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P35133 (UBC10_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 10

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 10/12
Ubiquitin-conjugating enzyme E2-17 kDa 10/12
Ubiquitin-protein ligase 10/12
Gene names
Name:UBC10
Synonyms:UBC12
Ordered Locus Names:At5g53300
ORF Names:K19E1.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length148 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Ref.2

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with CHIP and the E3 ubiquitin ligase BB. Ref.7 Ref.8

Tissue specificity

Ubiquitously expressed with the highest levels in rosette leaves, roots and petals. Ref.2

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence caution

The sequence Z14993 differs from that shown. Reason: Artifacts of PCR amplification. Originally thought to be UBC12 isoform.

Ontologies

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P35133-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 148148Ubiquitin-conjugating enzyme E2 10
PRO_0000082578

Sites

Active site851Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict231A → T in Z14993. Ref.1
Sequence conflict1401S → T in Z14993. Ref.1
Sequence conflict1441K → E in AAM63450. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 2B83EDC1AD2AE657

FASTA14816,537
        10         20         30         40         50         60 
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SESPYAGGVF LVTIHFPPDY 

        70         80         90        100        110        120 
PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS KVLLSICSLL TDPNPDDPLV 

       130        140 
PEIAHMYKTD KNKYESTARS WTQKYAMG 

« Hide

References

« Hide 'large scale' references
[1]"Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Columbia.
Tissue: Leaf.
[2]"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[3]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner."
Disch S., Anastasiou E., Sharma V.K., Laux T., Fletcher J.C., Lenhard M.
Curr. Biol. 16:272-279(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BB.
[8]"AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
Luo J., Shen G., Yan J., He C., Zhang H.
Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHIP.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z14991 mRNA. Translation: CAA78715.1.
L00640 mRNA. Translation: AAA32895.1.
Z14993 Genomic DNA. No translation available.
DQ027024 mRNA. Translation: AAY44850.1.
AB013388 Genomic DNA. Translation: BAB09792.1.
CP002688 Genomic DNA. Translation: AED96334.1.
CP002688 Genomic DNA. Translation: AED96335.1.
CP002688 Genomic DNA. Translation: AED96337.1.
AF324718 mRNA. Translation: AAG40069.1.
AF325005 mRNA. Translation: AAG40357.1.
AF326872 mRNA. Translation: AAG41454.1.
AY039566 mRNA. Translation: AAK62621.1.
AY065059 mRNA. Translation: AAL57693.1.
AY113937 mRNA. Translation: AAM44985.1.
AY129488 mRNA. Translation: AAM91074.1.
AY086447 mRNA. Translation: AAM63450.1.
PIRS32672.
RefSeqNP_001190528.1. NM_001203599.1.
NP_568788.1. NM_124709.2.
NP_851181.1. NM_180850.2.
UniGeneAt.24357.

3D structure databases

ProteinModelPortalP35133.
SMRP35133. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20656. 10 interactions.

Proteomic databases

PaxDbP35133.
PRIDEP35133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G53300.1; AT5G53300.1; AT5G53300. [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300. [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300. [P35133-1]
GeneID835411.
KEGGath:AT5G53300.

Organism-specific databases

TAIRAT5G53300.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233455.
InParanoidP35133.
KOK06689.
OMATTKIYHC.
PhylomeDBP35133.
ProtClustDBCLSN2690003.

Enzyme and pathway databases

BioCycARA:AT5G53300-MONOMER.
ARA:GQT-1310-MONOMER.
ARA:GQT-1311-MONOMER.
ARA:GQT-1312-MONOMER.
UniPathwayUPA00143.

Gene expression databases

GenevestigatorP35133.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBC10_ARATH
AccessionPrimary (citable) accession number: P35133
Secondary accession number(s): P56617, Q4TZ00, Q8LCR4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names