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P35131

- UBC8_ARATH

UniProt

P35131 - UBC8_ARATH

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Protein
Ubiquitin-conjugating enzyme E2 8
Gene
UBC8, UBC4A, At5g41700, MBK23.24
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. acid-amino acid ligase activity Source: InterPro
  3. protein binding Source: TAIR
  4. ubiquitin-protein transferase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. endosperm development Source: TAIR
  2. protein ubiquitination Source: GOC
  3. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G41700-MONOMER.
ARA:GQT-251-MONOMER.
ARA:GQT-252-MONOMER.
ARA:GQT-253-MONOMER.
ARA:GQT-254-MONOMER.
ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 8 (EC:6.3.2.19)
Alternative name(s):
UBCAT4A
Ubiquitin carrier protein 8
Ubiquitin-conjugating enzyme E2-17 kDa 8
Ubiquitin-protein ligase 8
Gene namesi
Name:UBC8
Synonyms:UBC4A
Ordered Locus Names:At5g41700
ORF Names:MBK23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G41700.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 8
PRO_0000082576Add
BLAST

Proteomic databases

PRIDEiP35131.

Expressioni

Tissue specificityi

Highest expression in young stems, old leaves. Lowest levels in floral buds, anthers and young leaves.1 Publication

Developmental stagei

Up-regulated during senescence, but not during the G0 to S phase transition.1 Publication

Inductioni

Not induced by heat shock or wounding.1 Publication

Gene expression databases

GenevestigatoriP35131.

Interactioni

Subunit structurei

Interacts with CIP8, CHIP, NLA and XERICO.4 Publications

Protein-protein interaction databases

BioGridi19424. 14 interactions.
IntActiP35131. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP35131.
SMRiP35131. Positions 1-147.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP35131.
KOiK06689.
OMAiAWAENIA.
PhylomeDBiP35131.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P35131-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP AESPYSGGVF    50
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS 100
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYEATARN WTQKYAMG 148
Length:148
Mass (Da):16,533
Last modified:February 1, 1994 - v1
Checksum:i27CAAEABBBE74972
GO
Isoform 2 (identifier: P35131-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-22: CS → CIF

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:149
Mass (Da):16,706
Checksum:i11D4DF7CB733D0B8
GO
Isoform 3 (identifier: P35131-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-148: LLSICSLLTD...RNWTQKYAMG → TLIFQKHRFS...QALLLECLCM

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:145
Mass (Da):16,163
Checksum:i4727400F23743DDD
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei21 – 222CS → CIF in isoform 2.
VSP_034925
Alternative sequencei103 – 14846LLSIC…KYAMG → TLIFQKHRFSNVRLCSKSIA RASTFSARILLNAQALLLEC LCM in isoform 3.
VSP_034926Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721K → M in AAL34248. 1 Publication
Sequence conflicti80 – 801S → D in AAK44072. 1 Publication
Sequence conflicti80 – 801S → D in AAL66929. 1 Publication
Sequence conflicti80 – 801S → D in AAL15262. 1 Publication
Sequence conflicti80 – 801S → D in AAK96786. 1 Publication
Sequence conflicti80 – 801S → D in AAG40361. 1 Publication
Sequence conflicti98 – 981T → P in AAK44072. 1 Publication
Sequence conflicti98 – 981T → P in AAL66929. 1 Publication
Sequence conflicti98 – 981T → P in AAL15262. 1 Publication
Sequence conflicti98 – 981T → P in AAK96786. 1 Publication
Sequence conflicti98 – 981T → P in AAG40361. 1 Publication
Sequence conflicti135 – 1351E → K in AAM62889. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14989 Genomic DNA. Translation: CAA78713.1.
DQ027022 mRNA. Translation: AAY44848.1.
AB005233 Genomic DNA. Translation: BAB11476.1.
CP002688 Genomic DNA. Translation: AED94711.1.
CP002688 Genomic DNA. Translation: AED94712.1.
CP002688 Genomic DNA. Translation: AED94713.1.
CP002688 Genomic DNA. Translation: AED94714.1.
CP002688 Genomic DNA. Translation: AED94715.1.
AF325009 mRNA. Translation: AAG40361.1.
AY063074 mRNA. Translation: AAL34248.1.
AF370257 mRNA. Translation: AAK44072.1.
AY054595 mRNA. Translation: AAK96786.1.
AY057631 mRNA. Translation: AAL15262.1.
AY072514 mRNA. Translation: AAL66929.1.
AY085670 mRNA. Translation: AAM62889.1.
Z37225 mRNA. Translation: CAA85527.1.
Z17692 mRNA. Translation: CAA79036.1.
RefSeqiNP_001190447.1. NM_001203518.1. [P35131-1]
NP_568595.2. NM_123535.2. [P35131-2]
NP_851114.1. NM_180783.1. [P35131-1]
NP_851115.1. NM_180784.1. [P35131-1]
NP_851116.1. NM_180785.2. [P35131-3]
UniGeneiAt.23971.
At.24180.

Genome annotation databases

EnsemblPlantsiAT5G41700.1; AT5G41700.1; AT5G41700. [P35131-1]
AT5G41700.2; AT5G41700.2; AT5G41700. [P35131-1]
AT5G41700.5; AT5G41700.5; AT5G41700. [P35131-1]
GeneIDi834173.
KEGGiath:AT5G41700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14989 Genomic DNA. Translation: CAA78713.1 .
DQ027022 mRNA. Translation: AAY44848.1 .
AB005233 Genomic DNA. Translation: BAB11476.1 .
CP002688 Genomic DNA. Translation: AED94711.1 .
CP002688 Genomic DNA. Translation: AED94712.1 .
CP002688 Genomic DNA. Translation: AED94713.1 .
CP002688 Genomic DNA. Translation: AED94714.1 .
CP002688 Genomic DNA. Translation: AED94715.1 .
AF325009 mRNA. Translation: AAG40361.1 .
AY063074 mRNA. Translation: AAL34248.1 .
AF370257 mRNA. Translation: AAK44072.1 .
AY054595 mRNA. Translation: AAK96786.1 .
AY057631 mRNA. Translation: AAL15262.1 .
AY072514 mRNA. Translation: AAL66929.1 .
AY085670 mRNA. Translation: AAM62889.1 .
Z37225 mRNA. Translation: CAA85527.1 .
Z17692 mRNA. Translation: CAA79036.1 .
RefSeqi NP_001190447.1. NM_001203518.1. [P35131-1 ]
NP_568595.2. NM_123535.2. [P35131-2 ]
NP_851114.1. NM_180783.1. [P35131-1 ]
NP_851115.1. NM_180784.1. [P35131-1 ]
NP_851116.1. NM_180785.2. [P35131-3 ]
UniGenei At.23971.
At.24180.

3D structure databases

ProteinModelPortali P35131.
SMRi P35131. Positions 1-147.
ModBasei Search...

Protein-protein interaction databases

BioGridi 19424. 14 interactions.
IntActi P35131. 2 interactions.

Proteomic databases

PRIDEi P35131.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G41700.1 ; AT5G41700.1 ; AT5G41700 . [P35131-1 ]
AT5G41700.2 ; AT5G41700.2 ; AT5G41700 . [P35131-1 ]
AT5G41700.5 ; AT5G41700.5 ; AT5G41700 . [P35131-1 ]
GeneIDi 834173.
KEGGi ath:AT5G41700.

Organism-specific databases

TAIRi AT5G41700.

Phylogenomic databases

InParanoidi P35131.
KOi K06689.
OMAi AWAENIA.
PhylomeDBi P35131.

Enzyme and pathway databases

UniPathwayi UPA00143 .
BioCyci ARA:AT5G41700-MONOMER.
ARA:GQT-251-MONOMER.
ARA:GQT-252-MONOMER.
ARA:GQT-253-MONOMER.
ARA:GQT-254-MONOMER.
Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

Genevestigatori P35131.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
    Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
    Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
    Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
    Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-74.
    Strain: cv. Columbia.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-148.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  9. "Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages in Arabidopsis thaliana and Nicotiana sylvestris."
    Genschik P., Durr A., Fleck J.
    Mol. Gen. Genet. 244:548-556(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, INDUCTION.
  10. "Biochemical evidence for ubiquitin ligase activity of the Arabidopsis COP1 interacting protein 8 (CIP8)."
    Hardtke C.S., Okamoto H., Stoop-Myer C., Deng X.-W.
    Plant J. 30:385-394(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CIP8.
  11. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
    Luo J., Shen G., Yan J., He C., Zhang H.
    Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHIP.
  12. "Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis."
    Ko J.-H., Yang S.H., Han K.-H.
    Plant J. 47:343-355(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH XERICO.
  13. "A mutation in NLA, which encodes a RING-type ubiquitin ligase, disrupts the adaptability of Arabidopsis to nitrogen limitation."
    Peng M., Hannam C., Gu H., Bi Y.-M., Rothstein S.J.
    Plant J. 50:320-337(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NLA.

Entry informationi

Entry nameiUBC8_ARATH
AccessioniPrimary (citable) accession number: P35131
Secondary accession number(s): Q3E8J2
, Q3E8J3, Q42308, Q43276, Q4TZ02, Q8LE19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: September 3, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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