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P35133

- UBC10_ARATH

UniProt

P35133 - UBC10_ARATH

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Protein
Ubiquitin-conjugating enzyme E2 10
Gene
UBC10, UBC12, At5g53300, K19E1.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. ubiquitin protein ligase binding Source: UniProtKB
  4. ubiquitin-protein transferase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G53300-MONOMER.
ARA:GQT-1310-MONOMER.
ARA:GQT-1311-MONOMER.
ARA:GQT-1312-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 10 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 10/12
Ubiquitin-conjugating enzyme E2-17 kDa 10/12
Ubiquitin-protein ligase 10/12
Gene namesi
Name:UBC10
Synonyms:UBC12
Ordered Locus Names:At5g53300
ORF Names:K19E1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G53300.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 10
PRO_0000082578Add
BLAST

Proteomic databases

PaxDbiP35133.
PRIDEiP35133.

Expressioni

Tissue specificityi

Ubiquitously expressed with the highest levels in rosette leaves, roots and petals.1 Publication

Gene expression databases

GenevestigatoriP35133.

Interactioni

Subunit structurei

Interacts with CHIP and the E3 ubiquitin ligase BB.2 Publications

Protein-protein interaction databases

BioGridi20656. 10 interactions.

Structurei

3D structure databases

ProteinModelPortaliP35133.
SMRiP35133. Positions 1-147.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiP35133.
KOiK06689.
OMAiSAGPCED.
PhylomeDBiP35133.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P35133-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP SESPYAGGVF    50
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS 100
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD KNKYESTARS WTQKYAMG 148
Length:148
Mass (Da):16,537
Last modified:February 1, 1994 - v1
Checksum:i2B83EDC1AD2AE657
GO

Sequence cautioni

The sequence Z14993 differs from that shown. Reason: Artifacts of PCR amplification. Originally thought to be UBC12 isoform.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231A → T in Z14993. 1 Publication
Sequence conflicti140 – 1401S → T in Z14993. 1 Publication
Sequence conflicti144 – 1441K → E in AAM63450. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14991 mRNA. Translation: CAA78715.1.
L00640 mRNA. Translation: AAA32895.1.
Z14993 Genomic DNA. No translation available.
DQ027024 mRNA. Translation: AAY44850.1.
AB013388 Genomic DNA. Translation: BAB09792.1.
CP002688 Genomic DNA. Translation: AED96334.1.
CP002688 Genomic DNA. Translation: AED96335.1.
CP002688 Genomic DNA. Translation: AED96337.1.
AF324718 mRNA. Translation: AAG40069.1.
AF325005 mRNA. Translation: AAG40357.1.
AF326872 mRNA. Translation: AAG41454.1.
AY039566 mRNA. Translation: AAK62621.1.
AY065059 mRNA. Translation: AAL57693.1.
AY113937 mRNA. Translation: AAM44985.1.
AY129488 mRNA. Translation: AAM91074.1.
AY086447 mRNA. Translation: AAM63450.1.
PIRiS32672.
RefSeqiNP_001190528.1. NM_001203599.1. [P35133-1]
NP_568788.1. NM_124709.2. [P35133-1]
NP_851181.1. NM_180850.2. [P35133-1]
UniGeneiAt.24357.

Genome annotation databases

EnsemblPlantsiAT5G53300.1; AT5G53300.1; AT5G53300. [P35133-1]
AT5G53300.2; AT5G53300.2; AT5G53300. [P35133-1]
AT5G53300.4; AT5G53300.4; AT5G53300. [P35133-1]
GeneIDi835411.
KEGGiath:AT5G53300.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14991 mRNA. Translation: CAA78715.1 .
L00640 mRNA. Translation: AAA32895.1 .
Z14993 Genomic DNA. No translation available.
DQ027024 mRNA. Translation: AAY44850.1 .
AB013388 Genomic DNA. Translation: BAB09792.1 .
CP002688 Genomic DNA. Translation: AED96334.1 .
CP002688 Genomic DNA. Translation: AED96335.1 .
CP002688 Genomic DNA. Translation: AED96337.1 .
AF324718 mRNA. Translation: AAG40069.1 .
AF325005 mRNA. Translation: AAG40357.1 .
AF326872 mRNA. Translation: AAG41454.1 .
AY039566 mRNA. Translation: AAK62621.1 .
AY065059 mRNA. Translation: AAL57693.1 .
AY113937 mRNA. Translation: AAM44985.1 .
AY129488 mRNA. Translation: AAM91074.1 .
AY086447 mRNA. Translation: AAM63450.1 .
PIRi S32672.
RefSeqi NP_001190528.1. NM_001203599.1. [P35133-1 ]
NP_568788.1. NM_124709.2. [P35133-1 ]
NP_851181.1. NM_180850.2. [P35133-1 ]
UniGenei At.24357.

3D structure databases

ProteinModelPortali P35133.
SMRi P35133. Positions 1-147.
ModBasei Search...

Protein-protein interaction databases

BioGridi 20656. 10 interactions.

Proteomic databases

PaxDbi P35133.
PRIDEi P35133.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G53300.1 ; AT5G53300.1 ; AT5G53300 . [P35133-1 ]
AT5G53300.2 ; AT5G53300.2 ; AT5G53300 . [P35133-1 ]
AT5G53300.4 ; AT5G53300.4 ; AT5G53300 . [P35133-1 ]
GeneIDi 835411.
KEGGi ath:AT5G53300.

Organism-specific databases

TAIRi AT5G53300.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
InParanoidi P35133.
KOi K06689.
OMAi SAGPCED.
PhylomeDBi P35133.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G53300-MONOMER.
ARA:GQT-1310-MONOMER.
ARA:GQT-1311-MONOMER.
ARA:GQT-1312-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

Genevestigatori P35133.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
    Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
    Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner."
    Disch S., Anastasiou E., Sharma V.K., Laux T., Fletcher J.C., Lenhard M.
    Curr. Biol. 16:272-279(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BB.
  8. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
    Luo J., Shen G., Yan J., He C., Zhang H.
    Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHIP.

Entry informationi

Entry nameiUBC10_ARATH
AccessioniPrimary (citable) accession number: P35133
Secondary accession number(s): P56617, Q4TZ00, Q8LCR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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