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Protein

Ubiquitin-conjugating enzyme E2 8

Gene

UBC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • endosperm development Source: TAIR
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.19 399
ReactomeiR-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-5689896 Ovarian tumor domain proteases
R-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-9033241 Peroxisomal protein import
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 8 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 8
UBCAT4A
Ubiquitin carrier protein 8
Ubiquitin-conjugating enzyme E2-17 kDa 8
Ubiquitin-protein ligase 8
Gene namesi
Name:UBC8
Synonyms:UBC4A
Ordered Locus Names:At5g41700
ORF Names:MBK23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G41700
TAIRilocus:2160462 AT5G41700

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825761 – 148Ubiquitin-conjugating enzyme E2 8Add BLAST148

Proteomic databases

PaxDbiP35131
PRIDEiP35131

Expressioni

Tissue specificityi

Highest expression in young stems, old leaves. Lowest levels in floral buds, anthers and young leaves.1 Publication

Developmental stagei

Up-regulated during senescence, but not during the G0 to S phase transition.1 Publication

Inductioni

Not induced by heat shock or wounding.1 Publication

Gene expression databases

ExpressionAtlasiP35131 baseline and differential
GenevisibleiP35131 AT

Interactioni

Subunit structurei

Interacts with CIP8, CHIP, NLA and XERICO.4 Publications

Protein-protein interaction databases

BioGridi19424, 17 interactors
IntActiP35131, 2 interactors
STRINGi3702.AT5G41700.4

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Beta strandi21 – 28Combined sources8
Beta strandi32 – 38Combined sources7
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 61Combined sources4
Beta strandi66 – 69Combined sources4
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X57X-ray2.80A/C1-148[»]
ProteinModelPortaliP35131
SMRiP35131
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
InParanoidiP35131
OMAiPPTSCIF
PhylomeDBiP35131

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35131-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP AESPYSGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYEATARN WTQKYAMG
Length:148
Mass (Da):16,533
Last modified:February 1, 1994 - v1
Checksum:i27CAAEABBBE74972
GO
Isoform 2 (identifier: P35131-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-22: CS → CIF

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:149
Mass (Da):16,706
Checksum:i11D4DF7CB733D0B8
GO
Isoform 3 (identifier: P35131-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-148: LLSICSLLTD...RNWTQKYAMG → TLIFQKHRFS...QALLLECLCM

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:145
Mass (Da):16,163
Checksum:i4727400F23743DDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72K → M in AAL34248 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAK44072 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAL66929 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAL15262 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAK96786 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAG40361 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAK44072 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAL66929 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAL15262 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAK96786 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAG40361 (PubMed:14593172).Curated1
Sequence conflicti135E → K in AAM62889 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03492521 – 22CS → CIF in isoform 2. Curated2
Alternative sequenceiVSP_034926103 – 148LLSIC…KYAMG → TLIFQKHRFSNVRLCSKSIA RASTFSARILLNAQALLLEC LCM in isoform 3. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14989 Genomic DNA Translation: CAA78713.1
DQ027022 mRNA Translation: AAY44848.1
AB005233 Genomic DNA Translation: BAB11476.1
CP002688 Genomic DNA Translation: AED94711.1
CP002688 Genomic DNA Translation: AED94712.1
CP002688 Genomic DNA Translation: AED94713.1
CP002688 Genomic DNA Translation: AED94714.1
CP002688 Genomic DNA Translation: AED94715.1
AF325009 mRNA Translation: AAG40361.1
AY063074 mRNA Translation: AAL34248.1
AF370257 mRNA Translation: AAK44072.1
AY054595 mRNA Translation: AAK96786.1
AY057631 mRNA Translation: AAL15262.1
AY072514 mRNA Translation: AAL66929.1
AY085670 mRNA Translation: AAM62889.1
Z37225 mRNA Translation: CAA85527.1
Z17692 mRNA Translation: CAA79036.1
RefSeqiNP_001190447.1, NM_001203518.1 [P35131-1]
NP_568595.2, NM_123535.2 [P35131-2]
NP_851114.1, NM_180783.2 [P35131-1]
NP_851115.1, NM_180784.2 [P35131-1]
NP_851116.1, NM_180785.2 [P35131-3]
UniGeneiAt.23971
At.24180

Genome annotation databases

EnsemblPlantsiAT5G41700.1; AT5G41700.1; AT5G41700 [P35131-1]
AT5G41700.2; AT5G41700.2; AT5G41700 [P35131-1]
AT5G41700.3; AT5G41700.3; AT5G41700 [P35131-3]
AT5G41700.4; AT5G41700.4; AT5G41700 [P35131-2]
AT5G41700.5; AT5G41700.5; AT5G41700 [P35131-1]
GeneIDi834173
GrameneiAT5G41700.1; AT5G41700.1; AT5G41700 [P35131-1]
AT5G41700.2; AT5G41700.2; AT5G41700 [P35131-1]
AT5G41700.3; AT5G41700.3; AT5G41700 [P35131-3]
AT5G41700.4; AT5G41700.4; AT5G41700 [P35131-2]
AT5G41700.5; AT5G41700.5; AT5G41700 [P35131-1]
KEGGiath:AT5G41700

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiUBC8_ARATH
AccessioniPrimary (citable) accession number: P35131
Secondary accession number(s): Q3E8J2
, Q3E8J3, Q42308, Q43276, Q4TZ02, Q8LE19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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