ubiquitously expressed with the highest levels in rosette leaves, roots and petals.1 Publication

Gene expression databases

ExpressionAtlasiP35133 baseline and differential
GenevisibleiP35133 AT

Interactioni

Subunit structurei

Interacts with CHIP and the E3 ubiquitin ligase BB.2 Publications

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi20656, 11 interactors
STRINGi3702.AT5G53300.1

Structurei

3D structure databases

ProteinModelPortaliP35133
SMRiP35133
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233455
InParanoidiP35133
OMAiNDLGRDP
OrthoDBiEOG09360PU6
PhylomeDBiP35133

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P35133-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SESPYAGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD KNKYESTARS WTQKYAMG
Length:148
Mass (Da):16,537
Last modified:February 1, 1994 - v1
Checksum:i2B83EDC1AD2AE657
GO

Sequence cautioni

The sequence Z14993 differs from that shown. Artifacts of PCR amplification. Originally thought to be UBC12 isoform.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23A → T in Z14993 (PubMed:8220461).Curated1
Sequence conflicti140S → T in Z14993 (PubMed:8220461).Curated1
Sequence conflicti144K → E in AAM63450 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14991 mRNA Translation: CAA78715.1
L00640 mRNA Translation: AAA32895.1
Z14993 Genomic DNA No translation available.
DQ027024 mRNA Translation: AAY44850.1
AB013388 Genomic DNA Translation: BAB09792.1
CP002688 Genomic DNA Translation: AED96334.1
CP002688 Genomic DNA Translation: AED96335.1
CP002688 Genomic DNA Translation: AED96337.1
AF324718 mRNA Translation: AAG40069.1
AF325005 mRNA Translation: AAG40357.1
AF326872 mRNA Translation: AAG41454.1
AY039566 mRNA Translation: AAK62621.1
AY065059 mRNA Translation: AAL57693.1
AY113937 mRNA Translation: AAM44985.1
AY129488 mRNA Translation: AAM91074.1
AY086447 mRNA Translation: AAM63450.1
PIRiS32672
RefSeqiNP_001190528.1, NM_001203599.1 [P35133-1]
NP_568788.1, NM_124709.2 [P35133-1]
NP_851181.1, NM_180850.3 [P35133-1]
UniGeneiAt.24357

Genome annotation databases

EnsemblPlantsiAT5G53300.1; AT5G53300.1; AT5G53300 [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300 [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300 [P35133-1]
GeneIDi835411
GrameneiAT5G53300.1; AT5G53300.1; AT5G53300 [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300 [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300 [P35133-1]
KEGGiath:AT5G53300

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBC10_ARATH
AccessioniPrimary (citable) accession number: P35133
Secondary accession number(s): P56617, Q4TZ00, Q8LCR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health