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P35131

- UBC8_ARATH

UniProt

P35131 - UBC8_ARATH

Protein

Ubiquitin-conjugating enzyme E2 8

Gene

UBC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: TAIR
    4. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. endosperm development Source: TAIR
    2. protein ubiquitination Source: GOC
    3. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G41700-MONOMER.
    ARA:GQT-251-MONOMER.
    ARA:GQT-252-MONOMER.
    ARA:GQT-253-MONOMER.
    ARA:GQT-254-MONOMER.
    ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 8 (EC:6.3.2.19)
    Alternative name(s):
    UBCAT4A
    Ubiquitin carrier protein 8
    Ubiquitin-conjugating enzyme E2-17 kDa 8
    Ubiquitin-protein ligase 8
    Gene namesi
    Name:UBC8
    Synonyms:UBC4A
    Ordered Locus Names:At5g41700
    ORF Names:MBK23.24
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G41700.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. nucleus Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 148148Ubiquitin-conjugating enzyme E2 8PRO_0000082576Add
    BLAST

    Proteomic databases

    PRIDEiP35131.

    Expressioni

    Tissue specificityi

    Highest expression in young stems, old leaves. Lowest levels in floral buds, anthers and young leaves.1 Publication

    Developmental stagei

    Up-regulated during senescence, but not during the G0 to S phase transition.1 Publication

    Inductioni

    Not induced by heat shock or wounding.1 Publication

    Gene expression databases

    GenevestigatoriP35131.

    Interactioni

    Subunit structurei

    Interacts with CIP8, CHIP, NLA and XERICO.4 Publications

    Protein-protein interaction databases

    BioGridi19424. 14 interactions.
    IntActiP35131. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP35131.
    SMRiP35131. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    InParanoidiP35131.
    KOiK06689.
    OMAiAWAENIA.
    PhylomeDBiP35131.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P35131-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP AESPYSGGVF    50
    LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS 100
    KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYEATARN WTQKYAMG 148
    Length:148
    Mass (Da):16,533
    Last modified:February 1, 1994 - v1
    Checksum:i27CAAEABBBE74972
    GO
    Isoform 2 (identifier: P35131-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         21-22: CS → CIF

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:149
    Mass (Da):16,706
    Checksum:i11D4DF7CB733D0B8
    GO
    Isoform 3 (identifier: P35131-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         103-148: LLSICSLLTD...RNWTQKYAMG → TLIFQKHRFS...QALLLECLCM

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:145
    Mass (Da):16,163
    Checksum:i4727400F23743DDD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721K → M in AAL34248. (PubMed:14593172)Curated
    Sequence conflicti80 – 801S → D in AAK44072. (PubMed:14593172)Curated
    Sequence conflicti80 – 801S → D in AAL66929. (PubMed:14593172)Curated
    Sequence conflicti80 – 801S → D in AAL15262. (PubMed:14593172)Curated
    Sequence conflicti80 – 801S → D in AAK96786. (PubMed:14593172)Curated
    Sequence conflicti80 – 801S → D in AAG40361. (PubMed:14593172)Curated
    Sequence conflicti98 – 981T → P in AAK44072. (PubMed:14593172)Curated
    Sequence conflicti98 – 981T → P in AAL66929. (PubMed:14593172)Curated
    Sequence conflicti98 – 981T → P in AAL15262. (PubMed:14593172)Curated
    Sequence conflicti98 – 981T → P in AAK96786. (PubMed:14593172)Curated
    Sequence conflicti98 – 981T → P in AAG40361. (PubMed:14593172)Curated
    Sequence conflicti135 – 1351E → K in AAM62889. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei21 – 222CS → CIF in isoform 2. CuratedVSP_034925
    Alternative sequencei103 – 14846LLSIC…KYAMG → TLIFQKHRFSNVRLCSKSIA RASTFSARILLNAQALLLEC LCM in isoform 3. CuratedVSP_034926Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14989 Genomic DNA. Translation: CAA78713.1.
    DQ027022 mRNA. Translation: AAY44848.1.
    AB005233 Genomic DNA. Translation: BAB11476.1.
    CP002688 Genomic DNA. Translation: AED94711.1.
    CP002688 Genomic DNA. Translation: AED94712.1.
    CP002688 Genomic DNA. Translation: AED94713.1.
    CP002688 Genomic DNA. Translation: AED94714.1.
    CP002688 Genomic DNA. Translation: AED94715.1.
    AF325009 mRNA. Translation: AAG40361.1.
    AY063074 mRNA. Translation: AAL34248.1.
    AF370257 mRNA. Translation: AAK44072.1.
    AY054595 mRNA. Translation: AAK96786.1.
    AY057631 mRNA. Translation: AAL15262.1.
    AY072514 mRNA. Translation: AAL66929.1.
    AY085670 mRNA. Translation: AAM62889.1.
    Z37225 mRNA. Translation: CAA85527.1.
    Z17692 mRNA. Translation: CAA79036.1.
    RefSeqiNP_001190447.1. NM_001203518.1. [P35131-1]
    NP_568595.2. NM_123535.2. [P35131-2]
    NP_851114.1. NM_180783.1. [P35131-1]
    NP_851115.1. NM_180784.1. [P35131-1]
    NP_851116.1. NM_180785.2. [P35131-3]
    UniGeneiAt.23971.
    At.24180.

    Genome annotation databases

    EnsemblPlantsiAT5G41700.1; AT5G41700.1; AT5G41700. [P35131-1]
    AT5G41700.2; AT5G41700.2; AT5G41700. [P35131-1]
    AT5G41700.5; AT5G41700.5; AT5G41700. [P35131-1]
    GeneIDi834173.
    KEGGiath:AT5G41700.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantsUBQ

    A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14989 Genomic DNA. Translation: CAA78713.1 .
    DQ027022 mRNA. Translation: AAY44848.1 .
    AB005233 Genomic DNA. Translation: BAB11476.1 .
    CP002688 Genomic DNA. Translation: AED94711.1 .
    CP002688 Genomic DNA. Translation: AED94712.1 .
    CP002688 Genomic DNA. Translation: AED94713.1 .
    CP002688 Genomic DNA. Translation: AED94714.1 .
    CP002688 Genomic DNA. Translation: AED94715.1 .
    AF325009 mRNA. Translation: AAG40361.1 .
    AY063074 mRNA. Translation: AAL34248.1 .
    AF370257 mRNA. Translation: AAK44072.1 .
    AY054595 mRNA. Translation: AAK96786.1 .
    AY057631 mRNA. Translation: AAL15262.1 .
    AY072514 mRNA. Translation: AAL66929.1 .
    AY085670 mRNA. Translation: AAM62889.1 .
    Z37225 mRNA. Translation: CAA85527.1 .
    Z17692 mRNA. Translation: CAA79036.1 .
    RefSeqi NP_001190447.1. NM_001203518.1. [P35131-1 ]
    NP_568595.2. NM_123535.2. [P35131-2 ]
    NP_851114.1. NM_180783.1. [P35131-1 ]
    NP_851115.1. NM_180784.1. [P35131-1 ]
    NP_851116.1. NM_180785.2. [P35131-3 ]
    UniGenei At.23971.
    At.24180.

    3D structure databases

    ProteinModelPortali P35131.
    SMRi P35131. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 19424. 14 interactions.
    IntActi P35131. 2 interactions.

    Proteomic databases

    PRIDEi P35131.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G41700.1 ; AT5G41700.1 ; AT5G41700 . [P35131-1 ]
    AT5G41700.2 ; AT5G41700.2 ; AT5G41700 . [P35131-1 ]
    AT5G41700.5 ; AT5G41700.5 ; AT5G41700 . [P35131-1 ]
    GeneIDi 834173.
    KEGGi ath:AT5G41700.

    Organism-specific databases

    TAIRi AT5G41700.

    Phylogenomic databases

    InParanoidi P35131.
    KOi K06689.
    OMAi AWAENIA.
    PhylomeDBi P35131.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    BioCyci ARA:AT5G41700-MONOMER.
    ARA:GQT-251-MONOMER.
    ARA:GQT-252-MONOMER.
    ARA:GQT-253-MONOMER.
    ARA:GQT-254-MONOMER.
    Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_185297. Separation of Sister Chromatids.
    REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
    REACT_208398. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

    Gene expression databases

    Genevestigatori P35131.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Homologs of the essential ubiquitin conjugating enzymes UBC1, 4, and 5 in yeast are encoded by a multigene family in Arabidopsis thaliana."
      Girod P.-A., Carpenter T.B., van Nocker S., Sullivan M.L., Vierstra R.D.
      Plant J. 3:545-552(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
      Tissue: Leaf.
    2. "Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis."
      Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J.
      Plant Physiol. 139:1597-1611(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    3. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-74.
      Strain: cv. Columbia.
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 36-148.
      Strain: cv. Columbia.
      Tissue: Green siliques.
    9. "Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages in Arabidopsis thaliana and Nicotiana sylvestris."
      Genschik P., Durr A., Fleck J.
      Mol. Gen. Genet. 244:548-556(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, INDUCTION.
    10. "Biochemical evidence for ubiquitin ligase activity of the Arabidopsis COP1 interacting protein 8 (CIP8)."
      Hardtke C.S., Okamoto H., Stoop-Myer C., Deng X.-W.
      Plant J. 30:385-394(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CIP8.
    11. "AtCHIP functions as an E3 ubiquitin ligase of protein phosphatase 2A subunits and alters plant response to abscisic acid treatment."
      Luo J., Shen G., Yan J., He C., Zhang H.
      Plant J. 46:649-657(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHIP.
    12. "Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis."
      Ko J.-H., Yang S.H., Han K.-H.
      Plant J. 47:343-355(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH XERICO.
    13. "A mutation in NLA, which encodes a RING-type ubiquitin ligase, disrupts the adaptability of Arabidopsis to nitrogen limitation."
      Peng M., Hannam C., Gu H., Bi Y.-M., Rothstein S.J.
      Plant J. 50:320-337(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NLA.

    Entry informationi

    Entry nameiUBC8_ARATH
    AccessioniPrimary (citable) accession number: P35131
    Secondary accession number(s): Q3E8J2
    , Q3E8J3, Q42308, Q43276, Q4TZ02, Q8LE19
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3