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Protein

Ubiquitin-conjugating enzyme E2 8

Gene

UBC8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • transferase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: TAIR

GO - Biological processi

  • endosperm development Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.19. 399.
ReactomeiR-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-179409. APC-Cdc20 mediated degradation of Nek2A.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 8 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 8
UBCAT4A
Ubiquitin carrier protein 8
Ubiquitin-conjugating enzyme E2-17 kDa 8
Ubiquitin-protein ligase 8
Gene namesi
Name:UBC8
Synonyms:UBC4A
Ordered Locus Names:At5g41700
ORF Names:MBK23.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G41700.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825761 – 148Ubiquitin-conjugating enzyme E2 8Add BLAST148

Proteomic databases

PaxDbiP35131.
PRIDEiP35131.

Expressioni

Tissue specificityi

Highest expression in young stems, old leaves. Lowest levels in floral buds, anthers and young leaves.1 Publication

Developmental stagei

Up-regulated during senescence, but not during the G0 to S phase transition.1 Publication

Inductioni

Not induced by heat shock or wounding.1 Publication

Gene expression databases

ExpressionAtlasiP35131. baseline and differential.
GenevisibleiP35131. AT.

Interactioni

Subunit structurei

Interacts with CIP8, CHIP, NLA and XERICO.4 Publications

Protein-protein interaction databases

BioGridi19424. 15 interactors.
IntActiP35131. 2 interactors.
STRINGi3702.AT5G41700.4.

Structurei

Secondary structure

1148
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 15Combined sources15
Beta strandi21 – 28Combined sources8
Beta strandi32 – 38Combined sources7
Turni44 – 47Combined sources4
Beta strandi49 – 55Combined sources7
Turni58 – 61Combined sources4
Beta strandi66 – 69Combined sources4
Helixi87 – 89Combined sources3
Turni90 – 92Combined sources3
Helixi99 – 111Combined sources13
Helixi121 – 129Combined sources9
Helixi131 – 145Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X57X-ray2.80A/C1-148[»]
ProteinModelPortaliP35131.
SMRiP35131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
InParanoidiP35131.
OMAiTDRKKYN.
PhylomeDBiP35131.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35131-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASKRILKEL KDLQKDPPTS CSAGPVAEDM FHWQATIMGP AESPYSGGVF
60 70 80 90 100
LVTIHFPPDY PFKPPKVAFR TKVFHPNINS NGSICLDILK EQWSPALTIS
110 120 130 140
KVLLSICSLL TDPNPDDPLV PEIAHMYKTD RAKYEATARN WTQKYAMG
Length:148
Mass (Da):16,533
Last modified:February 1, 1994 - v1
Checksum:i27CAAEABBBE74972
GO
Isoform 2 (identifier: P35131-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-22: CS → CIF

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:149
Mass (Da):16,706
Checksum:i11D4DF7CB733D0B8
GO
Isoform 3 (identifier: P35131-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-148: LLSICSLLTD...RNWTQKYAMG → TLIFQKHRFS...QALLLECLCM

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:145
Mass (Da):16,163
Checksum:i4727400F23743DDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72K → M in AAL34248 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAK44072 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAL66929 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAL15262 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAK96786 (PubMed:14593172).Curated1
Sequence conflicti80S → D in AAG40361 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAK44072 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAL66929 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAL15262 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAK96786 (PubMed:14593172).Curated1
Sequence conflicti98T → P in AAG40361 (PubMed:14593172).Curated1
Sequence conflicti135E → K in AAM62889 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03492521 – 22CS → CIF in isoform 2. Curated2
Alternative sequenceiVSP_034926103 – 148LLSIC…KYAMG → TLIFQKHRFSNVRLCSKSIA RASTFSARILLNAQALLLEC LCM in isoform 3. CuratedAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14989 Genomic DNA. Translation: CAA78713.1.
DQ027022 mRNA. Translation: AAY44848.1.
AB005233 Genomic DNA. Translation: BAB11476.1.
CP002688 Genomic DNA. Translation: AED94711.1.
CP002688 Genomic DNA. Translation: AED94712.1.
CP002688 Genomic DNA. Translation: AED94713.1.
CP002688 Genomic DNA. Translation: AED94714.1.
CP002688 Genomic DNA. Translation: AED94715.1.
AF325009 mRNA. Translation: AAG40361.1.
AY063074 mRNA. Translation: AAL34248.1.
AF370257 mRNA. Translation: AAK44072.1.
AY054595 mRNA. Translation: AAK96786.1.
AY057631 mRNA. Translation: AAL15262.1.
AY072514 mRNA. Translation: AAL66929.1.
AY085670 mRNA. Translation: AAM62889.1.
Z37225 mRNA. Translation: CAA85527.1.
Z17692 mRNA. Translation: CAA79036.1.
RefSeqiNP_001190447.1. NM_001203518.1. [P35131-1]
NP_568595.2. NM_123535.2. [P35131-2]
NP_851114.1. NM_180783.2. [P35131-1]
NP_851115.1. NM_180784.2. [P35131-1]
NP_851116.1. NM_180785.2. [P35131-3]
UniGeneiAt.23971.
At.24180.

Genome annotation databases

EnsemblPlantsiAT5G41700.1; AT5G41700.1; AT5G41700. [P35131-1]
AT5G41700.2; AT5G41700.2; AT5G41700. [P35131-1]
AT5G41700.5; AT5G41700.5; AT5G41700. [P35131-1]
GeneIDi834173.
GrameneiAT5G41700.1; AT5G41700.1; AT5G41700.
AT5G41700.2; AT5G41700.2; AT5G41700.
AT5G41700.5; AT5G41700.5; AT5G41700.
KEGGiath:AT5G41700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z14989 Genomic DNA. Translation: CAA78713.1.
DQ027022 mRNA. Translation: AAY44848.1.
AB005233 Genomic DNA. Translation: BAB11476.1.
CP002688 Genomic DNA. Translation: AED94711.1.
CP002688 Genomic DNA. Translation: AED94712.1.
CP002688 Genomic DNA. Translation: AED94713.1.
CP002688 Genomic DNA. Translation: AED94714.1.
CP002688 Genomic DNA. Translation: AED94715.1.
AF325009 mRNA. Translation: AAG40361.1.
AY063074 mRNA. Translation: AAL34248.1.
AF370257 mRNA. Translation: AAK44072.1.
AY054595 mRNA. Translation: AAK96786.1.
AY057631 mRNA. Translation: AAL15262.1.
AY072514 mRNA. Translation: AAL66929.1.
AY085670 mRNA. Translation: AAM62889.1.
Z37225 mRNA. Translation: CAA85527.1.
Z17692 mRNA. Translation: CAA79036.1.
RefSeqiNP_001190447.1. NM_001203518.1. [P35131-1]
NP_568595.2. NM_123535.2. [P35131-2]
NP_851114.1. NM_180783.2. [P35131-1]
NP_851115.1. NM_180784.2. [P35131-1]
NP_851116.1. NM_180785.2. [P35131-3]
UniGeneiAt.23971.
At.24180.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4X57X-ray2.80A/C1-148[»]
ProteinModelPortaliP35131.
SMRiP35131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19424. 15 interactors.
IntActiP35131. 2 interactors.
STRINGi3702.AT5G41700.4.

Proteomic databases

PaxDbiP35131.
PRIDEiP35131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G41700.1; AT5G41700.1; AT5G41700. [P35131-1]
AT5G41700.2; AT5G41700.2; AT5G41700. [P35131-1]
AT5G41700.5; AT5G41700.5; AT5G41700. [P35131-1]
GeneIDi834173.
GrameneiAT5G41700.1; AT5G41700.1; AT5G41700.
AT5G41700.2; AT5G41700.2; AT5G41700.
AT5G41700.5; AT5G41700.5; AT5G41700.
KEGGiath:AT5G41700.

Organism-specific databases

TAIRiAT5G41700.

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
InParanoidiP35131.
OMAiTDRKKYN.
PhylomeDBiP35131.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi6.3.2.19. 399.
ReactomeiR-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-179409. APC-Cdc20 mediated degradation of Nek2A.
R-ATH-5689896. Ovarian tumor domain proteases.
R-ATH-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.

Miscellaneous databases

PROiP35131.

Gene expression databases

ExpressionAtlasiP35131. baseline and differential.
GenevisibleiP35131. AT.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC8_ARATH
AccessioniPrimary (citable) accession number: P35131
Secondary accession number(s): Q3E8J2
, Q3E8J3, Q42308, Q43276, Q4TZ02, Q8LE19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.