Reviewed, UniProtKB/Swiss-Prot P35130 (UBC2_MEDSA)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 2
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-conjugating enzyme E2-17 kDa 2
    Ubiquitin-protein ligase 2
    Ubiquitin carrier protein 2

Gene names

Name:

UBC2

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Ubiquitin-conjugating enzyme E2 2		PRO_0000082574
Sites
	Active site	88	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35130-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 3951D40CF5CE8E24	FASTA	152	17,315
	10 20 30 40 50 60 MSTPARKRLM RDFKRLQHDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG GTFKLSLQFS 70 80 90 100 110 120 EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAILTSIQ SLLCDPNPNS 130 140 150 PANSEAARMF SENKREYNRR VREVVEQSWT AD

References

[1]	"A ubiquitin carrier protein cDNA from developing alfalfa embryos." Pramanik S.K., Bewley J.D. Plant Physiol. 102:1049-1050(1993) [PubMed: 8278526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Excalibur. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L06967 mRNA. Translation: AAA18528.1.
3D structure databases
HSSP	HSSP built from PDB template 2AAK based on UniProtKB P25865.
SMR	P35130. Positions 1-150.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.2.19. 815.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC2_MEDSA

Accession

Primary (citable) accession number: P35130

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)