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Protein

Ubiquitin-conjugating enzyme E2 10

Gene

UBC10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin protein ligase binding Source: UniProtKB
  4. ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G53300-MONOMER.
ARA:GQT-1310-MONOMER.
ARA:GQT-1311-MONOMER.
ARA:GQT-1312-MONOMER.
ReactomeiREACT_273097. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_284122. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294737. Senescence-Associated Secretory Phenotype (SASP).
REACT_295391. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_299093. Regulation of APC/C activators between G1/S and early anaphase.
REACT_301780. APC/C:Cdc20 mediated degradation of Securin.
REACT_305944. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_314276. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_318090. Separation of Sister Chromatids.
REACT_319658. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_336371. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_353749. APC-Cdc20 mediated degradation of Nek2A.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 10 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 10/12
Ubiquitin-conjugating enzyme E2-17 kDa 10/12
Ubiquitin-protein ligase 10/12
Gene namesi
Name:UBC10
Synonyms:UBC12
Ordered Locus Names:At5g53300
ORF Names:K19E1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G53300.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 10PRO_0000082578Add
BLAST

Proteomic databases

PaxDbiP35133.
PRIDEiP35133.

Expressioni

Tissue specificityi

Ubiquitously expressed with the highest levels in rosette leaves, roots and petals.1 Publication

Gene expression databases

GenevestigatoriP35133.

Interactioni

Subunit structurei

Interacts with CHIP and the E3 ubiquitin ligase BB.2 Publications

Protein-protein interaction databases

BioGridi20656. 10 interactions.

Structurei

3D structure databases

ProteinModelPortaliP35133.
SMRiP35133. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiP35133.
KOiK06689.
OMAiSAGPCED.
PhylomeDBiP35133.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P35133-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SESPYAGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD KNKYESTARS WTQKYAMG
Length:148
Mass (Da):16,537
Last modified:February 1, 1994 - v1
Checksum:i2B83EDC1AD2AE657
GO

Sequence cautioni

The sequence Z14993 differs from that shown.Artifacts of PCR amplification. Originally thought to be UBC12 isoform.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231A → T in Z14993 (PubMed:8220461).Curated
Sequence conflicti140 – 1401S → T in Z14993 (PubMed:8220461).Curated
Sequence conflicti144 – 1441K → E in AAM63450 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14991 mRNA. Translation: CAA78715.1.
L00640 mRNA. Translation: AAA32895.1.
Z14993 Genomic DNA. No translation available.
DQ027024 mRNA. Translation: AAY44850.1.
AB013388 Genomic DNA. Translation: BAB09792.1.
CP002688 Genomic DNA. Translation: AED96334.1.
CP002688 Genomic DNA. Translation: AED96335.1.
CP002688 Genomic DNA. Translation: AED96337.1.
AF324718 mRNA. Translation: AAG40069.1.
AF325005 mRNA. Translation: AAG40357.1.
AF326872 mRNA. Translation: AAG41454.1.
AY039566 mRNA. Translation: AAK62621.1.
AY065059 mRNA. Translation: AAL57693.1.
AY113937 mRNA. Translation: AAM44985.1.
AY129488 mRNA. Translation: AAM91074.1.
AY086447 mRNA. Translation: AAM63450.1.
PIRiS32672.
RefSeqiNP_001190528.1. NM_001203599.1. [P35133-1]
NP_568788.1. NM_124709.2. [P35133-1]
NP_851181.1. NM_180850.2. [P35133-1]
UniGeneiAt.24357.

Genome annotation databases

EnsemblPlantsiAT5G53300.1; AT5G53300.1; AT5G53300. [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300. [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300. [P35133-1]
GeneIDi835411.
KEGGiath:AT5G53300.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14991 mRNA. Translation: CAA78715.1.
L00640 mRNA. Translation: AAA32895.1.
Z14993 Genomic DNA. No translation available.
DQ027024 mRNA. Translation: AAY44850.1.
AB013388 Genomic DNA. Translation: BAB09792.1.
CP002688 Genomic DNA. Translation: AED96334.1.
CP002688 Genomic DNA. Translation: AED96335.1.
CP002688 Genomic DNA. Translation: AED96337.1.
AF324718 mRNA. Translation: AAG40069.1.
AF325005 mRNA. Translation: AAG40357.1.
AF326872 mRNA. Translation: AAG41454.1.
AY039566 mRNA. Translation: AAK62621.1.
AY065059 mRNA. Translation: AAL57693.1.
AY113937 mRNA. Translation: AAM44985.1.
AY129488 mRNA. Translation: AAM91074.1.
AY086447 mRNA. Translation: AAM63450.1.
PIRiS32672.
RefSeqiNP_001190528.1. NM_001203599.1. [P35133-1]
NP_568788.1. NM_124709.2. [P35133-1]
NP_851181.1. NM_180850.2. [P35133-1]
UniGeneiAt.24357.

3D structure databases

ProteinModelPortaliP35133.
SMRiP35133. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20656. 10 interactions.

Proteomic databases

PaxDbiP35133.
PRIDEiP35133.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G53300.1; AT5G53300.1; AT5G53300. [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300. [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300. [P35133-1]
GeneIDi835411.
KEGGiath:AT5G53300.

Organism-specific databases

TAIRiAT5G53300.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiP35133.
KOiK06689.
OMAiSAGPCED.
PhylomeDBiP35133.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciARA:AT5G53300-MONOMER.
ARA:GQT-1310-MONOMER.
ARA:GQT-1311-MONOMER.
ARA:GQT-1312-MONOMER.
ReactomeiREACT_273097. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_284122. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_294737. Senescence-Associated Secretory Phenotype (SASP).
REACT_295391. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_299093. Regulation of APC/C activators between G1/S and early anaphase.
REACT_301780. APC/C:Cdc20 mediated degradation of Securin.
REACT_305944. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_314276. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_318090. Separation of Sister Chromatids.
REACT_319658. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_336371. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_353749. APC-Cdc20 mediated degradation of Nek2A.

Miscellaneous databases

PROiP35133.

Gene expression databases

GenevestigatoriP35133.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
    Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
    Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner."
    Disch S., Anastasiou E., Sharma V.K., Laux T., Fletcher J.C., Lenhard M.
    Curr. Biol. 16:272-279(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BB.
  8. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
    Luo J., Shen G., Yan J., He C., Zhang H.
    Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHIP.

Entry informationi

Entry nameiUBC10_ARATH
AccessioniPrimary (citable) accession number: P35133
Secondary accession number(s): P56617, Q4TZ00, Q8LCR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 29, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.